Restriction map for
pFN21AE9246
(Circular) (Six-base) MAPSORT of: pFN21AE9246.seq Check: 2066 from: 1 to: 9715
pFN21AE9246 9715 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5685 7525 278
Size: 53 83 186 936 4149 1840 2468
Fragments arranged by size:
4149 2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4702 7058 1937
Size: 2765 2356 4594
Fragments arranged by size:
4594 2765 2356
Acc65I G'GTAC_C
Cuts at: 7036 7036
Size: 9715
AclI AA'CG_TT
Cuts at: 1563 7842 8215 1563
Size: 6279 373 3063
Fragments arranged by size:
6279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 3852 3875 5496 5667 6263 6402 6729
Size: 893 1327 23 1621 171 596 139 327
Cuts at: 6729 6762 7778 9162 9430 1632
Size: 33 1016 1384 268 1917
Fragments arranged by size:
1917 1621 1384 1327 1016 893 596 327
268 171 139 33 23
AflII C'TTAA_G
Cuts at: 820 1017 6967 820
Size: 197 5950 3568
Fragments arranged by size:
5950 3568 197
AflIII A'CryG_T
Cuts at: 1203 2602 2838 2950 4980 8614 9591 1203
Size: 1399 236 112 2030 3634 977 1327
Fragments arranged by size:
3634 2030 1399 1327 977 236 112
AgeI A'CCGG_T
Cuts at: 8546 8546
Size: 9715
AhdI GACnn_n'nnGTC
Cuts at: 6308 8444 6308
Size: 2136 7579
AleI CACnn'nnGTG
Cuts at: 1166 6228 1166
Size: 5062 4653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9715
AlwNI CAG_nnn'CTG
Cuts at: 7458 9030 7458
Size: 1572 8143
ApaLI G'TGCA_C
Cuts at: 7772 8928 7772
Size: 1156 8559
ApoI r'AATT_y
Cuts at: 1493 2369 2428 3626 3649 3683 3761 3956 4630
Size: 876 59 1198 23 34 78 195 674
Cuts at: 4630 5296 5623 5702 6046 6113 6210 7024 7250
Size: 666 327 79 344 67 97 814 226
Cuts at: 7250 7402 9511 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1198 876 814 674 666 344
327 226 195 152 97 79 78 67
59 34 23
AseI AT'TA_AT
Cuts at: 160 1032 4059 8269 160
Size: 872 3027 4210 1606
Fragments arranged by size:
4210 3027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9715
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 3465 4893 5553 7040 1870
Size: 73 421 1101 1428 660 1487 4545
Fragments arranged by size:
4545 1487 1428 1101 660 421 73
BamHI G'GATC_C
Cuts at: 7045 7412 7045
Size: 367 9348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4964 7036 8491 618
Size: 325 217 627 3177 2072 1455 1842
Fragments arranged by size:
3177 2072 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 2280 7034 1391
Size: 766 123 4754 4072
Fragments arranged by size:
4754 4072 766 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2947 4797 5856 6390 928
Size: 971 1048 1850 1059 534 4253
Fragments arranged by size:
4253 1850 1059 1048 971 534
BbvCI CC'TCA_GC
Cuts at: 2210 4427 2210
Size: 2217 7498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5309 5343 7906 7940 1903
Size: 34 3372 34 2563 34 3678
Fragments arranged by size:
3678 3372 2563 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4273 7607 8817 3395
Size: 878 3334 1210 4293
Fragments arranged by size:
4293 3334 1210 878
BclI T'GATC_A
Cuts at: 1548 3202 5527 1548
Size: 1654 2325 5736
Fragments arranged by size:
5736 2325 1654
BfrBI ATG'CAT
Cuts at: 2549 4364 2549
Size: 1815 7900
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2284 8326 136
Size: 107 122 71 631 605 612 6042 1525
Fragments arranged by size:
6042 1525 631 612 605 122 107 71
BglII A'GATC_T
Cuts at: 3026 9710 3026
Size: 6684 3031
BlpI GC'TnA_GC
Cuts at: 2151 7132 2151
Size: 4981 4734
Bme1580I G_kGCm'C
Cuts at: 1790 4598 4967 7776 8932 1790
Size: 2808 369 2809 1156 2573
Fragments arranged by size:
2809 2808 2573 1156 369
BmgBI CAC'GTC
Cuts at: 1318 2444 4981 1318
Size: 1126 2537 6052
Fragments arranged by size:
6052 2537 1126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4668 5580 7446
Size: 416 53 83 399 24 3251 912 1866
Cuts at: 7446 8404 442
Size: 958 1753
Fragments arranged by size:
3251 1866 1753 958 912 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4471 4471
Size: 9715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2340 3210 6000 6063 6237 8375 1239
Size: 1101 870 2790 63 174 2138 2579
Fragments arranged by size:
2790 2579 2138 1101 870 174 63
Bpu10I CC'TnA_GC
Cuts at: 2210 2933 4427 2210
Size: 723 1494 7498
Fragments arranged by size:
7498 1494 723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3961 4166 4282 5362 5426 6083 6494 6882
Size: 1835 205 116 1080 64 657 411 388
Cuts at: 6882 7840 8705 9003 2126
Size: 958 865 298 2838
Fragments arranged by size:
2838 1835 1080 958 865 657 411 388
298 205 116 64
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3387 6593 8378 882
Size: 617 1888 3206 1785 2219
Fragments arranged by size:
3206 2219 1888 1785 617
BsaAI yAC'GTr
Cuts at: 493 1204 2839 6614 7506 493
Size: 711 1635 3775 892 2702
Fragments arranged by size:
3775 2702 1635 892 711
BsaBI GATnn'nnATC
Cuts at: 9709 9709
Size: 9715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5682 7522 7904 8539
Size: 53 83 186 936 4149 1840 382 635
Cuts at: 8539 275
Size: 1451
Fragments arranged by size:
4149 1840 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2098 8148 8546 8820 8967 9378 2098
Size: 6050 398 274 147 411 2435
Fragments arranged by size:
6050 2435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 4991 5021 1178
Size: 30 165 30 690 30 2868 30 5872
Fragments arranged by size:
5872 2868 690 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2224 2311 5015 1186
Size: 1006 32 87 2704 5886
Fragments arranged by size:
5886 2704 1006 87 32
BseYI C'CCAG_C
Cuts at: 2156 2168 4798 8918 9414 2156
Size: 12 2630 4120 496 2457
Fragments arranged by size:
4120 2630 2457 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5246 1163
Size: 573 3510 5632
Fragments arranged by size:
5632 3510 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 7165 7926 8954 1540
Size: 275 186 252 4912 761 1028 2301
Fragments arranged by size:
4912 2301 1028 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 4406 5736 7776 7861 8932 4406
Size: 1330 2040 85 1071 5189
Fragments arranged by size:
5189 2040 1330 1071 85
BsmI GAATG_Cn'
Cuts at: 2854 4780 5455 6154 7226 7319 2854
Size: 1926 675 699 1072 93 5250
Fragments arranged by size:
5250 1926 1072 699 675 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2015 2067 6663 2015
Size: 52 4596 5067
Fragments arranged by size:
5067 4596 52
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2280 4406 4598 4967 5736 7034
Size: 399 367 123 2126 192 369 769 1298
Cuts at: 7034 7776 7861 8932 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2126 1298 1071 769 742 399 369
367 192 123 85
BspEI T'CCGG_A
Cuts at: 2098 9378 2098
Size: 7280 2435
BspHI T'CATG_A
Cuts at: 5673 5713 7604 9334 5673
Size: 40 1891 1730 6054
Fragments arranged by size:
6054 1891 1730 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5849 7070 844
Size: 5005 1221 3489
Fragments arranged by size:
5005 3489 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 7602 1394
Size: 429 5779 3507
Fragments arranged by size:
5779 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2712 4296 5853 6101 8210 8384 65
Size: 1183 1464 1584 1557 248 2109 174 1396
Fragments arranged by size:
2109 1584 1557 1464 1396 1183 248 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8359 8546 1700
Size: 110 6549 187 2869
Fragments arranged by size:
6549 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9715
BssHII G'CGCG_C
Cuts at: 1925 4349 1925
Size: 2424 7291
BssSI C'ACGA_G
Cuts at: 2216 3141 7775 8787 2216
Size: 925 4634 1012 3144
Fragments arranged by size:
4634 3144 1012 925
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5011 794
Size: 4217 5498
BstBI TT'CG_AA
Cuts at: 8519 8519
Size: 9715
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9715
BstXI CCAn_nnnn'nTGG
Cuts at: 2123 4591 2123
Size: 2468 7247
BstYI r'GATC_y
Cuts at: 1972 2307 3026 4150 4624 7045 7412 7797 7814
Size: 335 719 1124 474 2421 367 385 17
Cuts at: 7814 9266 9352 9364 9710 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2421 1977 1452 1124 719 474 385 367
346 335 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2226 4393 2226
Size: 2167 7548
BtgI C'CryG_G
Cuts at: 513 1065 2004 2643 2766 513
Size: 552 939 639 123 7462
Fragments arranged by size:
7462 939 639 552 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 487
Size: 683 56 905 8071
Fragments arranged by size:
8071 905 683 56
BtsI GCAGTG_nn'
Cuts at: 5012 5046 7231 8025 8045 5012
Size: 34 2185 794 20 6682
Fragments arranged by size:
6682 2185 794 34 20
ClaI AT'CG_AT
Cuts at: 1554 2566 1554
Size: 1012 8703
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9680
DraI TTT'AAA
Cuts at: 2728 3096 5895 6403 7019 2728
Size: 368 2799 508 616 5424
Fragments arranged by size:
5424 2799 616 508 368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2955 3460 8722 809
Size: 507 1639 505 5262 1802
Fragments arranged by size:
5262 1802 1639 507 505
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 7162 8051
Size: 54 1425 50 275 18 420 4912 889
Cuts at: 8051 9680 8
Size: 1629 43
Fragments arranged by size:
4912 1629 1425 889 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 7162 1537
Size: 275 438 4912 4090
Fragments arranged by size:
4912 4090 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2312 2491 4130 4212 4416 4725 5097
Size: 915 44 179 1639 82 204 309 372
Cuts at: 5097 5718 6510 6705 7645 1353
Size: 621 792 195 940 3423
Fragments arranged by size:
3423 1639 940 915 792 621 372 309
204 195 179 82 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 8302 8676 8822 387
Size: 1064 138 496 6217 374 146 1280
Fragments arranged by size:
6217 1280 1064 496 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2525 3210 3852 3875 5496 5667
Size: 393 708 185 685 642 23 1621 171
Cuts at: 5667 6000 6063 6237 6263 6402 6729 6762 7778
Size: 333 63 174 26 139 327 33 1016
Cuts at: 7778 8375 9162 9430 1239
Size: 597 787 268 1524
Fragments arranged by size:
1621 1524 1016 787 708 685 642 597
393 333 327 268 185 174 171 139
63 33 26 23
EcoO109I rG'GnC_Cy
Cuts at: 2267 2267
Size: 9715
EcoRI G'AATT_C
Cuts at: 3626 7024 7402 3626
Size: 3398 378 5939
Fragments arranged by size:
5939 3398 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3107 3125 6461 7005 3107
Size: 18 3336 544 5817
Fragments arranged by size:
5817 3336 544 18
FspI TGC'GCA
Cuts at: 8221 8221
Size: 9715
HaeII r_GCGC'y
Cuts at: 1678 2096 2169 3645 8862 1678
Size: 418 73 1476 5217 2531
Fragments arranged by size:
5217 2531 1476 418 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2548 2580 3187
Size: 32 135 32 521 32 423 32 607
Cuts at: 3187 3219 3421 3453 3601 3633 4541 4573 5426
Size: 32 202 32 148 32 908 32 853
Cuts at: 5426 5458 5512 5544 5973 6005 6294 6326 6467
Size: 32 54 32 429 32 289 32 141
Cuts at: 6467 6499 6509 6533 6541 6565 6638 6670 8356
Size: 32 10 24 8 24 73 32 1686
Cuts at: 8356 8388 8430 8462 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1686 908 853 607 521 429 423
289 202 148 141 135 73 54 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
24 24 10 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4384 4986 6171 7059 7305 669
Size: 988 281 2446 602 1185 888 246 3079
Fragments arranged by size:
3079 2446 1185 988 888 602 281 246
HindIII A'AGCT_T
Cuts at: 748 4142 6937 748
Size: 3394 2795 3526
Fragments arranged by size:
3526 3394 2795
HpaI GTT'AAC
Cuts at: 7305 7305
Size: 9715
KpnI G_GTAC'C
Cuts at: 7040 7040
Size: 9715
MfeI C'AATT_G
Cuts at: 6226 7314 6226
Size: 1088 8627
MluI A'CGCG_T
Cuts at: 9591 9591
Size: 9715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8829 9013 1305
Size: 7524 184 2007
Fragments arranged by size:
7524 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7893
Fragments arranged by size:
7893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4212 4359 4443 6228 6335 6837 7673
Size: 648 3046 147 84 1785 107 502 836
Cuts at: 7673 8032 8191 518
Size: 359 159 2042
Fragments arranged by size:
3046 2042 1785 836 648 502 359 159
147 107 84
MspA1I CmG'CkG
Cuts at: 1606 2160 4798 7131 7808 8956 9201 1606
Size: 554 2638 2333 677 1148 245 2120
Fragments arranged by size:
2638 2333 2120 1148 677 554 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2643 2766 513
Size: 552 939 639 123 7462
Fragments arranged by size:
7462 939 639 552 123
NdeI CA'TA_TG
Cuts at: 387 2585 2962 3442 3736 5131 6874 387
Size: 2198 377 480 294 1395 1743 3228
Fragments arranged by size:
3228 2198 1743 1395 480 377 294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9715
NotI GC'GGCC_GC
Cuts at: 2250 7162 2250
Size: 4912 4803
NsiI A_TGCA'T
Cuts at: 2551 4366 2551
Size: 1815 7900
NspI r_CATG'y
Cuts at: 1132 1229 2606 3479 3568 3815 5363 5504 7073
Size: 97 1377 873 89 247 1548 141 1569
Cuts at: 7073 8618 1132
Size: 1545 2229
Fragments arranged by size:
2229 1569 1548 1545 1377 873 247 141
97 89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9715
PciI A'CATG_T
Cuts at: 2602 8614 2602
Size: 6012 3703
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6455 1108
Size: 5347 4368
PmeI GTTT'AAAC
Cuts at: 7019 7019
Size: 9715
PmlI CAC'GTG
Cuts at: 2839 6614 2839
Size: 3775 5940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7784 7816 9297 9329 7784
Size: 32 1481 32 8170
Fragments arranged by size:
8170 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4708 1613
Size: 3095 6620
PsiI TTA'TAA
Cuts at: 3240 5630 7285 3240
Size: 2390 1655 5670
Fragments arranged by size:
5670 2390 1655
PstI C_TGCA'G
Cuts at: 830 5041 7067 830
Size: 4211 2026 3478
Fragments arranged by size:
4211 3478 2026
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9715
PvuII CAG'CTG
Cuts at: 4798 4798
Size: 9715
SalI G'TCGA_C
Cuts at: 1936 7057 1936
Size: 5121 4594
SapI GCTCTTCn'nnn_
Cuts at: 2268 2312 4416 2268
Size: 44 2104 7567
Fragments arranged by size:
7567 2104 44
SbfI CC_TGCA'GG
Cuts at: 7067 7067
Size: 9715
ScaI AGT'ACT
Cuts at: 6076 7963 9675 6076
Size: 1887 1712 6116
Fragments arranged by size:
6116 1887 1712
SexAI A'CCwGG_T
Cuts at: 2571 4825 5539 2571
Size: 2254 714 6747
Fragments arranged by size:
6747 2254 714
SfcI C'TryA_G
Cuts at: 826 1045 2437 5037 7063 8198 8879 9070 826
Size: 219 1392 2600 2026 1135 681 191 1471
Fragments arranged by size:
2600 2026 1471 1392 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9715
SmaI CCC'GGG
Cuts at: 1872 4895 5555 7042 1872
Size: 3023 660 1487 4545
Fragments arranged by size:
4545 3023 1487 660
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2364 3465 3976 4145 4261
Size: 197 926 162 259 1101 511 169 116
Cuts at: 4261 5377 5405 6062 6473 6897 6967 7819 8720
Size: 1116 28 657 411 424 70 852 901
Cuts at: 8720 8982 820
Size: 262 1553
Fragments arranged by size:
1553 1116 1101 926 901 852 657 511
424 411 262 259 197 169 162 116
70 28
SnaBI TAC'GTA
Cuts at: 493 7506 493
Size: 7013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9715
SphI G_CATG'C
Cuts at: 1132 3568 3815 7073 1132
Size: 2436 247 3258 3774
Fragments arranged by size:
3774 3258 2436 247
SspI AAT'ATT
Cuts at: 5 52 2389 7639 5
Size: 47 2337 5250 2081
Fragments arranged by size:
5250 2337 2081 47
StuI AGG'CCT
Cuts at: 1517 2030 2287 4877 1517
Size: 513 257 2590 6355
Fragments arranged by size:
6355 2590 513 257
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 2766 5286 7154 513
Size: 552 939 639 123 2520 1868 3074
Fragments arranged by size:
3074 2520 1868 939 639 552 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 2699 2759 3411 4079 7742 7759
Size: 1255 1056 63 60 652 668 3663 17
Cuts at: 7759 7912 8097 8542 325
Size: 153 185 445 1498
Fragments arranged by size:
3663 1498 1255 1056 668 652 445 185
153 63 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5076 5138 6074
Size: 274 80 33 51 1443 3099 62 936
Cuts at: 6074 7961 9673 96
Size: 1887 1712 138
Fragments arranged by size:
3099 1887 1712 1443 936 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 4069 4101 4795 4827 4991 5023 9675
Size: 32 1448 32 694 32 164 32 4652
Cuts at: 9675 9707 2589
Size: 32 2597
Fragments arranged by size:
4652 2597 1448 694 164 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4379 1316
Size: 3063 6652
XbaI T'CTAG_A
Cuts at: 7051 7051
Size: 9715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5512 6087 5512
Size: 575 9140
XhoI C'TCGA_G
Cuts at: 1943 2364 3465 1943
Size: 421 1101 8193
Fragments arranged by size:
8193 1101 421
XmaI C'CCGG_G
Cuts at: 1870 4893 5553 7040 1870
Size: 3023 660 1487 4545
Fragments arranged by size:
4545 3023 1487 660
XmnI GAAnn'nnTTC
Cuts at: 6006 6444 6914 7844 6006
Size: 438 470 930 7877
Fragments arranged by size:
7877 930 470 438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5683 7523 276
Size: 53 83 186 936 4149 1840 2468
Fragments arranged by size:
4149 2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI