Restriction map for
pFN21AE9255
(Circular) (Six-base) MAPSORT of: pFN21AE9255.seq Check: 9626 from: 1 to: 8296
pFN21AE9255 8296 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 8296
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6106 278
Size: 53 83 186 936 4570 2468
Fragments arranged by size:
4570 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2887 3666 5639 1937
Size: 950 779 1973 4594
Fragments arranged by size:
4594 1973 950 779
Acc65I G'GTAC_C
Cuts at: 5617 5617
Size: 8296
AclI AA'CG_TT
Cuts at: 1563 3810 5466 6423 6796 1563
Size: 2247 1656 957 373 3063
Fragments arranged by size:
3063 2247 1656 957 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 2777 3135 3330 3836 4194 4797 4895
Size: 1119 26 358 195 506 358 603 98
Cuts at: 4895 5007 6359 7743 8011 1632
Size: 112 1352 1384 268 1917
Fragments arranged by size:
1917 1384 1352 1119 603 506 358 358
268 195 112 98 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8099
AflIII A'CryG_T
Cuts at: 1203 5584 7195 8172 1203
Size: 4381 1611 977 1327
Fragments arranged by size:
4381 1611 1327 977
AgeI A'CCGG_T
Cuts at: 7127 7127
Size: 8296
AhdI GACnn_n'nnGTC
Cuts at: 5128 7025 5128
Size: 1897 6399
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3162 1184
Size: 1978 6318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2277 2309 2277
Size: 32 8264
AlwNI CAG_nnn'CTG
Cuts at: 4358 4985 5395 6039 7611 4358
Size: 627 410 644 1572 5043
Fragments arranged by size:
5043 1572 644 627 410
ApaLI G'TGCA_C
Cuts at: 6353 7509 6353
Size: 1156 7140
ApoI r'AATT_y
Cuts at: 1493 3744 5046 5605 5831 5983 8092 1493
Size: 2251 1302 559 226 152 2109 1697
Fragments arranged by size:
2251 2109 1697 1302 559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4827 5294 6850 160
Size: 872 3795 467 1556 1606
Fragments arranged by size:
3795 1606 1556 872 467
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8296
AvaI C'yCGr_G
Cuts at: 1870 1943 5621 1870
Size: 73 3678 4545
Fragments arranged by size:
4545 3678 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2727 2760 2727
Size: 33 8263
BamHI G'GATC_C
Cuts at: 3538 5626 5993 3538
Size: 2088 367 5841
Fragments arranged by size:
5841 2088 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5617 7072 618
Size: 325 217 627 3830 1455 1842
Fragments arranged by size:
3830 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4096 4491 5615 1391
Size: 2705 395 1124 4072
Fragments arranged by size:
4072 2705 1124 395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5070 928
Size: 971 3171 4154
Fragments arranged by size:
4154 3171 971
BbvCI CC'TCA_GC
Cuts at: 2754 4581 2754
Size: 1827 6469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3443 3477 6487 6521 1903
Size: 34 50 34 1422 34 3010 34 3678
Fragments arranged by size:
3678 3010 1422 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4807 6188 7398 4807
Size: 1381 1210 5705
Fragments arranged by size:
5705 1381 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8296
BfrBI ATG'CAT
Cuts at: 2008 4424 2008
Size: 2416 5880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3257 6907 136
Size: 107 122 71 631 605 1585 3650 1525
Fragments arranged by size:
3650 1585 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8291 8291
Size: 8296
BlpI GC'TnA_GC
Cuts at: 2574 2749 2922 5713 2574
Size: 175 173 2791 5157
Fragments arranged by size:
5157 2791 175 173
Bme1580I G_kGCm'C
Cuts at: 1790 6357 7513 1790
Size: 4567 1156 2573
Fragments arranged by size:
4567 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6027 6985 442
Size: 416 53 83 399 24 4610 958 1753
Fragments arranged by size:
4610 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3801 3665
Size: 136 8160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2901 3660 4187 5228 6956 1239
Size: 1224 438 759 527 1041 1728 2579
Fragments arranged by size:
2579 1728 1224 1041 759 527 438
Bpu10I CC'TnA_GC
Cuts at: 2754 4581 2754
Size: 1827 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2945 4475 4602 6421 7286 7584 2945
Size: 1530 127 1819 865 298 3657
Fragments arranged by size:
3657 1819 1530 865 298 127
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6959 882
Size: 617 5460 2219
Fragments arranged by size:
5460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6087 493
Size: 711 4883 2702
Fragments arranged by size:
4883 2702 711
BsaBI GATnn'nnATC
Cuts at: 8290 8290
Size: 8296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6103 6485 7120 275
Size: 53 83 186 936 4570 382 635 1451
Fragments arranged by size:
4570 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6729 7127 7401 7548 7959 6729
Size: 398 274 147 411 7066
Fragments arranged by size:
7066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3954 3984 5571 5601 1178
Size: 30 165 30 2551 30 1587 30 3873
Fragments arranged by size:
3873 2551 1587 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4286 1186
Size: 3100 5196
BseYI C'CCAG_C
Cuts at: 2149 7499 7995 2149
Size: 5350 496 2450
Fragments arranged by size:
5350 2450 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2529 3114 1163
Size: 573 793 585 6345
Fragments arranged by size:
6345 793 585 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5746 6507 7535 1540
Size: 275 186 3745 761 1028 2301
Fragments arranged by size:
3745 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4006 4491 5584 6357 6442 7513 4006
Size: 485 1093 773 85 1071 4789
Fragments arranged by size:
4789 1093 1071 773 485 85
BsmI GAATG_Cn'
Cuts at: 5807 5900 5807
Size: 93 8203
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4006 4096 4491 5584 5615 6357 6442
Size: 399 2216 90 395 1093 31 742 85
Cuts at: 6442 7513 1391
Size: 1071 2174
Fragments arranged by size:
2216 2174 1093 1071 742 399 395 90
85 31
BspEI T'CCGG_A
Cuts at: 7959 7959
Size: 8296
BspHI T'CATG_A
Cuts at: 2296 2839 6185 7915 2296
Size: 543 3346 1730 2677
Fragments arranged by size:
3346 2677 1730 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 4103 5329 5651 844
Size: 1467 1792 1226 322 3489
Fragments arranged by size:
3489 1792 1467 1226 322
BsrBI CCG'CTC
Cuts at: 1394 1823 6183 1394
Size: 429 4360 3507
Fragments arranged by size:
4360 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3867 6791 6965 65
Size: 1183 2619 2924 174 1396
Fragments arranged by size:
2924 2619 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6940 7127 1700
Size: 110 5130 187 2869
Fragments arranged by size:
5130 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8296
BssSI C'ACGA_G
Cuts at: 3462 6356 7368 3462
Size: 2894 1012 4390
Fragments arranged by size:
4390 2894 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3197 794
Size: 2403 5893
BstBI TT'CG_AA
Cuts at: 7100 7100
Size: 8296
BstEII G'GTnAC_C
Cuts at: 1184 4627 1184
Size: 3443 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 3919 5384 5488 2128
Size: 1791 1465 104 4936
Fragments arranged by size:
4936 1791 1465 104
BstYI r'GATC_y
Cuts at: 1972 2389 3538 5362 5626 5993 6378 6395 7847
Size: 417 1149 1824 264 367 385 17 1452
Cuts at: 7847 7933 7945 8291 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1824 1452 1149 417 385 367 346
264 86 17 12
BstZ17I GTA'TAC
Cuts at: 2888 2888
Size: 8296
Bsu36I CC'TnA_GG
Cuts at: 5224 5224
Size: 8296
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7744
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7557
Fragments arranged by size:
7557 683 56
BtsI GCAGTG_nn'
Cuts at: 4125 4163 5812 6606 6626 4125
Size: 38 1649 794 20 5795
Fragments arranged by size:
5795 1649 794 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2304 2339 529
Size: 35 1740 35 6486
Fragments arranged by size:
6486 1740 35 35
DraI TTT'AAA
Cuts at: 5600 5600
Size: 8296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7303 809
Size: 507 5987 1802
Fragments arranged by size:
5987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4849 5743 6632
Size: 54 1425 50 275 18 3019 894 889
Cuts at: 6632 8261 8
Size: 1629 43
Fragments arranged by size:
3019 1629 1425 894 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5743 1537
Size: 275 3931 4090
Fragments arranged by size:
4090 3931 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2399 2601 4095 4170 4479 4617 4983
Size: 734 312 202 1494 75 309 138 366
Cuts at: 4983 5012 5318 6226 1353
Size: 29 306 908 3423
Fragments arranged by size:
3423 1494 908 734 366 312 309 306
202 138 75 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6883 7257 7403 387
Size: 1064 138 5294 374 146 1280
Fragments arranged by size:
5294 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4489 4489
Size: 8296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2751 2777 2901 3135 3330 3660
Size: 393 831 288 26 124 234 195 330
Cuts at: 3660 3836 4187 4194 4797 4895 5007 5228 6359
Size: 176 351 7 603 98 112 221 1131
Cuts at: 6359 6956 7743 8011 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1131 831 787 603 597 393 351
330 288 268 234 221 195 176 124
112 98 26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 5342 2168
Size: 3174 5122
EcoRI G'AATT_C
Cuts at: 5605 5983 5605
Size: 378 7918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2927 3212 3401 5239 2927
Size: 285 189 1838 5984
Fragments arranged by size:
5984 1838 285 189
FspI TGC'GCA
Cuts at: 6802 6802
Size: 8296
HaeII r_GCGC'y
Cuts at: 1678 7443 1678
Size: 5765 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 2630 2662 4490
Size: 32 135 32 902 32 124 32 1828
Cuts at: 4490 4522 5114 5146 6937 6969 7011 7043 1373
Size: 32 592 32 1791 32 42 32 2626
Fragments arranged by size:
2626 1828 1791 902 592 135 124 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 5640 5886 669
Size: 988 281 481 3221 246 3079
Fragments arranged by size:
3221 3079 988 481 281 246
HindIII A'AGCT_T
Cuts at: 748 2789 748
Size: 2041 6255
HpaI GTT'AAC
Cuts at: 2419 5886 2419
Size: 3467 4829
KpnI G_GTAC'C
Cuts at: 5621 5621
Size: 8296
MfeI C'AATT_G
Cuts at: 5895 5895
Size: 8296
MluI A'CGCG_T
Cuts at: 8172 8172
Size: 8296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2184 2642 7410 7594 1305
Size: 840 39 458 4768 184 2007
Fragments arranged by size:
4768 2007 840 458 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4851 10
Size: 54 1425 343 3019 3455
Fragments arranged by size:
3455 3019 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3144 5429 6254 6613 6772 518
Size: 648 1978 2285 825 359 159 2042
Fragments arranged by size:
2285 2042 1978 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2510 2753 3128 3158 3457 4580 5712 6389
Size: 904 243 375 30 299 1123 1132 677
Cuts at: 6389 7537 7782 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1132 1123 904 677 375 299
245 243 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7744
NdeI CA'TA_TG
Cuts at: 387 3490 387
Size: 3103 5193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8296
NotI GC'GGCC_GC
Cuts at: 5743 5743
Size: 8296
NsiI A_TGCA'T
Cuts at: 2010 4426 2010
Size: 2416 5880
NspI r_CATG'y
Cuts at: 1132 1229 2982 3086 3281 5588 5654 7199 1132
Size: 97 1753 104 195 2307 66 1545 2229
Fragments arranged by size:
2307 2229 1753 1545 195 104 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8296
PciI A'CATG_T
Cuts at: 5584 7195 5584
Size: 1611 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8296
PmeI GTTT'AAAC
Cuts at: 5600 5600
Size: 8296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3940 3972 6365 6397 7878 7910 3940
Size: 32 2393 32 1481 32 4326
Fragments arranged by size:
4326 2393 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2060 1613
Size: 447 7849
PsiI TTA'TAA
Cuts at: 2786 5866 2786
Size: 3080 5216
PstI C_TGCA'G
Cuts at: 830 5648 830
Size: 4818 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8296
PvuII CAG'CTG
Cuts at: 2510 2753 3128 3457 4580 2510
Size: 243 375 329 1123 6226
Fragments arranged by size:
6226 1123 375 329 243
SacI G_AGCT'C
Cuts at: 4491 4491
Size: 8296
SalI G'TCGA_C
Cuts at: 1936 5638 1936
Size: 3702 4594
SapI GCTCTTCn'nnn_
Cuts at: 4479 4983 4479
Size: 504 7792
SbfI CC_TGCA'GG
Cuts at: 5648 5648
Size: 8296
ScaI AGT'ACT
Cuts at: 6544 8256 6544
Size: 1712 6584
SfcI C'TryA_G
Cuts at: 826 1045 2556 3334 5058 5502 5644 6779 7460
Size: 219 1511 778 1724 444 142 1135 681
Cuts at: 7460 7651 826
Size: 191 1471
Fragments arranged by size:
1724 1511 1471 1135 778 681 444 219
191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8296
SmaI CCC'GGG
Cuts at: 1872 5623 1872
Size: 3751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2960 4490 4617 6400 7301 7563
Size: 197 926 1017 1530 127 1783 901 262
Cuts at: 7563 820
Size: 1553
Fragments arranged by size:
1783 1553 1530 1017 926 901 262 197
127
SnaBI TAC'GTA
Cuts at: 493 6087 493
Size: 5594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8296
SphI G_CATG'C
Cuts at: 1132 3086 3281 5654 1132
Size: 1954 195 2373 3774
Fragments arranged by size:
3774 2373 1954 195
SspI AAT'ATT
Cuts at: 5 52 2348 5098 6220 5
Size: 47 2296 2750 1122 2081
Fragments arranged by size:
2750 2296 2081 1122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8296
StyI C'CwwG_G
Cuts at: 513 1065 3828 5358 5559 5735 513
Size: 552 2763 1530 201 176 3074
Fragments arranged by size:
3074 2763 1530 552 201 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 5587 6323 6340 6493 6678 7123
Size: 1255 492 3515 736 17 153 185 445
Cuts at: 7123 325
Size: 1498
Fragments arranged by size:
3515 1498 1255 736 492 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2383 6542 8254
Size: 274 80 33 51 1443 406 4159 1712
Cuts at: 8254 96
Size: 138
Fragments arranged by size:
4159 1712 1443 406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3715 3747 3954 3979 3986 4011 5571 5603 8256
Size: 32 207 25 7 25 1560 32 2653
Cuts at: 8256 8288 3715
Size: 32 3723
Fragments arranged by size:
3723 2653 1560 207 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8296
XbaI T'CTAG_A
Cuts at: 2026 3355 4096 5632 2026
Size: 1329 741 1536 4690
Fragments arranged by size:
4690 1536 1329 741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3549 3549
Size: 8296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8296
XmaI C'CCGG_G
Cuts at: 1870 5621 1870
Size: 3751 4545
XmnI GAAnn'nnTTC
Cuts at: 5539 6425 5539
Size: 886 7410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6104 276
Size: 53 83 186 936 4570 2468
Fragments arranged by size:
4570 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BsiWI BsmBI DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI