Restriction map for
pFN21AE9394
(Circular) (Six-base) MAPSORT of: pFN21AE9394.seq Check: 7784 from: 1 to: 8194
pFN21AE9394 8194 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6004 278
Size: 53 83 186 936 4468 2468
Fragments arranged by size:
4468 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3774 4824 5537 1937
Size: 1837 1050 713 4594
Fragments arranged by size:
4594 1837 1050 713
Acc65I G'GTAC_C
Cuts at: 4137 5515 4137
Size: 1378 6816
AclI AA'CG_TT
Cuts at: 1563 6321 6694 1563
Size: 4758 373 3063
Fragments arranged by size:
4758 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3459 3750 3770 5072 5090 5120 5232
Size: 1253 574 291 20 1302 18 30 112
Cuts at: 5232 6257 7641 7909 1632
Size: 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1253 1025 574 291 268
112 30 20 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7997
AflIII A'CryG_T
Cuts at: 1203 7093 8070 1203
Size: 5890 977 1327
Fragments arranged by size:
5890 1327 977
AgeI A'CCGG_T
Cuts at: 7025 7025
Size: 8194
AhdI GACnn_n'nnGTC
Cuts at: 2464 4509 6923 2464
Size: 2045 2414 3735
Fragments arranged by size:
3735 2414 2045
AleI CACnn'nnGTG
Cuts at: 1166 2460 1166
Size: 1294 6900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4434 1184
Size: 3250 4944
AlwNI CAG_nnn'CTG
Cuts at: 2933 3728 5458 5937 7509 2933
Size: 795 1730 479 1572 3618
Fragments arranged by size:
3618 1730 1572 795 479
ApaI G_GGCC'C
Cuts at: 5402 5402
Size: 8194
ApaLI G'TGCA_C
Cuts at: 6251 7407 6251
Size: 1156 7038
ApoI r'AATT_y
Cuts at: 1493 2064 2281 3035 4366 5104 5328 5369 5503
Size: 571 217 754 1331 738 224 41 134
Cuts at: 5503 5729 5881 7990 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 754 738 571 226 224
217 152 134 41
AseI AT'TA_AT
Cuts at: 160 1032 6748 160
Size: 872 5716 1606
Fragments arranged by size:
5716 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8194
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3389 4010 5519 1870
Size: 73 700 746 621 1509 4545
Fragments arranged by size:
4545 1509 746 700 621 73
BamHI G'GATC_C
Cuts at: 5524 5891 5524
Size: 367 7827
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3123 4137 4966 5515 6970
Size: 325 217 627 1336 1014 829 549 1455
Cuts at: 6970 618
Size: 1842
Fragments arranged by size:
1842 1455 1336 1014 829 627 549 325
217
BanII G_rGCy'C
Cuts at: 1391 5402 5513 1391
Size: 4011 111 4072
Fragments arranged by size:
4072 4011 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 2085 3180 3852 4509 4607 4619
Size: 971 123 63 1095 672 657 98 12
Cuts at: 4619 928
Size: 4503
Fragments arranged by size:
4503 1095 971 672 657 123 98 63
12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4475 4509 5450 5484 6385 6419 1903
Size: 34 2538 34 941 34 901 34 3678
Fragments arranged by size:
3678 2538 941 901 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6086 7296 6086
Size: 1210 6984
BclI T'GATC_A
Cuts at: 1548 2605 1548
Size: 1057 7137
BfrBI ATG'CAT
Cuts at: 2924 4796 4868 2924
Size: 1872 72 6250
Fragments arranged by size:
6250 1872 72
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6805 136
Size: 107 122 71 631 605 5133 1525
Fragments arranged by size:
5133 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2210 5431 8189 2210
Size: 3221 2758 2215
Fragments arranged by size:
3221 2758 2215
BlpI GC'TnA_GC
Cuts at: 5611 5611
Size: 8194
Bme1580I G_kGCm'C
Cuts at: 1790 3126 3536 5402 6255 7411 1790
Size: 1336 410 1866 853 1156 2573
Fragments arranged by size:
2573 1866 1336 1156 853 410
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2671 3546 5925
Size: 416 53 83 399 24 1254 875 2379
Cuts at: 5925 6883 442
Size: 958 1753
Fragments arranged by size:
2379 1753 1254 958 875 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3786 1056
Size: 2730 5464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3402 3999 4275 4507 3402
Size: 597 276 232 7089
Fragments arranged by size:
7089 597 276 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 3102 3259 3781 6854 1239
Size: 1077 786 157 522 3073 2579
Fragments arranged by size:
3073 2579 1077 786 522 157
Bpu10I CC'TnA_GC
Cuts at: 4884 4884
Size: 8194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3811 5106 6319 7184 7482 3486
Size: 325 1295 1213 865 298 4198
Fragments arranged by size:
4198 1295 1213 865 325 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2598 6857 882
Size: 617 1099 4259 2219
Fragments arranged by size:
4259 2219 1099 617
BsaAI yAC'GTr
Cuts at: 493 1204 5985 493
Size: 711 4781 2702
Fragments arranged by size:
4781 2702 711
BsaBI GATnn'nnATC
Cuts at: 8188 8188
Size: 8194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 6001 6383 7018
Size: 53 83 186 936 549 3919 382 635
Cuts at: 7018 275
Size: 1451
Fragments arranged by size:
3919 1451 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2019 6627 7025 7299 7446 7857 2019
Size: 4608 398 274 147 411 2356
Fragments arranged by size:
4608 2356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3842 3872 4565 4595 1178
Size: 30 165 30 2439 30 693 30 4777
Fragments arranged by size:
4777 2439 693 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2091 3229 4030 4651 1186
Size: 905 1138 801 621 4729
Fragments arranged by size:
4729 1138 905 801 621
BseYI C'CCAG_C
Cuts at: 2348 2870 4451 7397 7893 2348
Size: 522 1581 2946 496 2649
Fragments arranged by size:
2946 2649 1581 522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3639 4665 5444 1163
Size: 573 1903 1026 779 3913
Fragments arranged by size:
3913 1903 1026 779 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2461 5644 6405 7433 1540
Size: 275 186 460 3183 761 1028 2301
Fragments arranged by size:
3183 2301 1028 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2826 5097 6255 6340 7411 2826
Size: 2271 1158 85 1071 3609
Fragments arranged by size:
3609 2271 1158 1071 85
BsmI GAATG_Cn'
Cuts at: 2977 4870 4885 5705 5798 2977
Size: 1893 15 820 93 5373
Fragments arranged by size:
5373 1893 820 93 15
BsmBI CGTCTCn'nnnn_
Cuts at: 4547 4913 4547
Size: 366 7828
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2826 3126 3536 5097 5402 5513 6255
Size: 399 1036 300 410 1561 305 111 742
Cuts at: 6255 6340 7411 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1561 1071 1036 742 410 399 305
300 111 85
BspEI T'CCGG_A
Cuts at: 7857 7857
Size: 8194
BspHI T'CATG_A
Cuts at: 5424 6083 7813 5424
Size: 659 1730 5805
Fragments arranged by size:
5805 1730 659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2408 5549 844
Size: 1564 3141 3489
Fragments arranged by size:
3489 3141 1564
BsrBI CCG'CTC
Cuts at: 1394 1823 6081 1394
Size: 429 4258 3507
Fragments arranged by size:
4258 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6689 6863 65
Size: 1183 5441 174 1396
Fragments arranged by size:
5441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6838 7025 1700
Size: 110 5028 187 2869
Fragments arranged by size:
5028 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8194
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8194
BssSI C'ACGA_G
Cuts at: 3738 4249 6254 7266 3738
Size: 511 2005 1012 4666
Fragments arranged by size:
4666 2005 1012 511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4973 5458 794
Size: 4179 485 3530
Fragments arranged by size:
4179 3530 485
BstBI TT'CG_AA
Cuts at: 6998 6998
Size: 8194
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8194
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 2843
Size: 8194
BstYI r'GATC_y
Cuts at: 1972 2210 3302 3320 4471 4927 5268 5431 5524
Size: 238 1092 18 1151 456 341 163 93
Cuts at: 5524 5891 6276 6293 7745 7831 7843 8189 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1151 1092 456 385 367 346
341 238 163 93 86 18 17 12
BstZ17I GTA'TAC
Cuts at: 4825 4825
Size: 8194
Bsu36I CC'TnA_GG
Cuts at: 2377 5036 2377
Size: 2659 5535
BtgI C'CryG_G
Cuts at: 513 1065 2781 2799 2842 513
Size: 552 1716 18 43 5865
Fragments arranged by size:
5865 1716 552 43 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5029 5115 487
Size: 683 56 3803 86 3566
Fragments arranged by size:
3803 3566 683 86 56
BtsI GCAGTG_nn'
Cuts at: 2763 3175 5710 6504 6524 2763
Size: 412 2535 794 20 4433
Fragments arranged by size:
4433 2535 794 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2709 2744 529
Size: 35 2145 35 5979
Fragments arranged by size:
5979 2145 35 35
DraI TTT'AAA
Cuts at: 5498 5498
Size: 8194
DraIII CAC_nnn'GTG
Cuts at: 3662 3662
Size: 8194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7201 809
Size: 507 5885 1802
Fragments arranged by size:
5885 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5641 6530 8159
Size: 54 1425 50 275 18 3811 889 1629
Cuts at: 8159 8
Size: 43
Fragments arranged by size:
3811 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5641 1537
Size: 275 3829 4090
Fragments arranged by size:
4090 3829 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 3726 4089 4493 6124 1353
Size: 2125 248 363 404 1631 3423
Fragments arranged by size:
3423 2125 1631 404 363 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2068 6781 7155 7301 387
Size: 1064 138 479 4713 374 146 1280
Fragments arranged by size:
4713 1280 1064 479 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2885 3102 3259 3459 3750 3770
Size: 393 684 569 217 157 200 291 20
Cuts at: 3770 3781 5072 5090 5120 5232 6257 6854 7641
Size: 11 1291 18 30 112 1025 597 787
Cuts at: 7641 7909 1239
Size: 268 1524
Fragments arranged by size:
1524 1291 1025 787 684 597 569 393
291 268 217 200 157 112 30 20
18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3741 3741
Size: 8194
EcoO109I rG'GnC_Cy
Cuts at: 2339 2647 5399 2339
Size: 308 2752 5134
Fragments arranged by size:
5134 2752 308
EcoRI G'AATT_C
Cuts at: 4366 5104 5369 5503 5881 4366
Size: 738 265 134 378 6679
Fragments arranged by size:
6679 738 378 265 134
EcoRV GAT'ATC
Cuts at: 5490 5490
Size: 8194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3927 4741 4978 5262 3927
Size: 814 237 284 6859
Fragments arranged by size:
6859 814 284 237
FspI TGC'GCA
Cuts at: 6700 6700
Size: 8194
HaeII r_GCGC'y
Cuts at: 1678 7341 1678
Size: 5663 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2255 2287 2450
Size: 32 135 32 560 32 91 32 163
Cuts at: 2450 2482 3842 3874 3935 3967 4495 4527 4784
Size: 32 1360 32 61 32 528 32 257
Cuts at: 4784 4816 4912 4944 6835 6867 6909 6941 1373
Size: 32 96 32 1891 32 42 32 2626
Fragments arranged by size:
2626 1891 1360 560 528 257 163 135
96 91 61 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3900 5538 5784 669
Size: 988 281 1962 1638 246 3079
Fragments arranged by size:
3079 1962 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2222 4718 748
Size: 1474 2496 4224
Fragments arranged by size:
4224 2496 1474
HpaI GTT'AAC
Cuts at: 5784 5784
Size: 8194
KpnI G_GTAC'C
Cuts at: 4141 5519 4141
Size: 1378 6816
MfeI C'AATT_G
Cuts at: 5793 5793
Size: 8194
MluI A'CGCG_T
Cuts at: 8070 8070
Size: 8194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3071 4311 5331 7308 7492 1305
Size: 1766 1240 1020 1977 184 2007
Fragments arranged by size:
2007 1977 1766 1240 1020 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6372
Fragments arranged by size:
6372 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 2601 3255 4311 6152 6511 6670
Size: 648 1294 141 654 1056 1841 359 159
Cuts at: 6670 518
Size: 2042
Fragments arranged by size:
2042 1841 1294 1056 654 648 359 159
141
MspA1I CmG'CkG
Cuts at: 1606 2407 3725 4451 4982 5610 6287 7435 7680
Size: 801 1318 726 531 628 677 1148 245
Cuts at: 7680 1606
Size: 2120
Fragments arranged by size:
2120 1318 1148 801 726 677 628 531
245
NcoI C'CATG_G
Cuts at: 513 1065 2781 2799 2842 513
Size: 552 1716 18 43 5865
Fragments arranged by size:
5865 1716 552 43 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8194
NheI G'CTAG_C
Cuts at: 1052 3782 1052
Size: 2730 5464
NotI GC'GGCC_GC
Cuts at: 5641 5641
Size: 8194
NsiI A_TGCA'T
Cuts at: 2926 4798 4870 2926
Size: 1872 72 6250
Fragments arranged by size:
6250 1872 72
NspI r_CATG'y
Cuts at: 1132 1229 3793 5552 7097 1132
Size: 97 2564 1759 1545 2229
Fragments arranged by size:
2564 2229 1759 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8194
PciI A'CATG_T
Cuts at: 7093 7093
Size: 8194
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2917 5135 5278 1108
Size: 1809 2218 143 4024
Fragments arranged by size:
4024 2218 1809 143
PfoI T'CCnGG_A
Cuts at: 3589 3589
Size: 8194
PmeI GTTT'AAAC
Cuts at: 5498 5498
Size: 8194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6263 6295 7776 7808 6263
Size: 32 1481 32 6649
Fragments arranged by size:
6649 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2339 2647 2339
Size: 308 7886
PshAI GACnn'nnGTC
Cuts at: 1613 2794 1613
Size: 1181 7013
PsiI TTA'TAA
Cuts at: 5112 5764 5112
Size: 652 7542
PspOMI G'GGCC_C
Cuts at: 5398 5398
Size: 8194
PstI C_TGCA'G
Cuts at: 830 4987 5546 830
Size: 4157 559 3478
Fragments arranged by size:
4157 3478 559
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8194
PvuII CAG'CTG
Cuts at: 3725 4982 3725
Size: 1257 6937
SalI G'TCGA_C
Cuts at: 1936 5536 1936
Size: 3600 4594
SanDI GG'GwC_CC
Cuts at: 2647 2647
Size: 8194
SapI GCTCTTCn'nnn_
Cuts at: 3726 3726
Size: 8194
SbfI CC_TGCA'GG
Cuts at: 5546 5546
Size: 8194
ScaI AGT'ACT
Cuts at: 5004 5075 6442 8154 5004
Size: 71 1367 1712 5044
Fragments arranged by size:
5044 1712 1367 71
SfcI C'TryA_G
Cuts at: 826 1045 2244 2827 3687 3771 3873 4977 4983
Size: 219 1199 583 860 84 102 1104 6
Cuts at: 4983 5542 6677 7358 7549 826
Size: 559 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 1104 860 681 583 559
219 191 102 84 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8194
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8194
SmaI CCC'GGG
Cuts at: 1872 5521 1872
Size: 3649 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3501 3826 5121 6298 7199 7461
Size: 197 926 1558 325 1295 1177 901 262
Cuts at: 7461 820
Size: 1553
Fragments arranged by size:
1558 1553 1295 1177 926 901 325 262
197
SnaBI TAC'GTA
Cuts at: 493 5985 493
Size: 5492 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8194
SphI G_CATG'C
Cuts at: 1132 3793 5552 1132
Size: 2661 1759 3774
Fragments arranged by size:
3774 2661 1759
SspI AAT'ATT
Cuts at: 5 52 6118 5
Size: 47 6066 2081
Fragments arranged by size:
6066 2081 47
StuI AGG'CCT
Cuts at: 1517 3747 1517
Size: 2230 5964
StyI C'CwwG_G
Cuts at: 513 1065 2781 2799 2842 4215 4629 5633 513
Size: 552 1716 18 43 1373 414 1004 3074
Fragments arranged by size:
3074 1716 1373 1004 552 414 43 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2362 4619 6221 6238 6391 6576 7021
Size: 1255 782 2257 1602 17 153 185 445
Cuts at: 7021 325
Size: 1498
Fragments arranged by size:
2257 1602 1498 1255 782 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3218 5002 5073
Size: 274 80 33 51 1443 1241 1784 71
Cuts at: 5073 6440 8152 96
Size: 1367 1712 138
Fragments arranged by size:
1784 1712 1443 1367 1241 274 138 80
71 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5253 5285 8154 8186 5253
Size: 32 2869 32 5261
Fragments arranged by size:
5261 2869 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8194
XbaI T'CTAG_A
Cuts at: 5530 5530
Size: 8194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3414 3414
Size: 8194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8194
XmaI C'CCGG_G
Cuts at: 1870 5519 1870
Size: 3649 4545
XmnI GAAnn'nnTTC
Cuts at: 2195 4741 5209 6323 2195
Size: 2546 468 1114 4066
Fragments arranged by size:
4066 2546 1114 468
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6002 276
Size: 53 83 186 936 4468 2468
Fragments arranged by size:
4468 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI