Restriction map for
pFN21AE6778
(Circular) (Six-base) MAPSORT of: pFN21AE6778.seq Check: 1067 from: 1 to: 6325
pFN21AE6778 6325 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2340 3193 2340
Size: 853 5472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3668 1937
Size: 1731 4594
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2555 3318 4388 5772 6040 1632
Size: 455 468 763 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 763 468 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 2550 5156 2550
Size: 2606 3719
AhdI GACnn_n'nnGTC
Cuts at: 3331 5054 3331
Size: 1723 4602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3467 1184
Size: 2283 4042
AlwNI CAG_nnn'CTG
Cuts at: 4068 5640 4068
Size: 1572 4753
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 3634 3860 4012 6121 1493
Size: 2141 226 152 2109 1697
Fragments arranged by size:
2141 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
BamHI G'GATC_C
Cuts at: 2657 3655 4022 2657
Size: 998 367 4960
Fragments arranged by size:
4960 998 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3646 5101 618
Size: 325 217 627 1859 1455 1842
Fragments arranged by size:
1859 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2686 2785 2911 3021 3165 3623 3644 1391
Size: 1295 99 126 110 144 458 21 4072
Fragments arranged by size:
4072 1295 458 144 126 110 99 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 2820 3138 928
Size: 971 585 336 318 4115
Fragments arranged by size:
4115 971 585 336 318
BbvCI CC'TCA_GC
Cuts at: 3423 3423
Size: 6325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 3409 1548
Size: 1861 4464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2671 6320 2671
Size: 3649 2676
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 2398 4386 5542 1790
Size: 608 1988 1156 2573
Fragments arranged by size:
2573 1988 1156 608
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4056 5014 442
Size: 416 53 83 399 24 2639 958 1753
Fragments arranged by size:
2639 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2663 3637 2341
Size: 322 974 5029
Fragments arranged by size:
5029 974 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2324 2504 2895 4985 1239
Size: 969 116 180 391 2090 2579
Fragments arranged by size:
2579 2090 969 391 180 116
Bpu10I CC'TnA_GC
Cuts at: 3387 3423 3387
Size: 36 6289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4450 5315 5613 4450
Size: 865 298 5162
Fragments arranged by size:
5162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2999 3334 3563 4988 882
Size: 617 1500 335 229 1425 2219
Fragments arranged by size:
2219 1500 1425 617 335 229
BsaAI yAC'GTr
Cuts at: 493 1204 3040 3067 4116 493
Size: 711 1836 27 1049 2702
Fragments arranged by size:
2702 1836 1049 711 27
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2550 2660 4758 5156 5430 5577 5988 2550
Size: 110 2098 398 274 147 411 2887
Fragments arranged by size:
2887 2098 411 398 274 147 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2206 2236 3331 3361 3411
Size: 30 165 30 803 30 1095 30 50
Cuts at: 3411 3441 1178
Size: 30 4062
Fragments arranged by size:
4062 1095 803 165 50 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 2270 2321 2480 3079 3332 1186
Size: 1025 59 51 159 599 253 4179
Fragments arranged by size:
4179 1025 599 253 159 59 51
BseYI C'CCAG_C
Cuts at: 2523 5528 6024 2523
Size: 3005 496 2824
Fragments arranged by size:
3005 2824 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3300 3387 1163
Size: 573 1564 87 4101
Fragments arranged by size:
4101 1564 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2520 3775 4536 5564 1540
Size: 275 186 519 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 519 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2686 2785 3021 3623 4386 4471 5542 2325
Size: 361 99 236 602 763 85 1071 3108
Fragments arranged by size:
3108 1071 763 602 361 236 99 85
BsmI GAATG_Cn'
Cuts at: 3534 3836 3929 3534
Size: 302 93 5930
Fragments arranged by size:
5930 302 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2398 2686 2785 2911 3021 3165
Size: 399 535 73 288 99 126 110 144
Cuts at: 3165 3623 3644 4386 4471 5542 1391
Size: 458 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 535 458 399 288 144
126 110 99 85 73 21
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 3193 3680 844
Size: 1496 853 487 3489
Fragments arranged by size:
3489 1496 853 487
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2476 3034 3322 4212 1394
Size: 429 452 201 558 288 890 3507
Fragments arranged by size:
3507 890 558 452 429 288 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 4820 4994 65
Size: 1183 3572 174 1396
Fragments arranged by size:
3572 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 2550 4969 5156 1700
Size: 110 695 45 2419 187 2869
Fragments arranged by size:
2869 2419 695 187 110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstXI CCAn_nnnn'nTGG
Cuts at: 2636 3067 3478 2636
Size: 431 411 5483
Fragments arranged by size:
5483 431 411
BstYI r'GATC_y
Cuts at: 1972 2657 2671 3655 4022 4407 4424 5876 5962
Size: 685 14 984 367 385 17 1452 86
Cuts at: 5962 5974 6320 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 984 685 385 367 346 86
17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2635 3351 3525 513
Size: 552 1570 716 174 3313
Fragments arranged by size:
3313 1570 716 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 2447 3841 4635 4655 2447
Size: 1394 794 20 4117
Fragments arranged by size:
4117 1394 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DraIII CAC_nnn'GTG
Cuts at: 3365 3365
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 3151 3772
Size: 54 1425 50 275 18 453 868 621
Cuts at: 3772 4661 6290 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 868 621 453 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3294 3324 3611 4255 1353
Size: 1941 30 287 644 3423
Fragments arranged by size:
3423 1941 644 287 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2684 2783 3019 3621 2684
Size: 99 236 602 5388
Fragments arranged by size:
5388 602 236 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2208 2324 2504 2555 2895 3318
Size: 393 455 121 116 180 51 340 423
Cuts at: 3318 4388 4985 5772 6040 1239
Size: 1070 597 787 268 1524
Fragments arranged by size:
1524 1070 787 597 455 423 393 340
268 180 121 116 51
EcoO109I rG'GnC_Cy
Cuts at: 2479 3293 3464 2479
Size: 814 171 5340
Fragments arranged by size:
5340 814 171
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
EcoRV GAT'ATC
Cuts at: 2134 2134
Size: 6325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2066
Size: 6325
FspI TGC'GCA
Cuts at: 2453 4831 2453
Size: 2378 3947
HaeII r_GCGC'y
Cuts at: 1678 5472 1678
Size: 3794 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 3069 3101 3317
Size: 32 135 32 784 32 681 32 216
Cuts at: 3317 3349 3362 3394 4966 4998 5040 5072 1373
Size: 32 13 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 784 681 216 135 42 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2030 3669 3915 669
Size: 988 281 92 1639 246 3079
Fragments arranged by size:
3079 1639 988 281 246 92
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3237 3924 3237
Size: 687 5638
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5439 5623 1305
Size: 4134 184 2007
Fragments arranged by size:
4134 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4283 4642 4801 518
Size: 648 3117 359 159 2042
Fragments arranged by size:
3117 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2302 2512 3557 3741 4418 5566 5811
Size: 501 195 210 1045 184 677 1148 245
Cuts at: 5811 1606
Size: 2120
Fragments arranged by size:
2120 1148 1045 677 501 245 210 195
184
NaeI GCC'GGC
Cuts at: 2507 2507
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 2635 3351 513
Size: 552 1570 716 3487
Fragments arranged by size:
3487 1570 716 552
NdeI CA'TA_TG
Cuts at: 387 3544 387
Size: 3157 3168
NgoMIV G'CCGG_C
Cuts at: 2505 2505
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 3683 5228 1132
Size: 97 2454 1545 2229
Fragments arranged by size:
2454 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PfoI T'CCnGG_A
Cuts at: 2481 3335 2481
Size: 854 5471
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PmlI CAC'GTG
Cuts at: 3040 3040
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2479
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 2430 2759 3895 2430
Size: 329 1136 4860
Fragments arranged by size:
4860 1136 329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3366 3398 3366
Size: 32 6293
PstI C_TGCA'G
Cuts at: 830 2202 2337 2358 2691 3216 3677 830
Size: 1372 135 21 333 525 461 3478
Fragments arranged by size:
3478 1372 525 461 333 135 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2107 2302 2512 2107
Size: 195 210 5920
Fragments arranged by size:
5920 210 195
SacI G_AGCT'C
Cuts at: 2686 2785 3021 3623 2686
Size: 99 236 602 5388
Fragments arranged by size:
5388 602 236 99
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SapI GCTCTTCn'nnn_
Cuts at: 3611 3611
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 2337 3677 2337
Size: 1340 4985
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2198 2333 2354 2687 3212 3252 3673
Size: 219 1153 135 21 333 525 40 421
Cuts at: 3673 4808 5489 5680 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 681 525 421 333 219
191 135 40 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4429 5330 5592 820
Size: 197 926 2486 901 262 1553
Fragments arranged by size:
2486 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 2635 2741 3351 3471 3764 513
Size: 552 1570 106 610 120 293 3074
Fragments arranged by size:
3074 1570 610 552 293 120 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 3193 4352 4369 4522 4707 5152
Size: 1255 1062 551 1159 17 153 185 445
Cuts at: 5152 325
Size: 1498
Fragments arranged by size:
1498 1255 1159 1062 551 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4571 6283 96
Size: 274 80 33 51 1443 2594 1712 138
Fragments arranged by size:
2594 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 3331 3363 3411 3443 6285 6317 2206
Size: 32 1093 32 48 32 2842 32 2214
Fragments arranged by size:
2842 2214 1093 48 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3589 1316
Size: 2273 4052
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2782 2782
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI