Restriction map for
pFN21AE9249
(Circular) (Six-base) MAPSORT of: pFN21AE9249.seq Check: 7563 from: 1 to: 5125
pFN21AE9249 5125 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2935 278
Size: 53 83 186 936 1399 2468
Fragments arranged by size:
2468 1399 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2468 1937
Size: 531 4594
Acc65I G'GTAC_C
Cuts at: 2446 2446
Size: 5125
AclI AA'CG_TT
Cuts at: 1563 3252 3625 1563
Size: 1689 373 3063
Fragments arranged by size:
3063 1689 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2150 2188 3188 4572 4840 1632
Size: 518 38 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1000 518 268 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4928
AflIII A'CryG_T
Cuts at: 1203 4024 5001 1203
Size: 2821 977 1327
Fragments arranged by size:
2821 1327 977
AgeI A'CCGG_T
Cuts at: 3956 3956
Size: 5125
AhdI GACnn_n'nnGTC
Cuts at: 3854 3854
Size: 5125
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5125
AlwNI CAG_nnn'CTG
Cuts at: 2868 4440 2868
Size: 1572 3553
ApaI G_GGCC'C
Cuts at: 2012 2096 2012
Size: 84 5041
ApaLI G'TGCA_C
Cuts at: 3182 4338 3182
Size: 1156 3969
ApoI r'AATT_y
Cuts at: 1493 2234 2434 2660 2812 4921 1493
Size: 741 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 741 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 3679 160
Size: 872 2647 1606
Fragments arranged by size:
2647 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5125
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2041 2056 2450 1870
Size: 73 90 8 15 394 4545
Fragments arranged by size:
4545 394 90 73 15 8
BamHI G'GATC_C
Cuts at: 2397 2455 2822 2397
Size: 58 367 4700
Fragments arranged by size:
4700 367 58
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2446 3901 618
Size: 325 217 627 659 1455 1842
Fragments arranged by size:
1842 1455 659 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2063 2096 2444 1391
Size: 621 51 33 348 4072
Fragments arranged by size:
4072 621 348 51 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3316 3350 1903
Size: 34 1379 34 3678
Fragments arranged by size:
3678 1379 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3017 4227 3017
Size: 1210 3915
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2279 3736 136
Size: 107 122 71 631 605 607 1457 1525
Fragments arranged by size:
1525 1457 631 607 605 122 107 71
BglII A'GATC_T
Cuts at: 5120 5120
Size: 5125
BlpI GC'TnA_GC
Cuts at: 2542 2542
Size: 5125
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2096 3186 4342 1790
Size: 222 84 1090 1156 2573
Fragments arranged by size:
2573 1156 1090 222 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3814 442
Size: 416 53 83 399 24 1439 958 1753
Fragments arranged by size:
1753 1439 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2381 2381
Size: 5125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3785 1239
Size: 2546 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 3250 4115 4413 2429
Size: 821 865 298 3141
Fragments arranged by size:
3141 865 821 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3788 882
Size: 617 2289 2219
Fragments arranged by size:
2289 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2916 493
Size: 711 1712 2702
Fragments arranged by size:
2702 1712 711
BsaBI GATnn'nnATC
Cuts at: 5119 5119
Size: 5125
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2328 2932 3314 3949
Size: 53 83 186 936 795 604 382 635
Cuts at: 3949 275
Size: 1451
Fragments arranged by size:
1451 936 795 635 604 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3558 3956 4230 4377 4788 3558
Size: 398 274 147 411 3895
Fragments arranged by size:
3895 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 1178
Size: 30 165 30 722 30 4148
Fragments arranged by size:
4148 722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2327 2426 1186
Size: 873 268 99 3885
Fragments arranged by size:
3885 873 268 99
BseYI C'CCAG_C
Cuts at: 4328 4824 4328
Size: 496 4629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4552
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 3336 4364 1540
Size: 275 186 574 761 1028 2301
Fragments arranged by size:
2301 1028 761 574 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 3186 3271 4342 2177
Size: 1009 85 1071 2960
Fragments arranged by size:
2960 1071 1009 85
BsmI GAATG_Cn'
Cuts at: 2636 2729 2636
Size: 93 5032
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2063 2096 2177 2444 3186 3271
Size: 399 222 51 33 81 267 742 85
Cuts at: 3271 4342 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 222 85 81
51 33
BspEI T'CCGG_A
Cuts at: 4788 4788
Size: 5125
BspHI T'CATG_A
Cuts at: 3014 4744 3014
Size: 1730 3395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2480 844
Size: 1636 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3012 1394
Size: 429 1189 3507
Fragments arranged by size:
3507 1189 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3620 3794 65
Size: 1183 2372 174 1396
Fragments arranged by size:
2372 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3769 3956 1700
Size: 110 1959 187 2869
Fragments arranged by size:
2869 1959 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5125
BssSI C'ACGA_G
Cuts at: 2241 3185 4197 2241
Size: 944 1012 3169
Fragments arranged by size:
3169 1012 944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5125
BstBI TT'CG_AA
Cuts at: 3929 3929
Size: 5125
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5125
BstYI r'GATC_y
Cuts at: 1972 2154 2397 2455 2822 3207 3224 4676 4762
Size: 182 243 58 367 385 17 1452 86
Cuts at: 4762 4774 5120 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 243 182 86
58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2101 2101
Size: 5125
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3634
Fragments arranged by size:
3634 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4386
Fragments arranged by size:
4386 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3435 3455 2641
Size: 794 20 4311
Fragments arranged by size:
4311 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5090
DraI TTT'AAA
Cuts at: 2429 2429
Size: 5125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4132 809
Size: 507 2816 1802
Fragments arranged by size:
2816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2572 3461 5090
Size: 54 1425 50 275 18 742 889 1629
Cuts at: 5090 8
Size: 43
Fragments arranged by size:
1629 1425 889 742 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2572 1537
Size: 275 760 4090
Fragments arranged by size:
4090 760 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2372 3055 1353
Size: 812 207 683 3423
Fragments arranged by size:
3423 812 683 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2146 3712 4086 4232 387
Size: 1064 138 557 1566 374 146 1280
Fragments arranged by size:
1566 1280 1064 557 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2188 3188 3785 4572 4840 1239
Size: 393 518 38 1000 597 787 268 1524
Fragments arranged by size:
1524 1000 787 597 518 393 268 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2411 2288
Size: 123 5002
EcoO109I rG'GnC_Cy
Cuts at: 2038 2092 2093 2272 2404 2038
Size: 54 1 179 132 4759
Fragments arranged by size:
4759 179 132 54 1
EcoRI G'AATT_C
Cuts at: 2434 2812 2434
Size: 378 4747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 5125
FspI TGC'GCA
Cuts at: 3631 3631
Size: 5125
HaeII r_GCGC'y
Cuts at: 1678 2109 4272 1678
Size: 431 2163 2531
Fragments arranged by size:
2531 2163 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3766 3798 3840 3872 1373
Size: 32 135 32 2194 32 42 32 2626
Fragments arranged by size:
2626 2194 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2469 2715 669
Size: 988 281 531 246 3079
Fragments arranged by size:
3079 988 531 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 3711
HpaI GTT'AAC
Cuts at: 2715 2715
Size: 5125
KpnI G_GTAC'C
Cuts at: 2450 2450
Size: 5125
MfeI C'AATT_G
Cuts at: 2257 2724 2257
Size: 467 4658
MluI A'CGCG_T
Cuts at: 5001 5001
Size: 5125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4239 4423 1305
Size: 2934 184 2007
Fragments arranged by size:
2934 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3303
Fragments arranged by size:
3303 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3442 3601 518
Size: 648 1917 359 159 2042
Fragments arranged by size:
2042 1917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2541 3218 4366 4611 1606
Size: 935 677 1148 245 2120
Fragments arranged by size:
2120 1148 935 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3634
Fragments arranged by size:
3634 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5125
NotI GC'GGCC_GC
Cuts at: 2572 2572
Size: 5125
NspI r_CATG'y
Cuts at: 1132 1229 2351 2483 4028 1132
Size: 97 1122 132 1545 2229
Fragments arranged by size:
2229 1545 1122 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5125
PciI A'CATG_T
Cuts at: 4024 4024
Size: 5125
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5125
PmeI GTTT'AAAC
Cuts at: 2429 2429
Size: 5125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 4707 4739 3194
Size: 32 1481 32 3580
Fragments arranged by size:
3580 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5125
PsiI TTA'TAA
Cuts at: 2695 2695
Size: 5125
PspOMI G'GGCC_C
Cuts at: 2008 2092 2008
Size: 84 5041
PstI C_TGCA'G
Cuts at: 830 2477 830
Size: 1647 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5125
SalI G'TCGA_C
Cuts at: 1936 2467 1936
Size: 531 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 2165
Size: 5125
SbfI CC_TGCA'GG
Cuts at: 2477 2477
Size: 5125
ScaI AGT'ACT
Cuts at: 3373 5085 3373
Size: 1712 3413
SfcI C'TryA_G
Cuts at: 826 1045 2473 3608 4289 4480 826
Size: 219 1428 1135 681 191 1471
Fragments arranged by size:
1471 1428 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2279 2279
Size: 5125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5125
SmaI CCC'GGG
Cuts at: 1872 2452 1872
Size: 580 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 3229 4130 4392 820
Size: 197 926 465 821 901 262 1553
Fragments arranged by size:
1553 926 901 821 465 262 197
SnaBI TAC'GTA
Cuts at: 493 2916 493
Size: 2423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5125
SphI G_CATG'C
Cuts at: 1132 2483 1132
Size: 1351 3774
SspI AAT'ATT
Cuts at: 5 52 3049 5
Size: 47 2997 2081
Fragments arranged by size:
2997 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2564 513
Size: 552 939 560 3074
Fragments arranged by size:
3074 939 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3152 3169 3322 3507 3952 325
Size: 1255 1572 17 153 185 445 1498
Fragments arranged by size:
1572 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3371 5083 96
Size: 274 80 33 51 1443 1394 1712 138
Fragments arranged by size:
1712 1443 1394 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5085 5117 5085
Size: 32 5093
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5125
XbaI T'CTAG_A
Cuts at: 2461 2461
Size: 5125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5125
XmaI C'CCGG_G
Cuts at: 1870 2450 1870
Size: 580 4545
XmnI GAAnn'nnTTC
Cuts at: 2304 3254 2304
Size: 950 4175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2933 276
Size: 53 83 186 936 1399 2468
Fragments arranged by size:
2468 1399 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI
XcmI