Restriction map for
pFN21AE9281
(Circular) (Six-base) MAPSORT of: pFN21AE9281.seq Check: 9677 from: 1 to: 5332
pFN21AE9281 5332 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2197 2197
Size: 5332
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2378 2675 1937
Size: 441 297 4594
Fragments arranged by size:
4594 441 297
Acc65I G'GTAC_C
Cuts at: 2653 2653
Size: 5332
AclI AA'CG_TT
Cuts at: 1563 2631 3459 3832 1563
Size: 1068 828 373 3063
Fragments arranged by size:
3063 1068 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3395 4779 5047 1632
Size: 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 2558 3075 4647 2558
Size: 517 1572 3243
Fragments arranged by size:
3243 1572 517
ApaI G_GGCC'C
Cuts at: 2518 2518
Size: 5332
ApaLI G'TGCA_C
Cuts at: 3389 4545 3389
Size: 1156 4176
ApoI r'AATT_y
Cuts at: 1493 2206 2223 2641 2867 3019 5128 1493
Size: 713 17 418 226 152 2109 1697
Fragments arranged by size:
2109 1697 713 418 226 152 17
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2657 1870
Size: 73 135 579 4545
Fragments arranged by size:
4545 579 135 73
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2653 4108 618
Size: 325 217 627 866 1455 1842
Fragments arranged by size:
1842 1455 866 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2518 2651 1391
Size: 688 439 133 4072
Fragments arranged by size:
4072 688 439 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2384 2418 3523 3557 1903
Size: 34 50 34 363 34 1105 34 3678
Fragments arranged by size:
3678 1105 363 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3224 4434 3224
Size: 1210 4122
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3943 136
Size: 107 122 71 631 605 2271 1525
Fragments arranged by size:
2271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5327 5327
Size: 5332
BlpI GC'TnA_GC
Cuts at: 2749 2749
Size: 5332
Bme1580I G_kGCm'C
Cuts at: 1790 2518 3393 4549 1790
Size: 728 875 1156 2573
Fragments arranged by size:
2573 1156 875 728
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 3063 4021
Size: 416 53 83 399 24 685 961 958
Cuts at: 4021 442
Size: 1753
Fragments arranged by size:
1753 961 958 685 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2547 3992 1239
Size: 870 438 1445 2579
Fragments arranged by size:
2579 1445 870 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2355 3457 4322 4620 2355
Size: 1102 865 298 3067
Fragments arranged by size:
3067 1102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2371 3995 882
Size: 617 872 1624 2219
Fragments arranged by size:
2219 1624 872 617
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3139 3521 4156 275
Size: 53 83 186 936 1606 382 635 1451
Fragments arranged by size:
1606 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3765 4163 4437 4584 4995 3765
Size: 398 274 147 411 4102
Fragments arranged by size:
4102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5107
Fragments arranged by size:
5107 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5332
BseYI C'CCAG_C
Cuts at: 4535 5031 4535
Size: 496 4836
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4759
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3543 4571 1540
Size: 275 186 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 3393 3478 4549 2079
Size: 1314 85 1071 2862
Fragments arranged by size:
2862 1314 1071 85
BsmI GAATG_Cn'
Cuts at: 2843 2936 2843
Size: 93 5239
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 2341 2297
Size: 44 5288
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2518 2651 3393 3478 4549 1391
Size: 399 289 439 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 289 133 85
BspEI T'CCGG_A
Cuts at: 4995 4995
Size: 5332
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2197 2687 844
Size: 1353 490 3489
Fragments arranged by size:
3489 1353 490
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3976 4163 1700
Size: 110 2166 187 2869
Fragments arranged by size:
2869 2166 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5332
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2485 2011
Size: 474 4858
BstYI r'GATC_y
Cuts at: 1972 2268 2662 3029 3414 3431 4883 4969 4981
Size: 296 394 367 385 17 1452 86 12
Cuts at: 4981 5327 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 394 385 367 346 296 86
17 12
BstZ17I GTA'TAC
Cuts at: 2379 2379
Size: 5332
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4780
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2374 487
Size: 683 56 1148 3445
Fragments arranged by size:
3445 1148 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3642 3662 2848
Size: 794 20 4518
Fragments arranged by size:
4518 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4339 809
Size: 507 3023 1802
Fragments arranged by size:
3023 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2779 3668 5297
Size: 54 1425 50 275 18 949 889 1629
Cuts at: 5297 8
Size: 43
Fragments arranged by size:
1629 1425 949 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2779 1537
Size: 275 967 4090
Fragments arranged by size:
4090 967 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2424 3262 1353
Size: 1071 838 3423
Fragments arranged by size:
3423 1071 838
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 3919 4293 4439 387
Size: 1064 138 450 1880 374 146 1280
Fragments arranged by size:
1880 1280 1064 450 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2077
Size: 5332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2547 3395 3992 4779 5047 1239
Size: 393 477 438 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 477 438 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2083 2514 2515 2083
Size: 431 1 4900
Fragments arranged by size:
4900 431 1
EcoRI G'AATT_C
Cuts at: 2641 3019 2641
Size: 378 4954
EcoRV GAT'ATC
Cuts at: 2168 2168
Size: 5332
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 2034 4479 1678
Size: 356 2445 2531
Fragments arranged by size:
2531 2445 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2229 2261 3973 4005 4047
Size: 32 135 32 657 32 1712 32 42
Cuts at: 4047 4079 1373
Size: 32 2626
Fragments arranged by size:
2626 1712 657 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KpnI G_GTAC'C
Cuts at: 2657 2657
Size: 5332
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2395 4446 4630 1305
Size: 1090 2051 184 2007
Fragments arranged by size:
2051 2007 1090 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2483 3290 3649 3808 518
Size: 648 843 474 807 359 159 2042
Fragments arranged by size:
2042 843 807 648 474 359 159
MspA1I CmG'CkG
Cuts at: 1606 2748 3425 4573 4818 1606
Size: 1142 677 1148 245 2120
Fragments arranged by size:
2120 1148 1142 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4780
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5332
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 2264 1348
Size: 916 4416
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2114 1108
Size: 1006 4326
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2514 2514
Size: 5332
PspXI vC'TCGA_Gb
Cuts at: 2078 2078
Size: 5332
PstI C_TGCA'G
Cuts at: 830 2684 830
Size: 1854 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
SacI G_AGCT'C
Cuts at: 2079 2079
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2424 2680 3815 4496 4687 826
Size: 219 1379 256 1135 681 191 1471
Fragments arranged by size:
1471 1379 1135 681 256 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2370 3436 4337 4599 820
Size: 197 926 135 292 1066 901 262 1553
Fragments arranged by size:
1553 1066 926 901 292 262 197 135
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5332
StyI C'CwwG_G
Cuts at: 513 1065 2771 513
Size: 552 1706 3074
Fragments arranged by size:
3074 1706 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 3376 3529 3714 4159 325
Size: 1255 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3578 5290 96
Size: 274 80 33 51 1443 1601 1712 138
Fragments arranged by size:
1712 1601 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5292 5324 5292
Size: 32 5300
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5332
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 2482
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 2078 1943
Size: 135 5197
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI Bsu36I DraIII EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI