Restriction map for
pFN21AE6784
(Circular) (Six-base) MAPSORT of: pFN21AE6784.seq Check: 4945 from: 1 to: 6589
pFN21AE6784 6589 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 3687 3932 1937
Size: 703 1047 245 4594
Fragments arranged by size:
4594 1047 703 245
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3124 3509 4652 6036 6304 1632
Size: 1492 385 1143 1384 268 1917
Fragments arranged by size:
1917 1492 1384 1143 385 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 2611 5488 6465 1203
Size: 1408 2877 977 1327
Fragments arranged by size:
2877 1408 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AlwNI CAG_nnn'CTG
Cuts at: 2408 4332 5904 2408
Size: 1924 1572 3093
Fragments arranged by size:
3093 1924 1572
ApaI G_GGCC'C
Cuts at: 2231 2517 2231
Size: 286 6303
ApaLI G'TGCA_C
Cuts at: 2595 4646 5802 2595
Size: 2051 1156 3382
Fragments arranged by size:
3382 2051 1156
ApoI r'AATT_y
Cuts at: 1493 3898 4124 4276 6385 1493
Size: 2405 226 152 2109 1697
Fragments arranged by size:
2405 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3048 5143 160
Size: 872 2016 2095 1606
Fragments arranged by size:
2095 2016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 2343 2500 3785 3914 1870
Size: 73 140 260 157 1285 129 4545
Fragments arranged by size:
4545 1285 260 157 140 129 73
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2326 3115 3910 5365
Size: 325 217 627 248 291 789 795 1455
Cuts at: 5365 618
Size: 1842
Fragments arranged by size:
1842 1455 795 789 627 325 291 248
217
BanII G_rGCy'C
Cuts at: 1391 2133 2231 2517 3908 1391
Size: 742 98 286 1391 4072
Fragments arranged by size:
4072 1391 742 286 98
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5618
BbvCI CC'TCA_GC
Cuts at: 2135 2135
Size: 6589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2288 4481 5691 2288
Size: 2193 1210 3186
Fragments arranged by size:
3186 2193 1210
BclI T'GATC_A
Cuts at: 1548 3061 1548
Size: 1513 5076
BfrBI ATG'CAT
Cuts at: 3750 3750
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5200 136
Size: 107 122 71 631 605 3528 1525
Fragments arranged by size:
3528 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2231 2329 2517 2599 3120 3335 4650 5806
Size: 441 98 188 82 521 215 1315 1156
Cuts at: 5806 1790
Size: 2573
Fragments arranged by size:
2573 1315 1156 521 441 215 188 98
82
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4320 5278 442
Size: 416 53 83 399 24 2903 958 1753
Fragments arranged by size:
2903 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 5249 1239
Size: 1490 2520 2579
Fragments arranged by size:
2579 2520 1490
Bpu10I CC'TnA_GC
Cuts at: 2135 2730 3175 2135
Size: 595 445 5549
Fragments arranged by size:
5549 595 445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2286 2898 4714 5579 5877 2286
Size: 612 1816 865 298 2998
Fragments arranged by size:
2998 1816 865 612 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5252 882
Size: 617 3753 2219
Fragments arranged by size:
3753 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2614 4380 493
Size: 711 1410 1766 2702
Fragments arranged by size:
2702 1766 1410 711
BsaBI GATnn'nnATC
Cuts at: 2778 6583 2778
Size: 3805 2784
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 4396 4778 5413
Size: 53 83 186 936 503 2360 382 635
Cuts at: 5413 275
Size: 1451
Fragments arranged by size:
2360 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3185 5022 5420 5694 5841 6252 3185
Size: 1837 398 274 147 411 3522
Fragments arranged by size:
3522 1837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6364
Fragments arranged by size:
6364 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 1186
Size: 906 5683
BseYI C'CCAG_C
Cuts at: 2046 2121 5792 6288 2046
Size: 75 3671 496 2347
Fragments arranged by size:
3671 2347 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2266 3617 1163
Size: 573 530 1351 4135
Fragments arranged by size:
4135 1351 573 530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2256 4039 4800 5828 1540
Size: 275 186 255 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 2460 2599 3597 4650 4735 5806 2460
Size: 139 998 1053 85 1071 3243
Fragments arranged by size:
3243 1071 1053 998 139 85
BsmI GAATG_Cn'
Cuts at: 4100 4193 4100
Size: 93 6496
BsmBI CGTCTCn'nnnn_
Cuts at: 3782 3782
Size: 6589
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2231 2329 2460 2517 2599 3120
Size: 399 343 98 98 131 57 82 521
Cuts at: 3120 3335 3597 3908 4650 4735 5806 1391
Size: 215 262 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 521 399 343 311 262
215 131 98 98 85 82 57
BspEI T'CCGG_A
Cuts at: 6252 6252
Size: 6589
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 3944 844
Size: 1444 1656 3489
Fragments arranged by size:
3489 1656 1444
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 4476 1394
Size: 429 265 2388 3507
Fragments arranged by size:
3507 2388 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 3750 3846 5084 5258 65
Size: 1183 2502 96 1238 174 1396
Fragments arranged by size:
2502 1396 1238 1183 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2285 5233 5420 1700
Size: 110 445 30 2948 187 2869
Fragments arranged by size:
2948 2869 445 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 3386 4649 5661 3386
Size: 1263 1012 4314
Fragments arranged by size:
4314 1263 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6589
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584
Size: 1947 367 385 17 1452 86 12 346
Cuts at: 6584 1972
Size: 1977
Fragments arranged by size:
1977 1947 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2590 2590
Size: 6589
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2199 2391 513
Size: 552 939 66 129 192 4711
Fragments arranged by size:
4711 939 552 192 129 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3017 487
Size: 683 56 1791 4059
Fragments arranged by size:
4059 1791 683 56
BtsI GCAGTG_nn'
Cuts at: 4105 4899 4919 4105
Size: 794 20 5775
Fragments arranged by size:
5775 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3373 3893 3373
Size: 520 6069
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2253 2530
Size: 54 1425 50 275 18 282 141 277
Cuts at: 2530 2632 2872 4036 4925 6554 8
Size: 102 240 1164 889 1629 43
Fragments arranged by size:
1629 1425 1164 889 282 277 275 240
141 102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2253 4036 1537
Size: 275 441 1783 4090
Fragments arranged by size:
4090 1783 441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3501 4519 1353
Size: 2148 1018 3423
Fragments arranged by size:
3423 2148 1018
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5176 5550 5696 387
Size: 1064 138 3587 374 146 1280
Fragments arranged by size:
3587 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2729 3124 3509 4652 5249 6036 6304
Size: 393 1097 395 385 1143 597 787 268
Cuts at: 6304 1239
Size: 1524
Fragments arranged by size:
1524 1143 1097 787 597 395 393 385
268
EcoO109I rG'GnC_Cy
Cuts at: 2227 2513 2514 2227
Size: 286 1 6302
Fragments arranged by size:
6302 286 1
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3530 3530
Size: 6589
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2039 5736 1678
Size: 361 3697 2531
Fragments arranged by size:
3697 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2084 2116 3046
Size: 32 135 32 479 32 1 32 930
Cuts at: 3046 3078 3584 3616 3680 3712 5230 5262 5304
Size: 32 506 32 64 32 1518 32 42
Cuts at: 5304 5336 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 930 506 479 135 64 42
32 32 32 32 32 32 32 32
32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 2655 2695 3933 4179 669
Size: 988 281 229 488 40 1238 246 3079
Fragments arranged by size:
3079 1238 988 488 281 246 229 40
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 3282 3306 3852 4188 3282
Size: 24 546 336 5683
Fragments arranged by size:
5683 546 336 24
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5703 5887 1305
Size: 4398 184 2007
Fragments arranged by size:
4398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2532 2634 2874 10
Size: 54 1425 343 700 102 240 3725
Fragments arranged by size:
3725 1425 700 343 240 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2616 4547 4906 5065 518
Size: 648 1450 1931 359 159 2042
Fragments arranged by size:
2042 1931 1450 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2201 4005 4682 5830 6075 1606
Size: 595 1804 677 1148 245 2120
Fragments arranged by size:
2120 1804 1148 677 595 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 6589
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 2391 513
Size: 552 939 387 4711
Fragments arranged by size:
4711 939 552 387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NsiI A_TGCA'T
Cuts at: 3752 3752
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3002 3947 5492 1132
Size: 97 1773 945 1545 2229
Fragments arranged by size:
2229 1773 1545 945 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6589
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PfoI T'CCnGG_A
Cuts at: 3517 3517
Size: 6589
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 3621 4159 3621
Size: 538 6051
PspOMI G'GGCC_C
Cuts at: 2227 2513 2227
Size: 286 6303
PstI C_TGCA'G
Cuts at: 830 3941 830
Size: 3111 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
SacII CC_GC'GG
Cuts at: 2202 2202
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 2052 2750 2052
Size: 698 5891
SfcI C'TryA_G
Cuts at: 826 1045 3937 5072 5753 5944 826
Size: 219 2892 1135 681 191 1471
Fragments arranged by size:
2892 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 2285 1700
Size: 585 6004
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2301 2913 3785 4693 5594 5856
Size: 197 926 358 612 872 908 901 262
Cuts at: 5856 820
Size: 1553
Fragments arranged by size:
1553 926 908 901 872 612 358 262
197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 3196 4513 5
Size: 47 3144 1317 2081
Fragments arranged by size:
3144 2081 1317 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 3221 3681 4028 513
Size: 552 939 387 830 460 347 3074
Fragments arranged by size:
3074 939 830 552 460 387 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 4633 4786 4971 5416 325
Size: 1255 3036 17 153 185 445 1498
Fragments arranged by size:
3036 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3318 4835 6547
Size: 274 80 33 51 1443 1341 1517 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
1712 1517 1443 1341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6549 6581 6549
Size: 32 6557
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2642 2642
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 3785 1943
Size: 1842 4747
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 2849 3430 3510 4718 2849
Size: 581 80 1208 4720
Fragments arranged by size:
4720 1208 581 80
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SfiI SrfI SwaI