Restriction map for
pFN21AE6792
(Circular) (Six-base) MAPSORT of: pFN21AE6792.seq Check: 4200 from: 1 to: 7075
pFN21AE6792 7075 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4885 278
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4418 1937
Size: 2481 4594
Acc65I G'GTAC_C
Cuts at: 4396 4396
Size: 7075
AclI AA'CG_TT
Cuts at: 1563 5202 5575 1563
Size: 3639 373 3063
Fragments arranged by size:
3639 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2510 2835 4284 5138 6522 6790 1632
Size: 495 383 325 1449 854 1384 268 1917
Fragments arranged by size:
1917 1449 1384 854 495 383 325 268
AflII C'TTAA_G
Cuts at: 820 1017 2072 2524 3200 820
Size: 197 1055 452 676 4695
Fragments arranged by size:
4695 1055 676 452 197
AflIII A'CryG_T
Cuts at: 1203 2473 2791 3164 3369 5974 6951 1203
Size: 1270 318 373 205 2605 977 1327
Fragments arranged by size:
2605 1327 1270 977 373 318 205
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 5804 5804
Size: 7075
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3327 3427 1184
Size: 2143 100 4832
Fragments arranged by size:
4832 2143 100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3412 3444 3412
Size: 32 7043
AlwNI CAG_nnn'CTG
Cuts at: 4818 6390 4818
Size: 1572 5503
ApaLI G'TGCA_C
Cuts at: 5132 6288 5132
Size: 1156 5919
ApoI r'AATT_y
Cuts at: 1493 3421 3624 3631 3655 4384 4610 4762 6871
Size: 1928 203 7 24 729 226 152 2109
Cuts at: 6871 1493
Size: 1697
Fragments arranged by size:
2109 1928 1697 729 226 203 152 24
7
AseI AT'TA_AT
Cuts at: 160 1032 4100 5629 160
Size: 872 3068 1529 1606
Fragments arranged by size:
3068 1606 1529 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 3924 4400 1870
Size: 73 1981 476 4545
Fragments arranged by size:
4545 1981 476 73
AvrII C'CTAG_G
Cuts at: 3811 3811
Size: 7075
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3358 3391 3358
Size: 33 7042
BamHI G'GATC_C
Cuts at: 2594 4405 4772 2594
Size: 1811 367 4897
Fragments arranged by size:
4897 1811 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3311 3830 3973 4396 5851
Size: 325 217 627 1524 519 143 423 1455
Cuts at: 5851 618
Size: 1842
Fragments arranged by size:
1842 1524 1455 627 519 423 325 217
143
BanII G_rGCy'C
Cuts at: 1391 2348 2448 3098 4394 1391
Size: 957 100 650 1296 4072
Fragments arranged by size:
4072 1296 957 650 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5266 5300 1903
Size: 34 3329 34 3678
Fragments arranged by size:
3678 3329 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2739 3359 4967 6177 2739
Size: 620 1608 1210 3637
Fragments arranged by size:
3637 1608 1210 620
BclI T'GATC_A
Cuts at: 1548 2638 3595 1548
Size: 1090 957 5028
Fragments arranged by size:
5028 1090 957
BfrBI ATG'CAT
Cuts at: 2391 2391
Size: 7075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3554 5686 136
Size: 107 122 71 631 605 1882 2132 1525
Fragments arranged by size:
2132 1882 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2019 7070 2019
Size: 5051 2024
BlpI GC'TnA_GC
Cuts at: 2150 4492 2150
Size: 2342 4733
Bme1580I G_kGCm'C
Cuts at: 1790 2884 3770 3976 5136 6292 1790
Size: 1094 886 206 1160 1156 2573
Fragments arranged by size:
2573 1160 1156 1094 886 206
BmgBI CAC'GTC
Cuts at: 1318 2224 1318
Size: 906 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2457 2715 3117
Size: 416 53 83 399 24 1040 258 402
Cuts at: 3117 4806 5764 442
Size: 1689 958 1753
Fragments arranged by size:
1753 1689 1040 958 416 402 399 258
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7075
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 4011 5735 1239
Size: 1085 1687 1724 2579
Fragments arranged by size:
2579 1724 1687 1085
Bpu10I CC'TnA_GC
Cuts at: 2579 3097 2579
Size: 518 6557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2661 5200 6065 6363 2435
Size: 226 2539 865 298 3147
Fragments arranged by size:
3147 2539 865 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2405 5738 882
Size: 617 906 3333 2219
Fragments arranged by size:
3333 2219 906 617
BsaAI yAC'GTr
Cuts at: 493 1204 3370 4866 493
Size: 711 2166 1496 2702
Fragments arranged by size:
2702 2166 1496 711
BsaBI GATnn'nnATC
Cuts at: 7069 7069
Size: 7075
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4882 5264 5899 275
Size: 53 83 186 936 3349 382 635 1451
Fragments arranged by size:
3349 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5508 5906 6180 6327 6738 5508
Size: 398 274 147 411 5845
Fragments arranged by size:
5845 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2330 2360 1178
Size: 30 165 30 927 30 5893
Fragments arranged by size:
5893 927 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3275 1186
Size: 2089 4986
BseYI C'CCAG_C
Cuts at: 3022 3762 6278 6774 3022
Size: 740 2516 496 3323
Fragments arranged by size:
3323 2516 740 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2189 3523 1163
Size: 573 300 153 1334 4715
Fragments arranged by size:
4715 1334 573 300 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 5286 6314 1540
Size: 275 186 2524 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5136 5221 6292 5136
Size: 85 1071 5919
Fragments arranged by size:
5919 1071 85
BsmI GAATG_Cn'
Cuts at: 4586 4679 4586
Size: 93 6982
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2448 2884 3098 3770 3976 4394
Size: 399 558 100 436 214 672 206 418
Cuts at: 4394 5136 5221 6292 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 558 436 418 399
214 206 100 85
BspEI T'CCGG_A
Cuts at: 6738 6738
Size: 7075
BspHI T'CATG_A
Cuts at: 3016 3340 4964 6694 3016
Size: 324 1624 1730 3397
Fragments arranged by size:
3397 1730 1624 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4430 844
Size: 3586 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4962 1394
Size: 429 3139 3507
Fragments arranged by size:
3507 3139 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5570 5744 65
Size: 1183 4322 174 1396
Fragments arranged by size:
4322 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5719 5906 1700
Size: 110 3909 187 2869
Fragments arranged by size:
3909 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4222 96
Size: 4126 2949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7075
BssSI C'ACGA_G
Cuts at: 5135 6147 5135
Size: 1012 6063
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7075
BstBI TT'CG_AA
Cuts at: 3628 5879 3628
Size: 2251 4824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7075
BstYI r'GATC_y
Cuts at: 1972 2019 2594 3506 4405 4772 5157 5174 6626
Size: 47 575 912 899 367 385 17 1452
Cuts at: 6626 6712 6724 7070 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 912 899 575 385 367 346
86 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3549 3795 513
Size: 552 2484 246 3793
Fragments arranged by size:
3793 2484 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6336
Fragments arranged by size:
6336 683 56
BtsI GCAGTG_nn'
Cuts at: 3527 3955 4591 5385 5405 3527
Size: 428 636 794 20 5197
Fragments arranged by size:
5197 794 636 428 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7040
DraI TTT'AAA
Cuts at: 3142 3637 3862 4379 3142
Size: 495 225 517 5838
Fragments arranged by size:
5838 517 495 225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3336 6082 809
Size: 507 2020 2746 1802
Fragments arranged by size:
2746 2020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4522 5411 7040
Size: 54 1425 50 275 18 2692 889 1629
Cuts at: 7040 8
Size: 43
Fragments arranged by size:
2692 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3157 5005 1353
Size: 1804 1848 3423
Fragments arranged by size:
3423 1848 1804
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5662 6036 6182 387
Size: 1064 138 4073 374 146 1280
Fragments arranged by size:
4073 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2324 2510 2835 4011 4284 5138
Size: 393 495 197 186 325 1176 273 854
Cuts at: 5138 5735 6522 6790 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1176 854 787 597 495 393 325
273 268 197 186
EcoNI CCTnn'n_nnAGG
Cuts at: 3810 3810
Size: 7075
EcoRI G'AATT_C
Cuts at: 3421 3624 4384 4762 3421
Size: 203 760 378 5734
Fragments arranged by size:
5734 760 378 203
EcoRV GAT'ATC
Cuts at: 2801 2801
Size: 7075
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 7075
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 6222 1678
Size: 4544 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3818 3850 5716 5748 5790
Size: 32 135 32 2246 32 1866 32 42
Cuts at: 5790 5822 1373
Size: 32 2626
Fragments arranged by size:
2626 2246 1866 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3583 4419 4665 669
Size: 988 281 1645 836 246 3079
Fragments arranged by size:
3079 1645 988 836 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7075
HpaI GTT'AAC
Cuts at: 3583 4665 3583
Size: 1082 5993
KpnI G_GTAC'C
Cuts at: 4400 4400
Size: 7075
MfeI C'AATT_G
Cuts at: 3118 4674 3118
Size: 1556 5519
MluI A'CGCG_T
Cuts at: 2791 6951 2791
Size: 4160 2915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2084 2776 6189 6373 1305
Size: 779 692 3413 184 2007
Fragments arranged by size:
3413 2007 779 692 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5253
Fragments arranged by size:
5253 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 4079 5033 5392 5551 518
Size: 648 1224 1689 954 359 159 2042
Fragments arranged by size:
2042 1689 1224 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4491 5168 6316 6561 1606
Size: 2885 677 1148 245 2120
Fragments arranged by size:
2885 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3549 3795 513
Size: 552 2484 246 3793
Fragments arranged by size:
3793 2484 552 246
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7075
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7075
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NsiI A_TGCA'T
Cuts at: 2393 2393
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 2391 2477 3168 3964 4433 5978 1132
Size: 97 1162 86 691 796 469 1545 2229
Fragments arranged by size:
2229 1545 1162 796 691 469 97 86
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7075
PciI A'CATG_T
Cuts at: 2473 3164 5974 2473
Size: 691 2810 3574
Fragments arranged by size:
3574 2810 691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7075
PfoI T'CCnGG_A
Cuts at: 2590 4027 2590
Size: 1437 5638
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5144 5176 6657 6689 5144
Size: 32 1481 32 5530
Fragments arranged by size:
5530 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7075
PsiI TTA'TAA
Cuts at: 3450 4645 3450
Size: 1195 5880
PstI C_TGCA'G
Cuts at: 830 2205 3894 4427 830
Size: 1375 1689 533 3478
Fragments arranged by size:
3478 1689 1375 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 2615 2971 3410 5323 7035 2615
Size: 356 439 1913 1712 2655
Fragments arranged by size:
2655 1913 1712 439 356
SexAI A'CCwGG_T
Cuts at: 3005 3307 3005
Size: 302 6773
SfcI C'TryA_G
Cuts at: 826 1045 2201 3033 3319 3674 3890 4423 5558
Size: 219 1156 832 286 355 216 533 1135
Cuts at: 5558 6239 6430 826
Size: 681 191 1471
Fragments arranged by size:
1471 1156 1135 832 681 533 355 286
219 216 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7075
SmaI CCC'GGG
Cuts at: 1872 3926 4402 1872
Size: 2054 476 4545
Fragments arranged by size:
4545 2054 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 2450 2524 2676 3200 5179
Size: 197 926 129 378 74 152 524 1979
Cuts at: 5179 6080 6342 820
Size: 901 262 1553
Fragments arranged by size:
1979 1553 926 901 524 378 262 197
152 129 74
SnaBI TAC'GTA
Cuts at: 493 4866 493
Size: 4373 2702
SpeI A'CTAG_T
Cuts at: 152 2053 152
Size: 1901 5174
SphI G_CATG'C
Cuts at: 1132 4433 1132
Size: 3301 3774
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 2456 1517
Size: 939 6136
StyI C'CwwG_G
Cuts at: 513 1065 3549 3795 3811 4514 513
Size: 552 2484 246 16 703 3074
Fragments arranged by size:
3074 2484 703 552 246 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2622 3957 5102 5119 5272 5457 5902
Size: 1255 1042 1335 1145 17 153 185 445
Cuts at: 5902 325
Size: 1498
Fragments arranged by size:
1498 1335 1255 1145 1042 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 2969 3408
Size: 274 80 33 51 1443 636 356 439
Cuts at: 3408 4222 5321 7033 96
Size: 814 1099 1712 138
Fragments arranged by size:
1712 1443 1099 814 636 439 356 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7035 7067 7035
Size: 32 7043
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7075
XbaI T'CTAG_A
Cuts at: 2947 4411 2947
Size: 1464 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3533 3793 3962 3533
Size: 260 169 6646
Fragments arranged by size:
6646 260 169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7075
XmaI C'CCGG_G
Cuts at: 1870 3924 4400 1870
Size: 2054 476 4545
Fragments arranged by size:
4545 2054 476
XmnI GAAnn'nnTTC
Cuts at: 5204 5204
Size: 7075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4883 276
Size: 53 83 186 936 3349 2468
Fragments arranged by size:
3349 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI