Restriction map for
pFN21AE6791
(Circular) (Six-base) MAPSORT of: pFN21AE6791.seq Check: 4776 from: 1 to: 7033
pFN21AE6791 7033 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4843 278
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3550 4376 1937
Size: 1613 826 4594
Fragments arranged by size:
4594 1613 826
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 2862 5160 5533 1563
Size: 1299 2298 373 3063
Fragments arranged by size:
3063 2298 1299 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 3395 5096 6480 6748 1632
Size: 1124 639 1701 1384 268 1917
Fragments arranged by size:
1917 1701 1384 1124 639 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 3674 4285 5932 6909 1203
Size: 2471 611 1647 977 1327
Fragments arranged by size:
2471 1647 1327 977 611
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 4243 1166
Size: 3077 3956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3545 1184
Size: 2361 4672
AlwNI CAG_nnn'CTG
Cuts at: 4776 6348 4776
Size: 1572 5461
ApaLI G'TGCA_C
Cuts at: 2177 5090 6246 2177
Size: 2913 1156 2964
Fragments arranged by size:
2964 2913 1156
ApoI r'AATT_y
Cuts at: 1493 3278 3524 4342 4568 4720 6829 1493
Size: 1785 246 818 226 152 2109 1697
Fragments arranged by size:
2109 1785 1697 818 246 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 4358 1870
Size: 73 2415 4545
Fragments arranged by size:
4545 2415 73
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4129 4354 5809 618
Size: 325 217 627 2342 225 1455 1842
Fragments arranged by size:
2342 1842 1455 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2172 4352 1391
Size: 781 2180 4072
Fragments arranged by size:
4072 2180 781
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3147 928
Size: 971 1248 4814
Fragments arranged by size:
4814 1248 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 2298 1548
Size: 750 6283
BfrBI ATG'CAT
Cuts at: 3253 3253
Size: 7033
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4008 7028 4008
Size: 3020 4013
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 2181 3935 4132 5094 6250 1790
Size: 391 1754 197 962 1156 2573
Fragments arranged by size:
2573 1754 1156 962 391 197
BmgBI CAC'GTC
Cuts at: 1318 3675 1318
Size: 2357 4676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2251 3211 4764
Size: 416 53 83 399 24 834 960 1553
Cuts at: 4764 5722 442
Size: 958 1753
Fragments arranged by size:
1753 1553 960 958 834 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3750 3750
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 2435 3144 5693 1239
Size: 1017 179 709 2549 2579
Fragments arranged by size:
2579 2549 1017 709 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5158 6023 6321 5158
Size: 865 298 5870
Fragments arranged by size:
5870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2958 5696 882
Size: 617 1459 2738 2219
Fragments arranged by size:
2738 2219 1459 617
BsaAI yAC'GTr
Cuts at: 493 1204 4824 493
Size: 711 3620 2702
Fragments arranged by size:
3620 2702 711
BsaBI GATnn'nnATC
Cuts at: 4013 7027 4013
Size: 3014 4019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4840 5222 5857 275
Size: 53 83 186 936 3307 382 635 1451
Fragments arranged by size:
3307 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5466 5864 6138 6285 6696 5466
Size: 398 274 147 411 5803
Fragments arranged by size:
5803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3117 3147 3623 3653 1178
Size: 30 165 30 1714 30 476 30 4558
Fragments arranged by size:
4558 1714 476 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2094 1186
Size: 908 6125
BseYI C'CCAG_C
Cuts at: 3927 6236 6732 3927
Size: 2309 496 4228
Fragments arranged by size:
4228 2309 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2391 2864 3647 3917 1163
Size: 573 655 473 783 270 4279
Fragments arranged by size:
4279 783 655 573 473 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 5244 6272 1540
Size: 275 186 2482 761 1028 2301
Fragments arranged by size:
2482 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 5094 5179 6250 2181
Size: 2913 85 1071 2964
Fragments arranged by size:
2964 2913 1071 85
BsmI GAATG_Cn'
Cuts at: 2562 3658 3886 4544 4637 2562
Size: 1096 228 658 93 4958
Fragments arranged by size:
4958 1096 658 228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2181 3935 4132 4352 5094 5179
Size: 399 382 9 1754 197 220 742 85
Cuts at: 5179 6250 1391
Size: 1071 2174
Fragments arranged by size:
2174 1754 1071 742 399 382 220 197
85 9
BspEI T'CCGG_A
Cuts at: 6696 6696
Size: 7033
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4079 4388 844
Size: 3235 309 3489
Fragments arranged by size:
3489 3235 309
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3595 5528 5702 65
Size: 1183 2347 1933 174 1396
Fragments arranged by size:
2347 1933 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 3179 5093 6105 3179
Size: 1914 1012 4107
Fragments arranged by size:
4107 1914 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 3519 3728 794
Size: 1652 1073 209 4099
Fragments arranged by size:
4099 1652 1073 209
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstXI CCAn_nnnn'nTGG
Cuts at: 3185 3185
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 2029 2037 3448 3636 4008 4363 4730 5115
Size: 57 8 1411 188 372 355 367 385
Cuts at: 5115 5132 6584 6670 6682 7028 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1411 385 372 367 355 346
188 86 57 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 2488 513
Size: 552 939 484 5058
Fragments arranged by size:
5058 939 552 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2166 3443 4111 4549 5343 5363 2100
Size: 66 1277 668 438 794 20 3770
Fragments arranged by size:
3770 1277 794 668 438 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DraIII CAC_nnn'GTG
Cuts at: 3219 3219
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 3712 4480
Size: 54 1425 50 275 18 403 1479 768
Cuts at: 4480 5369 6998 8
Size: 889 1629 43
Fragments arranged by size:
1629 1479 1425 889 768 403 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2119 2199 2679 4963 1353
Size: 766 80 480 2284 3423
Fragments arranged by size:
3423 2284 766 480 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2435 2756 3144 3395 5096 5693
Size: 393 624 179 321 388 251 1701 597
Cuts at: 5693 6480 6748 1239
Size: 787 268 1524
Fragments arranged by size:
1701 1524 787 624 597 393 388 321
268 251 179
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 2450
Size: 7033
EcoO109I rG'GnC_Cy
Cuts at: 2250 2506 2250
Size: 256 6777
EcoRI G'AATT_C
Cuts at: 4342 4720 4342
Size: 378 6655
EcoRV GAT'ATC
Cuts at: 2813 3829 2813
Size: 1016 6017
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 3586 3618 3623
Size: 32 135 32 786 32 1196 32 5
Cuts at: 3623 3655 5674 5706 5748 5780 1373
Size: 32 2019 32 42 32 2626
Fragments arranged by size:
2626 2019 1196 786 135 42 32 32
32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 4377 4623 669
Size: 988 281 741 1698 246 3079
Fragments arranged by size:
3079 1698 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 3074 748
Size: 2326 4707
HpaI GTT'AAC
Cuts at: 2679 4623 2679
Size: 1944 5089
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 2714 4632 2714
Size: 1918 5115
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3797 6147 6331 1305
Size: 2492 2350 184 2007
Fragments arranged by size:
2492 2350 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2235 3714 10
Size: 54 1425 343 403 1479 3329
Fragments arranged by size:
3329 1479 1425 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2493 3912 4243 4991 5350 5509 518
Size: 648 1327 1419 331 748 359 159 2042
Fragments arranged by size:
2042 1419 1327 748 648 359 331 159
MspA1I CmG'CkG
Cuts at: 1606 2773 4449 5126 6274 6519 1606
Size: 1167 1676 677 1148 245 2120
Fragments arranged by size:
2120 1676 1167 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2488 513
Size: 552 939 484 5058
Fragments arranged by size:
5058 939 552 484
NdeI CA'TA_TG
Cuts at: 387 3805 387
Size: 3418 3615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NsiI A_TGCA'T
Cuts at: 3255 3255
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 2444 3257 4289 4391 5936 1132
Size: 97 1215 813 1032 102 1545 2229
Fragments arranged by size:
2229 1545 1215 1032 813 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 4285 5932 4285
Size: 1647 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3927 1108
Size: 2819 4214
PfoI T'CCnGG_A
Cuts at: 2326 2451 2469 2326
Size: 125 18 6890
Fragments arranged by size:
6890 125 18
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2506 2506
Size: 7033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 3009 4603 3009
Size: 1594 5439
PstI C_TGCA'G
Cuts at: 830 3293 4385 830
Size: 2463 1092 3478
Fragments arranged by size:
3478 2463 1092
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SanDI GG'GwC_CC
Cuts at: 2506 2506
Size: 7033
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 3316 3380 5281 6993 3316
Size: 64 1901 1712 3356
Fragments arranged by size:
3356 1901 1712 64
SfcI C'TryA_G
Cuts at: 826 1045 2341 2561 3236 3259 3289 3891 4381
Size: 219 1296 220 675 23 30 602 490
Cuts at: 4381 5516 6197 6388 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1296 1135 681 675 602 490 220
219 191 30 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5137 6038 6300 820
Size: 197 926 3194 901 262 1553
Fragments arranged by size:
3194 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 2444 3257 4391 1132
Size: 1312 813 1134 3774
Fragments arranged by size:
3774 1312 1134 813
SspI AAT'ATT
Cuts at: 5 52 2806 3305 4957 5
Size: 47 2754 499 1652 2081
Fragments arranged by size:
2754 2081 1652 499 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2004 2488 2517 3763 4472 513
Size: 552 939 484 29 1246 709 3074
Fragments arranged by size:
3074 1246 939 709 552 484 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 3230 3767 3776 5060 5077 5230
Size: 1255 573 1077 537 9 1284 17 153
Cuts at: 5230 5415 5860 325
Size: 185 445 1498
Fragments arranged by size:
1498 1284 1255 1077 573 537 445 185
153 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3314 3378 3470
Size: 274 80 33 51 1443 1337 64 92
Cuts at: 3470 5279 6991 96
Size: 1809 1712 138
Fragments arranged by size:
1809 1712 1443 1337 274 138 92 80
64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4197 4229 6993 7025 4197
Size: 32 2764 32 4205
Fragments arranged by size:
4205 2764 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 2122 4369 2122
Size: 2247 4786
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 3656 5162 3656
Size: 1506 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4841 276
Size: 53 83 186 936 3307 2468
Fragments arranged by size:
3307 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI