Restriction map for
pFN21AE6786
(Circular) (Six-base) MAPSORT of: pFN21AE6786.seq Check: 4470 from: 1 to: 6592
pFN21AE6786 6592 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3370 3370
Size: 6592
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2465 3935 1937
Size: 528 1470 4594
Fragments arranged by size:
4594 1470 528
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2918 4655 6039 6307 1632
Size: 1286 1737 1384 268 1917
Fragments arranged by size:
1917 1737 1384 1286 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 3232 5491 6468 1203
Size: 2029 2259 977 1327
Fragments arranged by size:
2259 2029 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 2461 5321 2461
Size: 2860 3732
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2088 2390 2772 3707 1184
Size: 904 302 382 935 4069
Fragments arranged by size:
4069 935 904 382 302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 6560
AlwNI CAG_nnn'CTG
Cuts at: 2377 2504 2956 3107 3658 4335 5907 2377
Size: 127 452 151 551 677 1572 3062
Fragments arranged by size:
3062 1572 677 551 452 151 127
ApaI G_GGCC'C
Cuts at: 3065 3065
Size: 6592
ApaLI G'TGCA_C
Cuts at: 3250 3740 4649 5805 3250
Size: 490 909 1156 4037
Fragments arranged by size:
4037 1156 909 490
ApoI r'AATT_y
Cuts at: 1493 3782 3901 4127 4279 6388 1493
Size: 2289 119 226 152 2109 1697
Fragments arranged by size:
2289 2109 1697 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 2580 2998 3011 3065 3218 3674 3917
Size: 73 637 418 13 54 153 456 243
Cuts at: 3917 1870
Size: 4545
Fragments arranged by size:
4545 637 456 418 243 153 73 54
13
BamHI G'GATC_C
Cuts at: 3922 4289 3922
Size: 367 6225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3110 3581 3913 5368 618
Size: 325 217 627 1323 471 332 1455 1842
Fragments arranged by size:
1842 1455 1323 627 471 332 325 217
BanII G_rGCy'C
Cuts at: 1391 3033 3065 3080 3150 3911 1391
Size: 1642 32 15 70 761 4072
Fragments arranged by size:
4072 1642 761 70 32 15
BbeI G_GCGC'C
Cuts at: 3114 3114
Size: 6592
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 3655 928
Size: 971 522 1234 3865
Fragments arranged by size:
3865 1234 971 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 3434 3468 4783 4817 1903
Size: 34 624 34 839 34 1315 34 3678
Fragments arranged by size:
3678 1315 839 624 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3579 5203 136
Size: 107 122 71 631 605 1907 1624 1525
Fragments arranged by size:
1907 1624 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 3164 4009 3164
Size: 845 5747
Bme1580I G_kGCm'C
Cuts at: 1790 2655 3065 3254 3584 3744 4653 5809 1790
Size: 865 410 189 330 160 909 1156 2573
Fragments arranged by size:
2573 1156 909 865 410 330 189 160
BmgBI CAC'GTC
Cuts at: 1318 2170 2463 1318
Size: 852 293 5447
Fragments arranged by size:
5447 852 293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4323 5281 442
Size: 416 53 83 399 24 2906 958 1753
Fragments arranged by size:
2906 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2156 2837 2933 3134 3253 3845 5252
Size: 869 48 681 96 201 119 592 1407
Cuts at: 5252 1239
Size: 2579
Fragments arranged by size:
2579 1407 869 681 592 201 119 96
48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 4717 5582 5880 2075
Size: 2642 865 298 2787
Fragments arranged by size:
2787 2642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3271 5255 882
Size: 617 1772 1984 2219
Fragments arranged by size:
2219 1984 1772 617
BsaAI yAC'GTr
Cuts at: 493 1204 4383 493
Size: 711 3179 2702
Fragments arranged by size:
3179 2702 711
BsaBI GATnn'nnATC
Cuts at: 2597 6586 2597
Size: 3989 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3111 4399 4781 5416
Size: 53 83 186 936 1578 1288 382 635
Cuts at: 5416 275
Size: 1451
Fragments arranged by size:
1578 1451 1288 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3876 5025 5423 5697 5844 6255 3876
Size: 1149 398 274 147 411 4213
Fragments arranged by size:
4213 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2877 2907 3818 3848 3855
Size: 30 165 30 1474 30 911 30 7
Cuts at: 3855 3866 3885 3896 1178
Size: 11 19 11 3874
Fragments arranged by size:
3874 1474 911 165 30 30 30 30
19 11 11 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2653 2656 2801 1186
Size: 1189 278 3 145 4977
Fragments arranged by size:
4977 1189 278 145 3
BseYI C'CCAG_C
Cuts at: 5795 6291 5795
Size: 496 6096
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2732 2942 3404 1163
Size: 573 414 582 210 462 4351
Fragments arranged by size:
4351 582 573 462 414 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3132 4042 4803 5831 1540
Size: 275 186 1131 910 761 1028 2301
Fragments arranged by size:
2301 1131 1028 910 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 3005 3254 3744 4653 4738 5809 2142
Size: 863 249 490 909 85 1071 2925
Fragments arranged by size:
2925 1071 909 863 490 249 85
BsmI GAATG_Cn'
Cuts at: 4103 4196 4103
Size: 93 6499
BsmBI CGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 6592
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2655 3005 3033 3065 3080 3150
Size: 399 352 513 350 28 32 15 70
Cuts at: 3150 3254 3584 3744 3911 4653 4738 5809 1391
Size: 104 330 160 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 352 350 330
167 160 104 85 70 32 28 15
BspEI T'CCGG_A
Cuts at: 6255 6255
Size: 6592
BspHI T'CATG_A
Cuts at: 3328 3636 4481 6211 3328
Size: 308 845 1730 3709
Fragments arranged by size:
3709 1730 845 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3370 3947 844
Size: 2526 577 3489
Fragments arranged by size:
3489 2526 577
BsrBI CCG'CTC
Cuts at: 1394 1823 2496 2947 3729 4479 1394
Size: 429 673 451 782 750 3507
Fragments arranged by size:
3507 782 750 673 451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5087 5261 65
Size: 1183 3839 174 1396
Fragments arranged by size:
3839 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5236 5423 1700
Size: 110 3426 187 2869
Fragments arranged by size:
3426 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6592
BstBI TT'CG_AA
Cuts at: 5396 5396
Size: 6592
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2326
Size: 6592
BstYI r'GATC_y
Cuts at: 1972 3537 3922 4289 4674 4691 6143 6229 6241
Size: 1565 385 367 385 17 1452 86 12
Cuts at: 6241 6587 1972
Size: 346 1977
Fragments arranged by size:
1977 1565 1452 385 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3114 513
Size: 552 2049 3991
Fragments arranged by size:
3991 2049 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3189 487
Size: 683 56 1963 3890
Fragments arranged by size:
3890 1963 683 56
BtsI GCAGTG_nn'
Cuts at: 4108 4902 4922 4108
Size: 794 20 5778
Fragments arranged by size:
5778 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DraIII CAC_nnn'GTG
Cuts at: 2808 2808
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2479 2644
Size: 54 1425 50 275 18 318 331 165
Cuts at: 2644 3117 3129 3186 4039 4928 6557 8
Size: 473 12 57 853 889 1629 43
Fragments arranged by size:
1629 1425 889 853 473 331 318 275
165 57 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3129 4039 1537
Size: 275 1317 910 4090
Fragments arranged by size:
4090 1317 910 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4522 1353
Size: 3169 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2732 3550 5179 5553 5699 387
Size: 1064 138 1143 818 1629 374 146 1280
Fragments arranged by size:
1629 1280 1143 1064 818 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2156 2837 2918 2933 3134 3253
Size: 393 476 48 681 81 15 201 119
Cuts at: 3253 3845 4655 5252 6039 6307 1239
Size: 592 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 681 597 592 476 393
268 201 119 81 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3203 3203
Size: 6592
EcoO109I rG'GnC_Cy
Cuts at: 3037 3062 3211 3723 3037
Size: 25 149 512 5906
Fragments arranged by size:
5906 512 149 25
EcoRI G'AATT_C
Cuts at: 3782 3901 4279 3782
Size: 119 378 6095
Fragments arranged by size:
6095 378 119
EcoRV GAT'ATC
Cuts at: 3437 3437
Size: 6592
FspI TGC'GCA
Cuts at: 2939 5098 2939
Size: 2159 4433
HaeII r_GCGC'y
Cuts at: 1678 3114 5739 1678
Size: 1436 2625 2531
Fragments arranged by size:
2625 2531 1436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2479 2877 2909 2959
Size: 32 135 32 875 32 398 32 50
Cuts at: 2959 2991 3529 3561 3866 3898 5233 5265 5307
Size: 32 538 32 305 32 1335 32 42
Cuts at: 5307 5339 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 875 538 398 305 135 50
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2253 2466 3874 3936 4182 669
Size: 988 281 315 213 1408 62 246 3079
Fragments arranged by size:
3079 1408 988 315 281 246 213 62
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 2253 4182 2253
Size: 1929 4663
KasI G'GCGC_C
Cuts at: 3110 3110
Size: 6592
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 3232 6468 3232
Size: 3236 3356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 3495 3627 5706 5890 1305
Size: 1329 861 132 2079 184 2007
Fragments arranged by size:
2079 2007 1329 861 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 2646 10
Size: 54 1425 343 649 165 3956
Fragments arranged by size:
3956 1425 649 343 165 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 3635 3764 4550 4909 5068 518
Size: 648 1588 881 129 786 359 159 2042
Fragments arranged by size:
2042 1588 881 786 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2374 2815 3141 3163 3171 4008 4685 5833
Size: 768 441 326 22 8 837 677 1148
Cuts at: 5833 6078 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 837 768 677 441 326 245
22 8
NarI GG'CG_CC
Cuts at: 3111 3111
Size: 6592
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6040
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NruI TCG'CGA
Cuts at: 2013 2013
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 3694 3763 3950 5495 1132
Size: 97 2465 69 187 1545 2229
Fragments arranged by size:
2465 2229 1545 187 97 69
PasI CC'CwG_GG
Cuts at: 1348 3859 1348
Size: 2511 4081
PciI A'CATG_T
Cuts at: 5491 5491
Size: 6592
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6592
PfoI T'CCnGG_A
Cuts at: 2949 2949
Size: 6592
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3211 3723 3211
Size: 512 6080
PshAI GACnn'nnGTC
Cuts at: 1613 3318 1613
Size: 1705 4887
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PspOMI G'GGCC_C
Cuts at: 3061 3061
Size: 6592
PstI C_TGCA'G
Cuts at: 830 3944 830
Size: 3114 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 2374 2815 3163 3171 2374
Size: 441 348 8 5795
Fragments arranged by size:
5795 441 348 8
SalI G'TCGA_C
Cuts at: 1936 2464 3934 1936
Size: 528 1470 4594
Fragments arranged by size:
4594 1470 528
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 4840 6552 4840
Size: 1712 4880
SexAI A'CCwGG_T
Cuts at: 2016 2016
Size: 6592
SfcI C'TryA_G
Cuts at: 826 1045 2989 3940 5075 5756 5947 826
Size: 219 1944 951 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 951 681 219 191
SfoI GGC'GCC
Cuts at: 3112 3112
Size: 6592
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3220 3676 3919 1872
Size: 1348 456 243 4545
Fragments arranged by size:
4545 1348 456 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 4696 5597 5859 820
Size: 197 926 147 2606 901 262 1553
Fragments arranged by size:
2606 1553 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 4383 493
Size: 3890 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3694 3950 1132
Size: 2562 256 3774
Fragments arranged by size:
3774 2562 256
SspI AAT'ATT
Cuts at: 5 52 4516 5
Size: 47 4464 2081
Fragments arranged by size:
4464 2081 47
StuI AGG'CCT
Cuts at: 1517 2987 1517
Size: 1470 5122
StyI C'CwwG_G
Cuts at: 513 1065 2226 4031 513
Size: 552 1161 1805 3074
Fragments arranged by size:
3074 1805 1161 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2556 3387 3719 3815 3863 4619 4636
Size: 1255 976 831 332 96 48 756 17
Cuts at: 4636 4789 4974 5419 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 976 831 756 445 332 185
153 96 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2711 4838 6550
Size: 274 80 33 51 1443 734 2127 1712
Cuts at: 6550 96
Size: 138
Fragments arranged by size:
2127 1712 1443 734 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 6552 6584 3818
Size: 32 2702 32 3826
Fragments arranged by size:
3826 2702 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2018 1316
Size: 702 5890
XbaI T'CTAG_A
Cuts at: 3421 3928 3421
Size: 507 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3218 3674 3917 1870
Size: 1348 456 243 4545
Fragments arranged by size:
4545 1348 456 243
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI
NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI XcmI