Restriction map for
pFN21AE6794
(Circular) (Six-base) MAPSORT of: pFN21AE6794.seq Check: 7789 from: 1 to: 8101
pFN21AE6794 8101 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5911 278
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2162 2259 4786 4980 5444 1937
Size: 225 97 2527 194 464 4594
Fragments arranged by size:
4594 2527 464 225 194 97
Acc65I G'GTAC_C
Cuts at: 5422 5422
Size: 8101
AclI AA'CG_TT
Cuts at: 1563 2854 6228 6601 1563
Size: 1291 3374 373 3063
Fragments arranged by size:
3374 3063 1291 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5060 5334 6164 7548 7816 1632
Size: 3428 274 830 1384 268 1917
Fragments arranged by size:
3428 1917 1384 830 274 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7904
AflIII A'CryG_T
Cuts at: 1203 7000 7977 1203
Size: 5797 977 1327
Fragments arranged by size:
5797 1327 977
AgeI A'CCGG_T
Cuts at: 6932 6932
Size: 8101
AhdI GACnn_n'nnGTC
Cuts at: 5351 6830 5351
Size: 1479 6622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4205 4387 4877 1184
Size: 3021 182 490 4408
Fragments arranged by size:
4408 3021 490 182
AlwNI CAG_nnn'CTG
Cuts at: 2036 4717 5844 7416 2036
Size: 2681 1127 1572 2721
Fragments arranged by size:
2721 2681 1572 1127
ApaI G_GGCC'C
Cuts at: 4877 4877
Size: 8101
ApaLI G'TGCA_C
Cuts at: 6158 7314 6158
Size: 1156 6945
ApoI r'AATT_y
Cuts at: 1493 2173 2218 2672 3113 3272 3934 4294 4423
Size: 680 45 454 441 159 662 360 129
Cuts at: 4423 4845 5410 5636 5788 7897 1493
Size: 422 565 226 152 2109 1697
Fragments arranged by size:
2109 1697 680 662 565 454 441 422
360 226 159 152 129 45
AseI AT'TA_AT
Cuts at: 160 1032 2877 3168 4259 6655 160
Size: 872 1845 291 1091 2396 1606
Fragments arranged by size:
2396 1845 1606 1091 872 291
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 5426 1870
Size: 73 3483 4545
Fragments arranged by size:
4545 3483 73
BamHI G'GATC_C
Cuts at: 5431 5798 5431
Size: 367 7734
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4384 4463 5422 6877 618
Size: 325 217 627 2597 79 959 1455 1842
Fragments arranged by size:
2597 1842 1455 959 627 325 217 79
BanII G_rGCy'C
Cuts at: 1391 3125 3181 4104 4877 5420 1391
Size: 1734 56 923 773 543 4072
Fragments arranged by size:
4072 1734 923 773 543 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3674 3966 5190 928
Size: 971 1775 292 1224 3839
Fragments arranged by size:
3839 1775 1224 971 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6292 6326 1903
Size: 34 50 34 4271 34 3678
Fragments arranged by size:
4271 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5047 5215 5993 7203 5047
Size: 168 778 1210 5945
Fragments arranged by size:
5945 1210 778 168
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6712 136
Size: 107 122 71 631 605 5040 1525
Fragments arranged by size:
5040 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8096 8096
Size: 8101
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 4877 6162 7318 1790
Size: 3087 1285 1156 2573
Fragments arranged by size:
3087 2573 1285 1156
BmgBI CAC'GTC
Cuts at: 1318 5070 1318
Size: 3752 4349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 4044 5832
Size: 416 53 83 399 24 853 1774 1788
Cuts at: 5832 6790 442
Size: 958 1753
Fragments arranged by size:
1788 1774 1753 958 853 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2013 2067 3810 6761 1239
Size: 774 54 1743 2951 2579
Fragments arranged by size:
2951 2579 1743 774 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3303 3657 5141 6226 7091 7389 2729
Size: 574 354 1484 1085 865 298 3441
Fragments arranged by size:
3441 1484 1085 865 574 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 3980 6764 882
Size: 617 1506 975 2784 2219
Fragments arranged by size:
2784 2219 1506 975 617
BsaAI yAC'GTr
Cuts at: 493 1204 2585 3404 5892 493
Size: 711 1381 819 2488 2702
Fragments arranged by size:
2702 2488 1381 819 711
BsaBI GATnn'nnATC
Cuts at: 3381 3789 8095 3381
Size: 408 4306 3387
Fragments arranged by size:
4306 3387 408
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5908 6290 6925 275
Size: 53 83 186 936 4375 382 635 1451
Fragments arranged by size:
4375 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6534 6932 7206 7353 7764 6534
Size: 398 274 147 411 6871
Fragments arranged by size:
6871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2781 2811 3168 3198 1178
Size: 30 165 30 1378 30 357 30 6081
Fragments arranged by size:
6081 1378 357 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3481 1186
Size: 2295 5806
BseYI C'CCAG_C
Cuts at: 7304 7800 7304
Size: 496 7605
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5551 6312 7340 1540
Size: 275 186 3550 761 1028 2301
Fragments arranged by size:
3550 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4032 4104 4371 6162 6247 7318 4032
Size: 72 267 1791 85 1071 4815
Fragments arranged by size:
4815 1791 1071 267 85 72
BsmI GAATG_Cn'
Cuts at: 3287 5612 5705 3287
Size: 2325 93 5683
Fragments arranged by size:
5683 2325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3125 3181 4032 4104 4371 4877 5420
Size: 399 1335 56 851 72 267 506 543
Cuts at: 5420 6162 6247 7318 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1335 1071 851 742 543 506 399
267 85 72 56
BspEI T'CCGG_A
Cuts at: 7764 7764
Size: 8101
BspHI T'CATG_A
Cuts at: 3739 5990 7720 3739
Size: 2251 1730 4120
Fragments arranged by size:
4120 2251 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5456 844
Size: 4612 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5988 1394
Size: 429 4165 3507
Fragments arranged by size:
4165 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 2592 3786 4861 6596 6770 65
Size: 1183 870 474 1194 1075 1735 174 1396
Fragments arranged by size:
1735 1396 1194 1183 1075 870 474 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6745 6932 1700
Size: 110 4935 187 2869
Fragments arranged by size:
4935 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2245 2603 4633 4743 96
Size: 2149 358 2030 110 3454
Fragments arranged by size:
3454 2149 2030 358 110
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8101
BssSI C'ACGA_G
Cuts at: 6161 7173 6161
Size: 1012 7089
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8101
BstBI TT'CG_AA
Cuts at: 6905 6905
Size: 8101
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8101
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2728 5215 2011
Size: 717 2487 4897
Fragments arranged by size:
4897 2487 717
BstYI r'GATC_y
Cuts at: 1972 4825 5431 5798 6183 6200 7652 7738 7750
Size: 2853 606 367 385 17 1452 86 12
Cuts at: 7750 8096 1972
Size: 346 1977
Fragments arranged by size:
2853 1977 1452 606 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2163 4981 2163
Size: 2818 5283
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7362
Fragments arranged by size:
7362 683 56
BtsI GCAGTG_nn'
Cuts at: 3948 4371 5617 6411 6431 3948
Size: 423 1246 794 20 5618
Fragments arranged by size:
5618 1246 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 2619 4153 1554
Size: 1065 1534 5502
Fragments arranged by size:
5502 1534 1065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3937 3972 4662 4697 529
Size: 35 3373 35 690 35 3933
Fragments arranged by size:
3933 3373 690 35 35 35
DraI TTT'AAA
Cuts at: 3027 4225 4428 5405 3027
Size: 1198 203 977 5723
Fragments arranged by size:
5723 1198 977 203
DraIII CAC_nnn'GTG
Cuts at: 3803 5086 3803
Size: 1283 6818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2369 7108 809
Size: 507 1053 4739 1802
Fragments arranged by size:
4739 1802 1053 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 5548 6437
Size: 54 1425 50 275 18 1465 2253 889
Cuts at: 6437 8066 8
Size: 1629 43
Fragments arranged by size:
2253 1629 1465 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 3384 3499 5367 6031 1353
Size: 693 1338 115 1868 664 3423
Fragments arranged by size:
3423 1868 1338 693 664 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5329 6688 7062 7208 387
Size: 1064 138 3740 1359 374 146 1280
Fragments arranged by size:
3740 1359 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4102 4102
Size: 8101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2067 3810 5060 5334 6164 6761
Size: 393 381 54 1743 1250 274 830 597
Cuts at: 6761 7548 7816 1239
Size: 787 268 1524
Fragments arranged by size:
1743 1524 1250 830 787 597 393 381
274 268 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2246
Size: 8101
EcoO109I rG'GnC_Cy
Cuts at: 2732 4874 2732
Size: 2142 5959
EcoRI G'AATT_C
Cuts at: 3272 5410 5788 3272
Size: 2138 378 5585
Fragments arranged by size:
5585 2138 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5065 5065
Size: 8101
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 7248 1678
Size: 5570 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 3032 3064 3320
Size: 32 135 32 968 32 460 32 256
Cuts at: 3320 3352 4162 4194 4228 4260 4810 4842 5321
Size: 32 810 32 34 32 550 32 479
Cuts at: 5321 5337 5353 5369 6742 6774 6816 6848 1373
Size: 16 16 16 1373 32 42 32 2626
Fragments arranged by size:
2626 1373 968 810 550 479 460 256
135 42 34 32 32 32 32 32
32 32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 3187 3753 5445 5691 669
Size: 988 281 436 813 566 1692 246 3079
Fragments arranged by size:
3079 1692 988 813 566 436 281 246
HindIII A'AGCT_T
Cuts at: 748 2112 4219 5042 748
Size: 1364 2107 823 3807
Fragments arranged by size:
3807 2107 1364 823
HpaI GTT'AAC
Cuts at: 3187 3753 5691 3187
Size: 566 1938 5597
Fragments arranged by size:
5597 1938 566
KpnI G_GTAC'C
Cuts at: 5426 5426
Size: 8101
MfeI C'AATT_G
Cuts at: 2560 2637 5700 2560
Size: 77 3063 4961
Fragments arranged by size:
4961 3063 77
MluI A'CGCG_T
Cuts at: 7977 7977
Size: 8101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4358 7215 7399 1305
Size: 3053 2857 184 2007
Fragments arranged by size:
3053 2857 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3297 10
Size: 54 1425 343 1465 4814
Fragments arranged by size:
4814 1465 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2807 6059 6418 6577 518
Size: 648 843 798 3252 359 159 2042
Fragments arranged by size:
3252 2042 843 798 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3473 5517 6194 7342 7587 1606
Size: 1867 2044 677 1148 245 2120
Fragments arranged by size:
2120 2044 1867 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7549
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8101
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NspI r_CATG'y
Cuts at: 1132 1229 4007 4068 5459 7004 1132
Size: 97 2778 61 1391 1545 2229
Fragments arranged by size:
2778 2229 1545 1391 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8101
PciI A'CATG_T
Cuts at: 7000 7000
Size: 8101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8101
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6170 6202 7683 7715 6170
Size: 32 1481 32 6556
Fragments arranged by size:
6556 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 2732
Size: 8101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8101
PsiI TTA'TAA
Cuts at: 2309 5671 2309
Size: 3362 4739
PspOMI G'GGCC_C
Cuts at: 4873 4873
Size: 8101
PstI C_TGCA'G
Cuts at: 830 2926 3955 3985 4366 5453 830
Size: 2096 1029 30 381 1087 3478
Fragments arranged by size:
3478 2096 1087 1029 381 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8101
PvuII CAG'CTG
Cuts at: 3473 3473
Size: 8101
SacI G_AGCT'C
Cuts at: 4104 4104
Size: 8101
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SapI GCTCTTCn'nnn_
Cuts at: 3384 3384
Size: 8101
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 4213 4640 6349 8061 4213
Size: 427 1709 1712 4253
Fragments arranged by size:
4253 1712 1709 427
SfcI C'TryA_G
Cuts at: 826 1045 2535 2695 2922 3426 3462 3951 3981
Size: 219 1490 160 227 504 36 489 30
Cuts at: 3981 4362 4780 4865 5314 5449 6584 7265 7456
Size: 381 418 85 449 135 1135 681 191
Cuts at: 7456 826
Size: 1471
Fragments arranged by size:
1490 1471 1135 681 504 489 449 418
381 227 219 191 160 135 85 36
30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 5428 1872
Size: 3556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 3318 3672 5156 6205 7106
Size: 197 926 765 610 354 1484 1049 901
Cuts at: 7106 7368 820
Size: 262 1553
Fragments arranged by size:
1553 1484 1049 926 901 765 610 354
262 197
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 4007 5459 1132
Size: 2875 1452 3774
Fragments arranged by size:
3774 2875 1452
SspI AAT'ATT
Cuts at: 5 52 5235 6025 5
Size: 47 5183 790 2081
Fragments arranged by size:
5183 2081 790 47
StuI AGG'CCT
Cuts at: 1517 4014 1517
Size: 2497 5604
StyI C'CwwG_G
Cuts at: 513 1065 2836 2978 5049 5540 513
Size: 552 1771 142 2071 491 3074
Fragments arranged by size:
3074 2071 1771 552 491 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4015 6128 6145 6298 6483 6928 325
Size: 1255 2435 2113 17 153 185 445 1498
Fragments arranged by size:
2435 2113 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2245 2458 2603
Size: 274 80 33 51 1443 268 213 145
Cuts at: 2603 4211 4358 4633 4638 4743 6347 8059 96
Size: 1608 147 275 5 105 1604 1712 138
Fragments arranged by size:
1712 1608 1604 1443 275 274 268 213
147 145 138 105 80 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3787 3819 8061 8093 3787
Size: 32 4242 32 3795
Fragments arranged by size:
4242 3795 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8101
XbaI T'CTAG_A
Cuts at: 5437 5437
Size: 8101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4694 4867 4694
Size: 173 7928
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8101
XmaI C'CCGG_G
Cuts at: 1870 5426 1870
Size: 3556 4545
XmnI GAAnn'nnTTC
Cuts at: 2475 3615 6230 2475
Size: 1140 2615 4346
Fragments arranged by size:
4346 2615 1140
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5909 276
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI