Restriction map for
pFN21AE6821
(Circular) (Six-base) MAPSORT of: pFN21AE6821.seq Check: 778 from: 1 to: 5842
pFN21AE6821 5842 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3009 3009
Size: 5842
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 2139 2232 3163 2139
Size: 93 931 4818
Fragments arranged by size:
4818 931 93
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3905 5289 5557 1632
Size: 2273 1384 268 1917
Fragments arranged by size:
2273 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 2690 4673 2690
Size: 1983 3859
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 2247 2392 2596 3585 5157 2247
Size: 145 204 989 1572 2932
Fragments arranged by size:
2932 1572 989 204 145
ApaI G_GGCC'C
Cuts at: 2059 2296 2059
Size: 237 5605
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 2458 3151 3377 3529 5638 1493
Size: 965 693 226 152 2109 1697
Fragments arranged by size:
2109 1697 965 693 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 3167 1870
Size: 73 1224 4545
Fragments arranged by size:
4545 1224 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2139 2232 2626 2768 2997
Size: 325 217 627 352 93 394 142 229
Cuts at: 2997 3055 3163 4618 618
Size: 58 108 1455 1842
Fragments arranged by size:
1842 1455 627 394 352 325 229 217
142 108 93 58
BanII G_rGCy'C
Cuts at: 1391 2059 2296 2529 3093 3161 1391
Size: 668 237 233 564 68 4072
Fragments arranged by size:
4072 668 564 237 233 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3050 928
Size: 971 1151 3720
Fragments arranged by size:
3720 1151 971
BbvCI CC'TCA_GC
Cuts at: 2205 3049 2205
Size: 844 4998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4033 4067 1903
Size: 34 50 34 2012 34 3678
Fragments arranged by size:
3678 2012 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2415 4453
Size: 107 122 71 631 605 391 352 2038
Cuts at: 4453 136
Size: 1525
Fragments arranged by size:
2038 1525 631 605 391 352 122 107
71
BglII A'GATC_T
Cuts at: 2433 5837 2433
Size: 3404 2438
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2296 2771 3060 3903 5059 1790
Size: 269 237 475 289 843 1156 2573
Fragments arranged by size:
2573 1156 843 475 289 269 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 3573 4531
Size: 416 53 83 399 24 1637 519 958
Cuts at: 4531 442
Size: 1753
Fragments arranged by size:
1753 1637 958 519 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2212 2269 2513 2768 2801 4502 1239
Size: 760 213 57 244 255 33 1701 2579
Fragments arranged by size:
2579 1701 760 255 244 213 57 33
Bpu10I CC'TnA_GC
Cuts at: 2205 3049 3087 2205
Size: 844 38 4960
Fragments arranged by size:
4960 844 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2879 2909 3967 4832 5130 2396
Size: 483 30 1058 865 298 3108
Fragments arranged by size:
3108 1058 865 483 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4505 882
Size: 617 3006 2219
Fragments arranged by size:
3006 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3649 4031 4666 275
Size: 53 83 186 936 2116 382 635 1451
Fragments arranged by size:
2116 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2136 2690 4275 4673 4947 5094 5505 2136
Size: 554 1585 398 274 147 411 2473
Fragments arranged by size:
2473 1585 554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2210 2240 1178
Size: 30 165 30 741 30 36 30 4780
Fragments arranged by size:
4780 741 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2597 3083 1186
Size: 1036 375 486 3945
Fragments arranged by size:
3945 1036 486 375
BseYI C'CCAG_C
Cuts at: 2170 2425 2682 5045 5541 2170
Size: 255 257 2363 496 2471
Fragments arranged by size:
2471 2363 496 257 255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 1163
Size: 573 865 4404
Fragments arranged by size:
4404 865 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2343 3292 4053 5081 1540
Size: 275 186 342 949 761 1028 2301
Fragments arranged by size:
2301 1028 949 761 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2151 2529 3093 3903 3988 5059 2130
Size: 21 378 564 810 85 1071 2913
Fragments arranged by size:
2913 1071 810 564 378 85 21
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2130 2151 2296 2529 2771 3060
Size: 399 269 71 21 145 233 242 289
Cuts at: 3060 3093 3161 3903 3988 5059 1391
Size: 33 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 269 242 233
145 85 71 68 33 21
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2577 2962 3009 3197 844
Size: 1733 385 47 188 3489
Fragments arranged by size:
3489 1733 385 188 47
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2937 4337 4511 65
Size: 1183 1689 1400 174 1396
Fragments arranged by size:
1689 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2690 4486 4673 1700
Size: 110 880 1796 187 2869
Fragments arranged by size:
2869 1796 880 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 2542 4646 2542
Size: 2104 3738
BstEII G'GTnAC_C
Cuts at: 1184 2095 2693 1184
Size: 911 598 4333
Fragments arranged by size:
4333 911 598
BstYI r'GATC_y
Cuts at: 1972 2433 3172 3539 3924 3941 5393 5479 5491
Size: 461 739 367 385 17 1452 86 12
Cuts at: 5491 5837 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 739 461 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2287 2473 513
Size: 552 1222 186 3882
Fragments arranged by size:
3882 1222 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 2589 3358 4152 4172 2448
Size: 141 769 794 20 4118
Fragments arranged by size:
4118 794 769 141 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807
Size: 54 1425 50 275 18 1459 889 1629
Cuts at: 5807 8
Size: 43
Fragments arranged by size:
1629 1459 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2879 3015 3772 1353
Size: 1526 136 757 3423
Fragments arranged by size:
3423 1526 757 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2273 4429 4803 4949 387
Size: 1064 138 684 2156 374 146 1280
Fragments arranged by size:
2156 1280 1064 684 374 146 138
EcoICRI GAG'CTC
Cuts at: 2527 3091 2527
Size: 564 5278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2212 2269 2513 2768 2801 3905
Size: 393 367 213 57 244 255 33 1104
Cuts at: 3905 4502 5289 5557 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1104 787 597 393 367 268 255
244 213 57 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2384 3005 2204
Size: 180 621 5041
Fragments arranged by size:
5041 621 180
EcoO109I rG'GnC_Cy
Cuts at: 2055 2292 2293 2416 2055
Size: 237 1 123 5481
Fragments arranged by size:
5481 237 123 1
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
EcoRV GAT'ATC
Cuts at: 2504 2504
Size: 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 5842
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 4989 1678
Size: 3311 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4483 4515 4557 4589 1373
Size: 32 135 32 2911 32 42 32 2626
Fragments arranged by size:
2911 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 2143 2236 3167 2143
Size: 93 931 4818
Fragments arranged by size:
4818 931 93
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5140 1305
Size: 3651 184 2007
Fragments arranged by size:
3651 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 3800 4159 4318 518
Size: 648 1194 1440 359 159 2042
Fragments arranged by size:
2042 1440 1194 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2429 2593 2761 3053 3258 3935 5083
Size: 564 259 164 168 292 205 677 1148
Cuts at: 5083 5328 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 564 292 259 245 205
168 164
NcoI C'CATG_G
Cuts at: 513 1065 2473 513
Size: 552 1408 3882
Fragments arranged by size:
3882 1408 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 3200 4745 1132
Size: 97 1971 1545 2229
Fragments arranged by size:
2229 1971 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2043 1348
Size: 695 5147
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4471
PfoI T'CCnGG_A
Cuts at: 2667 2778 2667
Size: 111 5731
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspOMI G'GGCC_C
Cuts at: 2055 2292 2055
Size: 237 5605
PstI C_TGCA'G
Cuts at: 830 2169 2198 2443 2959 3194 830
Size: 1339 29 245 516 235 3478
Fragments arranged by size:
3478 1339 516 245 235 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2170 2429 2170
Size: 259 5583
SacI G_AGCT'C
Cuts at: 2529 3093 2529
Size: 564 5278
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SexAI A'CCwGG_T
Cuts at: 2142 2142
Size: 5842
SfcI C'TryA_G
Cuts at: 826 1045 2165 2194 2439 2955 3190 4325 5006
Size: 219 1120 29 245 516 235 1135 681
Cuts at: 5006 5197 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1120 681 516 245 235 219
191 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 2415 2415
Size: 5842
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2858 2888 3946 4847 5109
Size: 197 926 468 447 30 1058 901 262
Cuts at: 5109 820
Size: 1553
Fragments arranged by size:
1553 1058 926 901 468 447 262 197
30
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2070 2473 3281 513
Size: 552 1005 403 808 3074
Fragments arranged by size:
3074 1005 808 552 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 2210 2242 2382 2414 5802 5834 2144
Size: 32 34 32 140 32 3388 32 2152
Fragments arranged by size:
3388 2152 140 34 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 3971 3971
Size: 5842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SfoI
SrfI SwaI XcmI