Restriction map for
pFN21AE9258
(Circular) (Six-base) MAPSORT of: pFN21AE9258.seq Check: 4139 from: 1 to: 5563
pFN21AE9258 5563 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 2627 2906 1937
Size: 493 197 279 4594
Fragments arranged by size:
4594 493 279 197
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 2397 4462 5439 1203
Size: 1194 2065 977 1327
Fragments arranged by size:
2065 1327 1194 977
AgeI A'CCGG_T
Cuts at: 2157 4394 2157
Size: 2237 3326
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 3306 4878 3306
Size: 1572 3991
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2562 2872 3098 3250 5359 1493
Size: 1069 310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1069 310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2888 1870
Size: 73 945 4545
Fragments arranged by size:
4545 945 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 2884 4339 618
Size: 325 217 627 590 507 1455 1842
Fragments arranged by size:
1842 1455 627 590 507 325 217
BanII G_rGCy'C
Cuts at: 1391 2882 1391
Size: 1491 4072
BbeI G_GCGC'C
Cuts at: 2381 2381
Size: 5563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2702 2865 928
Size: 971 803 163 3626
Fragments arranged by size:
3626 971 803 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 2718 1548
Size: 1170 4393
BfrBI ATG'CAT
Cuts at: 2782 2782
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2064 4174 136
Size: 107 122 71 631 605 392 2110 1525
Fragments arranged by size:
2110 1525 631 605 392 122 107 71
BglII A'GATC_T
Cuts at: 5558 5558
Size: 5563
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 3624 4780 1790
Size: 1834 1156 2573
Fragments arranged by size:
2573 1834 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2503 2674 3294
Size: 416 53 83 399 24 1086 171 620
Cuts at: 3294 4252 442
Size: 958 1753
Fragments arranged by size:
1753 1086 958 620 416 399 171 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2434 2765 4223 1239
Size: 1128 67 331 1458 2579
Fragments arranged by size:
2579 1458 1128 331 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2378 3370 3752 4387
Size: 53 83 186 936 845 992 382 635
Cuts at: 4387 275
Size: 1451
Fragments arranged by size:
1451 992 936 845 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2157 3996 4394 4668 4815 5226 2157
Size: 1839 398 274 147 411 2494
Fragments arranged by size:
2494 1839 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 1178
Size: 30 165 30 1003 30 4305
Fragments arranged by size:
4305 1003 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 1186
Size: 927 4636
BseYI C'CCAG_C
Cuts at: 4766 5262 4766
Size: 496 5067
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2161 2724 1163
Size: 573 425 563 4002
Fragments arranged by size:
4002 573 563 425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 3624 3709 4780 2149
Size: 1475 85 1071 2932
Fragments arranged by size:
2932 1475 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2882 3624 3709 4780 1391
Size: 399 359 733 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 399 359 85
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 844
Size: 2074 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2558 3450 1394
Size: 429 735 892 3507
Fragments arranged by size:
3507 892 735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2818 4058 4232 65
Size: 1183 1570 1240 174 1396
Fragments arranged by size:
1570 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2157 2374 4207 4394 1700
Size: 110 347 217 1833 187 2869
Fragments arranged by size:
2869 1833 347 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 3623 4635 3623
Size: 1012 4551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5563
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558
Size: 921 367 385 17 1452 86 12 346
Cuts at: 5558 1972
Size: 1977
Fragments arranged by size:
1977 1452 921 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2628 2628
Size: 5563
BtgI C'CryG_G
Cuts at: 513 1065 2062 513
Size: 552 997 4014
Fragments arranged by size:
4014 997 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4824
Fragments arranged by size:
4824 683 56
BtsI GCAGTG_nn'
Cuts at: 3079 3873 3893 3079
Size: 794 20 4749
Fragments arranged by size:
4749 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2514 2867 2514
Size: 353 5210
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2423 4570 809
Size: 507 1107 2147 1802
Fragments arranged by size:
2147 1802 1107 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 3010 3899
Size: 54 1425 50 275 18 213 967 889
Cuts at: 3899 5528 8
Size: 1629 43
Fragments arranged by size:
1629 1425 967 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3493 1353
Size: 2140 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4150 4524 4670 387
Size: 1064 138 2561 374 146 1280
Fragments arranged by size:
2561 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2434 2765 3626 4223 5010 5278
Size: 393 735 67 331 861 597 787 268
Cuts at: 5278 1239
Size: 1524
Fragments arranged by size:
1524 861 787 735 597 393 331 268
67
EcoNI CCTnn'n_nnAGG
Cuts at: 2215 2215
Size: 5563
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 2381 4710 1678
Size: 703 2329 2531
Fragments arranged by size:
2531 2329 703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2406 2438 2466 2498 4204
Size: 32 135 32 834 32 28 32 1706
Cuts at: 4204 4236 4278 4310 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1706 834 135 42 32 32 32
32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KasI G'GCGC_C
Cuts at: 2377 2377
Size: 5563
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2657 4677 4861 1305
Size: 1352 2020 184 2007
Fragments arranged by size:
2020 2007 1352 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3741
Fragments arranged by size:
3741 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3521 3880 4039 518
Size: 648 2355 359 159 2042
Fragments arranged by size:
2355 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2979 3656 4804 5049 1606
Size: 458 915 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 458 245
NaeI GCC'GGC
Cuts at: 2376 2376
Size: 5563
NarI GG'CG_CC
Cuts at: 2378 2378
Size: 5563
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5011
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NgoMIV G'CCGG_C
Cuts at: 2374 2374
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NsiI A_TGCA'T
Cuts at: 2784 2784
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2786 2921 4466 1132
Size: 97 1557 135 1545 2229
Fragments arranged by size:
2229 1557 1545 135 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4153
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2315 2764 1108
Size: 902 305 449 3907
Fragments arranged by size:
3907 902 449 305
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2117 1613
Size: 504 5059
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2915 830
Size: 2085 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
SacII CC_GC'GG
Cuts at: 2065 2065
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2911 4046 4727 4918 826
Size: 219 1866 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2379 2379
Size: 5563
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 2204 1517
Size: 687 4876
StyI C'CwwG_G
Cuts at: 513 1065 2040 3002 513
Size: 552 975 962 3074
Fragments arranged by size:
3074 975 962 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2679 3590 3607 3760 3945 4390 325
Size: 1255 1099 911 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1099 911 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3809 5521 96
Size: 274 80 33 51 1443 1832 1712 138
Fragments arranged by size:
1832 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 2759 2791 5523 5555 2686
Size: 32 41 32 2732 32 2694
Fragments arranged by size:
2732 2694 41 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2420 1316
Size: 1104 4459
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 3692 3692
Size: 5563
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI