Restriction map for
pFN21AE8000
(Circular) (Six-base) MAPSORT of: pFN21AE8000.seq Check: 7484 from: 1 to: 6007
pFN21AE8000 6007 bp
With 182 enzymes: SgfI *
February 29, 2012 12:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2930 2930
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2779 2852 3014 3143 3197 4070 5454 5722
Size: 1147 73 162 129 54 873 1384 268
Cuts at: 5722 1632
Size: 1917
Fragments arranged by size:
1917 1384 1147 873 268 162 129 73
54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 2174 2739 2751 4906 5883 1203
Size: 971 565 12 2155 977 1327
Fragments arranged by size:
2155 1327 977 971 565 12
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 2065 4736 2065
Size: 2671 3336
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 2069 2416 2431 2547 2670 3235 3750 5322 2069
Size: 347 15 116 123 565 515 1572 2754
Fragments arranged by size:
2754 1572 565 515 347 123 116 15
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2515 2978 3316 3542 3694 5803 1493
Size: 1022 463 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1022 463 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 2249 3328 4783 618
Size: 325 217 627 445 17 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 627 445 325 217 17
BanII G_rGCy'C
Cuts at: 1391 2970 3204 3326 1391
Size: 1579 234 122 4072
Fragments arranged by size:
4072 1579 234 122
BbeI G_GCGC'C
Cuts at: 2253 2253
Size: 6007
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 2937 928
Size: 971 377 661 3998
Fragments arranged by size:
3998 971 661 377
BbvCI CC'TCA_GC
Cuts at: 2228 2228
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 2337 2376 2454 1548
Size: 789 39 78 5101
Fragments arranged by size:
5101 789 78 39
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2617 2709 2820 6002 2617
Size: 92 111 3182 2622
Fragments arranged by size:
3182 2622 111 92
BlpI GC'TnA_GC
Cuts at: 2537 3219 3424 2537
Size: 682 205 5120
Fragments arranged by size:
5120 682 205
Bme1580I G_kGCm'C
Cuts at: 1790 2237 4068 5224 1790
Size: 447 1831 1156 2573
Fragments arranged by size:
2573 1831 1156 447
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2079 2426 3738
Size: 416 53 83 399 24 662 347 1312
Cuts at: 3738 4696 442
Size: 958 1753
Fragments arranged by size:
1753 1312 958 662 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2984 4667 1239
Size: 1094 651 1683 2579
Fragments arranged by size:
2579 1683 1094 651
Bpu10I CC'TnA_GC
Cuts at: 2228 2228
Size: 6007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2008 2641 4670 882
Size: 617 509 633 2029 2219
Fragments arranged by size:
2219 2029 633 617 509
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 2453 6001 2453
Size: 3548 2459
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 3814 4196 4831
Size: 53 83 186 936 717 1564 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1564 1451 936 717 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2241 2610 4440 4838 5112 5259 5670 2241
Size: 369 1830 398 274 147 411 2578
Fragments arranged by size:
2578 1830 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2807 2837 1178
Size: 30 165 30 1404 30 4348
Fragments arranged by size:
4348 1404 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2453 3029 3032 1186
Size: 1189 78 576 3 4161
Fragments arranged by size:
4161 1189 576 78 3
BseYI C'CCAG_C
Cuts at: 2686 3150 3228 5210 5706 2686
Size: 464 78 1982 496 2987
Fragments arranged by size:
2987 1982 496 464 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 1163
Size: 573 708 4726
Fragments arranged by size:
4726 708 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2627 3204 4068 4153 5224 2627
Size: 577 864 85 1071 3410
Fragments arranged by size:
3410 1071 864 577 85
BsmI GAATG_Cn'
Cuts at: 3143 3518 3611 3143
Size: 375 93 5539
Fragments arranged by size:
5539 375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2627 2970 3204 3326 4068 4153
Size: 399 447 390 343 234 122 742 85
Cuts at: 4153 5224 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 390 343 234
122 85
BspEI T'CCGG_A
Cuts at: 2241 2610 5670 2241
Size: 369 3060 2578
Fragments arranged by size:
3060 2578 369
BspHI T'CATG_A
Cuts at: 2169 3896 5626 2169
Size: 1727 1730 2550
Fragments arranged by size:
2550 1730 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 3362 844
Size: 2086 432 3489
Fragments arranged by size:
3489 2086 432
BsrBI CCG'CTC
Cuts at: 1394 1823 2319 3894 1394
Size: 429 496 1575 3507
Fragments arranged by size:
3507 1575 496 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2910 4651 4838 1700
Size: 110 1100 1741 187 2869
Fragments arranged by size:
2869 1741 1100 187 110
BsrGI T'GTAC_A
Cuts at: 96 2047 96
Size: 1951 4056
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2547 794
Size: 1753 4254
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2617 2709 2820 3337 3704 4089 4106 5558
Size: 645 92 111 517 367 385 17 1452
Cuts at: 5558 5644 5656 6002 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 645 517 385 367 346 111
92 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 4165
Fragments arranged by size:
4165 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 2671 2934 3523 4317 4337 2548
Size: 123 263 589 794 20 4218
Fragments arranged by size:
4218 794 589 263 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 3085 3085
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3454 4343
Size: 54 1425 50 275 18 1078 546 889
Cuts at: 4343 5972 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1078 889 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3053 3937 1353
Size: 1700 884 3423
Fragments arranged by size:
3423 1700 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3202 3202
Size: 6007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2779 2852 2984 3014 3143 3197
Size: 393 701 446 73 132 30 129 54
Cuts at: 3197 4070 4667 5454 5722 1239
Size: 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 701 597 446 393 268
132 129 73 54 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2794 2831 3278 2794
Size: 37 447 5523
Fragments arranged by size:
5523 447 37
EcoO109I rG'GnC_Cy
Cuts at: 2114 2572 2114
Size: 458 5549
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 2303 3242 4513 2303
Size: 939 1271 3797
Fragments arranged by size:
3797 1271 939
HaeII r_GCGC'y
Cuts at: 1678 2253 5154 1678
Size: 575 2901 2531
Fragments arranged by size:
2901 2531 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2392 2424 2458
Size: 32 135 32 479 32 309 32 34
Cuts at: 2458 2490 2609 2641 2807 2839 4648 4680 4722
Size: 32 119 32 166 32 1809 32 42
Cuts at: 4722 4754 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 479 309 166 135 119 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2745 2858 3351 3597 669
Size: 988 281 154 653 113 493 246 3079
Fragments arranged by size:
3079 988 653 493 281 246 154 113
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KasI G'GCGC_C
Cuts at: 2249 2249
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2089 2370 5121 5305 1305
Size: 784 281 2751 184 2007
Fragments arranged by size:
2751 2007 784 281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4185
Fragments arranged by size:
4185 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2163 3188 3965 4324 4483 518
Size: 648 843 154 1025 777 359 159 2042
Fragments arranged by size:
2042 1025 843 777 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 2446 2647 3007 3097 3232 3423 4100 5248
Size: 840 201 360 90 135 191 677 1148
Cuts at: 5248 5493 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 840 677 360 245 201 191
135 90
NaeI GCC'GGC
Cuts at: 2912 2912
Size: 6007
NarI GG'CG_CC
Cuts at: 2250 2250
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4516
Fragments arranged by size:
4516 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NgoMIV G'CCGG_C
Cuts at: 2910 2910
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2178 2743 2755 2992 3365 4910 1132
Size: 97 949 565 12 237 373 1545 2229
Fragments arranged by size:
2229 1545 949 565 373 237 97 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 2174 2739 2751 4906 2174
Size: 565 12 2155 3275
Fragments arranged by size:
3275 2155 565 12
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PfoI T'CCnGG_A
Cuts at: 2074 3246 2074
Size: 1172 4835
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2114 2114
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2571 2646 2941 3006 3078 3102 3359 830
Size: 1741 75 295 65 72 24 257 3478
Fragments arranged by size:
3478 1741 295 257 75 72 65 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2647 3007 3097 2647
Size: 360 90 5557
Fragments arranged by size:
5557 360 90
SacI G_AGCT'C
Cuts at: 3204 3204
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2567 2642 2937 3002 3074 3098 3355
Size: 219 1522 75 295 65 72 24 257
Cuts at: 3355 4490 5171 5362 826
Size: 1135 681 191 1471
Fragments arranged by size:
1522 1471 1135 681 295 257 219 191
75 72 65 24
SfoI GGC'GCC
Cuts at: 2251 2251
Size: 6007
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 1517
Size: 774 5233
StyI C'CwwG_G
Cuts at: 513 1065 2004 3446 513
Size: 552 939 1442 3074
Fragments arranged by size:
3074 1442 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3242 4034 4051 4204 4389 4834 325
Size: 1255 1662 792 17 153 185 445 1498
Fragments arranged by size:
1662 1498 1255 792 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2047 4253 5965
Size: 274 80 33 51 1443 70 2206 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
2206 1712 1443 274 138 80 70 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 5967 5999 2377
Size: 32 3558 32 2385
Fragments arranged by size:
3558 2385 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI