Restriction map for
pFN21AE9280
(Circular) (Six-base) MAPSORT of: pFN21AE9280.seq Check: 2257 from: 1 to: 5833
pFN21AE9280 5833 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2873 3643 278
Size: 53 83 186 936 1337 770 2468
Fragments arranged by size:
2468 1337 936 770 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2899 3896 5280 5548 1632
Size: 1267 997 1384 268 1917
Fragments arranged by size:
1917 1384 1267 997 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2988 3020 2552
Size: 32 404 32 5365
Fragments arranged by size:
5365 404 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2313 2449 2467 2953 3576 5148 2313
Size: 136 18 486 623 1572 2998
Fragments arranged by size:
2998 1572 623 486 136 18
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2387 3142 3368 3520 5629 1493
Size: 894 755 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 755 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2672 3158 1870
Size: 73 95 634 486 4545
Fragments arranged by size:
4545 634 486 95 73
BamHI G'GATC_C
Cuts at: 2436 3163 3530 2436
Size: 727 367 4739
Fragments arranged by size:
4739 727 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3119 3154 4609 618
Size: 325 217 627 1332 35 1455 1842
Fragments arranged by size:
1842 1455 1332 627 325 217 35
BanII G_rGCy'C
Cuts at: 1391 2249 2517 2673 3152 1391
Size: 858 268 156 479 4072
Fragments arranged by size:
4072 858 479 268 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BbvCI CC'TCA_GC
Cuts at: 2829 2829
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2741 2775 4024 4058 1903
Size: 34 804 34 1249 34 3678
Fragments arranged by size:
3678 1249 804 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2602 4444 136
Size: 107 122 71 631 605 930 1842 1525
Fragments arranged by size:
1842 1525 930 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2152 3124 3894 5050 1790
Size: 362 972 770 1156 2573
Fragments arranged by size:
2573 1156 972 770 362
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 2994 3564
Size: 416 53 83 399 24 1374 203 570
Cuts at: 3564 4522 442
Size: 958 1753
Fragments arranged by size:
1753 1374 958 570 416 399 203 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2471 2687 2930 2982 3005 4493 1239
Size: 917 315 216 243 52 23 1488 2579
Fragments arranged by size:
2579 1488 917 315 243 216 52 23
Bpu10I CC'TnA_GC
Cuts at: 2829 2829
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 3958 4823 5121 2102
Size: 1856 865 298 2814
Fragments arranged by size:
2814 1856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4496 882
Size: 617 1281 1716 2219
Fragments arranged by size:
2219 1716 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2870 3640 4022 4657
Size: 53 83 186 936 1337 770 382 635
Cuts at: 4657 275
Size: 1451
Fragments arranged by size:
1451 1337 936 770 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2195 2198 2369 2409 1186
Size: 922 87 3 171 40 4610
Fragments arranged by size:
4610 922 171 87 40 3
BseYI C'CCAG_C
Cuts at: 2901 5036 5532 2901
Size: 2135 496 3202
Fragments arranged by size:
3202 2135 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 3283 4044 5072 1540
Size: 275 186 29 1253 761 1028 2301
Fragments arranged by size:
2301 1253 1028 761 275 186 29
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2249 2517 2673 3124 3152 3894
Size: 399 362 97 268 156 451 28 742
Cuts at: 3894 3979 5050 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 362 268 156
97 85 28
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 2966 3720 1394
Size: 429 317 826 754 3507
Fragments arranged by size:
3507 826 754 429 317
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2294 4477 4664 1700
Size: 110 484 2183 187 2869
Fragments arranged by size:
2869 2183 484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2377
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2113 2436 2497 3163 3530 3915 3932 5384
Size: 141 323 61 666 367 385 17 1452
Cuts at: 5384 5470 5482 5828 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 666 385 367 346 323 141
86 61 17 12
Bsu36I CC'TnA_GG
Cuts at: 2468 2468
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2004 2209 2611 2731 513
Size: 552 939 205 402 120 3615
Fragments arranged by size:
3615 939 552 402 205 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2733 487
Size: 683 56 1507 3587
Fragments arranged by size:
3587 1507 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3020 4840 809
Size: 507 1704 1820 1802
Fragments arranged by size:
1820 1802 1704 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2050 2292
Size: 54 1425 50 275 18 197 23 242
Cuts at: 2292 2603 3037 3087 3280 4169 5798 8
Size: 311 434 50 193 889 1629 43
Fragments arranged by size:
1629 1425 889 434 311 275 242 197
193 54 50 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 3280 1537
Size: 275 215 1253 4090
Fragments arranged by size:
4090 1253 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 3763 1353
Size: 819 1591 3423
Fragments arranged by size:
3423 1591 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4420 4794 4940 387
Size: 1064 138 435 2396 374 146 1280
Fragments arranged by size:
2396 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2471 2687 2899 2930 2982 3005
Size: 393 524 315 216 212 31 52 23
Cuts at: 3005 3896 4493 5280 5548 1239
Size: 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 524 393 315 268
216 212 52 31 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2471 3110 2177
Size: 294 639 4900
Fragments arranged by size:
4900 639 294
EcoO109I rG'GnC_Cy
Cuts at: 2755 2833 2941 2755
Size: 78 108 5647
Fragments arranged by size:
5647 108 78
EcoRI G'AATT_C
Cuts at: 2387 3142 3520 2387
Size: 755 378 4700
Fragments arranged by size:
4700 755 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 5833
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2173 4980 1678
Size: 495 2807 2531
Fragments arranged by size:
2807 2531 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2565 2578 2597 4474
Size: 32 135 32 974 19 13 19 1877
Cuts at: 4474 4506 4548 4580 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1877 974 135 42 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 3177 3423 669
Size: 988 281 688 551 246 3079
Fragments arranged by size:
3079 988 688 551 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2821 4947 5131 1305
Size: 1516 2126 184 2007
Fragments arranged by size:
2126 2007 1516 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3039 10
Size: 54 1425 343 1207 2804
Fragments arranged by size:
2804 1425 1207 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3791 4150 4309 518
Size: 648 1419 1206 359 159 2042
Fragments arranged by size:
2042 1419 1206 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2192 2211 2428 3249 3926 5074 5319
Size: 540 46 19 217 821 677 1148 245
Cuts at: 5319 1606
Size: 2120
Fragments arranged by size:
2120 1148 821 677 540 245 217 46
19
NcoI C'CATG_G
Cuts at: 513 1065 2004 2731 513
Size: 552 939 727 3615
Fragments arranged by size:
3615 939 727 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2548 2924 3191 4736 1132
Size: 97 1319 376 267 1545 2229
Fragments arranged by size:
2229 1545 1319 376 267 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2953 1108
Size: 1845 3988
PfoI T'CCnGG_A
Cuts at: 2253 2253
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2755 2755
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2445 3099 3185 830
Size: 1615 654 86 3478
Fragments arranged by size:
3478 1615 654 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 5833
SacII CC_GC'GG
Cuts at: 2212 2212
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2441 2501 3095 3181 4316 4997 5188
Size: 219 1396 60 594 86 1135 681 191
Cuts at: 5188 826
Size: 1471
Fragments arranged by size:
1471 1396 1135 681 594 219 191 86
60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2602 2602
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 2040 2674 3160 1872
Size: 168 634 486 4545
Fragments arranged by size:
4545 634 486 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 3937 4838 5100 820
Size: 197 926 174 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197 174
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 2924 3191 1132
Size: 1792 267 3774
Fragments arranged by size:
3774 1792 267
SrfI GCCC'GGGC
Cuts at: 2674 2674
Size: 5833
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2731 3272 513
Size: 552 939 727 541 3074
Fragments arranged by size:
3074 939 727 552 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2727 3860 3877 4030 4215 4660 325
Size: 1255 1147 1133 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1147 1133 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2986 2326
Size: 660 5173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 2038 2672 3158 1870
Size: 168 634 486 4545
Fragments arranged by size:
4545 634 486 168
XmnI GAAnn'nnTTC
Cuts at: 2477 3962 2477
Size: 1485 4348
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2871 3641 276
Size: 53 83 186 936 1337 770 2468
Fragments arranged by size:
2468 1337 936 770 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfoI SwaI