Restriction map for
pFN21AE6780
(Circular) (Six-base) MAPSORT of: pFN21AE6780.seq Check: 5761 from: 1 to: 6406
pFN21AE6780 6406 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2406 3749 1937
Size: 469 1343 4594
Fragments arranged by size:
4594 1343 469
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4469 5853 6121 1632
Size: 2837 1384 268 1917
Fragments arranged by size:
2837 1917 1384 268
AfeI AGC'GCT
Cuts at: 3635 3635
Size: 6406
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2366 1166
Size: 1200 5206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 3677 1184
Size: 1554 939 3913
Fragments arranged by size:
3913 1554 939
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2468 2500 2315
Size: 32 121 32 6221
Fragments arranged by size:
6221 121 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2473 3043 4149 5721 2473
Size: 570 1106 1572 3158
Fragments arranged by size:
3158 1572 1106 570
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2024 3715 3941 4093 6202 1493
Size: 531 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 531 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 3731 1870
Size: 73 407 1381 4545
Fragments arranged by size:
4545 1381 407 73
AvrII C'CTAG_G
Cuts at: 3388 3388
Size: 6406
BamHI G'GATC_C
Cuts at: 2346 2493 3736 4103 2346
Size: 147 1243 367 4649
Fragments arranged by size:
4649 1243 367 147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2841 3354 3727 5182
Size: 325 217 627 660 394 513 373 1455
Cuts at: 5182 618
Size: 1842
Fragments arranged by size:
1842 1455 660 627 513 394 373 325
217
BanII G_rGCy'C
Cuts at: 1391 2141 3725 1391
Size: 750 1584 4072
Fragments arranged by size:
4072 1584 750
BbeI G_GCGC'C
Cuts at: 2451 2451
Size: 6406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5435
BbvCI CC'TCA_GC
Cuts at: 2517 2702 3414 2517
Size: 185 712 5509
Fragments arranged by size:
5509 712 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 2238 1548
Size: 690 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2624 2712 5017
Size: 107 122 71 631 605 952 88 2305
Cuts at: 5017 136
Size: 1525
Fragments arranged by size:
2305 1525 952 631 605 122 107 88
71
BglII A'GATC_T
Cuts at: 3280 6401 3280
Size: 3121 3285
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2844 2867 2904 4467 5623 1790
Size: 1054 23 37 1563 1156 2573
Fragments arranged by size:
2573 1563 1156 1054 37 23
BmgBI CAC'GTC
Cuts at: 1318 2200 3019 1318
Size: 882 819 4705
Fragments arranged by size:
4705 882 819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 4137 5095
Size: 416 53 83 399 24 883 1837 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1837 1753 958 883 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2495 3534 3583 5066 1239
Size: 1256 1039 49 1483 2579
Fragments arranged by size:
2579 1483 1256 1039 49
Bpu10I CC'TnA_GC
Cuts at: 2517 2702 3414 2517
Size: 185 712 5509
Fragments arranged by size:
5509 712 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2444 3595 4531 5396 5694 2054
Size: 390 1151 936 865 298 2766
Fragments arranged by size:
2766 1151 936 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2804 2888 3382 5069 882
Size: 617 1305 84 494 1687 2219
Fragments arranged by size:
2219 1687 1305 617 494 84
BsaAI yAC'GTr
Cuts at: 493 1204 2398 4197 493
Size: 711 1194 1799 2702
Fragments arranged by size:
2702 1799 1194 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 3436 4213 4595
Size: 53 83 186 936 915 988 777 382
Cuts at: 4595 5230 275
Size: 635 1451
Fragments arranged by size:
1451 988 936 915 777 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2468 2492 2498
Size: 30 165 30 912 30 123 24 6
Cuts at: 2498 2522 2547 2577 3362 3392 1178
Size: 24 25 30 785 30 4192
Fragments arranged by size:
4192 912 785 165 123 30 30 30
30 30 25 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 5049
BseYI C'CCAG_C
Cuts at: 3630 5609 6105 3630
Size: 1979 496 3931
Fragments arranged by size:
3931 1979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 3119 3257 1163
Size: 573 486 897 138 4312
Fragments arranged by size:
4312 897 573 486 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2184 2312 3423 4467 4552 5623 2070
Size: 114 128 1111 1044 85 1071 2853
Fragments arranged by size:
2853 1111 1071 1044 128 114 85
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 3443 3614 3443
Size: 171 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2141 2184 2312 2844 2867 2904
Size: 399 280 71 43 128 532 23 37
Cuts at: 2904 3423 3725 4467 4552 5623 1391
Size: 519 302 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 519 399 302 280
128 85 71 43 37 23
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3282 3761 844
Size: 2438 479 3489
Fragments arranged by size:
3489 2438 479
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3669 4901 5075 65
Size: 1183 2421 1232 174 1396
Fragments arranged by size:
2421 1396 1232 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2391 5050 5237 1700
Size: 110 581 2659 187 2869
Fragments arranged by size:
2869 2659 581 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2813 794
Size: 2019 4387
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 3007 3505 3638 3007
Size: 498 133 5775
Fragments arranged by size:
5775 498 133
BstYI r'GATC_y
Cuts at: 1972 2346 2470 2493 3280 3453 3468 3736 4103
Size: 374 124 23 787 173 15 268 367
Cuts at: 4103 4488 4505 5957 6043 6055 6401 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 787 385 374 367 346 268
173 124 86 23 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2466 2466
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 2902 2957 3922 4716 4736 2547
Size: 355 55 965 794 20 4217
Fragments arranged by size:
4217 965 794 355 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 529
Size: 35 2650 35 3686
Fragments arranged by size:
3686 2650 35 35
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2788 2997
Size: 54 1425 50 275 18 186 772 209
Cuts at: 2997 3327 3853 4742 6371 8
Size: 330 526 889 1629 43
Fragments arranged by size:
1629 1425 889 772 526 330 275 209
186 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 3290 3365 4336 1353
Size: 753 1184 75 971 3423
Fragments arranged by size:
3423 1184 971 753 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 3534 3583 4469 5066 5853 6121
Size: 393 863 1039 49 886 597 787 268
Cuts at: 6121 1239
Size: 1524
Fragments arranged by size:
1524 1039 886 863 787 597 393 268
49
EcoNI CCTnn'n_nnAGG
Cuts at: 2660 3413 2660
Size: 753 5653
EcoO109I rG'GnC_Cy
Cuts at: 2719 3255 2719
Size: 536 5870
EcoRI G'AATT_C
Cuts at: 2024 3715 4093 2024
Size: 1691 378 4337
Fragments arranged by size:
4337 1691 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2635 2437
Size: 198 6208
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2451 3513 3637 5553 1678
Size: 773 1062 124 1916 2531
Fragments arranged by size:
2531 1916 1062 773 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2492 2524 2651
Size: 32 135 32 658 32 230 32 127
Cuts at: 2651 2683 5047 5079 5121 5153 1373
Size: 32 2364 32 42 32 2626
Fragments arranged by size:
2626 2364 658 230 135 127 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 2447 2447
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 2790 2999 3329 10
Size: 54 1425 343 186 772 209 330 3087
Fragments arranged by size:
3087 1425 772 343 330 209 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2366 4364 4723 4882 518
Size: 648 1074 126 1998 359 159 2042
Fragments arranged by size:
2042 1998 1074 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 3115 3822 4499 5647 5892 1606
Size: 1509 707 677 1148 245 2120
Fragments arranged by size:
2120 1509 1148 707 677 245
NaeI GCC'GGC
Cuts at: 2393 2393
Size: 6406
NarI GG'CG_CC
Cuts at: 2448 2448
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NgoMIV G'CCGG_C
Cuts at: 2391 2391
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 2816 3478 3764 5309 1132
Size: 97 1587 662 286 1545 2229
Fragments arranged by size:
2229 1587 1545 662 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6406
PfoI T'CCnGG_A
Cuts at: 2028 2496 3126 3180 2028
Size: 468 630 54 5254
Fragments arranged by size:
5254 630 468 54
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PmlI CAC'GTG
Cuts at: 2398 2398
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 2468 2500 4475 4507 5988 6020 2315
Size: 32 121 32 1975 32 1481 32 2701
Fragments arranged by size:
2701 1975 1481 121 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3255 3255
Size: 6406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2055 2554 2952 3114 3758 830
Size: 1225 499 398 162 644 3478
Fragments arranged by size:
3478 1225 644 499 398 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 3115 3115
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 3255 3255
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SexAI A'CCwGG_T
Cuts at: 2061 2991 2061
Size: 930 5476
SfcI C'TryA_G
Cuts at: 826 1045 2051 2372 2550 2948 3110 3531 3700
Size: 219 1006 321 178 398 162 421 169
Cuts at: 3700 3754 4889 5570 5761 826
Size: 54 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 421 398 321 219
191 178 169 162 54
SfoI GGC'GCC
Cuts at: 2449 2449
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 2352 3733 1872
Size: 480 1381 4545
Fragments arranged by size:
4545 1381 480
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2459 3610 4510 5411 5673
Size: 197 926 126 390 1151 900 901 262
Cuts at: 5673 820
Size: 1553
Fragments arranged by size:
1553 1151 926 901 900 390 262 197
126
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 2816 3764 1132
Size: 1684 948 3774
Fragments arranged by size:
3774 1684 948
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2588 3060 3161 3698 1517
Size: 1071 472 101 537 4225
Fragments arranged by size:
4225 1071 537 472 101
StyI C'CwwG_G
Cuts at: 513 1065 2004 2619 3000 3388 3693 3845 513
Size: 552 939 615 381 388 305 152 3074
Fragments arranged by size:
3074 939 615 552 388 381 305 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4433 4450 4603 4788 5233 325
Size: 1255 2853 17 153 185 445 1498
Fragments arranged by size:
2853 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 3027 4652
Size: 274 80 33 51 1443 138 912 1625
Cuts at: 4652 6364 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 912 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2547 2579 6366 6398 2547
Size: 32 3787 32 2555
Fragments arranged by size:
3787 2555 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2350 3731 1870
Size: 480 1381 4545
Fragments arranged by size:
4545 1381 480
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SapI SfiI SrfI
SwaI