Restriction map for
pFN21AE9288
(Circular) (Six-base) MAPSORT of: pFN21AE9288.seq Check: 602 from: 1 to: 6322
pFN21AE9288 6322 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2871 4132 278
Size: 53 83 186 936 1335 1261 2468
Fragments arranged by size:
2468 1335 1261 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3012 4385 5769 6037 1632
Size: 779 601 1373 1384 268 1917
Fragments arranged by size:
1917 1384 1373 779 601 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 3495 5221 6198 1203
Size: 2292 1726 977 1327
Fragments arranged by size:
2292 1726 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 2696 3107 3254 1166
Size: 1530 411 147 4234
Fragments arranged by size:
4234 1530 411 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2412 1184
Size: 1228 5094
AlwNI CAG_nnn'CTG
Cuts at: 2137 2365 4065 5637 2137
Size: 228 1700 1572 2822
Fragments arranged by size:
2822 1700 1572 228
ApaI G_GGCC'C
Cuts at: 2416 2736 2782 2416
Size: 320 46 5956
Fragments arranged by size:
5956 320 46
ApaLI G'TGCA_C
Cuts at: 3506 4379 5535 3506
Size: 873 1156 4293
Fragments arranged by size:
4293 1156 873
ApoI r'AATT_y
Cuts at: 1493 3003 3631 3857 4009 6118 1493
Size: 1510 628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1510 628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 3647 1870
Size: 73 98 1606 4545
Fragments arranged by size:
4545 1606 98 73
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2294 2581 3643 5098
Size: 325 217 627 284 223 287 1062 1455
Cuts at: 5098 618
Size: 1842
Fragments arranged by size:
1842 1455 1062 627 325 287 284 223
217
BanII G_rGCy'C
Cuts at: 1391 2416 2736 2782 3578 3641 1391
Size: 1025 320 46 796 63 4072
Fragments arranged by size:
4072 1025 796 320 63 46
BbeI G_GCGC'C
Cuts at: 2075 2585 2075
Size: 510 5812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2904 928
Size: 971 1005 4346
Fragments arranged by size:
4346 1005 971
BbvCI CC'TCA_GC
Cuts at: 2754 2754
Size: 6322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 4513 4547 1903
Size: 34 95 34 2447 34 3678
Fragments arranged by size:
3678 2447 95 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2500 4214 5424 2500
Size: 1714 1210 3398
Fragments arranged by size:
3398 1714 1210
BclI T'GATC_A
Cuts at: 1548 2343 1548
Size: 795 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 2370 4933
Size: 107 122 71 631 605 589 109 2563
Cuts at: 4933 136
Size: 1525
Fragments arranged by size:
2563 1525 631 605 589 122 109 107
71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2299 2416 2736 2782 3510 4383 5539 1790
Size: 509 117 320 46 728 873 1156 2573
Fragments arranged by size:
2573 1156 873 728 509 320 117 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 2353 2654
Size: 416 53 83 399 24 780 156 301
Cuts at: 2654 2902 4053 5011 442
Size: 248 1151 958 1753
Fragments arranged by size:
1753 1151 958 780 416 399 301 248
156 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2500 3566 4982 1239
Size: 858 403 1066 1416 2579
Fragments arranged by size:
2579 1416 1066 858 403
Bpu10I CC'TnA_GC
Cuts at: 2754 2754
Size: 6322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2893 3380 4447 5312 5610 2213
Size: 680 487 1067 865 298 2925
Fragments arranged by size:
2925 1067 865 680 487 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4985 882
Size: 617 1167 2319 2219
Fragments arranged by size:
2319 2219 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 3235 4113 493
Size: 711 2031 878 2702
Fragments arranged by size:
2702 2031 878 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2457 2582 2868
Size: 53 83 186 936 539 385 125 286
Cuts at: 2868 4129 4511 5146 275
Size: 1261 382 635 1451
Fragments arranged by size:
1451 1261 936 635 539 385 382 286
186 125 83 53
BsaWI w'CCGG_w
Cuts at: 4755 5153 5427 5574 5985 4755
Size: 398 274 147 411 5092
Fragments arranged by size:
5092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 2312 2342 1178
Size: 30 165 30 755 30 124 30 5158
Fragments arranged by size:
5158 755 165 124 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2333 3077 3173 1186
Size: 934 213 744 96 4335
Fragments arranged by size:
4335 934 744 213 96
BseYI C'CCAG_C
Cuts at: 2298 2595 2687 2703 5525 6021 2298
Size: 297 92 16 2822 496 2599
Fragments arranged by size:
2822 2599 496 297 92 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 3085 3397 1163
Size: 573 615 734 312 4088
Fragments arranged by size:
4088 734 615 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3108 3217 3772 4533 5561 1540
Size: 275 186 1107 109 555 761 1028 2301
Fragments arranged by size:
2301 1107 1028 761 555 275 186 109
BsiHKAI G_wGCw'C
Cuts at: 2118 3153 3510 4383 4468 5539 2118
Size: 1035 357 873 85 1071 2901
Fragments arranged by size:
2901 1071 1035 873 357 85
BsiWI C'GTAC_G
Cuts at: 2119 2119
Size: 6322
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
BsmBI CGTCTCn'nnnn_
Cuts at: 2570 2859 2570
Size: 289 6033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2299 2416 2736 2782 3153 3510
Size: 399 328 181 117 320 46 371 357
Cuts at: 3510 3578 3641 4383 4468 5539 1391
Size: 68 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 357 328 320
181 117 85 68 63 46
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 3207 4211 5941 3207
Size: 1004 1730 3588
Fragments arranged by size:
3588 1730 1004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3231 3677 844
Size: 2387 446 3489
Fragments arranged by size:
3489 2387 446
BsrBI CCG'CTC
Cuts at: 1394 1823 3172 4209 1394
Size: 429 1349 1037 3507
Fragments arranged by size:
3507 1349 1037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3459 4966 5153 1700
Size: 110 1649 1507 187 2869
Fragments arranged by size:
2869 1649 1507 187 110
BsrGI T'GTAC_A
Cuts at: 96 3490 96
Size: 3394 2928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 3155 4382 5394 3155
Size: 1227 1012 4083
Fragments arranged by size:
4083 1227 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 794
Size: 1399 4923
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 2338 3652 4019 4404 4421 5873 5959 5971
Size: 366 1314 367 385 17 1452 86 12
Cuts at: 5971 6317 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1314 385 367 366 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 2502 2564 2585 3501 513
Size: 552 939 22 476 62 21 916 3334
Fragments arranged by size:
3334 939 916 552 476 62 22 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 3359 3838 4632 4652 3359
Size: 479 794 20 5029
Fragments arranged by size:
5029 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2561 3105
Size: 54 1425 50 275 18 570 161 544
Cuts at: 3105 3457 3769 4658 6287 8
Size: 352 312 889 1629 43
Fragments arranged by size:
1629 1425 889 570 544 352 312 275
161 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3105 3769 1537
Size: 275 1293 664 4090
Fragments arranged by size:
4090 1293 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2957 4252 1353
Size: 1604 1295 3423
Fragments arranged by size:
3423 1604 1295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 3566 4909 5283 5429 387
Size: 1064 138 555 1422 1343 374 146 1280
Fragments arranged by size:
1422 1343 1280 1064 555 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2411 2500 3012 3566 4385 4982
Size: 393 465 314 89 512 554 819 597
Cuts at: 4982 5769 6037 1239
Size: 787 268 1524
Fragments arranged by size:
1524 819 787 597 554 512 465 393
314 268 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2858 2858
Size: 6322
EcoO109I rG'GnC_Cy
Cuts at: 2161 2412 2413 2655 2732 2778 2779 3020 3535
Size: 251 1 242 77 46 1 241 515
Cuts at: 3535 2161
Size: 4948
Fragments arranged by size:
4948 515 251 242 241 77 46 1
1
EcoRI G'AATT_C
Cuts at: 3003 3631 4009 3003
Size: 628 378 5316
Fragments arranged by size:
5316 628 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 6322
FspI TGC'GCA
Cuts at: 2054 4828 2054
Size: 2774 3548
HaeII r_GCGC'y
Cuts at: 1678 2075 2137 2585 3123 3232 5469 1678
Size: 397 62 448 538 109 2237 2531
Fragments arranged by size:
2531 2237 538 448 397 109 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2498 2530 4963
Size: 32 135 32 509 32 385 32 2433
Cuts at: 4963 4995 5037 5069 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2433 509 385 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2251 3666 3912 669
Size: 988 281 313 1415 246 3079
Fragments arranged by size:
3079 1415 988 313 281 246
HindIII A'AGCT_T
Cuts at: 748 3044 748
Size: 2296 4026
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2071 2581 2071
Size: 510 5812
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5436 5620 1305
Size: 4131 184 2007
Fragments arranged by size:
4131 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2563 10
Size: 54 1425 343 731 3769
Fragments arranged by size:
3769 1425 731 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 2696 3107 3254 3500 4280 4639
Size: 648 1228 302 411 147 246 780 359
Cuts at: 4639 4798 518
Size: 159 2042
Fragments arranged by size:
2042 1228 780 648 411 359 302 246
159 147
MspA1I CmG'CkG
Cuts at: 1606 2406 2520 3301 3385 3514 3738 4415 5563
Size: 800 114 781 84 129 224 677 1148
Cuts at: 5563 5808 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 800 781 677 245 224 129
114 84
NaeI GCC'GGC
Cuts at: 3461 3461
Size: 6322
NarI GG'CG_CC
Cuts at: 2072 2582 2072
Size: 510 5812
NcoI C'CATG_G
Cuts at: 513 1065 2004 2502 3501 513
Size: 552 939 498 999 3334
Fragments arranged by size:
3334 999 939 552 498
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 3459 3459
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2399 3499 3680 5225 1132
Size: 97 1170 1100 181 1545 2229
Fragments arranged by size:
2229 1545 1170 1100 181 97
PasI CC'CwG_GG
Cuts at: 1348 2615 2638 2783 1348
Size: 1267 23 145 4887
Fragments arranged by size:
4887 1267 145 23
PciI A'CATG_T
Cuts at: 3495 5221 3495
Size: 1726 4596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3563 1108
Size: 2455 3867
PfoI T'CCnGG_A
Cuts at: 3165 3165
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PmlI CAC'GTG
Cuts at: 3235 3235
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2655 3020 2161
Size: 494 365 5463
Fragments arranged by size:
5463 494 365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2412 2732 2778 2412
Size: 320 46 5956
Fragments arranged by size:
5956 320 46
PstI C_TGCA'G
Cuts at: 830 2244 3228 3674 830
Size: 1414 984 446 3478
Fragments arranged by size:
3478 1414 984 446
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 2406 2520 2406
Size: 114 6208
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SanDI GG'GwC_CC
Cuts at: 2655 3020 2655
Size: 365 5957
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 2450 4570 6282 2450
Size: 2120 1712 2490
Fragments arranged by size:
2490 2120 1712
SfcI C'TryA_G
Cuts at: 826 1045 2240 3224 3670 4805 5486 5677 826
Size: 219 1195 984 446 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 984 681 446 219 191
SfoI GGC'GCC
Cuts at: 2073 2583 2073
Size: 510 5812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 2228 2872 3395 4426 5327
Size: 197 926 98 187 644 523 1031 901
Cuts at: 5327 5589 820
Size: 262 1553
Fragments arranged by size:
1553 1031 926 901 644 523 262 197
187 98
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 1517
Size: 1011 5311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2502 3501 3761 513
Size: 552 939 498 999 260 3074
Fragments arranged by size:
3074 999 939 552 498 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 2783 4349 4366 4519 4704 5149
Size: 1255 794 409 1566 17 153 185 445
Cuts at: 5149 325
Size: 1498
Fragments arranged by size:
1566 1498 1255 794 445 409 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3490 4568
Size: 274 80 33 51 1443 471 1042 1078
Cuts at: 4568 6280 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1078 1042 471 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2158 2190 2266 2298 2312 2344 6282 6314 2158
Size: 32 76 32 14 32 3938 32 2166
Fragments arranged by size:
3938 2166 76 32 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 1316 2437 1316
Size: 1121 5201
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2695 2138
Size: 557 5765
XhoI C'TCGA_G
Cuts at: 1943 2041 1943
Size: 98 6224
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 4451 4451
Size: 6322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2869 4130 276
Size: 53 83 186 936 1335 1261 2468
Fragments arranged by size:
2468 1335 1261 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI