Restriction map for
pFN21AE9253
(Circular) (Six-base) MAPSORT of: pFN21AE9253.seq Check: 4073 from: 1 to: 14047
pFN21AE9253 14047 bp
With 182 enzymes: SgfI *
February 3, 2015 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 10852 10852
Size: 14047
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11857 278
Size: 53 83 186 936 10321 2468
Fragments arranged by size:
10321 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 9580 11390 1937
Size: 1137 6506 1810 4594
Fragments arranged by size:
6506 4594 1810 1137
Acc65I G'GTAC_C
Cuts at: 7276 11368 7276
Size: 4092 9955
AclI AA'CG_TT
Cuts at: 1563 12174 12547 1563
Size: 10611 373 3063
Fragments arranged by size:
10611 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2114 3692 3977 4205 4413 5301 5660
Size: 407 75 1578 285 228 208 888 359
Cuts at: 5660 5855 7023 7215 7614 8036 8771 8996 9492
Size: 195 1168 192 399 422 735 225 496
Cuts at: 9492 10773 12110 13494 13762 1632
Size: 1281 1337 1384 268 1917
Fragments arranged by size:
1917 1578 1384 1337 1281 1168 888 735
496 422 407 399 359 285 268 228
225 208 195 192 75
AfeI AGC'GCT
Cuts at: 11067 11067
Size: 14047
AflII C'TTAA_G
Cuts at: 820 1017 3476 4711 4940 6952 7508 8459 9005
Size: 197 2459 1235 229 2012 556 951 546
Cuts at: 9005 820
Size: 5862
Fragments arranged by size:
5862 2459 2012 1235 951 556 546 229
197
AflIII A'CryG_T
Cuts at: 1203 4056 12946 13923 1203
Size: 2853 8890 977 1327
Fragments arranged by size:
8890 2853 1327 977
AgeI A'CCGG_T
Cuts at: 4254 12878 4254
Size: 8624 5423
AhdI GACnn_n'nnGTC
Cuts at: 2679 2766 3061 4069 5764 9138 9817 12776 2679
Size: 87 295 1008 1695 3374 679 2959 3950
Fragments arranged by size:
3950 3374 2959 1695 1008 679 295 87
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 14047
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6580 6611 1184
Size: 5396 31 8620
Fragments arranged by size:
8620 5396 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2946 2978 3387 3419 2946
Size: 32 409 32 13574
Fragments arranged by size:
13574 409 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4228 5299 6841 8143 9553 9961 10234 10493 11790
Size: 1071 1542 1302 1410 408 273 259 1297
Cuts at: 11790 13362 4228
Size: 1572 4913
Fragments arranged by size:
4913 1572 1542 1410 1302 1297 1071 408
273 259
ApaLI G'TGCA_C
Cuts at: 12104 13260 12104
Size: 1156 12891
ApoI r'AATT_y
Cuts at: 1493 2061 2296 2317 2445 3565 4474 4511 5152
Size: 568 235 21 128 1120 909 37 641
Cuts at: 5152 5255 5604 6107 6233 6261 6340 6744 7351
Size: 103 349 503 126 28 79 404 607
Cuts at: 7351 8539 9085 9247 9594 11356 11582 11734 13843
Size: 1188 546 162 347 1762 226 152 2109
Cuts at: 13843 1493
Size: 1697
Fragments arranged by size:
2109 1762 1697 1188 1120 909 641 607
568 546 503 404 349 347 235 226
162 152 128 126 103 79 37 28
21
AseI AT'TA_AT
Cuts at: 160 1032 2886 2990 3936 4950 12601 160
Size: 872 1854 104 946 1014 7651 1606
Fragments arranged by size:
7651 1854 1606 1014 946 872 104
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14047
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 6692 6755 10695 11372 1870
Size: 73 192 4557 63 3940 677 4545
Fragments arranged by size:
4557 4545 3940 677 192 73 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 10494 10527 10494
Size: 33 14014
BamHI G'GATC_C
Cuts at: 11377 11744 11377
Size: 367 13680
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7276 11180 11368 12823 618
Size: 325 217 627 5489 3904 188 1455 1842
Fragments arranged by size:
5489 3904 1842 1455 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2053 3204 6293 6420 6499 6756 7229 8330
Size: 662 1151 3089 127 79 257 473 1101
Cuts at: 8330 8929 9354 10005 10272 11168 11366 1391
Size: 599 425 651 267 896 198 4072
Fragments arranged by size:
4072 3089 1151 1101 896 662 651 599
473 425 267 257 198 127 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 3965 4181 4680 4824 5492 6059
Size: 971 779 1287 216 499 144 668 567
Cuts at: 6059 6096 10917 928
Size: 37 4821 4058
Fragments arranged by size:
4821 4058 1287 971 779 668 567 499
216 144 37
BbvCI CC'TCA_GC
Cuts at: 3434 3434
Size: 14047
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 12238 12272 1903
Size: 34 10301 34 3678
Fragments arranged by size:
10301 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10495 11939 13149 10495
Size: 1444 1210 11393
Fragments arranged by size:
11393 1444 1210
BclI T'GATC_A
Cuts at: 1548 4366 5050 6271 7558 10398 1548
Size: 2818 684 1221 1287 2840 5197
Fragments arranged by size:
5197 2840 2818 1287 1221 684
BfrBI ATG'CAT
Cuts at: 4384 4624 4955 5108 7239 7479 7621 9880 4384
Size: 240 331 153 2131 240 142 2259 8551
Fragments arranged by size:
8551 2259 2131 331 240 240 153 142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 8285 9398
Size: 107 122 71 631 605 898 5715 1113
Cuts at: 9398 12658 136
Size: 3260 1525
Fragments arranged by size:
5715 3260 1525 1113 898 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2500 3619 4204 5336 5734 14042 2500
Size: 1119 585 1132 398 8308 2505
Fragments arranged by size:
8308 2505 1132 1119 585 398
BlpI GC'TnA_GC
Cuts at: 7098 8324 11157 11464 7098
Size: 1226 2833 307 9681
Fragments arranged by size:
9681 2833 1226 307
Bme1580I G_kGCm'C
Cuts at: 1790 4720 10378 12108 13264 1790
Size: 2930 5658 1730 1156 2573
Fragments arranged by size:
5658 2930 2573 1730 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 14047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4200 11162 11210
Size: 416 53 83 399 24 2783 6962 48
Cuts at: 11210 11778 12736 442
Size: 568 958 1753
Fragments arranged by size:
6962 2783 1753 958 568 416 399 83
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 7743 9944 1056
Size: 6687 2201 5159
Fragments arranged by size:
6687 5159 2201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 14047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2990 3539 4028 6878 6917 7188 7559
Size: 1022 729 549 489 2850 39 271 371
Cuts at: 7559 7787 8087 9575 9966 10256 11333 12707 1239
Size: 228 300 1488 391 290 1077 1374 2579
Fragments arranged by size:
2850 2579 1488 1374 1077 1022 729 549
489 391 371 300 290 271 228 39
Bpu10I CC'TnA_GC
Cuts at: 3434 4856 3434
Size: 1422 12625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3515 3699 4742 6680 6932 7997 9095 9464 12172
Size: 184 1043 1938 252 1065 1098 369 2708
Cuts at: 12172 13037 13335 3515
Size: 865 298 4227
Fragments arranged by size:
4227 2708 1938 1098 1065 1043 865 369
298 252 184
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 9098 10707 12710 882
Size: 617 2580 5019 1609 2003 2219
Fragments arranged by size:
5019 2580 2219 2003 1609 617
BsaAI yAC'GTr
Cuts at: 493 1204 7640 11838 493
Size: 711 6436 4198 2702
Fragments arranged by size:
6436 4198 2702 711
BsaBI GATnn'nnATC
Cuts at: 7074 8750 14041 7074
Size: 1676 5291 7080
Fragments arranged by size:
7080 5291 1676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3062 11854 12236 12871
Size: 53 83 186 936 1529 8792 382 635
Cuts at: 12871 275
Size: 1451
Fragments arranged by size:
8792 1529 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4254 5099 12480 12878 13152 13299 13710 4254
Size: 845 7381 398 274 147 411 4591
Fragments arranged by size:
7381 4591 845 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2946 2976 4429 4459 5684
Size: 30 165 30 1543 30 1453 30 1225
Cuts at: 5684 5714 6851 6857 6881 6887 7644 7674 1178
Size: 30 1137 6 24 6 757 30 7551
Fragments arranged by size:
7551 1543 1453 1225 1137 757 165 30
30 30 30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3212 3451 4450 5745 6425 6472 6617 1186
Size: 2026 239 999 1295 680 47 145 8616
Fragments arranged by size:
8616 2026 1295 999 680 239 145 47
BseYI C'CCAG_C
Cuts at: 2010 6400 6637 11294 13250 13746 2010
Size: 4390 237 4657 1956 496 2311
Fragments arranged by size:
4657 4390 2311 1956 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3406 5342 6586 7164 8396 10452 11037
Size: 573 1670 1936 1244 578 1232 2056 585
Cuts at: 11037 1163
Size: 4173
Fragments arranged by size:
4173 2056 1936 1670 1244 1232 585 578
573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 11497 12258 13286 1540
Size: 275 186 9496 761 1028 2301
Fragments arranged by size:
9496 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 3204 5879 6180 6293 6499 6756 7169 8330
Size: 1151 2675 301 113 206 257 413 1161
Cuts at: 8330 9354 9648 10005 10272 12108 12193 13264 2053
Size: 1024 294 357 267 1836 85 1071 2836
Fragments arranged by size:
2836 2675 1836 1161 1151 1071 1024 413
357 301 294 267 257 206 113 85
BsmI GAATG_Cn'
Cuts at: 5171 5186 7241 8007 11558 11651 5171
Size: 15 2055 766 3551 93 7567
Fragments arranged by size:
7567 3551 2055 766 93 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 3204 4720 5879 6180 6293 6420
Size: 399 263 1151 1516 1159 301 113 127
Cuts at: 6420 6499 6756 7169 7229 8330 8929 9354 9648
Size: 79 257 413 60 1101 599 425 294
Cuts at: 9648 10005 10272 10378 11168 11366 12108 12193 13264
Size: 357 267 106 790 198 742 85 1071
Cuts at: 13264 1391
Size: 2174
Fragments arranged by size:
2174 1516 1159 1151 1101 1071 790 742
599 425 413 399 357 301 294 267
263 257 198 127 113 106 85 79
60
BspEI T'CCGG_A
Cuts at: 13710 13710
Size: 14047
BspHI T'CATG_A
Cuts at: 2266 3979 4605 5400 5833 10114 10887 11936 13666
Size: 1713 626 795 433 4281 773 1049 1730
Cuts at: 13666 2266
Size: 2647
Fragments arranged by size:
4281 2647 1730 1713 1049 795 773 626
433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4557 10852 11402 844
Size: 3713 6295 550 3489
Fragments arranged by size:
6295 3713 3489 550
BsrBI CCG'CTC
Cuts at: 1394 1823 8443 11934 1394
Size: 429 6620 3491 3507
Fragments arranged by size:
6620 3507 3491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 8002 12542 12716 65
Size: 1183 1374 5380 4540 174 1396
Fragments arranged by size:
5380 4540 1396 1374 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4254 6681 12691 12878 1700
Size: 110 2444 2427 6010 187 2869
Fragments arranged by size:
6010 2869 2444 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 14047
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 14047
BssSI C'ACGA_G
Cuts at: 12107 13119 12107
Size: 1012 13035
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3143 5635 5761 794
Size: 2349 2492 126 9080
Fragments arranged by size:
9080 2492 2349 126
BstBI TT'CG_AA
Cuts at: 12851 12851
Size: 14047
BstEII G'GTnAC_C
Cuts at: 1184 8834 1184
Size: 7650 6397
BstXI CCAn_nnnn'nTGG
Cuts at: 4931 7895 7933 8281 4931
Size: 2964 38 348 10697
Fragments arranged by size:
10697 2964 348 38
BstYI r'GATC_y
Cuts at: 1972 2500 3619 4204 4297 5116 5336 5587 5734
Size: 528 1119 585 93 819 220 251 147
Cuts at: 5734 9708 10805 11377 11744 12129 12146 13598 13684
Size: 3974 1097 572 367 385 17 1452 86
Cuts at: 13684 13696 14042 1972
Size: 12 346 1977
Fragments arranged by size:
3974 1977 1452 1119 1097 819 585 572
528 385 367 346 251 220 147 93
86 17 12
BstZ17I GTA'TAC
Cuts at: 3075 3075
Size: 14047
Bsu36I CC'TnA_GG
Cuts at: 4292 6797 11267 4292
Size: 2505 4470 7072
Fragments arranged by size:
7072 4470 2505
BtgI C'CryG_G
Cuts at: 513 1065 6073 7926 9321 11022 513
Size: 552 5008 1853 1395 1701 3538
Fragments arranged by size:
5008 3538 1853 1701 1395 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 8401 487
Size: 683 56 7175 6133
Fragments arranged by size:
7175 6133 683 56
BtsI GCAGTG_nn'
Cuts at: 4565 6574 7152 8781 9315 11332 11563 12357 12377
Size: 2009 578 1629 534 2017 231 794 20
Cuts at: 12377 4565
Size: 6235
Fragments arranged by size:
6235 2017 2009 1629 794 578 534 231
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 14047
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 14012
DraI TTT'AAA
Cuts at: 3552 4752 4896 5388 5409 5575 8713 10251 11351
Size: 1200 144 492 21 166 3138 1538 1100
Cuts at: 11351 3552
Size: 6248
Fragments arranged by size:
6248 3138 1538 1200 1100 492 166 144
21
DraIII CAC_nnn'GTG
Cuts at: 4057 6139 4057
Size: 2082 11965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 13054 809
Size: 507 11738 1802
Fragments arranged by size:
11738 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 7662 8586 10774
Size: 54 1425 50 275 18 5832 924 2188
Cuts at: 10774 11494 12383 14012 8
Size: 720 889 1629 43
Fragments arranged by size:
5832 2188 1629 1425 924 889 720 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 11494 1537
Size: 275 9682 4090
Fragments arranged by size:
9682 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2598 3360 3840 4560 4908 6009 6648
Size: 705 540 762 480 720 348 1101 639
Cuts at: 6648 6699 7234 8353 8574 8710 8883 10025 10260
Size: 51 535 1119 221 136 173 1142 235
Cuts at: 10260 10416 11977 1353
Size: 156 1561 3423
Fragments arranged by size:
3423 1561 1142 1119 1101 762 720 705
639 540 535 480 348 235 221 173
156 136 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 12634 13008 13154 387
Size: 1064 138 11045 374 146 1280
Fragments arranged by size:
11045 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2051 3202 6291 6497 6754 8328 9352 10003 10270
Size: 1151 3089 206 257 1574 1024 651 267
Cuts at: 10270 2051
Size: 5828
Fragments arranged by size:
5828 3089 1574 1151 1024 651 267 257
206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2114 2261 2990 3539 3692 3977
Size: 393 407 75 147 729 549 153 285
Cuts at: 3977 4028 4205 4413 5301 5660 5855 6878 6917
Size: 51 177 208 888 359 195 1023 39
Cuts at: 6917 7023 7188 7215 7559 7614 7787 8036 8087
Size: 106 165 27 344 55 173 249 51
Cuts at: 8087 8771 8996 9492 9575 9966 10256 10773 11333
Size: 684 225 496 83 391 290 517 560
Cuts at: 11333 12110 12707 13494 13762 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 1023 888 787 777 729 684 597
560 549 517 496 407 393 391 359
344 290 285 268 249 225 208 195
177 173 165 153 147 106 83 75
55 51 51 39 27
EcoNI CCTnn'n_nnAGG
Cuts at: 6671 9473 10710 6671
Size: 2802 1237 10008
Fragments arranged by size:
10008 2802 1237
EcoO109I rG'GnC_Cy
Cuts at: 5828 7105 11263 5828
Size: 1277 4158 8612
Fragments arranged by size:
8612 4158 1277
EcoRI G'AATT_C
Cuts at: 2317 5152 5255 8539 9085 11356 11734 2317
Size: 2835 103 3284 546 2271 378 4630
Fragments arranged by size:
4630 3284 2835 2271 546 378 103
EcoRV GAT'ATC
Cuts at: 7309 7309
Size: 14047
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2099 3937 4443 4996 5087 8222 9629 10568 11114
Size: 1838 506 553 91 3135 1407 939 546
Cuts at: 11114 2099
Size: 5032
Fragments arranged by size:
5032 3135 1838 1407 939 553 546 506
91
FspI TGC'GCA
Cuts at: 2400 5628 12553 2400
Size: 3228 6925 3894
Fragments arranged by size:
6925 3894 3228
HaeII r_GCGC'y
Cuts at: 1678 11069 13194 1678
Size: 9391 2125 2531
Fragments arranged by size:
9391 2531 2125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2665 2697 2752 2784 3047
Size: 32 135 32 1093 32 55 32 263
Cuts at: 3047 3079 3770 3802 4055 4087 4429 4461 4792
Size: 32 691 32 253 32 342 32 331
Cuts at: 4792 4824 5750 5782 5963 5995 6647 6679 6851
Size: 32 926 32 181 32 652 32 172
Cuts at: 6851 6883 7619 7651 7898 7928 7930 7960 7964
Size: 32 736 32 247 30 2 30 4
Cuts at: 7964 7996 9124 9156 9212 9244 9803 9835 10318
Size: 32 1128 32 56 32 559 32 483
Cuts at: 10318 10350 12688 12720 12762 12794 1373
Size: 32 2338 32 42 32 2626
Fragments arranged by size:
2626 2338 1128 1093 926 736 691 652
559 483 342 331 263 253 247 181
172 135 56 55 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3212 5844 6322 6354 11391
Size: 988 281 1128 146 2632 478 32 5037
Cuts at: 11391 11637 669
Size: 246 3079
Fragments arranged by size:
5037 3079 2632 1128 988 478 281 246
146 32
HindIII A'AGCT_T
Cuts at: 748 3674 4217 4858 10785 748
Size: 2926 543 641 5927 4010
Fragments arranged by size:
5927 4010 2926 641 543
HpaI GTT'AAC
Cuts at: 11637 11637
Size: 14047
KpnI G_GTAC'C
Cuts at: 7280 11372 7280
Size: 4092 9955
MfeI C'AATT_G
Cuts at: 9992 11646 9992
Size: 1654 12393
MluI A'CGCG_T
Cuts at: 13923 13923
Size: 14047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2874 2983 2989 3848 5196 5292 9945 11267
Size: 1569 109 6 859 1348 96 4653 1322
Cuts at: 11267 11343 13161 13345 1305
Size: 76 1818 184 2007
Fragments arranged by size:
4653 2007 1818 1569 1348 1322 859 184
109 96 76 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7664 8588 10776 10
Size: 54 1425 343 5832 924 2188 3281
Fragments arranged by size:
5832 3281 2188 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 4692 7989 8012 9320 9326 12005
Size: 648 2085 1441 3297 23 1308 6 2679
Cuts at: 12005 12364 12523 518
Size: 359 159 2042
Fragments arranged by size:
3297 2679 2085 2042 1441 1308 648 359
159 23 6
MspA1I CmG'CkG
Cuts at: 1606 5470 6400 7680 8353 8410 8899 10417 11123
Size: 3864 930 1280 673 57 489 1518 706
Cuts at: 11123 11161 11463 12140 13288 13533 1606
Size: 38 302 677 1148 245 2120
Fragments arranged by size:
3864 2120 1518 1280 1148 930 706 677
673 489 302 245 57 38
NcoI C'CATG_G
Cuts at: 513 1065 6073 9321 11022 513
Size: 552 5008 3248 1701 3538
Fragments arranged by size:
5008 3538 3248 1701 552
NdeI CA'TA_TG
Cuts at: 387 5110 387
Size: 4723 9324
NheI G'CTAG_C
Cuts at: 1052 7739 9940 1052
Size: 6687 2201 5159
Fragments arranged by size:
6687 5159 2201
NotI GC'GGCC_GC
Cuts at: 11494 11494
Size: 14047
NsiI A_TGCA'T
Cuts at: 4386 4626 4957 5110 7241 7481 7623 9882 4386
Size: 240 331 153 2131 240 142 2259 8551
Fragments arranged by size:
8551 2259 2131 331 240 240 153 142
NspI r_CATG'y
Cuts at: 1132 1229 4277 4426 5764 7479 7625 9068 10048
Size: 97 3048 149 1338 1715 146 1443 980
Cuts at: 10048 11405 12950 1132
Size: 1357 1545 2229
Fragments arranged by size:
3048 2229 1715 1545 1443 1357 1338 980
149 146 97
PasI CC'CwG_GG
Cuts at: 1348 7222 10378 1348
Size: 5874 3156 5017
Fragments arranged by size:
5874 5017 3156
PciI A'CATG_T
Cuts at: 12946 12946
Size: 14047
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3232 11123 1108
Size: 902 1222 7891 4032
Fragments arranged by size:
7891 4032 1222 902
PfoI T'CCnGG_A
Cuts at: 2301 8103 10726 2301
Size: 5802 2623 5622
Fragments arranged by size:
5802 5622 2623
PmeI GTTT'AAAC
Cuts at: 11351 11351
Size: 14047
PmlI CAC'GTG
Cuts at: 7640 7640
Size: 14047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 2978 12116 12148 13629 13661 2946
Size: 32 9138 32 1481 32 3332
Fragments arranged by size:
9138 3332 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 7105 7105
Size: 14047
PshAI GACnn'nnGTC
Cuts at: 1613 4464 6063 11314 1613
Size: 2851 1599 5251 4346
Fragments arranged by size:
5251 4346 2851 1599
PsiI TTA'TAA
Cuts at: 5622 10610 11617 5622
Size: 4988 1007 8052
Fragments arranged by size:
8052 4988 1007
PspXI vC'TCGA_Gb
Cuts at: 6755 6755
Size: 14047
PstI C_TGCA'G
Cuts at: 830 2151 2410 3312 5709 5937 6312 6600 8118
Size: 1321 259 902 2397 228 375 288 1518
Cuts at: 8118 8232 8776 9765 9870 10443 10909 11399 830
Size: 114 544 989 105 573 466 490 3478
Fragments arranged by size:
3478 2397 1518 1321 989 902 573 544
490 466 375 288 259 228 114 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14047
PvuII CAG'CTG
Cuts at: 5470 6400 7680 8353 8410 8899 10417 11123 5470
Size: 930 1280 673 57 489 1518 706 8394
Fragments arranged by size:
8394 1518 1280 930 706 673 489 57
SacI G_AGCT'C
Cuts at: 2053 3204 6293 6499 6756 8330 9354 10005 10272
Size: 1151 3089 206 257 1574 1024 651 267
Cuts at: 10272 2053
Size: 5828
Fragments arranged by size:
5828 3089 1574 1151 1024 651 267 257
206
SalI G'TCGA_C
Cuts at: 1936 11389 1936
Size: 9453 4594
SapI GCTCTTCn'nnn_
Cuts at: 2058 4908 6648 7234 8883 10025 10260 2058
Size: 2850 1740 586 1649 1142 235 5845
Fragments arranged by size:
5845 2850 1740 1649 1142 586 235
SbfI CC_TGCA'GG
Cuts at: 3312 8232 11399 3312
Size: 4920 3167 5960
Fragments arranged by size:
5960 4920 3167
ScaI AGT'ACT
Cuts at: 10595 12295 14007 10595
Size: 1700 1712 10635
Fragments arranged by size:
10635 1712 1700
SexAI A'CCwGG_T
Cuts at: 10056 10056
Size: 14047
SfcI C'TryA_G
Cuts at: 826 1045 2147 2406 3308 4112 5705 5933 6308
Size: 219 1102 259 902 804 1593 228 375
Cuts at: 6308 6596 7047 8114 8228 8772 9761 9866 10439
Size: 288 451 1067 114 544 989 105 573
Cuts at: 10439 10905 11395 12530 13211 13402 826
Size: 466 490 1135 681 191 1471
Fragments arranged by size:
1593 1471 1135 1102 1067 989 902 804
681 573 544 490 466 451 375 288
259 228 219 191 114 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 8285 8285
Size: 14047
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14047
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14047
SmaI CCC'GGG
Cuts at: 1872 11374 1872
Size: 9502 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3476 3530 3714 4711 4721
Size: 197 926 192 1341 54 184 997 10
Cuts at: 4721 4940 6659 6692 6755 6911 6952 7508 7976
Size: 219 1719 33 63 156 41 556 468
Cuts at: 7976 8459 9005 9074 9443 12151 13052 13314 820
Size: 483 546 69 369 2708 901 262 1553
Fragments arranged by size:
2708 1719 1553 1341 997 926 901 556
546 483 468 369 262 219 197 192
184 156 69 63 54 41 33 10
SnaBI TAC'GTA
Cuts at: 493 11838 493
Size: 11345 2702
SpeI A'CTAG_T
Cuts at: 152 3031 5186 152
Size: 2879 2155 9013
Fragments arranged by size:
9013 2879 2155
SphI G_CATG'C
Cuts at: 1132 4277 4426 7479 7625 9068 10048 11405 1132
Size: 3145 149 3053 146 1443 980 1357 3774
Fragments arranged by size:
3774 3145 3053 1443 1357 980 149 146
SspI AAT'ATT
Cuts at: 5 52 2477 6048 7547 9265 11971 5
Size: 47 2425 3571 1499 1718 2706 2081
Fragments arranged by size:
3571 2706 2425 2081 1718 1499 47
StuI AGG'CCT
Cuts at: 1517 7599 8279 9468 1517
Size: 6082 680 1189 6096
Fragments arranged by size:
6096 6082 1189 680
StyI C'CwwG_G
Cuts at: 513 1065 6073 7665 8274 8280 9321 10506 10566
Size: 552 5008 1592 609 6 1041 1185 60
Cuts at: 10566 11022 11486 513
Size: 456 464 3074
Fragments arranged by size:
5008 3074 1592 1185 1041 609 552 464
456 60 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 6951 12074 12091 12244 12429 12874
Size: 1255 1704 3667 5123 17 153 185 445
Cuts at: 12874 325
Size: 1498
Fragments arranged by size:
5123 3667 1704 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5055 9292 10593
Size: 274 80 33 51 1443 3078 4237 1301
Cuts at: 10593 12293 14005 96
Size: 1700 1712 138
Fragments arranged by size:
4237 3078 1712 1700 1443 1301 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 5684 5716 7644 7676 14007 14039 3227
Size: 32 2425 32 1928 32 6331 32 3235
Fragments arranged by size:
6331 3235 2425 1928 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6187 8059 8618 1316
Size: 4871 1872 559 6745
Fragments arranged by size:
6745 4871 1872 559
XbaI T'CTAG_A
Cuts at: 4207 5509 5611 7114 8869 9781 11383 4207
Size: 1302 102 1503 1755 912 1602 6871
Fragments arranged by size:
6871 1755 1602 1503 1302 912 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3154 11044 11308 3154
Size: 7890 264 5893
Fragments arranged by size:
7890 5893 264
XhoI C'TCGA_G
Cuts at: 1943 2135 6692 6755 1943
Size: 192 4557 63 9235
Fragments arranged by size:
9235 4557 192 63
XmaI C'CCGG_G
Cuts at: 1870 11372 1870
Size: 9502 4545
XmnI GAAnn'nnTTC
Cuts at: 8049 12176 8049
Size: 4127 9920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11855 276
Size: 53 83 186 936 10321 2468
Fragments arranged by size:
10321 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BsiWI BsmBI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI
RsrII SacII SanDI SfoI SrfI SwaI