Restriction map for
pFN21AE9259
(Circular) (Six-base) MAPSORT of: pFN21AE9259.seq Check: 2651 from: 1 to: 8335
pFN21AE9259 8335 bp
With 182 enzymes: SgfI *
April 20, 2012 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4271 4923 5513 4271
Size: 652 590 7093
Fragments arranged by size:
7093 652 590
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4338 4437 4883 6145
Size: 53 83 186 936 2802 99 446 1262
Cuts at: 6145 278
Size: 2468
Fragments arranged by size:
2802 2468 1262 936 446 186 99 83
53
AccI GT'mk_AC
Cuts at: 1937 5678 1937
Size: 3741 4594
Acc65I G'GTAC_C
Cuts at: 5656 5656
Size: 8335
AclI AA'CG_TT
Cuts at: 1563 6462 6835 1563
Size: 4899 373 3063
Fragments arranged by size:
4899 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2797 2892 3648 5404 6398 7782 8050 1632
Size: 1165 95 756 1756 994 1384 268 1917
Fragments arranged by size:
1917 1756 1384 1165 994 756 268 95
AfeI AGC'GCT
Cuts at: 4685 4685
Size: 8335
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8138
AflIII A'CryG_T
Cuts at: 1203 4692 4928 7234 8211 1203
Size: 3489 236 2306 977 1327
Fragments arranged by size:
3489 2306 1327 977 236
AgeI A'CCGG_T
Cuts at: 7166 7166
Size: 8335
AhdI GACnn_n'nnGTC
Cuts at: 2773 2866 7064 2773
Size: 93 4198 4044
Fragments arranged by size:
4198 4044 93
AleI CACnn'nnGTG
Cuts at: 1166 4931 5346 1166
Size: 3765 415 4155
Fragments arranged by size:
4155 3765 415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4085 5132 1184
Size: 2901 1047 4387
Fragments arranged by size:
4387 2901 1047
AlwNI CAG_nnn'CTG
Cuts at: 2210 2498 3692 6078 7650 2210
Size: 288 1194 2386 1572 2895
Fragments arranged by size:
2895 2386 1572 1194 288
ApaI G_GGCC'C
Cuts at: 3828 3828
Size: 8335
ApaLI G'TGCA_C
Cuts at: 6392 7548 6392
Size: 1156 7179
ApoI r'AATT_y
Cuts at: 1493 5018 5644 5870 6022 8131 1493
Size: 3525 626 226 152 2109 1697
Fragments arranged by size:
3525 2109 1697 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6889 160
Size: 872 5857 1606
Fragments arranged by size:
5857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 3487 3651 3722 4581 4679 4785
Size: 73 828 716 164 71 859 98 106
Cuts at: 4785 5427 5603 5660 1870
Size: 642 176 57 4545
Fragments arranged by size:
4545 859 828 716 642 176 164 106
98 73 71 57
BamHI G'GATC_C
Cuts at: 2322 5665 6032 2322
Size: 3343 367 4625
Fragments arranged by size:
4625 3343 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 3515 3780 4427 5656
Size: 325 217 627 1511 217 265 647 1229
Cuts at: 5656 7111 618
Size: 1455 1842
Fragments arranged by size:
1842 1511 1455 1229 647 627 325 265
217 217
BanII G_rGCy'C
Cuts at: 1391 2476 2525 2554 3137 3828 4094 4839 5610
Size: 1085 49 29 583 691 266 745 771
Cuts at: 5610 5654 1391
Size: 44 4072
Fragments arranged by size:
4072 1085 771 745 691 583 266 49
44 29
BbeI G_GCGC'C
Cuts at: 3784 4431 3784
Size: 647 7688
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3589 5368 928
Size: 971 1690 1779 3895
Fragments arranged by size:
3895 1779 1690 971
BbvCI CC'TCA_GC
Cuts at: 5229 5229
Size: 8335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5531 5565 6526 6560 1903
Size: 34 3594 34 961 34 3678
Fragments arranged by size:
3678 3594 961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6227 7437 6227
Size: 1210 7125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3880 4965
Size: 107 122 71 631 605 337 1871 1085
Cuts at: 4965 5514 6946 136
Size: 549 1432 1525
Fragments arranged by size:
1871 1525 1432 1085 631 605 549 337
122 107 71
BglII A'GATC_T
Cuts at: 2671 4101 8330 2671
Size: 1430 4229 2676
Fragments arranged by size:
4229 2676 1430
BlpI GC'TnA_GC
Cuts at: 4316 4973 5752 4316
Size: 657 779 6899
Fragments arranged by size:
6899 779 657
Bme1580I G_kGCm'C
Cuts at: 1790 3336 3518 3754 3828 4806 6396 7552 1790
Size: 1546 182 236 74 978 1590 1156 2573
Fragments arranged by size:
2573 1590 1546 1156 978 236 182 74
BmgBI CAC'GTC
Cuts at: 1318 4693 4780 1318
Size: 3375 87 4873
Fragments arranged by size:
4873 3375 87
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2663 3141 3741
Size: 416 53 83 399 24 1246 478 600
Cuts at: 3741 3887 4483 5451 6066 7024 442
Size: 146 596 968 615 958 1753
Fragments arranged by size:
1753 1246 968 958 615 600 596 478
416 399 146 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5236 5629 5236
Size: 393 7942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2187 2201 2493 2801 3200 3266 3545
Size: 863 85 14 292 308 399 66 279
Cuts at: 3545 4146 4217 4997 5095 5576 6995 1239
Size: 601 71 780 98 481 1419 2579
Fragments arranged by size:
2579 1419 863 780 601 481 399 308
292 279 98 85 71 66 14
Bpu10I CC'TnA_GC
Cuts at: 3820 4635 5229 5592 3820
Size: 815 594 363 6563
Fragments arranged by size:
6563 815 594 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2312 3380 3618 6460 7325 7623 2312
Size: 1068 238 2842 865 298 3024
Fragments arranged by size:
3024 2842 1068 865 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3398 3443 6998 882
Size: 617 1899 45 3555 2219
Fragments arranged by size:
3555 2219 1899 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 4622 4929 6126 493
Size: 711 3418 307 1197 2702
Fragments arranged by size:
3418 2702 1197 711 307
BsaBI GATnn'nnATC
Cuts at: 8329 8329
Size: 8335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4335 4364
Size: 53 83 186 936 2248 382 172 29
Cuts at: 4364 4428 4434 4880 6142 6524 7159 275
Size: 64 6 446 1262 382 635 1451
Fragments arranged by size:
2248 1451 1262 936 635 446 382 382
186 172 83 64 53 29 6
BsaWI w'CCGG_w
Cuts at: 4980 5436 6768 7166 7440 7587 7998 4980
Size: 456 1332 398 274 147 411 5317
Fragments arranged by size:
5317 1332 456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3673 3703 4463 4493 5441
Size: 30 165 30 2270 30 760 30 948
Cuts at: 5441 5471 1178
Size: 30 4042
Fragments arranged by size:
4042 2270 948 760 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 2732 2798 2921 2924 2927 2936 2960
Size: 926 620 66 123 3 3 9 24
Cuts at: 2960 2963 2993 3127 3132 3281 4262 4469 5462
Size: 3 30 134 5 149 981 207 993
Cuts at: 5462 1186
Size: 4059
Fragments arranged by size:
4059 993 981 926 620 207 149 134
123 66 30 24 9 5 3 3
3
BseYI C'CCAG_C
Cuts at: 2329 2363 3188 3218 5151 7538 8034 2329
Size: 34 825 30 1933 2387 496 2630
Fragments arranged by size:
2630 2387 1933 825 496 34 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3301 3490 3678 4238 4454 4553 4909
Size: 573 1565 189 188 560 216 99 356
Cuts at: 4909 1163
Size: 4589
Fragments arranged by size:
4589 1565 573 560 356 216 189 188
99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 3678 4900 5473 5785 6546
Size: 275 186 39 1638 1222 573 312 761
Cuts at: 6546 7574 1540
Size: 1028 2301
Fragments arranged by size:
2301 1638 1222 1028 761 573 312 275
186 39
BsiHKAI G_wGCw'C
Cuts at: 3444 4322 4389 5069 5610 6396 6481 7552 3444
Size: 878 67 680 541 786 85 1071 4227
Fragments arranged by size:
4227 1071 878 786 680 541 85 67
BsmI GAATG_Cn'
Cuts at: 4525 5846 5939 4525
Size: 1321 93 6921
Fragments arranged by size:
6921 1321 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2786 3075 4871 2786
Size: 289 1796 6250
Fragments arranged by size:
6250 1796 289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2476 2525 2554 3137 3336 3444 3518
Size: 399 686 49 29 583 199 108 74
Cuts at: 3518 3754 3828 4094 4322 4389 4806 4839 5069
Size: 236 74 266 228 67 417 33 230
Cuts at: 5069 5610 5654 6396 6481 7552 1391
Size: 541 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 686 583 541 417 399
266 236 230 228 199 108 85 74
74 67 49 44 33 29
BspEI T'CCGG_A
Cuts at: 5436 7998 5436
Size: 2562 5773
BspHI T'CATG_A
Cuts at: 6224 7954 6224
Size: 1730 6605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4271 4923 5403 5513 5690 844
Size: 3427 652 480 110 177 3489
Fragments arranged by size:
3489 3427 652 480 177 110
BsrBI CCG'CTC
Cuts at: 1394 1823 6222 1394
Size: 429 4399 3507
Fragments arranged by size:
4399 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3388 5547 6830 7004 65
Size: 1183 2140 2159 1283 174 1396
Fragments arranged by size:
2159 2140 1396 1283 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3018 3673 3677 6979 7166 1700
Size: 110 1208 655 4 3302 187 2869
Fragments arranged by size:
3302 2869 1208 655 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8335
BssHII G'CGCG_C
Cuts at: 1925 4523 1925
Size: 2598 5737
BssSI C'ACGA_G
Cuts at: 2518 3114 6395 7407 2518
Size: 596 3281 1012 3446
Fragments arranged by size:
3446 3281 1012 596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3692 4983 794
Size: 2898 1291 4146
Fragments arranged by size:
4146 2898 1291
BstBI TT'CG_AA
Cuts at: 7139 7139
Size: 8335
BstEII G'GTnAC_C
Cuts at: 1184 2662 3640 3664 1184
Size: 1478 978 24 5855
Fragments arranged by size:
5855 1478 978 24
BstXI CCAn_nnnn'nTGG
Cuts at: 3427 4352 5554 3427
Size: 925 1202 6208
Fragments arranged by size:
6208 1202 925
BstYI r'GATC_y
Cuts at: 1972 2322 2671 4101 5665 6032 6417 6434 7886
Size: 350 349 1430 1564 367 385 17 1452
Cuts at: 7886 7972 7984 8330 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1564 1452 1430 385 367 350 349
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3139 4845 3139
Size: 1706 6629
BtgI C'CryG_G
Cuts at: 513 1065 2004 2311 3056 3098 3864 4095 4179
Size: 552 939 307 745 42 766 231 84
Cuts at: 4179 4552 513
Size: 373 4296
Fragments arranged by size:
4296 939 766 745 552 373 307 231
84 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4486 4558 5395 487
Size: 683 56 3260 72 837 3427
Fragments arranged by size:
3427 3260 837 683 72 56
BtsI GCAGTG_nn'
Cuts at: 3478 3681 4149 4921 5851 6645 6665 3478
Size: 203 468 772 930 794 20 5148
Fragments arranged by size:
5148 930 794 772 468 203 20
ClaI AT'CG_AT
Cuts at: 1554 4896 1554
Size: 3342 4993
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8300
DraI TTT'AAA
Cuts at: 5639 5639
Size: 8335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7342 809
Size: 507 6026 1802
Fragments arranged by size:
6026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2676 3016
Size: 54 1425 50 275 18 207 639 340
Cuts at: 3016 3345 3675 4966 5782 6671 8300 8
Size: 329 330 1291 816 889 1629 43
Fragments arranged by size:
1629 1425 1291 889 816 639 340 330
329 275 207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 3675 5782 1537
Size: 275 225 1638 2107 4090
Fragments arranged by size:
4090 2107 1638 275 225
EarI CTCTTCn'nnn_
Cuts at: 1353 2362 2898 2913 2931 2937 2949 2961 5267
Size: 1009 536 15 18 6 12 12 2306
Cuts at: 5267 6265 1353
Size: 998 3423
Fragments arranged by size:
3423 2306 1009 998 536 18 15 12
12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 6922 7296 7442 387
Size: 1064 138 2360 2973 374 146 1280
Fragments arranged by size:
2973 2360 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5608 5608
Size: 8335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2187 2201 2493 2797 2801 2892
Size: 393 470 85 14 292 304 4 91
Cuts at: 2892 3200 3266 3545 3648 4146 4217 4997 5095
Size: 308 66 279 103 498 71 780 98
Cuts at: 5095 5404 5576 6398 6995 7782 8050 1239
Size: 309 172 822 597 787 268 1524
Fragments arranged by size:
1524 822 787 780 597 498 470 393
309 308 304 292 279 268 172 103
98 91 85 71 66 14 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2714 3710 3902 2159
Size: 555 996 192 6592
Fragments arranged by size:
6592 996 555 192
EcoO109I rG'GnC_Cy
Cuts at: 2053 2305 2767 2775 3143 3163 3174 3198 3824
Size: 252 462 8 368 20 11 24 626
Cuts at: 3824 3825 3895 3907 4669 5187 2053
Size: 1 70 12 762 518 5201
Fragments arranged by size:
5201 762 626 518 462 368 252 70
24 20 12 11 8 1
EcoRI G'AATT_C
Cuts at: 5644 6022 5644
Size: 378 7957
FspI TGC'GCA
Cuts at: 6841 6841
Size: 8335
HaeII r_GCGC'y
Cuts at: 1678 2075 3784 4431 4497 4687 7482 1678
Size: 397 1709 647 66 190 2795 2531
Fragments arranged by size:
2795 2531 1709 647 397 190 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2656 2665 2688 2759
Size: 32 135 32 1061 23 9 23 71
Cuts at: 2759 2791 2819 2851 2852 2884 2966 2998 4928
Size: 32 28 32 1 32 82 32 1930
Cuts at: 4928 4960 5020 5052 6976 7008 7050 7082 1373
Size: 32 60 32 1924 32 42 32 2626
Fragments arranged by size:
2626 1930 1924 1061 135 82 71 60
42 32 32 32 32 32 32 32
32 32 32 28 23 23 9 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4339 4546 4819 5629 5679 5925
Size: 988 281 2401 207 273 810 50 246
Cuts at: 5925 669
Size: 3079
Fragments arranged by size:
3079 2401 988 810 281 273 246 207
50
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8335
HpaI GTT'AAC
Cuts at: 5925 5925
Size: 8335
KasI G'GCGC_C
Cuts at: 3780 4427 3780
Size: 647 7688
KpnI G_GTAC'C
Cuts at: 5660 5660
Size: 8335
MfeI C'AATT_G
Cuts at: 5934 5934
Size: 8335
MluI A'CGCG_T
Cuts at: 8211 8211
Size: 8335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 2883 3996 4713 4750 4977 7449 7633
Size: 1389 189 1113 717 37 227 2472 184
Cuts at: 7633 1305
Size: 2007
Fragments arranged by size:
2472 2007 1389 1113 717 227 189 184
37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2678 3347 10
Size: 54 1425 343 846 669 4998
Fragments arranged by size:
4998 1425 846 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4400 4931 5114 5346 6293 6652 6811
Size: 648 3234 531 183 232 947 359 159
Cuts at: 6811 518
Size: 2042
Fragments arranged by size:
3234 2042 947 648 531 359 232 183
159
MspA1I CmG'CkG
Cuts at: 1606 2030 2060 3100 3285 3583 4159 5041 5751
Size: 424 30 1040 185 298 576 882 710
Cuts at: 5751 6428 7576 7821 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 882 710 677 576 424
298 245 185 30
NaeI GCC'GGC
Cuts at: 3020 3679 3020
Size: 659 7676
NarI GG'CG_CC
Cuts at: 3781 4428 3781
Size: 647 7688
NcoI C'CATG_G
Cuts at: 513 1065 2004 3056 4552 513
Size: 552 939 1052 1496 4296
Fragments arranged by size:
4296 1496 1052 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8335
NgoMIV G'CCGG_C
Cuts at: 3018 3677 3018
Size: 659 7676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8335
NotI GC'GGCC_GC
Cuts at: 2037 5782 2037
Size: 3745 4590
NspI r_CATG'y
Cuts at: 1132 1229 4076 4414 5693 7238 1132
Size: 97 2847 338 1279 1545 2229
Fragments arranged by size:
2847 2229 1545 1279 338 97
PasI CC'CwG_GG
Cuts at: 1348 2476 1348
Size: 1128 7207
PciI A'CATG_T
Cuts at: 7234 7234
Size: 8335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2270 2443 2579 2653 4374 1108
Size: 1162 173 136 74 1721 5069
Fragments arranged by size:
5069 1721 1162 173 136 74
PfoI T'CCnGG_A
Cuts at: 3522 3918 5013 3522
Size: 396 1095 6844
Fragments arranged by size:
6844 1095 396
PmeI GTTT'AAAC
Cuts at: 5639 5639
Size: 8335
PmlI CAC'GTG
Cuts at: 4622 4929 4622
Size: 307 8028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6404 6436 7917 7949 6404
Size: 32 1481 32 6790
Fragments arranged by size:
6790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2767 2775 3163 3174 3198 4669 5187 2305
Size: 462 8 388 11 24 1471 518 5453
Fragments arranged by size:
5453 1471 518 462 388 24 11 8
PshAI GACnn'nnGTC
Cuts at: 1613 2814 1613
Size: 1201 7134
PsiI TTA'TAA
Cuts at: 5905 5905
Size: 8335
PspOMI G'GGCC_C
Cuts at: 3824 3824
Size: 8335
PstI C_TGCA'G
Cuts at: 830 2029 2488 3688 3996 4122 4384 4920 5172
Size: 1199 459 1200 308 126 262 536 252
Cuts at: 5172 5687 830
Size: 515 3478
Fragments arranged by size:
3478 1200 1199 536 515 459 308 262
252 126
PvuI CG_AT'CG
Cuts at: 2001 4900 2001
Size: 2899 5436
PvuII CAG'CTG
Cuts at: 3285 3583 3285
Size: 298 8037
SacI G_AGCT'C
Cuts at: 5610 5610
Size: 8335
SacII CC_GC'GG
Cuts at: 3101 3101
Size: 8335
SalI G'TCGA_C
Cuts at: 1936 5677 1936
Size: 3741 4594
SanDI GG'GwC_CC
Cuts at: 2767 2775 3174 3198 2767
Size: 8 399 24 7904
Fragments arranged by size:
7904 399 24 8
SbfI CC_TGCA'GG
Cuts at: 5687 5687
Size: 8335
ScaI AGT'ACT
Cuts at: 6583 8295 6583
Size: 1712 6623
SfcI C'TryA_G
Cuts at: 826 1045 2025 2250 2484 3684 3992 4118 4237
Size: 219 980 225 234 1200 308 126 119
Cuts at: 4237 4380 4916 5123 5168 5683 6818 7499 7690
Size: 143 536 207 45 515 1135 681 191
Cuts at: 7690 826
Size: 1471
Fragments arranged by size:
1471 1200 1135 980 681 536 515 308
234 225 219 207 191 143 126 119
45
SfoI GGC'GCC
Cuts at: 3782 4429 3782
Size: 647 7688
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8335
SmaI CCC'GGG
Cuts at: 1872 2773 3489 3653 3724 5662 1872
Size: 901 716 164 71 1938 4545
Fragments arranged by size:
4545 1938 901 716 164 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2291 3395 3633 6439 7340 7602
Size: 197 926 348 1104 238 2806 901 262
Cuts at: 7602 820
Size: 1553
Fragments arranged by size:
2806 1553 1104 926 901 348 262 238
197
SnaBI TAC'GTA
Cuts at: 493 6126 493
Size: 5633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8335
SphI G_CATG'C
Cuts at: 1132 4076 4414 5693 1132
Size: 2944 338 1279 3774
Fragments arranged by size:
3774 2944 1279 338
SrfI GCCC'GGGC
Cuts at: 3489 3489
Size: 8335
SspI AAT'ATT
Cuts at: 5 52 5498 6259 5
Size: 47 5446 761 2081
Fragments arranged by size:
5446 2081 761 47
StuI AGG'CCT
Cuts at: 1517 3569 1517
Size: 2052 6283
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2538 3056 3105 4552 5774
Size: 552 939 156 378 518 49 1447 1222
Cuts at: 5774 513
Size: 3074
Fragments arranged by size:
3074 1447 1222 939 552 518 378 156
49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2792 3049 3316 4925 6362 6379 6532
Size: 1255 1212 257 267 1609 1437 17 153
Cuts at: 6532 6717 7162 325
Size: 185 445 1498
Fragments arranged by size:
1609 1498 1437 1255 1212 445 267 257
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3153 4857 5292
Size: 274 80 33 51 1443 1176 1704 435
Cuts at: 5292 6581 8293 96
Size: 1289 1712 138
Fragments arranged by size:
1712 1704 1443 1289 1176 435 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3673 3705 4463 4495 8295 8327 3673
Size: 32 758 32 3800 32 3681
Fragments arranged by size:
3800 3681 758 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8335
XbaI T'CTAG_A
Cuts at: 5671 5671
Size: 8335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3154 3355 3154
Size: 201 8134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8335
XmaI C'CCGG_G
Cuts at: 1870 2771 3487 3651 3722 5660 1870
Size: 901 716 164 71 1938 4545
Fragments arranged by size:
4545 1938 901 716 164 71
XmnI GAAnn'nnTTC
Cuts at: 4512 6464 4512
Size: 1952 6383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4336 4435 4881 6143
Size: 53 83 186 936 2802 99 446 1262
Cuts at: 6143 276
Size: 2468
Fragments arranged by size:
2802 2468 1262 936 446 186 99 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SapI SexAI SfiI SwaI