Restriction map for
pFN21AE9254
(Circular) (Six-base) MAPSORT of: pFN21AE9254.seq Check: 9804 from: 1 to: 6949
pFN21AE9254 6949 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2416 2569 2416
Size: 153 6796
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 3952 4292 1937
Size: 1890 125 340 4594
Fragments arranged by size:
4594 1890 340 125
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 2864 5076 5449 1563
Size: 1301 2212 373 3063
Fragments arranged by size:
3063 2212 1301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 5012 6396 6664 1632
Size: 1787 1593 1384 268 1917
Fragments arranged by size:
1917 1787 1593 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2737 820
Size: 197 1720 5032
Fragments arranged by size:
5032 1720 197
AflIII A'CryG_T
Cuts at: 1203 2530 3399 3989 4185 5848 6825 1203
Size: 1327 869 590 196 1663 977 1327
Fragments arranged by size:
1663 1327 1327 977 869 590 196
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 2147 1166
Size: 981 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3559 1184
Size: 2375 4574
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 6917
AlwNI CAG_nnn'CTG
Cuts at: 2059 2110 4692 6264 2059
Size: 51 2582 1572 2744
Fragments arranged by size:
2744 2582 1572 51
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 2196 3752 4258 4484 4636 6745 1493
Size: 703 1556 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 703 506 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 4274 1870
Size: 73 2331 4545
Fragments arranged by size:
4545 2331 73
AvrII C'CTAG_G
Cuts at: 3514 3514
Size: 6949
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2572 2605 2572
Size: 33 6916
BamHI G'GATC_C
Cuts at: 2650 4279 4646 2650
Size: 1629 367 4953
Fragments arranged by size:
4953 1629 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3543 4100 4270 5725 618
Size: 325 217 627 1756 557 170 1455 1842
Fragments arranged by size:
1842 1756 1455 627 557 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2963 3962 4076 4268 1391
Size: 1572 999 114 192 4072
Fragments arranged by size:
4072 1572 999 192 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2052 2215 928
Size: 971 153 163 5662
Fragments arranged by size:
5662 971 163 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3164 3198 5140 5174 1903
Size: 34 50 34 1143 34 1942 34 3678
Fragments arranged by size:
3678 1942 1143 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2573 4841 6051 2573
Size: 2268 1210 3471
Fragments arranged by size:
3471 2268 1210
BclI T'GATC_A
Cuts at: 1548 3319 1548
Size: 1771 5178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3438 5560 136
Size: 107 122 71 631 605 1766 2122 1525
Fragments arranged by size:
2122 1766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3524 6944 3524
Size: 3420 3529
BlpI GC'TnA_GC
Cuts at: 4366 4366
Size: 6949
Bme1580I G_kGCm'C
Cuts at: 1790 5010 6166 1790
Size: 3220 1156 2573
Fragments arranged by size:
3220 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 2370 4188 1318
Size: 723 329 1818 4079
Fragments arranged by size:
4079 1818 723 329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3965 4680 5638
Size: 416 53 83 399 24 2548 715 958
Cuts at: 5638 442
Size: 1753
Fragments arranged by size:
2548 1753 958 715 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2581 2872 3163 5609 1239
Size: 1342 291 291 2446 2579
Fragments arranged by size:
2579 2446 1342 291 291
Bpu10I CC'TnA_GC
Cuts at: 2069 2307 2401 2069
Size: 238 94 6617
Fragments arranged by size:
6617 238 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5074 5939 6237 5074
Size: 865 298 5786
Fragments arranged by size:
5786 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 4131 5612 882
Size: 617 1212 1420 1481 2219
Fragments arranged by size:
2219 1481 1420 1212 617
BsaAI yAC'GTr
Cuts at: 493 1204 2531 4740 493
Size: 711 1327 2209 2702
Fragments arranged by size:
2702 2209 1327 711
BsaBI GATnn'nnATC
Cuts at: 3308 6943 3308
Size: 3635 3314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2180 4081 4756 5138
Size: 53 83 186 936 647 1901 675 382
Cuts at: 5138 5773 275
Size: 635 1451
Fragments arranged by size:
1901 1451 936 675 647 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4076 5382 5780 6054 6201 6612 4076
Size: 1306 398 274 147 411 4413
Fragments arranged by size:
4413 1306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2690 2720 2870 2900 3161
Size: 30 165 30 1287 30 150 30 261
Cuts at: 3161 3191 1178
Size: 30 4936
Fragments arranged by size:
4936 1287 261 165 150 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2239 2908 2971 4191 1186
Size: 1053 669 63 1220 3944
Fragments arranged by size:
3944 1220 1053 669 63
BseYI C'CCAG_C
Cuts at: 2008 2617 3677 6152 6648 2008
Size: 609 1060 2475 496 2309
Fragments arranged by size:
2475 2309 1060 609 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 2523 2792 3961 1163
Size: 573 769 18 269 1169 4151
Fragments arranged by size:
4151 1169 769 573 269 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 5160 6188 1540
Size: 275 186 2398 761 1028 2301
Fragments arranged by size:
2398 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2834 2963 5010 5095 6166 2834
Size: 129 2047 85 1071 3617
Fragments arranged by size:
3617 2047 1071 129 85
BsmI GAATG_Cn'
Cuts at: 4460 4553 4460
Size: 93 6856
BsmBI CGTCTCn'nnnn_
Cuts at: 2171 2171
Size: 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2834 2963 3962 4076 4268 5010 5095
Size: 399 1044 129 999 114 192 742 85
Cuts at: 5095 6166 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1044 999 742 399 192 129
114 85
BspEI T'CCGG_A
Cuts at: 4076 6612 4076
Size: 2536 4413
BspHI T'CATG_A
Cuts at: 4838 6568 4838
Size: 1730 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2416 2495 2569 3527 4304 844
Size: 1572 79 74 958 777 3489
Fragments arranged by size:
3489 1572 958 777 79 74
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2779 5444 5618 65
Size: 1183 1531 2665 174 1396
Fragments arranged by size:
2665 1531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2820 5593 5780 1700
Size: 110 1010 2773 187 2869
Fragments arranged by size:
2869 2773 1010 187 110
BsrGI T'GTAC_A
Cuts at: 96 3402 96
Size: 3306 3643
BssHII G'CGCG_C
Cuts at: 1925 2020 1925
Size: 95 6854
BssSI C'ACGA_G
Cuts at: 3999 4009 4021 5009 6021 3999
Size: 10 12 988 1012 4927
Fragments arranged by size:
4927 1012 988 12 10
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6949
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 2612 1184
Size: 1428 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 2677
Size: 6949
BstYI r'GATC_y
Cuts at: 1972 2238 2650 3115 3524 4279 4646 5031 5048
Size: 266 412 465 409 755 367 385 17
Cuts at: 5048 6500 6586 6598 6944 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 755 465 412 409 385 367
346 266 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 2262 3280 3718 513
Size: 552 960 237 1018 438 3744
Fragments arranged by size:
3744 1018 960 552 438 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 4465 5259 5279 2418
Size: 2047 794 20 4088
Fragments arranged by size:
4088 2047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2172 2207 529
Size: 35 1608 35 5271
Fragments arranged by size:
5271 1608 35 35
DraI TTT'AAA
Cuts at: 3063 4253 3063
Size: 1190 5759
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4124 5956 809
Size: 507 2808 1832 1802
Fragments arranged by size:
2808 1832 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 4396 5285
Size: 54 1425 50 275 18 330 2236 889
Cuts at: 5285 6914 8
Size: 1629 43
Fragments arranged by size:
2236 1629 1425 889 330 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 4156 4164 4197 4879 1353
Size: 1023 1780 8 33 682 3423
Fragments arranged by size:
3423 1780 1023 682 33 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5536 5910 6056 387
Size: 1064 138 3947 374 146 1280
Fragments arranged by size:
3947 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2961 2961
Size: 6949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2581 2872 3163 3419 5012 5609 6396
Size: 393 949 291 291 256 1593 597 787
Cuts at: 6396 6664 1239
Size: 268 1524
Fragments arranged by size:
1593 1524 949 787 597 393 291 291
268 256
EcoNI CCTnn'n_nnAGG
Cuts at: 2997 2997
Size: 6949
EcoO109I rG'GnC_Cy
Cuts at: 2303 3413 2303
Size: 1110 5839
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
EcoRV GAT'ATC
Cuts at: 3857 3857
Size: 6949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 3098 3519 2989
Size: 109 421 6419
Fragments arranged by size:
6419 421 109
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 6096 1678
Size: 4418 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2690 2722 3087
Size: 32 135 32 782 32 304 32 365
Cuts at: 3087 3119 4015 4047 4172 4204 5590 5622 5664
Size: 32 896 32 125 32 1386 32 42
Cuts at: 5664 5696 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 896 782 365 304 135 125
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 4293 4539 669
Size: 988 281 1153 1202 246 3079
Fragments arranged by size:
3079 1202 1153 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3075 3761 748
Size: 2327 686 3936
Fragments arranged by size:
3936 2327 686
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 3969 4548 3969
Size: 579 6370
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3104 6063 6247 1305
Size: 1799 2959 184 2007
Fragments arranged by size:
2959 2007 1799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 10
Size: 54 1425 343 330 4797
Fragments arranged by size:
4797 1425 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2675 3226 3398 3988 4907 5266
Size: 648 981 528 551 172 590 919 359
Cuts at: 5266 5425 518
Size: 159 2042
Fragments arranged by size:
2042 981 919 648 590 551 528 359
172 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2107 2264 2542 4365 5042 6190 6435
Size: 450 51 157 278 1823 677 1148 245
Cuts at: 6435 1606
Size: 2120
Fragments arranged by size:
2120 1823 1148 677 450 278 245 157
51
NaeI GCC'GGC
Cuts at: 2822 2822
Size: 6949
NcoI C'CATG_G
Cuts at: 513 1065 2025 3280 513
Size: 552 960 1255 4182
Fragments arranged by size:
4182 1255 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6949
NgoMIV G'CCGG_C
Cuts at: 2820 2820
Size: 6949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 2104 3403 3993 4307 5852 1132
Size: 97 875 1299 590 314 1545 2229
Fragments arranged by size:
2229 1545 1299 875 590 314 97
PasI CC'CwG_GG
Cuts at: 1348 3934 4069 4096 1348
Size: 2586 135 27 4201
Fragments arranged by size:
4201 2586 135 27
PciI A'CATG_T
Cuts at: 3399 3989 5848 3399
Size: 590 1859 4500
Fragments arranged by size:
4500 1859 590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2646 1108
Size: 1538 5411
PfoI T'CCnGG_A
Cuts at: 2241 2888 3378 2241
Size: 647 490 5812
Fragments arranged by size:
5812 647 490
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2303 3413 2303
Size: 1110 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6949
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4175 4207 4175
Size: 32 6917
PstI C_TGCA'G
Cuts at: 830 2064 2296 2413 2588 4301 830
Size: 1234 232 117 175 1713 3478
Fragments arranged by size:
3478 1713 1234 232 175 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
PvuII CAG'CTG
Cuts at: 2056 2056
Size: 6949
SacI G_AGCT'C
Cuts at: 2963 2963
Size: 6949
SacII CC_GC'GG
Cuts at: 2265 2265
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SapI GCTCTTCn'nnn_
Cuts at: 4156 4156
Size: 6949
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SfcI C'TryA_G
Cuts at: 826 1045 2060 2292 2409 2584 3828 4297 5432
Size: 219 1015 232 117 175 1244 469 1135
Cuts at: 5432 6113 6304 826
Size: 681 191 1471
Fragments arranged by size:
1471 1244 1135 1015 681 469 232 219
191 175 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 4276 1872
Size: 2404 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2737 5053 5954 6216 820
Size: 197 926 794 2316 901 262 1553
Fragments arranged by size:
2316 1553 926 901 794 262 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 2104 4307 1132
Size: 972 2203 3774
Fragments arranged by size:
3774 2203 972
SspI AAT'ATT
Cuts at: 5 52 3338 3657 4873 5
Size: 47 3286 319 1216 2081
Fragments arranged by size:
3286 2081 1216 319 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6949
StyI C'CwwG_G
Cuts at: 513 1065 2025 2964 3280 3514 4388 513
Size: 552 960 939 316 234 874 3074
Fragments arranged by size:
3074 960 939 874 552 316 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3691 4088 4976 4993 5146 5331 5776
Size: 1255 2111 397 888 17 153 185 445
Cuts at: 5776 325
Size: 1498
Fragments arranged by size:
2111 1498 1255 888 445 397 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 3402 5195
Size: 274 80 33 51 1443 144 1281 1793
Cuts at: 5195 6907 96
Size: 1712 138
Fragments arranged by size:
1793 1712 1443 1281 274 144 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6909 6941 6909
Size: 32 6917
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 4285 4285
Size: 6949
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6949
XmaI C'CCGG_G
Cuts at: 1870 4274 1870
Size: 2404 4545
XmnI GAAnn'nnTTC
Cuts at: 3646 5078 3646
Size: 1432 5517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I DraIII FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI