Restriction map for
pFN21AE9397
(Circular) (Six-base) MAPSORT of: pFN21AE9397.seq Check: 6347 from: 1 to: 21313
pFN21AE9397 21313 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 4129 9500 9865 11443 11592 11945 14324 14406
Size: 1740 5371 365 1578 149 353 2379 82
Cuts at: 14406 15088 2389
Size: 682 8614
Fragments arranged by size:
8614 5371 2379 1740 1578 682 365 353
149 82
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 19123 278
Size: 53 83 186 936 17587 2468
Fragments arranged by size:
17587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2230 5508 8408 14088 17337 17466 18656 1937
Size: 293 3278 2900 5680 3249 129 1190 4594
Fragments arranged by size:
5680 4594 3278 3249 2900 1190 293 129
Acc65I G'GTAC_C
Cuts at: 9989 12103 15184 15512 17074 18634 9989
Size: 2114 3081 328 1562 1560 12668
Fragments arranged by size:
12668 3081 2114 1562 1560 328
AclI AA'CG_TT
Cuts at: 1563 18612 19440 19813 1563
Size: 17049 828 373 3063
Fragments arranged by size:
17049 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 3645 3726 3780 3942 5072 7260 7994
Size: 509 1504 81 54 162 1130 2188 734
Cuts at: 7994 8450 9318 9447 10776 12596 13071 13613 13685
Size: 456 868 129 1329 1820 475 542 72
Cuts at: 13685 13721 13763 14088 15957 15990 16592 16628 16755
Size: 36 42 325 1869 33 602 36 127
Cuts at: 16755 16926 17885 19376 20760 21028 1632
Size: 171 959 1491 1384 268 1917
Fragments arranged by size:
2188 1917 1869 1820 1504 1491 1384 1329
1130 959 868 734 602 542 509 475
456 325 268 171 162 129 127 81
72 54 42 36 36 33
AfeI AGC'GCT
Cuts at: 2516 8015 13672 2516
Size: 5499 5657 10157
Fragments arranged by size:
10157 5657 5499
AflII C'TTAA_G
Cuts at: 820 1017 14641 820
Size: 197 13624 7492
Fragments arranged by size:
13624 7492 197
AflIII A'CryG_T
Cuts at: 1203 2113 2882 6135 7299 17529 18195 18354 18376
Size: 910 769 3253 1164 10230 666 159 22
Cuts at: 18376 20212 21189 1203
Size: 1836 977 1327
Fragments arranged by size:
10230 3253 1836 1327 1164 977 910 769
666 159 22
AgeI A'CCGG_T
Cuts at: 5575 20144 5575
Size: 14569 6744
AhdI GACnn_n'nnGTC
Cuts at: 4912 20042 4912
Size: 15130 6183
AleI CACnn'nnGTG
Cuts at: 1166 5818 6245 8949 17204 1166
Size: 4652 427 2704 8255 5275
Fragments arranged by size:
8255 5275 4652 2704 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6812 10115 12425 12660 16599 17075 17336 1184
Size: 5628 3303 2310 235 3939 476 261 5161
Fragments arranged by size:
5628 5161 3939 3303 2310 476 261 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7286 7318 12527 12559 7286
Size: 32 5209 32 16040
Fragments arranged by size:
16040 5209 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2020 2072 3134 5246 5414 5804 6785 7538 7720
Size: 52 1062 2112 168 390 981 753 182
Cuts at: 7720 7917 8165 9950 10130 10592 10607 11687 11873
Size: 197 248 1785 180 462 15 1080 186
Cuts at: 11873 11986 12205 12677 13201 13369 13639 13717 14531
Size: 113 219 472 524 168 270 78 814
Cuts at: 14531 14554 15374 15676 15747 16367 16484 16556 17310
Size: 23 820 302 71 620 117 72 754
Cuts at: 17310 17575 17749 18226 19056 20628 2020
Size: 265 174 477 830 1572 2705
Fragments arranged by size:
2705 2112 1785 1572 1080 1062 981 830
820 814 754 753 620 524 477 472
462 390 302 270 265 248 219 197
186 182 180 174 168 168 117 113
78 72 71 52 23 15
ApaI G_GGCC'C
Cuts at: 2319 2468 3237 5986 6501 7007 8617 8904 9705
Size: 149 769 2749 515 506 1610 287 801
Cuts at: 9705 9789 10700 11288 13395 13412 13460 13469 14124
Size: 84 911 588 2107 17 48 9 655
Cuts at: 14124 14465 14858 16210 16282 17424 2319
Size: 341 393 1352 72 1142 6208
Fragments arranged by size:
6208 2749 2107 1610 1352 1142 911 801
769 655 588 515 506 393 341 287
149 84 72 48 17 9
ApaLI G'TGCA_C
Cuts at: 5571 17174 18143 19370 20526 5571
Size: 11603 969 1227 1156 6358
Fragments arranged by size:
11603 6358 1227 1156 969
ApoI r'AATT_y
Cuts at: 1493 6116 8675 10569 12242 18622 18848 19000 21109
Size: 4623 2559 1894 1673 6380 226 152 2109
Cuts at: 21109 1493
Size: 1697
Fragments arranged by size:
6380 4623 2559 2109 1894 1697 1673 226
152
AseI AT'TA_AT
Cuts at: 160 1032 8079 19867 160
Size: 872 7047 11788 1606
Fragments arranged by size:
11788 7047 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 21313
AvaI C'yCGr_G
Cuts at: 1870 1943 4739 5474 5757 7511 9000 9028 9228
Size: 73 2796 735 283 1754 1489 28 200
Cuts at: 9228 10973 11645 12388 13497 15258 15975 16236 16473
Size: 1745 672 743 1109 1761 717 261 237
Cuts at: 16473 17927 18638 1870
Size: 1454 711 4545
Fragments arranged by size:
4545 2796 1761 1754 1745 1489 1454 1109
743 735 717 711 672 283 261 237
200 73 28
AvrII C'CTAG_G
Cuts at: 2389 5356 10549 2389
Size: 2967 5193 13153
Fragments arranged by size:
13153 5193 2967
BamHI G'GATC_C
Cuts at: 9869 10969 11618 13070 15133 18643 19010 9869
Size: 1100 649 1452 2063 3510 367 12172
Fragments arranged by size:
12172 3510 2063 1452 1100 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2540 4889 6694 6718 6834
Size: 325 217 627 753 2349 1805 24 116
Cuts at: 6834 7217 7592 7934 8449 8500 8575 8639 8696
Size: 383 375 342 515 51 75 64 57
Cuts at: 8696 8939 9076 9587 9989 11078 11957 12103 12149
Size: 243 137 511 402 1089 879 146 46
Cuts at: 12149 12340 15184 15220 15325 15512 16765 17074 17297
Size: 191 2844 36 105 187 1253 309 223
Cuts at: 17297 17967 18284 18634 20089 618
Size: 670 317 350 1455 1842
Fragments arranged by size:
2844 2349 1842 1805 1455 1253 1089 879
753 670 627 515 511 402 383 375
350 342 325 317 309 243 223 217
191 187 146 137 116 105 75 64
57 51 46 36 24
BanII G_rGCy'C
Cuts at: 1391 2226 2312 2319 2419 2468 2993 3159 3237
Size: 835 86 7 100 49 525 166 78
Cuts at: 3237 3288 5061 5543 5679 5690 5771 5959 5986
Size: 51 1773 482 136 11 81 188 27
Cuts at: 5986 6501 6747 6894 7007 7703 7908 7928 8447
Size: 515 246 147 113 696 205 20 519
Cuts at: 8447 8478 8562 8617 8783 8814 8904 9021 9029
Size: 31 84 55 166 31 90 117 8
Cuts at: 9029 9519 9705 9789 10173 10700 11288 11430 11646
Size: 490 186 84 384 527 588 142 216
Cuts at: 11646 11717 11906 12119 13395 13412 13460 13469 13688
Size: 71 189 213 1276 17 48 9 219
Cuts at: 13688 14044 14124 14465 14858 15286 15605 16181 16210
Size: 356 80 341 393 428 319 576 29
Cuts at: 16210 16282 16469 16550 16652 16797 17424 17444 17880
Size: 72 187 81 102 145 627 20 436
Cuts at: 17880 18234 18632 1391
Size: 354 398 4072
Fragments arranged by size:
4072 1773 1276 835 696 627 588 576
527 525 519 515 490 482 436 428
398 393 384 356 354 341 319 246
219 216 213 205 189 188 187 186
166 166 147 145 142 136 117 113
102 100 90 86 84 84 81 81
80 78 72 71 55 51 49 48
31 31 29 27 20 20 17 11
9 8 7
BbeI G_GCGC'C
Cuts at: 6698 6838 7221 8643 17971 6698
Size: 140 383 1422 9328 10040
Fragments arranged by size:
10040 9328 1422 383 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5370 5941 8000 8971 9945 10778 10829
Size: 971 3471 571 2059 971 974 833 51
Cuts at: 10829 12956 16246 17446 17755 928
Size: 2127 3290 1200 309 4486
Fragments arranged by size:
4486 3471 3290 2127 2059 1200 974 971
971 833 571 309 51
BbvCI CC'TCA_GC
Cuts at: 4299 4380 4401 4785 5856 9657 10804 13962 14253
Size: 81 21 384 1071 3801 1147 3158 291
Cuts at: 14253 14649 15773 16506 4299
Size: 396 1124 733 9106
Fragments arranged by size:
9106 3801 3158 1147 1124 1071 733 396
384 291 81 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2518 2552 3143 3177 10181 10215 10976
Size: 34 581 34 591 34 7004 34 761
Cuts at: 10976 11010 15239 15273 19504 19538 1903
Size: 34 4229 34 4231 34 3678
Fragments arranged by size:
7004 4231 4229 3678 761 591 581 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 11488 15994 18227 19205 20415 2888
Size: 8600 4506 2233 978 1210 3786
Fragments arranged by size:
8600 4506 3786 2233 1210 978
BclI T'GATC_A
Cuts at: 1548 12504 17997 1548
Size: 10956 5493 4864
Fragments arranged by size:
10956 5493 4864
BfrBI ATG'CAT
Cuts at: 6592 17407 6592
Size: 10815 10498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2984 4850 6864
Size: 107 122 71 631 605 1312 1866 2014
Cuts at: 6864 7515 7746 9440 9502 10814 11003 13350 13421
Size: 651 231 1694 62 1312 189 2347 71
Cuts at: 13421 13464 14772 15647 19924 136
Size: 43 1308 875 4277 1525
Fragments arranged by size:
4277 2347 2014 1866 1694 1525 1312 1312
1308 875 651 631 605 231 189 122
107 71 71 62 43
BglII A'GATC_T
Cuts at: 5625 21308 5625
Size: 15683 5630
BlpI GC'TnA_GC
Cuts at: 2992 3090 5208 7234 7613 9138 10167 13674 14406
Size: 98 2118 2026 379 1525 1029 3507 732
Cuts at: 14406 16421 16827 17438 18730 2992
Size: 2015 406 611 1292 5575
Fragments arranged by size:
5575 3507 2118 2026 2015 1525 1292 1029
732 611 406 379 98
Bme1580I G_kGCm'C
Cuts at: 1790 2319 2468 2816 3204 3237 5575 5986 6501
Size: 529 149 348 388 33 2338 411 515
Cuts at: 6501 6723 7007 7572 8338 8505 8617 8904 9590
Size: 222 284 565 766 167 112 287 686
Cuts at: 9590 9705 9789 10679 10700 11288 11510 11535 11962
Size: 115 84 890 21 588 222 25 427
Cuts at: 11962 12152 13395 13412 13460 13469 14124 14394 14465
Size: 190 1243 17 48 9 655 270 71
Cuts at: 14465 14858 15277 15328 15471 15994 16210 16282 16897
Size: 393 419 51 143 523 216 72 615
Cuts at: 16897 17178 17191 17230 17424 18019 18147 18436 19374
Size: 281 13 39 194 595 128 289 938
Cuts at: 19374 20530 1790
Size: 1156 2573
Fragments arranged by size:
2573 2338 1243 1156 938 890 766 686
655 615 595 588 565 529 523 515
427 419 411 393 388 348 289 287
284 281 270 222 222 216 194 190
167 149 143 128 115 112 84 72
71 51 48 39 33 25 21 17
13 9
BmgBI CAC'GTC
Cuts at: 1318 2883 3378 4044 5969 1318
Size: 1565 495 666 1925 16662
Fragments arranged by size:
16662 1925 1565 666 495
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 2331 3283
Size: 416 53 83 399 24 803 111 952
Cuts at: 3283 4504 5021 5119 5262 6039 6742 7559 7566
Size: 1221 517 98 143 777 703 817 7
Cuts at: 7566 8348 9247 9259 10621 10673 11187 11907 13486
Size: 782 899 12 1362 52 514 720 1579
Cuts at: 13486 15280 15438 15746 16244 19044 20002 442
Size: 1794 158 308 498 2800 958 1753
Fragments arranged by size:
2800 1794 1753 1579 1362 1221 958 952
899 817 803 782 777 720 703 517
514 498 416 399 308 158 143 111
98 83 53 52 24 12 7
BmtI G_CTAG'C
Cuts at: 1056 12377 17502 1056
Size: 11321 5125 4867
Fragments arranged by size:
11321 5125 4867
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 11566 14140 14618 17458 3049
Size: 8517 2574 478 2840 6904
Fragments arranged by size:
8517 6904 2840 2574 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 2696 3491 3569 4184 4958 5001 5676
Size: 1182 275 795 78 615 774 43 675
Cuts at: 5676 6693 7019 7313 7795 8167 8576 8978 10052
Size: 1017 326 294 482 372 409 402 1074
Cuts at: 10052 10134 11114 12002 13305 13467 14058 14522 14744
Size: 82 980 888 1303 162 591 464 222
Cuts at: 14744 14807 15515 16310 17771 18478 19973 1239
Size: 63 708 795 1461 707 1495 2579
Fragments arranged by size:
2579 1495 1461 1303 1182 1074 1017 980
888 795 795 774 708 707 675 615
591 482 464 409 402 372 326 294
275 222 162 82 78 63 43
Bpu10I CC'TnA_GC
Cuts at: 4299 4380 4401 4563 4785 4983 5856 7352 7927
Size: 81 21 162 222 198 873 1496 575
Cuts at: 7927 9657 10016 10804 11166 12321 12495 13962 14253
Size: 1730 359 788 362 1155 174 1467 291
Cuts at: 14253 14649 15773 16180 16506 4299
Size: 396 1124 407 326 9106
Fragments arranged by size:
9106 1730 1496 1467 1155 1124 873 788
575 407 396 362 359 326 291 222
198 174 162 81 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3302 3620 4589 6242 6938 8078 8468 12677 14928
Size: 318 969 1653 696 1140 390 4209 2251
Cuts at: 14928 16648 16922 19438 20303 20601 3302
Size: 1720 274 2516 865 298 4014
Fragments arranged by size:
4209 4014 2516 2251 1720 1653 1140 969
865 696 390 318 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2131 5200 5740 7034 9453 9671 10702
Size: 617 632 3069 540 1294 2419 218 1031
Cuts at: 10702 11090 14950 15079 17207 18107 19976 882
Size: 388 3860 129 2128 900 1869 2219
Fragments arranged by size:
3860 3069 2419 2219 2128 1869 1294 1031
900 632 617 540 388 218 129
BsaAI yAC'GTr
Cuts at: 493 1204 3205 5570 6544 6679 19104 493
Size: 711 2001 2365 974 135 12425 2702
Fragments arranged by size:
12425 2702 2365 2001 974 711 135
BsaBI GATnn'nnATC
Cuts at: 21307 21307
Size: 21313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3605 5938 6695 6835
Size: 53 83 186 936 2072 2333 757 140
Cuts at: 6835 7218 8640 11380 15924 17772 17968 19120 19502
Size: 383 1422 2740 4544 1848 196 1152 382
Cuts at: 19502 20137 275
Size: 635 1451
Fragments arranged by size:
4544 2740 2333 2072 1848 1451 1422 1152
936 757 635 383 382 196 186 140
83 53
BsaWI w'CCGG_w
Cuts at: 2188 3571 3784 4135 5575 9558 12744 19746 20144
Size: 1383 213 351 1440 3983 3186 7002 398
Cuts at: 20144 20418 20565 20976 2188
Size: 274 147 411 2525
Fragments arranged by size:
7002 3983 3186 2525 1440 1383 411 398
351 274 213 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4033 4063 4736 4766 4862
Size: 30 165 30 2630 30 673 30 96
Cuts at: 4862 4892 6647 6677 7286 7316 7973 8003 8699
Size: 30 1755 30 609 30 657 30 696
Cuts at: 8699 8729 8915 8945 9209 9239 9580 9610 10301
Size: 30 186 30 264 30 341 30 691
Cuts at: 10301 10331 12439 12469 12527 12557 14520 14550 14658
Size: 30 2108 30 58 30 1963 30 108
Cuts at: 14658 14688 14850 14880 16679 16709 1178
Size: 30 162 30 1799 30 5782
Fragments arranged by size:
5782 2630 2108 1963 1799 1755 696 691
673 657 609 341 264 186 165 162
108 96 58 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2157 2693 3326 3353 3498 3515 3566 3749
Size: 971 536 633 27 145 17 51 183
Cuts at: 3749 3813 3857 4046 4181 4208 4262 4289 4316
Size: 64 44 189 135 27 54 27 27
Cuts at: 4316 4343 4370 4451 4478 4532 4625 4652 4757
Size: 27 27 81 27 54 93 27 105
Cuts at: 4757 4803 5058 5124 5492 5967 6068 6071 7052
Size: 46 255 66 368 475 101 3 981
Cuts at: 7052 8045 8923 9075 9144 11306 11384 12548 12602
Size: 993 878 152 69 2162 78 1164 54
Cuts at: 12602 13950 14048 14666 16166 16920 17126 17888 18533
Size: 1348 98 618 1500 754 206 762 645
Cuts at: 18533 1186
Size: 3966
Fragments arranged by size:
3966 2162 1500 1348 1164 993 981 971
878 762 754 645 633 618 536 475
368 255 206 189 183 152 145 135
105 101 98 93 81 78 69 66
64 54 54 54 51 46 44 27
27 27 27 27 27 27 27 17
3
BseYI C'CCAG_C
Cuts at: 2412 4320 5254 5321 5332 5828 5887 8016 8635
Size: 1908 934 67 11 496 59 2129 619
Cuts at: 8635 9417 9443 9466 9473 9484 9788 10158 10172
Size: 782 26 23 7 11 304 370 14
Cuts at: 10172 10220 10949 11594 11820 11921 11979 11997 12108
Size: 48 729 645 226 101 58 18 111
Cuts at: 12108 12428 12465 12552 13337 13405 13515 13566 14092
Size: 320 37 87 785 68 110 51 526
Cuts at: 14092 14241 14247 14258 14978 14985 15003 15090 15374
Size: 149 6 11 720 7 18 87 284
Cuts at: 15374 15488 15518 15825 15943 20516 21012 2412
Size: 114 30 307 118 4573 496 2713
Fragments arranged by size:
4573 2713 2129 1908 934 785 782 729
720 645 619 526 496 496 370 320
307 304 284 226 149 118 114 111
110 101 87 87 68 67 59 58
51 48 37 30 26 23 18 18
14 11 11 11 7 7 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 2712 3579 4427 4614 4957 5198
Size: 573 776 200 867 848 187 343 241
Cuts at: 5198 6809 7131 8249 9843 11847 15923 17237 17510
Size: 1611 322 1118 1594 2004 4076 1314 273
Cuts at: 17510 1163
Size: 4966
Fragments arranged by size:
4966 4076 2004 1611 1594 1314 1118 867
848 776 573 343 322 273 241 200
187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3467 8300 17717 18763 19524 20552
Size: 275 186 1466 4833 9417 1046 761 1028
Cuts at: 20552 1540
Size: 2301
Fragments arranged by size:
9417 4833 2301 1466 1046 1028 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2206 2655 5132 5466 5575 6203 6978 8067 8478
Size: 449 2477 334 109 628 775 1089 411
Cuts at: 8478 9193 9519 10408 10913 11208 11346 11646 11717
Size: 715 326 889 505 295 138 300 71
Cuts at: 11717 12119 12765 13506 14599 15605 15954 17178 17279
Size: 402 646 741 1093 1006 349 1224 101
Cuts at: 17279 17444 17880 18101 18147 19374 19459 20530 2206
Size: 165 436 221 46 1227 85 1071 2989
Fragments arranged by size:
2989 2477 1227 1224 1093 1089 1071 1006
889 775 741 715 646 628 505 449
436 411 402 349 334 326 300 295
221 165 138 109 101 85 71 46
BsiWI C'GTAC_G
Cuts at: 7754 7754
Size: 21313
BsmI GAATG_Cn'
Cuts at: 2825 5092 5255 5522 8316 11848 18824 18917 2825
Size: 2267 163 267 2794 3532 6976 93 5221
Fragments arranged by size:
6976 5221 3532 2794 2267 267 163 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2873 3596 11127 2873
Size: 723 7531 13059
Fragments arranged by size:
13059 7531 723
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2206 2226 2312 2319 2419 2468 2655
Size: 399 416 20 86 7 100 49 187
Cuts at: 2655 2816 2993 3159 3204 3237 3288 5061 5132
Size: 161 177 166 45 33 51 1773 71
Cuts at: 5132 5466 5543 5575 5679 5690 5771 5959 5986
Size: 334 77 32 104 11 81 188 27
Cuts at: 5986 6203 6501 6723 6747 6894 6978 7007 7572
Size: 217 298 222 24 147 84 29 565
Cuts at: 7572 7703 7908 7928 8067 8338 8447 8478 8505
Size: 131 205 20 139 271 109 31 27
Cuts at: 8505 8562 8617 8783 8814 8904 9021 9029 9193
Size: 57 55 166 31 90 117 8 164
Cuts at: 9193 9519 9590 9705 9789 10173 10408 10679 10700
Size: 326 71 115 84 384 235 271 21
Cuts at: 10700 10913 11208 11288 11346 11430 11510 11535 11646
Size: 213 295 80 58 84 80 25 111
Cuts at: 11646 11717 11906 11962 12119 12152 12765 13395 13412
Size: 71 189 56 157 33 613 630 17
Cuts at: 13412 13460 13469 13506 13688 14044 14124 14394 14465
Size: 48 9 37 182 356 80 270 71
Cuts at: 14465 14599 14858 15277 15286 15328 15471 15605 15954
Size: 134 259 419 9 42 143 134 349
Cuts at: 15954 15994 16181 16210 16282 16469 16550 16652 16797
Size: 40 187 29 72 187 81 102 145
Cuts at: 16797 16897 17178 17191 17230 17279 17424 17444 17880
Size: 100 281 13 39 49 145 20 436
Cuts at: 17880 18019 18101 18147 18234 18436 18632 19374 19459
Size: 139 82 46 87 202 196 742 85
Cuts at: 19459 20530 1391
Size: 1071 2174
Fragments arranged by size:
2174 1773 1071 742 630 613 565 436
419 416 399 384 356 349 334 326
298 295 281 271 271 270 259 235
222 217 213 205 202 196 189 188
187 187 187 182 177 166 166 164
161 157 147 145 145 143 139 139
134 134 131 117 115 111 109 104
102 100 100 90 87 86 85 84
84 84 82 81 81 80 80 80
77 72 71 71 71 71 58 57
56 55 51 49 49 48 46 45
42 40 39 37 33 33 32 31
31 29 29 27 27 25 24 21
20 20 20 17 13 11 9 9
8 7
BspEI T'CCGG_A
Cuts at: 12744 20976 12744
Size: 8232 13081
BspHI T'CATG_A
Cuts at: 8136 17107 17994 19202 20932 8136
Size: 8971 887 1208 1730 8517
Fragments arranged by size:
8971 8517 1730 1208 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 2109 2389 4129 6527 7204 7212 7680
Size: 1227 38 280 1740 2398 677 8 468
Cuts at: 7680 8190 9500 9865 11130 11154 11443 11592 11686
Size: 510 1310 365 1265 24 289 149 94
Cuts at: 11686 11945 14324 14406 15088 16035 18668 844
Size: 259 2379 82 682 947 2633 3489
Fragments arranged by size:
3489 2633 2398 2379 1740 1310 1265 1227
947 682 677 510 468 365 289 280
259 149 94 82 38 24 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2559 3081 12445 14485 17020 17476 19200
Size: 429 736 522 9364 2040 2535 456 1724
Cuts at: 19200 1394
Size: 3507
Fragments arranged by size:
9364 3507 2535 2040 1724 736 522 456
429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3248 3449 9395 11406 12198 12270 19808
Size: 1183 2000 201 5946 2011 792 72 7538
Cuts at: 19808 19982 65
Size: 174 1396
Fragments arranged by size:
7538 5946 2011 2000 1396 1183 792 201
174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 4849 5035 5575 5940 7386 8299
Size: 110 237 2802 186 540 365 1446 913
Cuts at: 8299 8399 8437 10365 10813 10896 16185 18165 18312
Size: 100 38 1928 448 83 5289 1980 147
Cuts at: 18312 18398 18498 19957 20144 1700
Size: 86 100 1459 187 2869
Fragments arranged by size:
5289 2869 2802 1980 1928 1459 1446 913
540 448 365 237 187 186 147 110
100 100 86 83 38
BsrGI T'GTAC_A
Cuts at: 96 10651 12601 96
Size: 10555 1950 8808
Fragments arranged by size:
10555 8808 1950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 21313
BssSI C'ACGA_G
Cuts at: 5216 5946 19373 20385 5216
Size: 730 13427 1012 6144
Fragments arranged by size:
13427 6144 1012 730
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2829 2961 10130 11438 13183 13717 14554 15747
Size: 2035 132 7169 1308 1745 534 837 1193
Cuts at: 15747 17325 17902 18142 794
Size: 1578 577 240 3965
Fragments arranged by size:
7169 3965 2035 1745 1578 1308 1193 837
577 534 240 132
BstBI TT'CG_AA
Cuts at: 6762 13120 20117 6762
Size: 6358 6997 7958
Fragments arranged by size:
7958 6997 6358
BstEII G'GTnAC_C
Cuts at: 1184 6514 1184
Size: 5330 15983
BstXI CCAn_nnnn'nTGG
Cuts at: 2274 5053 5728 8951 10158 10936 12046 12289 14738
Size: 2779 675 3223 1207 778 1110 243 2449
Cuts at: 14738 15209 17294 2274
Size: 471 2085 6293
Fragments arranged by size:
6293 3223 2779 2449 2085 1207 1110 778
675 471 243
BstYI r'GATC_y
Cuts at: 1972 2505 4801 5625 9869 10083 10969 11618 12720
Size: 533 2296 824 4244 214 886 649 1102
Cuts at: 12720 13070 15133 16431 18567 18643 19010 19395 19412
Size: 350 2063 1298 2136 76 367 385 17
Cuts at: 19412 20864 20950 20962 21308 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
4244 2296 2136 2063 1977 1452 1298 1102
886 824 649 533 385 367 350 346
214 86 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2169 2367 3336 3363 3390 3417 3444 3498 3678
Size: 198 969 27 27 27 27 54 180
Cuts at: 3678 3732 3840 3867 3894 3948 4002 4029 4056
Size: 54 108 27 27 54 54 27 27
Cuts at: 4056 4083 4110 4191 4245 4272 4326 4353 4407
Size: 27 27 81 54 27 54 27 54
Cuts at: 4407 4461 4488 4542 4608 4635 7539 8935 9041
Size: 54 27 54 66 27 2904 1396 106
Cuts at: 9041 9618 11210 11289 11349 14422 14850 15039 15225
Size: 577 1592 79 60 3073 428 189 186
Cuts at: 15225 2169
Size: 8257
Fragments arranged by size:
8257 3073 2904 1592 1396 969 577 428
198 189 186 180 108 106 81 79
66 60 54 54 54 54 54 54
54 54 54 27 27 27 27 27
27 27 27 27 27 27 27 27
27
BtgI C'CryG_G
Cuts at: 513 1065 2004 2573 2922 5721 5959 6090 7221
Size: 552 939 569 349 2799 238 131 1131
Cuts at: 7221 7504 7965 8571 9498 9679 9889 11562 12039
Size: 283 461 606 927 181 210 1673 477
Cuts at: 12039 12144 12916 13608 13680 14347 17199 17622 513
Size: 105 772 692 72 667 2852 423 4204
Fragments arranged by size:
4204 2852 2799 1673 1131 939 927 772
692 667 606 569 552 477 461 423
349 283 238 210 181 131 105 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3615 5838 7443 10320 487
Size: 683 56 2389 2223 1605 2877 11480
Fragments arranged by size:
11480 2877 2389 2223 1605 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 2334 2524 2630 2700 2775 2816 3320 5792
Size: 149 190 106 70 75 41 504 2472
Cuts at: 5792 6203 6380 6443 6821 7760 9206 10146 11533
Size: 411 177 63 378 939 1446 940 1387
Cuts at: 11533 12409 12642 14368 15748 16413 16593 17005 17607
Size: 876 233 1726 1380 665 180 412 602
Cuts at: 17607 17921 18829 19623 19643 2185
Size: 314 908 794 20 3855
Fragments arranged by size:
3855 2472 1726 1446 1387 1380 940 939
908 876 794 665 602 504 412 411
378 314 233 190 180 177 149 106
75 70 63 41 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 21313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 10252 10287 10433 10468 529
Size: 35 9688 35 146 35 11374
Fragments arranged by size:
11374 9688 146 35 35 35
DraI TTT'AAA
Cuts at: 13935 18617 13935
Size: 4682 16631
DraIII CAC_nnn'GTG
Cuts at: 5549 6494 11606 13652 5549
Size: 945 5112 2046 13210
Fragments arranged by size:
13210 5112 2046 945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6119 20320 809
Size: 507 4803 14201 1802
Fragments arranged by size:
14201 4803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3464 6160 6301
Size: 54 1425 50 275 18 1634 2696 141
Cuts at: 6301 7388 8662 10322 10537 10921 11055 12436 12658
Size: 1087 1274 1660 215 384 134 1381 222
Cuts at: 12658 12913 14175 14616 17714 18306 18447 18760 19649
Size: 255 1262 441 3098 592 141 313 889
Cuts at: 19649 21278 8
Size: 1629 43
Fragments arranged by size:
3098 2696 1660 1634 1629 1425 1381 1274
1262 1087 889 592 441 384 313 275
255 222 215 141 141 134 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3464 17714 18760 1537
Size: 275 1652 14250 1046 4090
Fragments arranged by size:
14250 4090 1652 1046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3570 3756 3810 5556 6104 6543 6617 7277
Size: 2217 186 54 1746 548 439 74 660
Cuts at: 7277 7688 8483 8865 11016 11911 13122 14022 15258
Size: 411 795 382 2151 895 1211 900 1236
Cuts at: 15258 15290 15933 16010 16604 17855 18327 19243 1353
Size: 32 643 77 594 1251 472 916 3423
Fragments arranged by size:
3423 2217 2151 1746 1251 1236 1211 916
900 895 795 660 643 594 548 472
439 411 382 186 77 74 54 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3480 7169 9159 16427 19900 20274
Size: 1064 138 1891 3689 1990 7268 3473 374
Cuts at: 20274 20420 387
Size: 146 1280
Fragments arranged by size:
7268 3689 3473 1990 1891 1280 1064 374
146 138
EcoICRI GAG'CTC
Cuts at: 8476 9517 11644 11715 12117 15603 17442 17878 8476
Size: 1041 2127 71 402 3486 1839 436 11911
Fragments arranged by size:
11911 3486 2127 1839 1041 436 402 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2421 2696 3491 3569 3645 3726
Size: 393 509 280 275 795 78 76 81
Cuts at: 3726 3780 3942 4184 4958 5001 5072 5676 6693
Size: 54 162 242 774 43 71 604 1017
Cuts at: 6693 7019 7260 7313 7795 7994 8167 8450 8576
Size: 326 241 53 482 199 173 283 126
Cuts at: 8576 8978 9318 9447 10052 10134 10776 11114 12002
Size: 402 340 129 605 82 642 338 888
Cuts at: 12002 12596 13071 13305 13467 13613 13685 13721 13763
Size: 594 475 234 162 146 72 36 42
Cuts at: 13763 14058 14088 14522 14744 14807 15515 15957 15990
Size: 295 30 434 222 63 708 442 33
Cuts at: 15990 16310 16592 16628 16755 16926 17771 17885 18478
Size: 320 282 36 127 171 845 114 593
Cuts at: 18478 19376 19973 20760 21028 1239
Size: 898 597 787 268 1524
Fragments arranged by size:
1524 1017 898 888 845 795 787 774
708 642 605 604 597 594 593 509
482 475 442 434 402 393 340 338
326 320 295 283 282 280 275 268
242 241 234 222 199 173 171 162
162 146 129 127 126 114 82 81
78 76 72 71 63 54 53 43
42 36 36 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3308 5949 6128 8186 8787 10103 16640 16908 3308
Size: 2641 179 2058 601 1316 6537 268 7713
Fragments arranged by size:
7713 6537 2641 2058 1316 601 268 179
EcoO109I rG'GnC_Cy
Cuts at: 2077 2315 2344 2371 2406 2464 2763 3233 3234
Size: 238 29 27 35 58 299 470 1
Cuts at: 3234 3298 5302 6498 6553 6931 7003 7004 7139
Size: 64 2004 1196 55 378 72 1 135
Cuts at: 7139 7363 7407 7535 7669 7770 8524 8613 8614
Size: 224 44 128 134 101 754 89 1
Cuts at: 8614 8851 8901 9045 9529 9785 9997 10145 10293
Size: 237 50 144 484 256 212 148 148
Cuts at: 10293 10419 10589 10667 10696 10735 10768 10862 11005
Size: 126 170 78 29 39 33 94 143
Cuts at: 11005 11251 11276 11285 11752 11870 11925 12395 12938
Size: 246 25 9 467 118 55 470 543
Cuts at: 12938 13280 13310 13324 13391 13392 13409 13457 13465
Size: 342 30 14 67 1 17 48 8
Cuts at: 13465 13466 13537 14120 14121 14462 14528 14742 14764
Size: 1 71 583 1 341 66 214 22
Cuts at: 14764 14816 14840 14854 15027 15125 15359 15780 16156
Size: 52 24 14 173 98 234 421 376
Cuts at: 16156 16207 16278 16279 16294 16511 17420 17421 17545
Size: 51 71 1 15 217 909 1 124
Cuts at: 17545 17746 17755 18223 18247 2077
Size: 201 9 468 24 5143
Fragments arranged by size:
5143 2004 1196 909 754 583 543 484
470 470 468 467 421 378 376 342
341 299 256 246 238 237 234 224
217 214 212 201 173 170 148 148
144 143 135 134 128 126 124 118
101 98 94 89 78 72 71 71
67 66 64 58 55 55 52 51
50 48 44 39 35 33 30 29
29 27 25 24 24 22 17 15
14 14 9 9 8 1 1 1
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 8675 18622 19000 8675
Size: 9947 378 10988
Fragments arranged by size:
10988 9947 378
EcoRV GAT'ATC
Cuts at: 11194 12259 11194
Size: 1065 20248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 7628 11212 11569 14006 15567 2512
Size: 5116 3584 357 2437 1561 8258
Fragments arranged by size:
8258 5116 3584 2437 1561 357
FseI GG_CCGG'CC
Cuts at: 7390 7390
Size: 21313
FspI TGC'GCA
Cuts at: 8622 18176 19819 8622
Size: 9554 1643 10116
Fragments arranged by size:
10116 9554 1643
HaeII r_GCGC'y
Cuts at: 1678 2257 2518 6698 6838 7221 8017 8643 10051
Size: 579 261 4180 140 383 796 626 1408
Cuts at: 10051 11299 13674 15800 17971 20460 1678
Size: 1248 2375 2126 2171 2489 2531
Fragments arranged by size:
4180 2531 2489 2375 2171 2126 1408 1248
796 626 579 383 261 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 4898 4930 5333
Size: 32 135 32 761 32 2533 32 403
Cuts at: 5333 5365 5377 5409 5617 5649 5753 5785 6647
Size: 32 12 32 208 32 104 32 862
Cuts at: 6647 6679 7418 7450 7484 7516 7973 8005 8915
Size: 32 739 32 34 32 457 32 910
Cuts at: 8915 8947 9209 9241 9897 9929 10301 10333 14369
Size: 32 262 32 656 32 372 32 4036
Cuts at: 14369 14401 14520 14552 15444 15476 15953 15985 19954
Size: 32 119 32 892 32 477 32 3969
Cuts at: 19954 19986 20028 20060 1373
Size: 32 42 32 2626
Fragments arranged by size:
4036 3969 2626 2533 910 892 862 761
739 656 477 457 403 372 262 208
135 119 104 42 34 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5620 5997 6229 8923 10894 18396
Size: 988 281 3682 377 232 2694 1971 7502
Cuts at: 18396 18657 18903 669
Size: 261 246 3079
Fragments arranged by size:
7502 3682 3079 2694 1971 988 377 281
261 246 232
HindIII A'AGCT_T
Cuts at: 748 7393 8802 9300 10389 748
Size: 6645 1409 498 1089 11672
Fragments arranged by size:
11672 6645 1409 1089 498
HpaI GTT'AAC
Cuts at: 5620 18903 5620
Size: 13283 8030
KasI G'GCGC_C
Cuts at: 6694 6834 7217 8639 17967 6694
Size: 140 383 1422 9328 10040
Fragments arranged by size:
10040 9328 1422 383 140
KpnI G_GTAC'C
Cuts at: 9993 12107 15188 15516 17078 18638 9993
Size: 2114 3081 328 1562 1560 12668
Fragments arranged by size:
12668 3081 2114 1562 1560 328
MfeI C'AATT_G
Cuts at: 12185 18912 12185
Size: 6727 14586
MluI A'CGCG_T
Cuts at: 21189 21189
Size: 21313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3273 3301 5634 6129 6336 9240 10868 11739
Size: 1968 28 2333 495 207 2904 1628 871
Cuts at: 11739 14075 14260 14545 18578 20427 20611 1305
Size: 2336 185 285 4033 1849 184 2007
Fragments arranged by size:
4033 2904 2336 2333 2007 1968 1849 1628
871 495 285 207 185 184 28
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6303 8664 11057 12438 12915
Size: 54 1425 343 4471 2361 2393 1381 477
Cuts at: 12915 14177 14618 18308 10
Size: 1262 441 3690 3015
Fragments arranged by size:
4471 3690 3015 2393 2361 1425 1381 1262
477 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2566 4339 5051 5818 6140 6245
Size: 648 1261 139 1773 712 767 322 105
Cuts at: 6245 6728 8949 11115 12044 12170 12335 13059 17204
Size: 483 2221 2166 929 126 165 724 4145
Cuts at: 17204 18077 18353 18429 19271 19630 19789 518
Size: 873 276 76 842 359 159 2042
Fragments arranged by size:
4145 2221 2166 2042 1773 1261 929 873
842 767 724 712 648 483 359 322
276 165 159 139 126 105 76
MspA1I CmG'CkG
Cuts at: 1606 2054 2242 2607 2638 2742 3048 3469 4162
Size: 448 188 365 31 104 306 421 693
Cuts at: 4162 4789 4838 5844 7111 7267 8002 8044 8165
Size: 627 49 1006 1267 156 735 42 121
Cuts at: 8165 8357 8745 9500 9795 9977 10121 10130 10158
Size: 192 388 755 295 182 144 9 28
Cuts at: 10158 10195 10616 11540 12205 12428 12523 12574 12845
Size: 37 421 924 665 223 95 51 271
Cuts at: 12845 12904 13162 13168 13189 13369 13519 13717 14272
Size: 59 258 6 21 180 150 198 555
Cuts at: 14272 14632 14686 14692 15664 15772 15829 17310 17512
Size: 360 54 6 972 108 57 1481 202
Cuts at: 17512 17572 18729 19406 20554 20799 1606
Size: 60 1157 677 1148 245 2120
Fragments arranged by size:
2120 1481 1267 1157 1148 1006 972 924
755 735 693 677 665 627 555 448
421 421 388 365 360 306 295 271
258 245 223 202 198 192 188 182
180 156 150 144 121 108 104 95
60 59 57 54 51 49 42 37
31 28 21 9 6 6
NaeI GCC'GGC
Cuts at: 5037 5942 7388 8401 8439 10367 18500 5037
Size: 905 1446 1013 38 1928 8133 7850
Fragments arranged by size:
8133 7850 1928 1446 1013 905 38
NarI GG'CG_CC
Cuts at: 6695 6835 7218 8640 17968 6695
Size: 140 383 1422 9328 10040
Fragments arranged by size:
10040 9328 1422 383 140
NcoI C'CATG_G
Cuts at: 513 1065 2004 2922 5721 5959 9679 9889 12039
Size: 552 939 918 2799 238 3720 210 2150
Cuts at: 12039 12144 12916 13608 13680 14347 513
Size: 105 772 692 72 667 7479
Fragments arranged by size:
7479 3720 2799 2150 939 918 772 692
667 552 238 210 105 72
NdeI CA'TA_TG
Cuts at: 387 9133 12066 387
Size: 8746 2933 9634
Fragments arranged by size:
9634 8746 2933
NgoMIV G'CCGG_C
Cuts at: 5035 5940 7386 8399 8437 10365 18498 5035
Size: 905 1446 1013 38 1928 8133 7850
Fragments arranged by size:
8133 7850 1928 1446 1013 905 38
NheI G'CTAG_C
Cuts at: 1052 12373 17498 1052
Size: 11321 5125 4867
Fragments arranged by size:
11321 5125 4867
NotI GC'GGCC_GC
Cuts at: 18760 18760
Size: 21313
NsiI A_TGCA'T
Cuts at: 6594 17409 6594
Size: 10815 10498
NspI r_CATG'y
Cuts at: 1132 1229 2117 3180 6139 6596 7303 10009 13405
Size: 97 888 1063 2959 457 707 2706 3396
Cuts at: 13405 14005 17089 17323 17374 17533 17842 17900 18082
Size: 600 3084 234 51 159 309 58 182
Cuts at: 18082 18358 18380 18671 20216 1132
Size: 276 22 291 1545 2229
Fragments arranged by size:
3396 3084 2959 2706 2229 1545 1063 888
707 600 457 309 291 276 234 182
159 97 58 51 22
PasI CC'CwG_GG
Cuts at: 1348 2300 2312 3061 4661 8783 9566 9781 9944
Size: 952 12 749 1600 4122 783 215 163
Cuts at: 9944 11944 12977 13321 13387 13396 13462 14044 15277
Size: 2000 1033 344 66 9 66 582 1233
Cuts at: 15277 17218 1348
Size: 1941 5443
Fragments arranged by size:
5443 4122 2000 1941 1600 1233 1033 952
783 749 582 344 215 163 66 66
12 9
PciI A'CATG_T
Cuts at: 2113 6135 7299 17529 18354 18376 20212 2113
Size: 4022 1164 10230 825 22 1836 3214
Fragments arranged by size:
10230 4022 3214 1836 1164 825 22
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 3134 3197 5414 7397 8834 9073 9680
Size: 912 1114 63 2217 1983 1437 239 607
Cuts at: 9680 9950 10592 10607 10747 11606 13306 14401 14906
Size: 270 642 15 140 859 1700 1095 505
Cuts at: 14906 15758 16556 18226 1108
Size: 852 798 1670 4195
Fragments arranged by size:
4195 2217 1983 1700 1670 1437 1114 1095
912 859 852 798 642 607 505 270
239 140 63 15
PfoI T'CCnGG_A
Cuts at: 4935 5466 7857 8843 10522 12614 13282 14293 14823
Size: 531 2391 986 1679 2092 668 1011 530
Cuts at: 14823 4935
Size: 11425
Fragments arranged by size:
11425 2391 2092 1679 1011 986 668 531
530
PmeI GTTT'AAAC
Cuts at: 18617 18617
Size: 21313
PmlI CAC'GTG
Cuts at: 3205 5570 3205
Size: 2365 18948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7286 7318 12527 12559 16439 16471 19382 19414 20895
Size: 32 5209 32 3880 32 2911 32 1481
Cuts at: 20895 20927 7286
Size: 32 7672
Fragments arranged by size:
7672 5209 3880 2911 1481 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2344 2406 5302 6553 6931 7407 7535 7669
Size: 267 62 2896 1251 378 476 128 134
Cuts at: 7669 8524 9529 9997 10293 10419 10667 10735 10862
Size: 855 1005 468 296 126 248 68 127
Cuts at: 10862 11276 11752 11925 12395 13280 13310 14528 14742
Size: 414 476 173 470 885 30 1218 214
Cuts at: 14742 15027 15125 16294 16511 17755 18247 2077
Size: 285 98 1169 217 1244 492 5143
Fragments arranged by size:
5143 2896 1251 1244 1218 1169 1005 885
855 492 476 476 470 468 414 378
296 285 267 248 217 214 173 134
128 127 126 98 68 62 30
PshAI GACnn'nnGTC
Cuts at: 1613 17088 1613
Size: 15475 5838
PsiI TTA'TAA
Cuts at: 18883 18883
Size: 21313
PspOMI G'GGCC_C
Cuts at: 2315 2464 3233 5982 6497 7003 8613 8900 9701
Size: 149 769 2749 515 506 1610 287 801
Cuts at: 9701 9785 10696 11284 13391 13408 13456 13465 14120
Size: 84 911 588 2107 17 48 9 655
Cuts at: 14120 14461 14854 16206 16278 17420 2315
Size: 341 393 1352 72 1142 6208
Fragments arranged by size:
6208 2749 2107 1610 1352 1142 911 801
769 655 588 515 506 393 341 287
149 84 72 48 17 9
PspXI vC'TCGA_Gb
Cuts at: 7511 7511
Size: 21313
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 18182 18214 18182
Size: 32 21281
PstI C_TGCA'G
Cuts at: 830 2068 2180 2637 2843 7213 7677 7767 10126
Size: 1238 112 457 206 4370 464 90 2359
Cuts at: 10126 10135 11683 11784 12253 12414 12927 13167 13173
Size: 9 1548 101 469 161 513 240 6
Cuts at: 13173 13206 14271 14277 14697 16378 18665 830
Size: 33 1065 6 420 1681 2287 3478
Fragments arranged by size:
4370 3478 2359 2287 1681 1548 1238 1065
513 469 464 457 420 240 206 161
112 101 90 33 9 6 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 21313
PvuII CAG'CTG
Cuts at: 2054 2638 2742 4789 4838 8044 8165 9977 10121
Size: 584 104 2047 49 3206 121 1812 144
Cuts at: 10121 10130 10158 10195 10616 11540 12205 12428 12574
Size: 9 28 37 421 924 665 223 146
Cuts at: 12574 12845 12904 13162 13168 13189 13369 13519 13717
Size: 271 59 258 6 21 180 150 198
Cuts at: 13717 14272 14632 14692 15664 15772 15829 17310 17572
Size: 555 360 60 972 108 57 1481 262
Cuts at: 17572 2054
Size: 5795
Fragments arranged by size:
5795 3206 2047 1812 1481 972 924 665
584 555 421 360 271 262 258 223
198 180 150 146 144 121 108 104
60 59 57 49 37 28 21 9
6
RsrII CG'GwC_CG
Cuts at: 4215 4215
Size: 21313
SacI G_AGCT'C
Cuts at: 8478 9519 11646 11717 12119 15605 17444 17880 8478
Size: 1041 2127 71 402 3486 1839 436 11911
Fragments arranged by size:
11911 3486 2127 1839 1041 436 402 71
SacII CC_GC'GG
Cuts at: 9501 9501
Size: 21313
SalI G'TCGA_C
Cuts at: 1936 18655 1936
Size: 16719 4594
SanDI GG'GwC_CC
Cuts at: 7407 10419 11925 13310 14742 15027 7407
Size: 3012 1506 1385 1432 285 13693
Fragments arranged by size:
13693 3012 1506 1432 1385 285
SapI GCTCTTCn'nnn_
Cuts at: 5556 7277 7688 8483 8865 11016 11911 13122 15258
Size: 1721 411 795 382 2151 895 1211 2136
Cuts at: 15258 15933 16604 18327 5556
Size: 675 671 1723 8542
Fragments arranged by size:
8542 2151 2136 1723 1721 1211 895 795
675 671 411 382
SbfI CC_TGCA'GG
Cuts at: 2843 18665 2843
Size: 15822 5491
ScaI AGT'ACT
Cuts at: 6752 19561 21273 6752
Size: 12809 1712 6792
Fragments arranged by size:
12809 6792 1712
SexAI A'CCwGG_T
Cuts at: 12343 15201 17757 12343
Size: 2858 2556 15899
Fragments arranged by size:
15899 2858 2556
SfcI C'TryA_G
Cuts at: 826 1045 2064 2176 2633 2839 7209 7673 7763
Size: 219 1019 112 457 206 4370 464 90
Cuts at: 7763 10122 10131 11679 11780 12249 12410 12563 12923
Size: 2359 9 1548 101 469 161 153 360
Cuts at: 12923 13163 13169 13202 14267 14273 14666 14693 14892
Size: 240 6 33 1065 6 393 27 199
Cuts at: 14892 15506 16374 18127 18661 19796 20477 20668 826
Size: 614 868 1753 534 1135 681 191 1471
Fragments arranged by size:
4370 2359 1753 1548 1471 1135 1065 1019
868 681 614 534 469 464 457 393
360 240 219 206 199 191 161 153
112 101 90 33 27 9 6 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 4850 13464 15647 4850
Size: 8614 2183 10516
Fragments arranged by size:
10516 8614 2183
SfoI GGC'GCC
Cuts at: 6696 6836 7219 8641 17969 6696
Size: 140 383 1422 9328 10040
Fragments arranged by size:
10040 9328 1422 383 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 21313
SgrAI Cr'CCGG_yG
Cuts at: 1700 5575 8437 1700
Size: 3875 2862 14576
Fragments arranged by size:
14576 3875 2862
SmaI CCC'GGG
Cuts at: 1872 9030 13499 15977 16475 17929 18640 1872
Size: 7158 4469 2478 498 1454 711 4545
Fragments arranged by size:
7158 4545 4469 2478 1454 711 498
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3281 3599 4568 6257 6953 7511
Size: 197 926 1338 318 969 1689 696 558
Cuts at: 7511 8093 8483 11645 12692 14641 14943 16627 16937
Size: 582 390 3162 1047 1949 302 1684 310
Cuts at: 16937 19417 20318 20580 820
Size: 2480 901 262 1553
Fragments arranged by size:
3162 2480 1949 1689 1684 1553 1338 1047
969 926 901 696 582 558 390 318
310 302 262 197
SnaBI TAC'GTA
Cuts at: 493 6544 19104 493
Size: 6051 12560 2702
Fragments arranged by size:
12560 6051 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 21313
SphI G_CATG'C
Cuts at: 1132 3180 6596 13405 14005 17323 17900 18671 1132
Size: 2048 3416 6809 600 3318 577 771 3774
Fragments arranged by size:
6809 3774 3416 3318 2048 771 600 577
SrfI GCCC'GGGC
Cuts at: 9030 16475 9030
Size: 7445 13868
SspI AAT'ATT
Cuts at: 5 52 19237 5
Size: 47 19185 2081
Fragments arranged by size:
19185 2081 47
StuI AGG'CCT
Cuts at: 1517 6308 7839 11549 13353 14381 15650 1517
Size: 4791 1531 3710 1804 1028 1269 7180
Fragments arranged by size:
7180 4791 3710 1804 1531 1269 1028
StyI C'CwwG_G
Cuts at: 513 1065 2004 2389 2922 3181 3222 5259 5356
Size: 552 939 385 533 259 41 2037 97
Cuts at: 5356 5721 5959 6243 6396 6904 7419 7665 8265
Size: 365 238 284 153 508 515 246 600
Cuts at: 8265 9525 9679 9714 9889 10549 10857 11058 11280
Size: 1260 154 35 175 660 308 201 222
Cuts at: 11280 11361 11418 11449 12039 12144 12916 13348 13608
Size: 81 57 31 590 105 772 432 260
Cuts at: 13608 13680 14347 14605 14905 15051 15128 15957 16282
Size: 72 667 258 300 146 77 829 325
Cuts at: 16282 16761 16797 16821 18243 18752 513
Size: 479 36 24 1422 509 3074
Fragments arranged by size:
3074 2037 1422 1260 939 829 772 667
660 600 590 552 533 515 509 508
479 432 385 365 325 308 300 284
260 259 258 246 238 222 201 175
154 153 146 105 97 81 77 72
57 41 36 35 31 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2935 3388 3757 3784 4840 4960 5608
Size: 1255 1355 453 369 27 1056 120 648
Cuts at: 5608 7058 8310 8939 8957 9111 9780 10326 11033
Size: 1450 1252 629 18 154 669 546 707
Cuts at: 11033 12011 15032 16681 17161 19340 19357 19510 19695
Size: 978 3021 1649 480 2179 17 153 185
Cuts at: 19695 20140 325
Size: 445 1498
Fragments arranged by size:
3021 2179 1649 1498 1450 1355 1255 1252
1056 978 707 669 648 629 546 480
453 445 369 185 154 153 120 27
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5783 6750 10651
Size: 274 80 33 51 1443 3806 967 3901
Cuts at: 10651 12601 13288 18276 19559 21271 96
Size: 1950 687 4988 1283 1712 138
Fragments arranged by size:
4988 3901 3806 1950 1712 1443 1283 967
687 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4033 4065 8699 8731 9580 9612 10669 10701 10846
Size: 32 4634 32 849 32 1057 32 145
Cuts at: 10846 10878 12439 12471 12596 12628 14850 14882 15204
Size: 32 1561 32 125 32 2222 32 322
Cuts at: 15204 15236 16069 16101 16329 16361 21273 21305 4033
Size: 32 833 32 228 32 4912 32 4041
Fragments arranged by size:
4912 4634 4041 2222 1561 1057 849 833
322 228 145 125 32 32 32 32
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6659 6814 13492 15632 17759 1316
Size: 5343 155 6678 2140 2127 4870
Fragments arranged by size:
6678 5343 4870 2140 2127 155
XbaI T'CTAG_A
Cuts at: 16021 18649 16021
Size: 2628 18685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2021 2113 5966 6016 7819 12425 14333 14786 16819
Size: 92 3853 50 1803 4606 1908 453 2033
Cuts at: 16819 16997 2021
Size: 178 6337
Fragments arranged by size:
6337 4606 3853 2033 1908 1803 453 178
92 50
XhoI C'TCGA_G
Cuts at: 1943 7511 11645 1943
Size: 5568 4134 11611
Fragments arranged by size:
11611 5568 4134
XmaI C'CCGG_G
Cuts at: 1870 9028 13497 15975 16473 17927 18638 1870
Size: 7158 4469 2478 498 1454 711 4545
Fragments arranged by size:
7158 4545 4469 2478 1454 711 498
XmnI GAAnn'nnTTC
Cuts at: 10761 19442 10761
Size: 8681 12632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 19121 276
Size: 53 83 186 936 17587 2468
Fragments arranged by size:
17587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BstZ17I FspAI NruI PacI SwaI