Restriction map for
pFN21AE6771
(Circular) (Six-base) MAPSORT of: pFN21AE6771.seq Check: 5066 from: 1 to: 6394
pFN21AE6771 6394 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3737 1937
Size: 1800 4594
Acc65I G'GTAC_C
Cuts at: 2714 3096 3715 2714
Size: 382 619 5393
Fragments arranged by size:
5393 619 382
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2946 3270 3670 4457 5841 6109 1632
Size: 1314 324 400 787 1384 268 1917
Fragments arranged by size:
1917 1384 1314 787 400 324 268
AflII C'TTAA_G
Cuts at: 820 1017 2978 820
Size: 197 1961 4236
Fragments arranged by size:
4236 1961 197
AflIII A'CryG_T
Cuts at: 1203 5293 6270 1203
Size: 4090 977 1327
Fragments arranged by size:
4090 1327 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 2284 5123 2284
Size: 2839 3555
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6394
AlwNI CAG_nnn'CTG
Cuts at: 2450 4137 5709 2450
Size: 1687 1572 3135
Fragments arranged by size:
3135 1687 1572
ApaI G_GGCC'C
Cuts at: 2473 2473
Size: 6394
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 3703 3929 4081 6190 1493
Size: 2210 226 152 2109 1697
Fragments arranged by size:
2210 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 3477 3719 1870
Size: 73 274 1260 242 4545
Fragments arranged by size:
4545 1260 274 242 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3664 3697 3664
Size: 33 6361
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2344 2432 2714 3096
Size: 325 217 627 312 245 88 282 382
Cuts at: 3096 3269 3715 5170 618
Size: 173 446 1455 1842
Fragments arranged by size:
1842 1455 627 446 382 325 312 282
245 217 173 88
BanII G_rGCy'C
Cuts at: 1391 2120 2473 2648 3335 3478 3528 3713 1391
Size: 729 353 175 687 143 50 185 4072
Fragments arranged by size:
4072 729 687 353 185 175 143 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 3674 928
Size: 971 702 1073 3648
Fragments arranged by size:
3648 1073 971 702
BbvCI CC'TCA_GC
Cuts at: 2775 2775
Size: 6394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4585 4619 1903
Size: 34 2648 34 3678
Fragments arranged by size:
3678 2648 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4286 5496 4286
Size: 1210 5184
BclI T'GATC_A
Cuts at: 1548 2842 1548
Size: 1294 5100
BfrBI ATG'CAT
Cuts at: 2397 2397
Size: 6394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3290 5005
Size: 107 122 71 631 605 337 1281 1715
Cuts at: 5005 136
Size: 1525
Fragments arranged by size:
1715 1525 1281 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6389 6389
Size: 6394
BlpI GC'TnA_GC
Cuts at: 3811 3811
Size: 6394
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2102 2473 3301 4455 5611 1790
Size: 254 58 371 828 1154 1156 2573
Fragments arranged by size:
2573 1156 1154 828 371 254 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 2438 2766
Size: 416 53 83 399 24 885 136 328
Cuts at: 2766 2839 3095 4125 5083 442
Size: 73 256 1030 958 1753
Fragments arranged by size:
1753 1030 958 885 416 399 328 256
136 83 73 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2835 3481 5054 1239
Size: 1596 646 1573 2579
Fragments arranged by size:
2579 1596 1573 646
Bpu10I CC'TnA_GC
Cuts at: 2775 2775
Size: 6394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4519 5384 5682 4519
Size: 865 298 5231
Fragments arranged by size:
5231 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 2562 3635 5057 882
Size: 617 793 270 1073 1422 2219
Fragments arranged by size:
2219 1422 1073 793 617 270
BsaAI yAC'GTr
Cuts at: 493 1204 4185 493
Size: 711 2981 2702
Fragments arranged by size:
2981 2702 711
BsaBI GATnn'nnATC
Cuts at: 2844 6388 2844
Size: 3544 2850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 4583 5218 275
Size: 53 83 186 936 2668 382 635 1451
Fragments arranged by size:
2668 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4827 5225 5499 5646 6057 4827
Size: 398 274 147 411 5164
Fragments arranged by size:
5164 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2655 2685 1178
Size: 30 165 30 1252 30 4887
Fragments arranged by size:
4887 1252 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2541 2676 2998 3441 1186
Size: 1168 187 135 322 443 4139
Fragments arranged by size:
4139 1168 443 322 187 135
BseYI C'CCAG_C
Cuts at: 2095 2103 5597 6093 2095
Size: 8 3494 496 2396
Fragments arranged by size:
3494 2396 496 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2635 3663 1163
Size: 573 899 1028 3894
Fragments arranged by size:
3894 1028 899 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 3844 4605 5633 1540
Size: 275 186 35 1808 761 1028 2301
Fragments arranged by size:
2301 1808 1028 761 275 186 35
BsiHKAI G_wGCw'C
Cuts at: 2224 4455 4540 5611 2224
Size: 2231 85 1071 3007
Fragments arranged by size:
3007 2231 1071 85
BsmI GAATG_Cn'
Cuts at: 2399 3905 3998 2399
Size: 1506 93 4795
Fragments arranged by size:
4795 1506 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2102 2120 2224 2473 2648 3301
Size: 399 254 58 18 104 249 175 653
Cuts at: 3301 3335 3478 3528 3713 4455 4540 5611 1391
Size: 34 143 50 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 399 254 249 185
175 143 104 85 58 50 34 18
BspEI T'CCGG_A
Cuts at: 6057 6057
Size: 6394
BspHI T'CATG_A
Cuts at: 4283 6013 4283
Size: 1730 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 3749 844
Size: 1462 1443 3489
Fragments arranged by size:
3489 1462 1443
BsrBI CCG'CTC
Cuts at: 1394 1823 4281 1394
Size: 429 2458 3507
Fragments arranged by size:
3507 2458 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2800 3006 4889 5063 65
Size: 1183 1552 206 1883 174 1396
Fragments arranged by size:
1883 1552 1396 1183 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5038 5225 1700
Size: 110 3228 187 2869
Fragments arranged by size:
3228 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 2065 1925
Size: 140 6254
BssSI C'ACGA_G
Cuts at: 4454 5466 4454
Size: 1012 5382
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 2318 1184
Size: 1134 5260
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 3280 3448 2725
Size: 555 168 5671
Fragments arranged by size:
5671 555 168
BstYI r'GATC_y
Cuts at: 1972 2779 3724 4091 4476 4493 5945 6031 6043
Size: 807 945 367 385 17 1452 86 12
Cuts at: 6043 6389 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 945 807 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2018 2018
Size: 6394
BtgI C'CryG_G
Cuts at: 513 1065 2679 513
Size: 552 1614 4228
Fragments arranged by size:
4228 1614 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5655
Fragments arranged by size:
5655 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3910 4704 4724 2640
Size: 1270 794 20 4310
Fragments arranged by size:
4310 1270 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6359
DraI TTT'AAA
Cuts at: 3519 3698 3519
Size: 179 6215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5401 809
Size: 507 4085 1802
Fragments arranged by size:
4085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2578 3282
Size: 54 1425 50 275 18 203 545 704
Cuts at: 3282 3398 3450 3841 4730 6359 8
Size: 116 52 391 889 1629 43
Fragments arranged by size:
1629 1425 889 704 545 391 275 203
116 54 52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2033 3841 1537
Size: 275 221 1808 4090
Fragments arranged by size:
4090 1808 275 221
EarI CTCTTCn'nnn_
Cuts at: 1353 2371 2922 3540 4324 1353
Size: 1018 551 618 784 3423
Fragments arranged by size:
3423 1018 784 618 551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4981 5355 5501 387
Size: 1064 138 3392 374 146 1280
Fragments arranged by size:
3392 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2835 2946 3270 3481 3670 4457 5054
Size: 393 1203 111 324 211 189 787 597
Cuts at: 5054 5841 6109 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1203 787 787 597 393 324 268
211 189 111
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2129
Size: 6394
EcoO109I rG'GnC_Cy
Cuts at: 2732 2732
Size: 6394
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3004 3691 3004
Size: 687 5707
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 5541 1678
Size: 3863 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2270 2302 3586
Size: 32 135 32 530 32 136 32 1284
Cuts at: 3586 3618 5035 5067 5109 5141 1373
Size: 32 1417 32 42 32 2626
Fragments arranged by size:
2626 1417 1284 530 136 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3738 3984 669
Size: 988 281 1800 246 3079
Fragments arranged by size:
3079 1800 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 2718 3100 3719 2718
Size: 382 619 5393
Fragments arranged by size:
5393 619 382
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3094 5508 5692 1305
Size: 1789 2414 184 2007
Fragments arranged by size:
2414 2007 1789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3400 3452 10
Size: 54 1425 343 1568 52 2952
Fragments arranged by size:
2952 1568 1425 343 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 4352 4711 4870 518
Size: 648 3186 359 159 2042
Fragments arranged by size:
3186 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3287 3810 4487 5635 5880 1606
Size: 1681 523 677 1148 245 2120
Fragments arranged by size:
2120 1681 1148 677 523 245
NcoI C'CATG_G
Cuts at: 513 1065 2679 513
Size: 552 1614 4228
Fragments arranged by size:
4228 1614 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NsiI A_TGCA'T
Cuts at: 2399 2399
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 3752 5297 1132
Size: 97 2523 1545 2229
Fragments arranged by size:
2523 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2282 1348
Size: 934 5460
PciI A'CATG_T
Cuts at: 5293 5293
Size: 6394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2354 1108
Size: 1246 5148
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4463 4495 5976 6008 4463
Size: 32 1481 32 4849
Fragments arranged by size:
4849 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 2732
Size: 6394
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PspOMI G'GGCC_C
Cuts at: 2469 2469
Size: 6394
PstI C_TGCA'G
Cuts at: 830 3746 830
Size: 2916 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SapI GCTCTTCn'nnn_
Cuts at: 2922 2922
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SfcI C'TryA_G
Cuts at: 826 1045 3742 4877 5558 5749 826
Size: 219 2697 1135 681 191 1471
Fragments arranged by size:
2697 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3290 3290
Size: 6394
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3479 3721 1872
Size: 1607 242 4545
Fragments arranged by size:
4545 1607 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 4498 5399 5661 820
Size: 197 926 1035 1520 901 262 1553
Fragments arranged by size:
1553 1520 1035 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 2158 152
Size: 2006 4388
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 2620 3090 3190 4318 5
Size: 47 2568 470 100 1128 2081
Fragments arranged by size:
2568 2081 1128 470 100 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6394
StyI C'CwwG_G
Cuts at: 513 1065 2679 2739 3261 3833 513
Size: 552 1614 60 522 572 3074
Fragments arranged by size:
3074 1614 572 552 522 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 4421 4438 4591 4776 5221 325
Size: 1255 537 2304 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1255 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4640 6352 96
Size: 274 80 33 51 1443 2663 1712 138
Fragments arranged by size:
2663 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2655 2687 6354 6386 2655
Size: 32 3667 32 2663
Fragments arranged by size:
3667 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6394
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3622 3622
Size: 6394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3477 3719 1870
Size: 1607 242 4545
Fragments arranged by size:
4545 1607 242
XmnI GAAnn'nnTTC
Cuts at: 4523 4523
Size: 6394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI
SrfI SwaI