Restriction map for
pFN21AE9000
(Circular) (Six-base) MAPSORT of: pFN21AE9000.seq Check: 7067 from: 1 to: 6268
pFN21AE9000 6268 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3611 1937
Size: 1674 4594
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4331 5715 5983 1632
Size: 2699 1384 268 1917
Fragments arranged by size:
2699 1917 1384 268
AfeI AGC'GCT
Cuts at: 2633 2633
Size: 6268
AflII C'TTAA_G
Cuts at: 820 1017 2743 820
Size: 197 1726 4345
Fragments arranged by size:
4345 1726 197
AflIII A'CryG_T
Cuts at: 1203 2761 2957 3067 5167 6144 1203
Size: 1558 196 110 2100 977 1327
Fragments arranged by size:
2100 1558 1327 977 196 110
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 2336 1166
Size: 1170 5098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 4011 5583 4011
Size: 1572 4696
ApaLI G'TGCA_C
Cuts at: 2166 2230 2800 4325 5481 2166
Size: 64 570 1525 1156 2953
Fragments arranged by size:
2953 1525 1156 570 64
ApoI r'AATT_y
Cuts at: 1493 3577 3803 3955 6064 1493
Size: 2084 226 152 2109 1697
Fragments arranged by size:
2109 2084 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 3593 1870
Size: 73 491 1159 4545
Fragments arranged by size:
4545 1159 491 73
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2438 3589 5044 618
Size: 325 217 627 487 164 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 487 325 217 164
BanII G_rGCy'C
Cuts at: 1391 2102 2115 3587 1391
Size: 711 13 1472 4072
Fragments arranged by size:
4072 1472 711 13
BbeI G_GCGC'C
Cuts at: 2278 2278
Size: 6268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 2119 1548
Size: 571 5697
BfrBI ATG'CAT
Cuts at: 2985 2985
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2856 4879
Size: 107 122 71 631 605 337 847 2023
Cuts at: 4879 136
Size: 1525
Fragments arranged by size:
2023 1525 847 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3685 3685
Size: 6268
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2234 2441 2804 4329 5485 1790
Size: 380 64 207 363 1525 1156 2573
Fragments arranged by size:
2573 1525 1156 380 363 207 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 2479 2496
Size: 416 53 83 399 24 1025 37 17
Cuts at: 2496 3242 3386 3416 3999 4957 442
Size: 746 144 30 583 958 1753
Fragments arranged by size:
1753 1025 958 746 583 416 399 144
83 53 37 30 24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4928 1239
Size: 3689 2579
Bpu10I CC'TnA_GC
Cuts at: 2096 2096
Size: 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2713 4393 5258 5556 2713
Size: 1680 865 298 3425
Fragments arranged by size:
3425 1680 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2395 2676 2990 3505 4931 882
Size: 617 896 281 314 515 1426 2219
Fragments arranged by size:
2219 1426 896 617 515 314 281
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 6262 6262
Size: 6268
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2275 4075 4457 5092
Size: 53 83 186 936 742 1800 382 635
Cuts at: 5092 275
Size: 1451
Fragments arranged by size:
1800 1451 936 742 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2649 4701 5099 5373 5520 5931 2649
Size: 2052 398 274 147 411 2986
Fragments arranged by size:
2986 2052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 1178
Size: 30 165 30 1167 30 4846
Fragments arranged by size:
4846 1167 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6268
BseYI C'CCAG_C
Cuts at: 2363 5471 5967 2363
Size: 3108 496 2664
Fragments arranged by size:
3108 2664 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2916 3321 1163
Size: 573 1180 405 4110
Fragments arranged by size:
4110 1180 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2170 2234 2804 4329 4414 5485 2102
Size: 68 64 570 1525 85 1071 2885
Fragments arranged by size:
2885 1525 1071 570 85 68 64
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2115 2170 2234 2441 2804 3587
Size: 399 312 13 55 64 207 363 783
Cuts at: 3587 4329 4414 5485 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 363 312 207
85 64 55 13
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 2674 4157 5887 2674
Size: 1483 1730 3055
Fragments arranged by size:
3055 1730 1483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3623 844
Size: 2779 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2129 2397 3273 3513 4763 4937 65
Size: 1183 881 268 876 240 1250 174 1396
Fragments arranged by size:
1396 1250 1183 881 876 268 240 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2238 794
Size: 1444 4824
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2338
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 3598 3965 4350 4367 5819 5905 5917 6263
Size: 1626 367 385 17 1452 86 12 346
Cuts at: 6263 1972
Size: 1977
Fragments arranged by size:
1977 1626 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2160 2270 2160
Size: 110 6158
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 2334 3168 513
Size: 552 939 102 228 834 3613
Fragments arranged by size:
3613 939 834 552 228 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2344 487
Size: 683 56 1118 4411
Fragments arranged by size:
4411 1118 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 2713 2802 3414 3784 4578 4598 2461
Size: 252 89 612 370 794 20 4131
Fragments arranged by size:
4131 794 612 370 252 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DraIII CAC_nnn'GTG
Cuts at: 2845 2845
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2315 3080 3715
Size: 54 1425 50 275 18 485 765 635
Cuts at: 3715 4604 6233 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 765 635 485 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2988 4198 1353
Size: 1635 1210 3423
Fragments arranged by size:
3423 1635 1210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4855 5229 5375 387
Size: 1064 138 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2100 2100
Size: 6268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4331 4928 5715 5983 1239
Size: 393 2699 597 787 268 1524
Fragments arranged by size:
2699 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2033
Size: 6268
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2278 2635 5415 1678
Size: 600 357 2780 2531
Fragments arranged by size:
2780 2531 600 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 4909 4941 4983
Size: 32 135 32 996 32 2309 32 42
Cuts at: 4983 5015 1373
Size: 32 2626
Fragments arranged by size:
2626 2309 996 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2415 3307 3397 3612 3858 669
Size: 988 281 477 892 90 215 246 3079
Fragments arranged by size:
3079 988 892 477 281 246 215 90
HindIII A'AGCT_T
Cuts at: 748 2046 2247 3543 748
Size: 1298 201 1296 3473
Fragments arranged by size:
3473 1298 1296 201
HpaI GTT'AAC
Cuts at: 3307 3858 3307
Size: 551 5717
KasI G'GCGC_C
Cuts at: 2274 2274
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5382 5566 1305
Size: 4077 184 2007
Fragments arranged by size:
4077 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3082 10
Size: 54 1425 343 1250 3196
Fragments arranged by size:
3196 1425 1250 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2227 2336 2471 2529 2595 3476 4226
Size: 648 1061 109 135 58 66 881 750
Cuts at: 4226 4585 4744 518
Size: 359 159 2042
Fragments arranged by size:
2042 1061 881 750 648 359 159 135
109 66 58
MspA1I CmG'CkG
Cuts at: 1606 3263 3684 4361 5509 5754 1606
Size: 1657 421 677 1148 245 2120
Fragments arranged by size:
2120 1657 1148 677 421 245
NarI GG'CG_CC
Cuts at: 2275 2275
Size: 6268
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NsiI A_TGCA'T
Cuts at: 2987 2987
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2765 2961 2985 3071 3529 3626 5171
Size: 97 1536 196 24 86 458 97 1545
Cuts at: 5171 1132
Size: 2229
Fragments arranged by size:
2229 1545 1536 458 196 97 97 86
24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 2761 2957 3067 5167 2761
Size: 196 110 2100 3862
Fragments arranged by size:
3862 2100 196 110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PstI C_TGCA'G
Cuts at: 830 2243 3620 830
Size: 1413 1377 3478
Fragments arranged by size:
3478 1413 1377
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
PvuII CAG'CTG
Cuts at: 3263 3263
Size: 6268
SacI G_AGCT'C
Cuts at: 2102 2102
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2239 3516 3616 4751 5432 5623 826
Size: 219 1194 1277 100 1135 681 191 1471
Fragments arranged by size:
1471 1277 1194 1135 681 219 191 100
SfoI GGC'GCC
Cuts at: 2276 2276
Size: 6268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2728 2743 4372 5273 5535 820
Size: 197 926 785 15 1629 901 262 1553
Fragments arranged by size:
1629 1553 926 901 785 262 197 15
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3529 3626 1132
Size: 2397 97 3774
Fragments arranged by size:
3774 2397 97
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 2549 2734 1517
Size: 1032 185 5051
Fragments arranged by size:
5051 1032 185
StyI C'CwwG_G
Cuts at: 513 1065 2004 3563 3707 513
Size: 552 939 1559 144 3074
Fragments arranged by size:
3074 1559 939 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4514 6226 96
Size: 274 80 33 51 1443 2537 1712 138
Fragments arranged by size:
2537 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2570 2602 6228 6260 2326
Size: 32 212 32 3626 32 2334
Fragments arranged by size:
3626 2334 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2485 3238 1316
Size: 1169 753 4346
Fragments arranged by size:
4346 1169 753
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 2549 2723 4397 2549
Size: 174 1674 4420
Fragments arranged by size:
4420 1674 174
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I EcoO109I EcoRV FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI