Restriction map for
pFN21AE9275
(Circular) (Six-base) MAPSORT of: pFN21AE9275.seq Check: 3344 from: 1 to: 5509
pFN21AE9275 5509 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2405 3319 278
Size: 53 83 186 936 869 914 2468
Fragments arranged by size:
2468 936 914 869 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 2574 3572 4956 5224 1632
Size: 930 12 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 930 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AlwNI CAG_nnn'CTG
Cuts at: 2597 3252 4824 2597
Size: 655 1572 3282
Fragments arranged by size:
3282 1572 655
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2291 2818 3044 3196 5305 1493
Size: 798 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2274 4063 160
Size: 872 1242 1789 1606
Fragments arranged by size:
1789 1606 1242 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2834 1870
Size: 73 114 777 4545
Fragments arranged by size:
4545 777 114 73
BamHI G'GATC_C
Cuts at: 2204 2839 3206 2204
Size: 635 367 4507
Fragments arranged by size:
4507 635 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2830 4285 618
Size: 325 217 627 1043 1455 1842
Fragments arranged by size:
1842 1455 1043 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 2606 2828 1391
Size: 1055 160 222 4072
Fragments arranged by size:
4072 1055 222 160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4538
BbvCI CC'TCA_GC
Cuts at: 2343 2534 2343
Size: 191 5318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2369 2403 3700 3734 1903
Size: 34 432 34 1297 34 3678
Fragments arranged by size:
3678 1297 432 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2614 4169 1239
Size: 1203 172 1555 2579
Fragments arranged by size:
2579 1555 1203 172
Bpu10I CC'TnA_GC
Cuts at: 2343 2534 2343
Size: 191 5318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3634 4499 4797 3634
Size: 865 298 4346
Fragments arranged by size:
4346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2402 3316 3698 4333
Size: 53 83 186 936 869 914 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 936 914 869 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2160 2190 2377 2407 2440
Size: 30 165 30 757 30 187 30 33
Cuts at: 2440 2470 2612 2642 1178
Size: 30 142 30 4045
Fragments arranged by size:
4045 757 187 165 142 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2655 1186
Size: 1469 4040
BseYI C'CCAG_C
Cuts at: 2656 4712 5208 2656
Size: 2056 496 2957
Fragments arranged by size:
2957 2056 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4936
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 2959 3720 4748 1540
Size: 275 186 80 878 761 1028 2301
Fragments arranged by size:
2301 1028 878 761 275 186 80
BsiHKAI G_wGCw'C
Cuts at: 2446 2606 3570 3655 4726 2446
Size: 160 964 85 1071 3229
Fragments arranged by size:
3229 1071 964 160 85
BsmI GAATG_Cn'
Cuts at: 2319 3020 3113 2319
Size: 701 93 4715
Fragments arranged by size:
4715 701 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2606 2828 3570 3655 4726 1391
Size: 399 656 160 222 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 399 222 160 85
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 2278 2751 3398 5128 2278
Size: 473 647 1730 2659
Fragments arranged by size:
2659 1730 647 473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2044 4004 4178 65
Size: 1183 796 1960 174 1396
Fragments arranged by size:
1960 1396 1183 796 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2727 4153 4340 1700
Size: 110 917 1426 187 2869
Fragments arranged by size:
2869 1426 917 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2204 2839 3206 3591 3608 5060 5146 5158
Size: 232 635 367 385 17 1452 86 12
Cuts at: 5158 5504 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 635 385 367 346 232 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2051 2051
Size: 5509
BtgI C'CryG_G
Cuts at: 513 1065 2081 513
Size: 552 1016 3941
Fragments arranged by size:
3941 1016 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 2641 2800 3025 3819 3839 2581
Size: 60 159 225 794 20 4251
Fragments arranged by size:
4251 794 225 159 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2116 2813 2116
Size: 697 4812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2729 2956
Size: 54 1425 50 275 18 248 651 227
Cuts at: 2956 3845 5474 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 651 275 248 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2078 2956 1537
Size: 275 266 878 4090
Fragments arranged by size:
4090 878 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 2593 2611 3439 1353
Size: 1240 18 828 3423
Fragments arranged by size:
3423 1240 828 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2444 2604 2444
Size: 160 5349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2562 2574 2614 3572 4169 4956
Size: 393 810 120 12 40 958 597 787
Cuts at: 4956 5224 1239
Size: 268 1524
Fragments arranged by size:
1524 958 810 787 597 393 268 120
40 12
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2168 2168
Size: 5509
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 4656 1678
Size: 2978 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 2480 2512 2612
Size: 32 135 32 588 32 288 32 100
Cuts at: 2612 2644 4150 4182 4224 4256 1373
Size: 32 1506 32 42 32 2626
Fragments arranged by size:
2626 1506 588 288 135 100 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 2853 3099 669
Size: 988 281 610 305 246 3079
Fragments arranged by size:
3079 988 610 305 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 2471 2493 4623 4807 1305
Size: 1138 28 22 2130 184 2007
Fragments arranged by size:
2130 2007 1138 184 28 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2324 3467 3826 3985 518
Size: 648 1056 102 1143 359 159 2042
Fragments arranged by size:
2042 1143 1056 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2026 2083 2925 3602 4750 4995 1606
Size: 420 57 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 420 245 57
NaeI GCC'GGC
Cuts at: 2729 2729
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4957
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NgoMIV G'CCGG_C
Cuts at: 2727 2727
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2078 2956 2078
Size: 878 4631
NspI r_CATG'y
Cuts at: 1132 1229 2867 4412 1132
Size: 97 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2861 830
Size: 2031 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
SacI G_AGCT'C
Cuts at: 2446 2606 2446
Size: 160 5349
SacII CC_GC'GG
Cuts at: 2084 2084
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SapI GCTCTTCn'nnn_
Cuts at: 2593 2611 2593
Size: 18 5491
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2127 2857 3992 4673 4864 826
Size: 219 1082 730 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 730 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3613 4514 4776 820
Size: 197 926 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 2704 1517
Size: 1187 4322
StyI C'CwwG_G
Cuts at: 513 1065 2948 513
Size: 552 1883 3074
Fragments arranged by size:
3074 1883 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 3536 3553 3706 3891 4336 325
Size: 1255 650 1306 17 153 185 445 1498
Fragments arranged by size:
1498 1306 1255 650 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 5469 5501 2377
Size: 32 3060 32 2385
Fragments arranged by size:
3060 2385 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 3638 3638
Size: 5509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2403 3317 276
Size: 53 83 186 936 869 914 2468
Fragments arranged by size:
2468 936 914 869 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI