Restriction map for
pFN21AE6787
(Circular) (Six-base) MAPSORT of: pFN21AE6787.seq Check: 8387 from: 1 to: 6778
pFN21AE6787 6778 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4588 278
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4121 1937
Size: 2184 4594
Acc65I G'GTAC_C
Cuts at: 4099 4099
Size: 6778
AclI AA'CG_TT
Cuts at: 1563 4905 5278 1563
Size: 3342 373 3063
Fragments arranged by size:
3342 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4841 6225 6493 1632
Size: 3209 1384 268 1917
Fragments arranged by size:
3209 1917 1384 268
AfeI AGC'GCT
Cuts at: 3162 3162
Size: 6778
AflII C'TTAA_G
Cuts at: 820 1017 3781 820
Size: 197 2764 3817
Fragments arranged by size:
3817 2764 197
AflIII A'CryG_T
Cuts at: 1203 5677 6654 1203
Size: 4474 977 1327
Fragments arranged by size:
4474 1327 977
AgeI A'CCGG_T
Cuts at: 5609 5609
Size: 6778
AhdI GACnn_n'nnGTC
Cuts at: 5507 5507
Size: 6778
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3678 1184
Size: 2494 4284
AlwNI CAG_nnn'CTG
Cuts at: 3433 3914 4521 6093 3433
Size: 481 607 1572 4118
Fragments arranged by size:
4118 1572 607 481
ApaLI G'TGCA_C
Cuts at: 4835 5991 4835
Size: 1156 5622
ApoI r'AATT_y
Cuts at: 1493 2305 2426 3305 3820 4087 4313 4465 6574
Size: 812 121 879 515 267 226 152 2109
Cuts at: 6574 1493
Size: 1697
Fragments arranged by size:
2109 1697 879 812 515 267 226 152
121
AseI AT'TA_AT
Cuts at: 160 1032 3356 5332 160
Size: 872 2324 1976 1606
Fragments arranged by size:
2324 1976 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
AvaI C'yCGr_G
Cuts at: 1870 1943 4103 1870
Size: 73 2160 4545
Fragments arranged by size:
4545 2160 73
AvrII C'CTAG_G
Cuts at: 4024 4024
Size: 6778
BamHI G'GATC_C
Cuts at: 4108 4475 4108
Size: 367 6411
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2223 4099 5554 618
Size: 325 217 627 251 185 1876 1455 1842
Fragments arranged by size:
1876 1842 1455 627 325 251 217 185
BanII G_rGCy'C
Cuts at: 1391 3146 4097 1391
Size: 1755 951 4072
Fragments arranged by size:
4072 1755 951
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3809 928
Size: 971 1910 3897
Fragments arranged by size:
3897 1910 971
BbvCI CC'TCA_GC
Cuts at: 2092 3038 2092
Size: 946 5832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4969 5003 1903
Size: 34 3032 34 3678
Fragments arranged by size:
3678 3032 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2041 4670 5880 2041
Size: 2629 1210 2939
Fragments arranged by size:
2939 2629 1210
BclI T'GATC_A
Cuts at: 1548 2637 2857 1548
Size: 1089 220 5469
Fragments arranged by size:
5469 1089 220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5389 136
Size: 107 122 71 631 605 3717 1525
Fragments arranged by size:
3717 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2385 6773 2385
Size: 4388 2390
BlpI GC'TnA_GC
Cuts at: 4195 4195
Size: 6778
Bme1580I G_kGCm'C
Cuts at: 1790 2228 3453 4839 5995 1790
Size: 438 1225 1386 1156 2573
Fragments arranged by size:
2573 1386 1225 1156 438
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6778
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2229 3149 4509
Size: 416 53 83 399 24 812 920 1360
Cuts at: 4509 5467 442
Size: 958 1753
Fragments arranged by size:
1753 1360 958 920 812 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2364 2364
Size: 6778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2639 3116 5438 1239
Size: 1080 320 477 2322 2579
Fragments arranged by size:
2579 2322 1080 477 320
Bpu10I CC'TnA_GC
Cuts at: 2092 3038 2092
Size: 946 5832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 4903 5768 6066 2170
Size: 2733 865 298 2882
Fragments arranged by size:
2882 2733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5441 882
Size: 617 3942 2219
Fragments arranged by size:
3942 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3454 4569 493
Size: 711 2250 1115 2702
Fragments arranged by size:
2702 2250 1115 711
BsaBI GATnn'nnATC
Cuts at: 2259 3731 6772 2259
Size: 1472 3041 2265
Fragments arranged by size:
3041 2265 1472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4585 4967 5602 275
Size: 53 83 186 936 3052 382 635 1451
Fragments arranged by size:
3052 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2013 5211 5609 5883 6030 6441 2013
Size: 3198 398 274 147 411 2350
Fragments arranged by size:
3198 2350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6553
Fragments arranged by size:
6553 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2759 3026 3200 1186
Size: 1062 511 267 174 4764
Fragments arranged by size:
4764 1062 511 267 174
BseYI C'CCAG_C
Cuts at: 3151 3917 5981 6477 3151
Size: 766 2064 496 3452
Fragments arranged by size:
3452 2064 766 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6205
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4228 4989 6017 1540
Size: 275 186 2227 761 1028 2301
Fragments arranged by size:
2301 2227 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 4839 4924 5995 3780
Size: 1059 85 1071 4563
Fragments arranged by size:
4563 1071 1059 85
BsiWI C'GTAC_G
Cuts at: 2732 2732
Size: 6778
BsmI GAATG_Cn'
Cuts at: 2250 4289 4382 2250
Size: 2039 93 4646
Fragments arranged by size:
4646 2039 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 3146 3453 3780 4097 4839 4924
Size: 399 438 918 307 327 317 742 85
Cuts at: 4924 5995 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 918 742 438 399 327 317
307 85
BspEI T'CCGG_A
Cuts at: 2013 6441 2013
Size: 4428 2350
BspHI T'CATG_A
Cuts at: 3630 3742 4667 6397 3630
Size: 112 925 1730 4011
Fragments arranged by size:
4011 1730 925 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2939 4133 844
Size: 1304 791 1194 3489
Fragments arranged by size:
3489 1304 1194 791
BsrBI CCG'CTC
Cuts at: 1394 1823 4665 1394
Size: 429 2842 3507
Fragments arranged by size:
3507 2842 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5273 5447 65
Size: 1183 4025 174 1396
Fragments arranged by size:
4025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2131 5422 5609 1700
Size: 110 321 3291 187 2869
Fragments arranged by size:
3291 2869 321 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6778
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6778
BssSI C'ACGA_G
Cuts at: 4838 5850 4838
Size: 1012 5766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2265 794
Size: 1471 5307
BstBI TT'CG_AA
Cuts at: 5582 5582
Size: 6778
BstEII G'GTnAC_C
Cuts at: 1184 2162 1184
Size: 978 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2919 2919
Size: 6778
BstYI r'GATC_y
Cuts at: 1972 2385 3900 4108 4475 4860 4877 6329 6415
Size: 413 1515 208 367 385 17 1452 86
Cuts at: 6415 6427 6773 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1515 1452 413 385 367 346 208
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2453 3270 2453
Size: 817 5961
BtgI C'CryG_G
Cuts at: 513 1065 2113 2228 2886 3370 513
Size: 552 1048 115 658 484 3921
Fragments arranged by size:
3921 1048 658 552 484 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6039
Fragments arranged by size:
6039 683 56
BtsI GCAGTG_nn'
Cuts at: 2599 3912 4294 5088 5108 2599
Size: 1313 382 794 20 4269
Fragments arranged by size:
4269 1313 794 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3709 3744 529
Size: 35 3145 35 3563
Fragments arranged by size:
3563 3145 35 35
DraI TTT'AAA
Cuts at: 4082 4082
Size: 6778
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5785 809
Size: 507 4469 1802
Fragments arranged by size:
4469 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3225 4225 5114
Size: 54 1425 50 275 18 1395 1000 889
Cuts at: 5114 6743 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1395 1000 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4225 1537
Size: 275 2413 4090
Fragments arranged by size:
4090 2413 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2376 2884 4708 1353
Size: 1002 21 508 1824 3423
Fragments arranged by size:
3423 1824 1002 508 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2735 5365 5739 5885 387
Size: 1064 138 1146 2630 374 146 1280
Fragments arranged by size:
2630 1280 1146 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2639 3116 4841 5438 6225 6493
Size: 393 687 320 477 1725 597 787 268
Cuts at: 6493 1239
Size: 1524
Fragments arranged by size:
1725 1524 787 687 597 477 393 320
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2456 2144
Size: 312 6466
EcoO109I rG'GnC_Cy
Cuts at: 2974 3430 2974
Size: 456 6322
EcoRI G'AATT_C
Cuts at: 4087 4465 4087
Size: 378 6400
EcoRV GAT'ATC
Cuts at: 2503 2503
Size: 6778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2336 3016 2336
Size: 680 6098
FspI TGC'GCA
Cuts at: 5284 5284
Size: 6778
HaeII r_GCGC'y
Cuts at: 1678 3164 5925 1678
Size: 1486 2761 2531
Fragments arranged by size:
2761 2531 1486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 5419 5451 5493
Size: 32 135 32 503 32 3312 32 42
Cuts at: 5493 5525 1373
Size: 32 2626
Fragments arranged by size:
3312 2626 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4122 4368 669
Size: 988 281 2184 246 3079
Fragments arranged by size:
3079 2184 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6778
HpaI GTT'AAC
Cuts at: 4368 4368
Size: 6778
KpnI G_GTAC'C
Cuts at: 4103 4103
Size: 6778
MfeI C'AATT_G
Cuts at: 4377 4377
Size: 6778
MluI A'CGCG_T
Cuts at: 6654 6654
Size: 6778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 3618 5892 6076 1305
Size: 885 1428 2274 184 2007
Fragments arranged by size:
2274 2007 1428 885 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4956
Fragments arranged by size:
4956 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3180 3318 3815 4736 5095 5254 518
Size: 648 2014 138 497 921 359 159 2042
Fragments arranged by size:
2042 2014 921 648 497 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2950 3042 3211 3563 3878 4194 4871 6019
Size: 1344 92 169 352 315 316 677 1148
Cuts at: 6019 6264 1606
Size: 245 2120
Fragments arranged by size:
2120 1344 1148 677 352 316 315 245
169 92
NcoI C'CATG_G
Cuts at: 513 1065 2886 3370 513
Size: 552 1821 484 3921
Fragments arranged by size:
3921 1821 552 484
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6778
NotI GC'GGCC_GC
Cuts at: 4225 4225
Size: 6778
NspI r_CATG'y
Cuts at: 1132 1229 4136 5681 1132
Size: 97 2907 1545 2229
Fragments arranged by size:
2907 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6778
PciI A'CATG_T
Cuts at: 5677 5677
Size: 6778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 3308 1108
Size: 1443 757 4578
Fragments arranged by size:
4578 1443 757
PmeI GTTT'AAAC
Cuts at: 4082 4082
Size: 6778
PmlI CAC'GTG
Cuts at: 3454 3454
Size: 6778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4847 4879 6360 6392 4847
Size: 32 1481 32 5233
Fragments arranged by size:
5233 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6778
PsiI TTA'TAA
Cuts at: 3827 4348 3827
Size: 521 6257
PstI C_TGCA'G
Cuts at: 830 2571 4130 830
Size: 1741 1559 3478
Fragments arranged by size:
3478 1741 1559
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6778
PvuII CAG'CTG
Cuts at: 2950 3042 3211 3563 3878 2950
Size: 92 169 352 315 5850
Fragments arranged by size:
5850 352 315 169 92
SalI G'TCGA_C
Cuts at: 1936 4120 1936
Size: 2184 4594
SbfI CC_TGCA'GG
Cuts at: 4130 4130
Size: 6778
ScaI AGT'ACT
Cuts at: 2525 2794 5026 6738 2525
Size: 269 2232 1712 2565
Fragments arranged by size:
2565 2232 1712 269
SfcI C'TryA_G
Cuts at: 826 1045 2144 2567 2966 3109 3193 3573 4126
Size: 219 1099 423 399 143 84 380 553
Cuts at: 4126 5261 5942 6133 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 681 553 423 399 380
219 191 143 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6778
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6778
SmaI CCC'GGG
Cuts at: 1872 4105 1872
Size: 2233 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2185 3781 4882 5783 6045 820
Size: 197 926 242 1596 1101 901 262 1553
Fragments arranged by size:
1596 1553 1101 926 901 262 242 197
SnaBI TAC'GTA
Cuts at: 493 4569 493
Size: 4076 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6778
SphI G_CATG'C
Cuts at: 1132 4136 1132
Size: 3004 3774
SspI AAT'ATT
Cuts at: 5 52 4069 4702 5
Size: 47 4017 633 2081
Fragments arranged by size:
4017 2081 633 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6778
StyI C'CwwG_G
Cuts at: 513 1065 2886 3060 3370 3939 4024 4217 513
Size: 552 1821 174 310 569 85 193 3074
Fragments arranged by size:
3074 1821 569 552 310 193 174 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2184 2211 4805 4822 4975 5160
Size: 1255 446 158 27 2594 17 153 185
Cuts at: 5160 5605 325
Size: 445 1498
Fragments arranged by size:
2594 1498 1255 446 445 185 158 153
27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 2792 5024
Size: 274 80 33 51 1443 546 269 2232
Cuts at: 5024 6736 96
Size: 1712 138
Fragments arranged by size:
2232 1712 1443 546 274 269 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6738 6770 6738
Size: 32 6746
Tth111I GACn'n_nGTC
Cuts at: 1316 3238 1316
Size: 1922 4856
XbaI T'CTAG_A
Cuts at: 4114 4114
Size: 6778
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6778
XmaI C'CCGG_G
Cuts at: 1870 4103 1870
Size: 2233 4545
XmnI GAAnn'nnTTC
Cuts at: 3951 4907 3951
Size: 956 5822
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4586 276
Size: 53 83 186 936 3052 2468
Fragments arranged by size:
3052 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BfrBI BsmBI
BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI