Restriction map for
pFN21AE9279
(Circular) (Six-base) MAPSORT of: pFN21AE9279.seq Check: 1792 from: 1 to: 5575
pFN21AE9279 5575 bp
With 182 enzymes: SgfI *
March 29, 2013 17:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 2029
Size: 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2731 3318 4890 2731
Size: 587 1572 3416
Fragments arranged by size:
3416 1572 587
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2900 1870
Size: 73 131 826 4545
Fragments arranged by size:
4545 826 131 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 4351 618
Size: 325 217 627 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2894 1391
Size: 1503 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 928
Size: 971 631 3973
Fragments arranged by size:
3973 971 631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 3306 4264
Size: 416 53 83 399 24 1115 774 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1115 958 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4235 1239
Size: 2996 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2713 3366 493
Size: 711 1509 653 2702
Fragments arranged by size:
2702 1509 711 653
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2463 4008 4406 4680 4827 5238 2463
Size: 1545 398 274 147 411 2800
Fragments arranged by size:
2800 1545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 1178
Size: 30 165 30 648 30 4672
Fragments arranged by size:
4672 648 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2117 1186
Size: 877 54 4644
Fragments arranged by size:
4644 877 54
BseYI C'CCAG_C
Cuts at: 2112 2310 2469 2473 2606 4778 5274 2112
Size: 198 159 4 133 2172 496 2413
Fragments arranged by size:
2413 2172 496 198 159 133 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3025 3786 4814 1540
Size: 275 186 841 183 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186 183
BsiHKAI G_wGCw'C
Cuts at: 3636 3721 4792 3636
Size: 85 1071 4419
Fragments arranged by size:
4419 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2894 3636 3721 4792 1391
Size: 399 1104 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 2930 844
Size: 1185 901 3489
Fragments arranged by size:
3489 1185 901
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2837 4219 4406 1700
Size: 110 1027 1382 187 2869
Fragments arranged by size:
2869 1382 1027 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570
Size: 933 367 385 17 1452 86 12 346
Cuts at: 5570 1972
Size: 1977
Fragments arranged by size:
1977 1452 933 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2791 487
Size: 683 56 1565 3271
Fragments arranged by size:
3271 1565 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DraIII CAC_nnn'GTG
Cuts at: 2181 2181
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2839 3022
Size: 54 1425 50 275 18 405 604 183
Cuts at: 3022 3911 5540 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 604 405 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 3022 1537
Size: 275 1027 183 4090
Fragments arranged by size:
4090 1027 275 183
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2135 3505 1353
Size: 668 114 1370 3423
Fragments arranged by size:
3423 1370 668 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3638 4235 5022 5290 1239
Size: 393 2006 597 787 268 1524
Fragments arranged by size:
2006 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 2404 4722 1678
Size: 726 2318 2531
Fragments arranged by size:
2531 2318 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373
Size: 32 135 32 2644 32 42 32 2626
Fragments arranged by size:
2644 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2229 4689 4873 1305
Size: 924 2460 184 2007
Fragments arranged by size:
2460 2007 924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 10
Size: 54 1425 343 405 3348
Fragments arranged by size:
3348 1425 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2183 2546 3533 3892 4051 518
Size: 648 996 21 363 987 359 159 2042
Fragments arranged by size:
2042 996 987 648 363 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2112 2473 2991 3668 4816 5061 1606
Size: 506 361 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 518 506 361 245
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2324 2933 4478 1132
Size: 97 1095 609 1545 2229
Fragments arranged by size:
2229 1545 1095 609 97
PasI CC'CwG_GG
Cuts at: 1348 2396 1348
Size: 1048 4527
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2092 2423 1108
Size: 984 331 4260
Fragments arranged by size:
4260 984 331
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PmlI CAC'GTG
Cuts at: 2713 2713
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2196 2927 830
Size: 1366 731 3478
Fragments arranged by size:
3478 1366 731
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2112 2473 2112
Size: 361 5214
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 5575
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2192 2923 4058 4739 4930 826
Size: 219 1147 731 1135 681 191 1471
Fragments arranged by size:
1471 1147 1135 731 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2004 3014 513
Size: 552 939 1010 3074
Fragments arranged by size:
3074 1010 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2244 2519 3602 3619 3772 3957
Size: 1255 593 71 275 1083 17 153 185
Cuts at: 3957 4402 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1083 593 445 275 185 153
71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 3821 5533
Size: 274 80 33 51 1443 705 1139 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1712 1443 1139 705 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 5535 5567 2051
Size: 32 3452 32 2059
Fragments arranged by size:
3452 2059 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 2060 3704 2060
Size: 1644 3931
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI