Restriction map for
pFN21AE6785
(Circular) (Six-base) MAPSORT of: pFN21AE6785.seq Check: 3070 from: 1 to: 6625
pFN21AE6785 6625 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2983 3537 2983
Size: 554 6071
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2632 3968 1937
Size: 695 1336 4594
Fragments arranged by size:
4594 1336 695
Acc65I G'GTAC_C
Cuts at: 2218 3946 2218
Size: 1728 4897
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3050 3787 4688 6072 6340 1632
Size: 1418 737 901 1384 268 1917
Fragments arranged by size:
1917 1418 1384 901 737 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 3120 5524 6501 1203
Size: 1917 2404 977 1327
Fragments arranged by size:
2404 1917 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 2621 2867 1166
Size: 1455 246 4924
Fragments arranged by size:
4924 1455 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2885 3022 3061 1184
Size: 1701 137 39 4748
Fragments arranged by size:
4748 1701 137 39
AlwNI CAG_nnn'CTG
Cuts at: 3439 4368 5940 3439
Size: 929 1572 4124
Fragments arranged by size:
4124 1572 929
ApaI G_GGCC'C
Cuts at: 2917 2917
Size: 6625
ApaLI G'TGCA_C
Cuts at: 2060 4682 5838 2060
Size: 2622 1156 2847
Fragments arranged by size:
2847 2622 1156
ApoI r'AATT_y
Cuts at: 1493 3934 4160 4312 6421 1493
Size: 2441 226 152 2109 1697
Fragments arranged by size:
2441 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 3138 3774 3950 1870
Size: 73 1195 636 176 4545
Fragments arranged by size:
4545 1195 636 176 73
BamHI G'GATC_C
Cuts at: 2334 3955 4322 2334
Size: 1621 367 4637
Fragments arranged by size:
4637 1621 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2218 2861 3515 3946
Size: 325 217 627 363 68 643 654 431
Cuts at: 3946 5401 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 654 643 627 431 363 325
217 68
BanII G_rGCy'C
Cuts at: 1391 2175 2668 2917 3063 3423 3944 1391
Size: 784 493 249 146 360 521 4072
Fragments arranged by size:
4072 784 521 493 360 249 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2973 928
Size: 971 1074 4580
Fragments arranged by size:
4580 1074 971
BbvCI CC'TCA_GC
Cuts at: 2654 2654
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2591 2625 4816 4850 1903
Size: 34 654 34 2191 34 3678
Fragments arranged by size:
3678 2191 654 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2255 4517 5727 2255
Size: 2262 1210 3153
Fragments arranged by size:
3153 2262 1210
BclI T'GATC_A
Cuts at: 1548 2163 2587 1548
Size: 615 424 5586
Fragments arranged by size:
5586 615 424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 5236 136
Size: 107 122 71 631 605 721 2843 1525
Fragments arranged by size:
2843 1525 721 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3451 3574 3885 6620 3451
Size: 123 311 2735 3456
Fragments arranged by size:
3456 2735 311 123
BlpI GC'TnA_GC
Cuts at: 3170 4042 3170
Size: 872 5753
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2153 2864 2917 3414 3520 3723 4686
Size: 274 89 711 53 497 106 203 963
Cuts at: 4686 5842 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 963 711 497 274 203 106
89 53
BmgBI CAC'GTC
Cuts at: 1318 3241 1318
Size: 1923 4702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3401 3747 4356
Size: 416 53 83 399 24 1984 346 609
Cuts at: 4356 5314 442
Size: 958 1753
Fragments arranged by size:
1984 1753 958 609 416 399 346 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2823 2823
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 2759 3716 5285 1239
Size: 1068 452 957 1569 2579
Fragments arranged by size:
2579 1569 1068 957 452
Bpu10I CC'TnA_GC
Cuts at: 2654 2654
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4750 5615 5913 4750
Size: 865 298 5462
Fragments arranged by size:
5462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 5288 882
Size: 617 861 2928 2219
Fragments arranged by size:
2928 2219 861 617
BsaAI yAC'GTr
Cuts at: 493 1204 4416 493
Size: 711 3212 2702
Fragments arranged by size:
3212 2702 711
BsaBI GATnn'nnATC
Cuts at: 6619 6619
Size: 6625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2241 3282 5058 5456 5730 5877 6288 2241
Size: 1041 1776 398 274 147 411 2578
Fragments arranged by size:
2578 1776 1041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 1178
Size: 30 165 30 820 30 5550
Fragments arranged by size:
5550 820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 3065 1186
Size: 1183 696 4746
Fragments arranged by size:
4746 1183 696
BseYI C'CCAG_C
Cuts at: 2038 2146 2223 2898 2976 3387 3559 3795 5828
Size: 108 77 675 78 411 172 236 2033
Cuts at: 5828 6324 2038
Size: 496 2339
Fragments arranged by size:
2339 2033 675 496 411 236 172 108
78 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2600 2621 3011 3899 1163
Size: 573 864 21 390 888 3889
Fragments arranged by size:
3889 888 864 573 390 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2175 4686 4771 5842 2064
Size: 111 2511 85 1071 2847
Fragments arranged by size:
2847 2511 1071 111 85
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2153 2175 2668 2864 2917 3063
Size: 399 274 89 22 493 196 53 146
Cuts at: 3063 3414 3423 3520 3723 3944 4686 4771 5842
Size: 351 9 97 203 221 742 85 1071
Cuts at: 5842 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 493 399 351 274 221
203 196 146 97 89 85 53 22
9
BspEI T'CCGG_A
Cuts at: 2241 3282 6288 2241
Size: 1041 3006 2578
Fragments arranged by size:
3006 2578 1041
BspHI T'CATG_A
Cuts at: 3162 4514 6244 3162
Size: 1352 1730 3543
Fragments arranged by size:
3543 1730 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 2983 3537 3913 3980 844
Size: 1455 684 554 376 67 3489
Fragments arranged by size:
3489 1455 684 554 376 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2472 4512 1394
Size: 429 649 2040 3507
Fragments arranged by size:
3507 2040 649 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3092 5120 5294 65
Size: 1183 1844 2028 174 1396
Fragments arranged by size:
2028 1844 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2351 4685 5697 2351
Size: 2334 1012 3279
Fragments arranged by size:
3279 2334 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2725 794
Size: 1931 4694
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2906 2623
Size: 283 6342
BstYI r'GATC_y
Cuts at: 1972 2334 3442 3451 3574 3885 3955 4322 4707
Size: 362 1108 9 123 311 70 367 385
Cuts at: 4707 4724 6176 6262 6274 6620 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1108 385 367 362 346 311
123 86 70 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2198 2776 3348 2198
Size: 578 572 5475
Fragments arranged by size:
5475 578 572
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2368 2564 2889 3663 4141 4935 4955 2368
Size: 196 325 774 478 794 20 4038
Fragments arranged by size:
4038 794 774 478 325 196 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2886 4072
Size: 54 1425 50 275 18 564 492 1186
Cuts at: 4072 4961 6590 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1186 889 564 492 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3072 3284 3806 4555 1353
Size: 1719 212 522 749 3423
Fragments arranged by size:
3423 1719 749 522 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3194 5212 5586 5732 387
Size: 1064 138 1605 2018 374 146 1280
Fragments arranged by size:
2018 1605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 2173
Size: 6625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2759 3050 3716 3787 4688 5285
Size: 393 675 452 291 666 71 901 597
Cuts at: 5285 6072 6340 1239
Size: 787 268 1524
Fragments arranged by size:
1524 901 787 675 666 597 452 393
291 268 71
EcoO109I rG'GnC_Cy
Cuts at: 2913 3025 3748 3826 2913
Size: 112 723 78 5712
Fragments arranged by size:
5712 723 112 78
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
EcoRV GAT'ATC
Cuts at: 2467 2467
Size: 6625
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 5772 1678
Size: 4094 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2456 2488 2759
Size: 32 135 32 736 32 116 32 271
Cuts at: 2759 2791 3135 3167 5266 5298 5340 5372 1373
Size: 32 344 32 2099 32 42 32 2626
Fragments arranged by size:
2626 2099 736 344 271 135 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 3969 4215 669
Size: 988 281 565 1466 246 3079
Fragments arranged by size:
3079 1466 988 565 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 2222 3950 2222
Size: 1728 4897
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2834 5739 5923 1305
Size: 1529 2905 184 2007
Fragments arranged by size:
2905 2007 1529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2888 10
Size: 54 1425 343 564 492 3747
Fragments arranged by size:
3747 1425 564 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2621 2867 4583 4942 5101 518
Size: 648 1293 162 246 1716 359 159 2042
Fragments arranged by size:
2042 1716 1293 648 359 246 162 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2480 2605 2902 3256 3607 3736 3742
Size: 621 253 125 297 354 351 129 6
Cuts at: 3742 4041 4718 5866 6111 1606
Size: 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 354 351 299 297
253 245 129 125 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2846 2930 3124 3983 5528 1132
Size: 97 1617 84 194 859 1545 2229
Fragments arranged by size:
2229 1617 1545 859 194 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 3120 5524 3120
Size: 2404 4221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2820 1108
Size: 1712 4913
PfoI T'CCnGG_A
Cuts at: 3312 3438 3456 3312
Size: 126 18 6481
Fragments arranged by size:
6481 126 18
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2026 2058 4694 4726 6207 6239 2026
Size: 32 2636 32 1481 32 2412
Fragments arranged by size:
2636 2412 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3025
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PspOMI G'GGCC_C
Cuts at: 2913 2913
Size: 6625
PspXI vC'TCGA_Gb
Cuts at: 3138 3138
Size: 6625
PstI C_TGCA'G
Cuts at: 830 2194 2310 2479 2730 2797 3114 3252 3741
Size: 1364 116 169 251 67 317 138 489
Cuts at: 3741 3747 3977 830
Size: 6 230 3478
Fragments arranged by size:
3478 1364 489 317 251 230 169 138
116 67 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2227 2480 2902 3607 3736 3742 2227
Size: 253 422 705 129 6 5110
Fragments arranged by size:
5110 705 422 253 129 6
SacI G_AGCT'C
Cuts at: 2175 2175
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 3806 3806
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 2310 3977 2310
Size: 1667 4958
ScaI AGT'ACT
Cuts at: 2451 4873 6585 2451
Size: 2422 1712 2491
Fragments arranged by size:
2491 2422 1712
SfcI C'TryA_G
Cuts at: 826 1045 2190 2306 2475 2726 2793 3110 3248
Size: 219 1145 116 169 251 67 317 138
Cuts at: 3248 3737 3743 3973 5108 5789 5980 826
Size: 489 6 230 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 681 489 317 251 230
219 191 169 138 116 67 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2393 2393
Size: 6625
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3138 4729 5630 5892 820
Size: 197 926 1195 1591 901 262 1553
Fragments arranged by size:
1591 1553 1195 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 2732 3428 1517
Size: 1215 696 4714
Fragments arranged by size:
4714 1215 696
StyI C'CwwG_G
Cuts at: 513 1065 2004 4064 513
Size: 552 939 2060 3074
Fragments arranged by size:
3074 2060 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2879 3710 3809 4652 4669 4822 5007
Size: 1255 1299 831 99 843 17 153 185
Cuts at: 5007 5452 325
Size: 445 1498
Fragments arranged by size:
1498 1299 1255 843 831 445 185 153
99 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2449 3601 4871
Size: 274 80 33 51 1443 472 1152 1270
Cuts at: 4871 6583 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1270 1152 472 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6585 6617 6585
Size: 32 6593
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2817 2817
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 3138 1943
Size: 1195 5430
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 4754 4754
Size: 6625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI