Restriction map for
pFN21AE9284
(Circular) (Six-base) MAPSORT of: pFN21AE9284.seq Check: 1663 from: 1 to: 5686
pFN21AE9284 5686 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2194 2416 2194
Size: 222 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2378 2604 3029 1937
Size: 441 226 425 4594
Fragments arranged by size:
4594 441 425 226
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 2760 3749 5133 5401 1632
Size: 848 280 989 1384 268 1917
Fragments arranged by size:
1917 1384 989 848 280 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2984 1184
Size: 1800 3886
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2321
Size: 32 5654
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 2169 3016 3383 2169
Size: 847 367 4472
Fragments arranged by size:
4472 847 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2882 3007 4462 618
Size: 325 217 627 1095 125 1455 1842
Fragments arranged by size:
1842 1455 1095 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 2218 2450 2868 2979 3005 1391
Size: 827 232 418 111 26 4072
Fragments arranged by size:
4072 827 418 232 111 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2845 1548
Size: 1297 4389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4297 136
Size: 107 122 71 631 605 337 2288 1525
Fragments arranged by size:
2288 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2488 5681 2488
Size: 3193 2493
BlpI GC'TnA_GC
Cuts at: 2556 2813 3103 2556
Size: 257 290 5139
Fragments arranged by size:
5139 290 257
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 2416 2860 1318
Size: 1098 444 4144
Fragments arranged by size:
4144 1098 444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 3417 4375
Size: 416 53 83 399 24 704 1296 958
Cuts at: 4375 442
Size: 1753
Fragments arranged by size:
1753 1296 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 4346 1239
Size: 1142 1965 2579
Fragments arranged by size:
2579 1965 1142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4349 882
Size: 617 2850 2219
Fragments arranged by size:
2850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2611 3477 493
Size: 711 1407 866 2702
Fragments arranged by size:
2702 1407 866 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2045 4119 4517 4791 4938 5349 2045
Size: 2074 398 274 147 411 2382
Fragments arranged by size:
2382 2074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2321 2348 2351 2378 1178
Size: 30 165 30 918 27 3 27 4486
Fragments arranged by size:
4486 918 165 30 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 1186
Size: 1159 4527
BseYI C'CCAG_C
Cuts at: 2087 4889 5385 2087
Size: 2802 496 2388
Fragments arranged by size:
2802 2388 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2681 1163
Size: 573 486 459 4168
Fragments arranged by size:
4168 573 486 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3136 3897 4925 1540
Size: 275 186 946 189 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 189 186
BsiHKAI G_wGCw'C
Cuts at: 2814 3747 3832 4903 2814
Size: 933 85 1071 3597
Fragments arranged by size:
3597 1071 933 85
BsmI GAATG_Cn'
Cuts at: 2973 3197 3290 2973
Size: 224 93 5369
Fragments arranged by size:
5369 224 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2450 2814 2868 2979 3005 3747
Size: 399 428 232 364 54 111 26 742
Cuts at: 3747 3832 4903 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 364 232 111
85 54 26
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 2764 3575 5305 2764
Size: 811 1730 3145
Fragments arranged by size:
3145 1730 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2416 3041 844
Size: 1350 222 625 3489
Fragments arranged by size:
3489 1350 625 222
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4181 4355 65
Size: 1183 2933 174 1396
Fragments arranged by size:
2933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2095
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2169 2488 2769 3016 3383 3768 3785 5237
Size: 197 319 281 247 367 385 17 1452
Cuts at: 5237 5323 5335 5681 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 319 281 247
197 86 17 12
BstZ17I GTA'TAC
Cuts at: 2379 2379
Size: 5686
BtgI C'CryG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 3916
Fragments arranged by size:
3916 939 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3202 3996 4016 2641
Size: 561 794 20 4311
Fragments arranged by size:
4311 794 561 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DraIII CAC_nnn'GTG
Cuts at: 2284 2284
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2414 4693 809
Size: 507 1098 2279 1802
Fragments arranged by size:
2279 1802 1098 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 3133 4022
Size: 54 1425 50 275 18 744 559 889
Cuts at: 4022 5651 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 744 559 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3616 1353
Size: 930 1333 3423
Fragments arranged by size:
3423 1333 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2480 2760 3749 4346 5133 5401
Size: 393 749 99 280 989 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 989 787 749 597 393 280 268
99
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 2777
Size: 5686
EcoO109I rG'GnC_Cy
Cuts at: 2752 2752
Size: 5686
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 2685 4833 1678
Size: 1007 2148 2531
Fragments arranged by size:
2531 2148 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2258 2290 2554
Size: 32 135 32 628 32 26 32 264
Cuts at: 2554 2586 2940 2972 4327 4359 4401 4433 1373
Size: 32 354 32 1355 32 42 32 2626
Fragments arranged by size:
2626 1355 628 354 264 135 42 32
32 32 32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 10
Size: 54 1425 343 744 3120
Fragments arranged by size:
3120 1425 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3644 4003 4162 518
Size: 648 1524 954 359 159 2042
Fragments arranged by size:
2042 1524 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 3102 3779 4927 5172 1606
Size: 452 1044 677 1148 245 2120
Fragments arranged by size:
2120 1148 1044 677 452 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4195
Fragments arranged by size:
4195 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2179 3044 4589 1132
Size: 97 950 865 1545 2229
Fragments arranged by size:
2229 1545 950 865 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 1108
Size: 1176 4510
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 3755 3787 5268 5300 2321
Size: 32 1402 32 1481 32 2707
Fragments arranged by size:
2707 1481 1402 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2752 2752
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2032 3038 830
Size: 1202 1006 3478
Fragments arranged by size:
3478 1202 1006
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2028 3034 4169 4850 5041 826
Size: 219 983 1006 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 983 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2179 3044 1132
Size: 1047 865 3774
Fragments arranged by size:
3774 1047 865
SspI AAT'ATT
Cuts at: 5 52 2315 3610 5
Size: 47 2263 1295 2081
Fragments arranged by size:
2263 2081 1295 47
StuI AGG'CCT
Cuts at: 1517 2735 2783 1517
Size: 1218 48 4420
Fragments arranged by size:
4420 1218 48
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 3125 513
Size: 552 939 321 800 3074
Fragments arranged by size:
3074 939 800 552 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2961 3713 3730 3883 4068 4513 325
Size: 1255 1381 752 17 153 185 445 1498
Fragments arranged by size:
1498 1381 1255 752 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2476 2508 5646 5678 2476
Size: 32 3138 32 2484
Fragments arranged by size:
3138 2484 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 2927 3815 2927
Size: 888 4798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI