Restriction map for
pFN21AE6893
(Circular) (Six-base) MAPSORT of: pFN21AE6893.seq Check: 6595 from: 1 to: 5800
pFN21AE6893 5800 bp
With 182 enzymes: SgfI *
April 28, 2014 11:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2253 2973 3143 1937
Size: 316 720 170 4594
Fragments arranged by size:
4594 720 316 170
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 3032 3863 5247 5515 1632
Size: 878 522 831 1384 268 1917
Fragments arranged by size:
1917 1384 878 831 522 268
AfeI AGC'GCT
Cuts at: 2696 2696
Size: 5800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 2452 4529 2452
Size: 2077 3723
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4582
AlwNI CAG_nnn'CTG
Cuts at: 3002 3543 5115 3002
Size: 541 1572 3687
Fragments arranged by size:
3687 1572 541
ApaI G_GGCC'C
Cuts at: 2195 2195
Size: 5800
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3060 4354 160
Size: 872 2028 1294 1606
Fragments arranged by size:
2028 1606 1294 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 3125 1870
Size: 73 1101 81 4545
Fragments arranged by size:
4545 1101 81 73
AvrII C'CTAG_G
Cuts at: 2110 2110
Size: 5800
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2927 3121 4576 618
Size: 325 217 627 453 687 194 1455 1842
Fragments arranged by size:
1842 1455 687 627 453 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2195 3119 1391
Size: 804 924 4072
Fragments arranged by size:
4072 924 804
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BbvCI CC'TCA_GC
Cuts at: 2691 2691
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2629 2663 3991 4025 1903
Size: 34 692 34 1328 34 3678
Fragments arranged by size:
3678 1328 692 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BfrBI ATG'CAT
Cuts at: 2353 2353
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 2954 4411
Size: 107 122 71 631 605 691 591 1457
Cuts at: 4411 136
Size: 1525
Fragments arranged by size:
1525 1457 691 631 605 591 122 107
71
BglII A'GATC_T
Cuts at: 3015 5795 3015
Size: 2780 3020
BlpI GC'TnA_GC
Cuts at: 3093 3217 3093
Size: 124 5676
Bme1580I G_kGCm'C
Cuts at: 1790 2195 2758 3861 5017 1790
Size: 405 563 1103 1156 2573
Fragments arranged by size:
2573 1156 1103 563 405
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 3531 4489
Size: 416 53 83 399 24 733 1381 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1381 958 733 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
Bpu10I CC'TnA_GC
Cuts at: 2691 2691
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2494 3925 4790 5088 2494
Size: 1431 865 298 3206
Fragments arranged by size:
3206 1431 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2659 3591 493
Size: 711 1455 932 2702
Fragments arranged by size:
2702 1455 932 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2652 3607 3989 4624
Size: 53 83 186 936 1119 955 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1451 1119 955 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2013 4233 4631 4905 5052 5463 2013
Size: 2220 398 274 147 411 2350
Fragments arranged by size:
2350 2220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2008 2038 2955 2985 1178
Size: 30 165 30 605 30 917 30 3993
Fragments arranged by size:
3993 917 605 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2194 2474 1186
Size: 1008 280 4512
Fragments arranged by size:
4512 1008 280
BseYI C'CCAG_C
Cuts at: 2217 2313 2599 5003 5499 2217
Size: 96 286 2404 496 2518
Fragments arranged by size:
2518 2404 496 286 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2549 1163
Size: 573 813 4414
Fragments arranged by size:
4414 813 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2326 3250 4011 5039 1540
Size: 275 186 325 924 761 1028 2301
Fragments arranged by size:
2301 1028 924 761 325 275 186
BsiHKAI G_wGCw'C
Cuts at: 3861 3946 5017 3861
Size: 85 1071 4644
Fragments arranged by size:
4644 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2758 3119 3861 3946 5017 1391
Size: 399 405 563 361 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 405 399 361 85
BspEI T'CCGG_A
Cuts at: 2013 5463 2013
Size: 3450 2350
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 3155 844
Size: 1827 484 3489
Fragments arranged by size:
3489 1827 484
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 4444 4631 1700
Size: 110 455 2179 187 2869
Fragments arranged by size:
2869 2179 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2596 2686 794
Size: 1802 90 3908
Fragments arranged by size:
3908 1802 90
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 2782 1184
Size: 1598 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2479 2544 3015 3130 3497 3882 3899 5351
Size: 507 65 471 115 367 385 17 1452
Cuts at: 5351 5437 5449 5795 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 507 471 385 367 346 115
86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2423 2665 2739 2873 513
Size: 552 1358 242 74 134 3440
Fragments arranged by size:
3440 1358 552 242 134 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2201 2850 2868 487
Size: 683 56 975 649 18 3419
Fragments arranged by size:
3419 975 683 649 56 18
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DraIII CAC_nnn'GTG
Cuts at: 2424 2901 2424
Size: 477 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2263 2323
Size: 54 1425 50 275 18 330 103 60
Cuts at: 2323 2979 3247 4136 5765 8
Size: 656 268 889 1629 43
Fragments arranged by size:
1629 1425 889 656 330 275 268 103
60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2323 3247 1537
Size: 275 511 924 4090
Fragments arranged by size:
4090 924 511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2212 2577 3730 1353
Size: 859 365 1153 3423
Fragments arranged by size:
3423 1153 859 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 2976 4387 4761 4907 387
Size: 1064 138 1011 376 1411 374 146 1280
Fragments arranged by size:
1411 1280 1064 1011 376 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 3032 3863 4460 5247 5515 1239
Size: 393 878 522 831 597 787 268 1524
Fragments arranged by size:
1524 878 831 787 597 522 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2192 2192
Size: 5800
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2698 2724 4947 1678
Size: 1020 26 2223 2531
Fragments arranged by size:
2531 2223 1020 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2113 2145 2438
Size: 32 135 32 436 32 73 32 293
Cuts at: 2438 2470 2955 2987 4441 4473 4515 4547 1373
Size: 32 485 32 1454 32 42 32 2626
Fragments arranged by size:
2626 1454 485 436 293 135 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2436 4914 5098 1305
Size: 1131 2478 184 2007
Fragments arranged by size:
2478 2007 1131 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2981 10
Size: 54 1425 343 330 819 2829
Fragments arranged by size:
2829 1425 819 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2915 3758 4117 4276 518
Size: 648 1749 843 359 159 2042
Fragments arranged by size:
2042 1749 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3216 3893 5041 5286 1606
Size: 1610 677 1148 245 2120
Fragments arranged by size:
2120 1610 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2739 513
Size: 552 1674 3574
Fragments arranged by size:
3574 1674 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NsiI A_TGCA'T
Cuts at: 2355 2355
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2357 3158 4703 1132
Size: 97 1128 801 1545 2229
Fragments arranged by size:
2229 1545 1128 801 97
PasI CC'CwG_GG
Cuts at: 1348 2830 1348
Size: 1482 4318
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2745 1108
Size: 1637 4163
PfoI T'CCnGG_A
Cuts at: 2535 2535
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PmlI CAC'GTG
Cuts at: 2659 2659
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2191 2191
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2439 2682 3152 830
Size: 1609 243 470 3478
Fragments arranged by size:
3478 1609 470 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2435 2678 3148 4283 4964 5155 826
Size: 219 1390 243 470 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 681 470 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2473 3904 4805 5067 820
Size: 197 926 530 1431 901 262 1553
Fragments arranged by size:
1553 1431 926 901 530 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 2357 3158 1132
Size: 1225 801 3774
Fragments arranged by size:
3774 1225 801
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2115 1517
Size: 598 5202
StyI C'CwwG_G
Cuts at: 513 1065 2110 2739 3239 513
Size: 552 1045 629 500 3074
Fragments arranged by size:
3074 1045 629 552 500
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3040 3827 3844 3997 4182 4627 325
Size: 1255 1460 787 17 153 185 445 1498
Fragments arranged by size:
1498 1460 1255 787 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 5760 5792 2213
Size: 32 3515 32 2221
Fragments arranged by size:
3515 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2969 1316
Size: 1653 4147
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2663 2794 2663
Size: 131 5669
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI