Restriction map for
pFN21AE6770
(Circular) (Six-base) MAPSORT of: pFN21AE6770.seq Check: 7458 from: 1 to: 6370
pFN21AE6770 6370 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2989 3644 2989
Size: 655 5715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2645 2985 4433 5817 6085 1632
Size: 901 112 340 1448 1384 268 1917
Fragments arranged by size:
1917 1448 1384 901 340 268 112
AflII C'TTAA_G
Cuts at: 820 1017 2173 820
Size: 197 1156 5017
Fragments arranged by size:
5017 1156 197
AflIII A'CryG_T
Cuts at: 1203 3089 3516 5269 6246 1203
Size: 1886 427 1753 977 1327
Fragments arranged by size:
1886 1753 1327 977 427
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 2297 2550 1166
Size: 1131 253 4986
Fragments arranged by size:
4986 1131 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3021 1184
Size: 1837 4533
AlwNI CAG_nnn'CTG
Cuts at: 2677 3074 3661 4113 5685 2677
Size: 397 587 452 1572 3362
Fragments arranged by size:
3362 1572 587 452 397
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 2249 2315 2324 2407 3079 3679 3905 4057
Size: 756 66 9 83 672 600 226 152
Cuts at: 4057 6166 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 756 672 600 226 152 83
66 9
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 3695 1870
Size: 73 1401 351 4545
Fragments arranged by size:
4545 1401 351 73
BamHI G'GATC_C
Cuts at: 3565 3700 4067 3565
Size: 135 367 5868
Fragments arranged by size:
5868 367 135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3691 5146 618
Size: 325 217 627 330 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2503 3324 3689 1391
Size: 949 163 821 365 4072
Fragments arranged by size:
4072 949 821 365 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 2410 2562 928
Size: 971 327 184 152 4736
Fragments arranged by size:
4736 971 327 184 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4561 4595 1903
Size: 34 50 34 2540 34 3678
Fragments arranged by size:
3678 2540 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2747 4262 5472 2747
Size: 1515 1210 3645
Fragments arranged by size:
3645 1515 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4981 136
Size: 107 122 71 631 605 3309 1525
Fragments arranged by size:
3309 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3056 6365 3056
Size: 3309 3061
BlpI GC'TnA_GC
Cuts at: 3067 3787 3067
Size: 720 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2616 2746 3251 3282 4431 5587 1790
Size: 826 130 505 31 1149 1156 2573
Fragments arranged by size:
2573 1156 1149 826 505 130 31
BmgBI CAC'GTC
Cuts at: 1318 2611 3252 3517 1318
Size: 1293 641 265 4171
Fragments arranged by size:
4171 1293 641 265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2466 4101 5059
Size: 416 53 83 399 24 1049 1635 958
Cuts at: 5059 442
Size: 1753
Fragments arranged by size:
1753 1635 1049 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2495 2930 3030 5030 1239
Size: 1124 132 435 100 2000 2579
Fragments arranged by size:
2579 2000 1124 435 132 100
Bpu10I CC'TnA_GC
Cuts at: 3487 3487
Size: 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 4495 5360 5658 2579
Size: 1916 865 298 3291
Fragments arranged by size:
3291 1916 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3633 5033 882
Size: 617 2134 1400 2219
Fragments arranged by size:
2219 2134 1400 617
BsaAI yAC'GTr
Cuts at: 493 1204 4161 493
Size: 711 2957 2702
Fragments arranged by size:
2957 2702 711
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4177 4559 5194 275
Size: 53 83 186 936 2644 382 635 1451
Fragments arranged by size:
2644 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3573 4803 5201 5475 5622 6033 3573
Size: 1230 398 274 147 411 3910
Fragments arranged by size:
3910 1230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3484 3514 3521 3551 1178
Size: 30 165 30 2081 30 7 30 3997
Fragments arranged by size:
3997 2081 165 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3275 1186
Size: 2089 4281
BseYI C'CCAG_C
Cuts at: 3600 5573 6069 3600
Size: 1973 496 3901
Fragments arranged by size:
3901 1973 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 3685 1163
Size: 573 452 1497 3848
Fragments arranged by size:
3848 1497 573 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 3820 4581 5609 1540
Size: 275 186 1573 246 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2340 4431 4516 5587 2340
Size: 2091 85 1071 3123
Fragments arranged by size:
3123 2091 1071 85
BsiWI C'GTAC_G
Cuts at: 2112 2112
Size: 6370
BsmI GAATG_Cn'
Cuts at: 2353 3272 3881 3974 2353
Size: 919 609 93 4749
Fragments arranged by size:
4749 919 609 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2601 2601
Size: 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2503 2616 2746 3251 3282 3324
Size: 399 550 163 113 130 505 31 42
Cuts at: 3324 3689 4431 4516 5587 1391
Size: 365 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 505 399 365 163
130 113 85 42 31
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 2748 2823 3309 4259 5989 2748
Size: 75 486 950 1730 3129
Fragments arranged by size:
3129 1730 950 486 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2989 3049 3644 3725 844
Size: 2145 60 595 81 3489
Fragments arranged by size:
3489 2145 595 81 60
BsrBI CCG'CTC
Cuts at: 1394 1823 3066 4257 1394
Size: 429 1243 1191 3507
Fragments arranged by size:
3507 1243 1191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2649 2892 3451 4865 5039 65
Size: 1183 1401 243 559 1414 174 1396
Fragments arranged by size:
1414 1401 1396 1183 559 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3150 5014 5201 1700
Size: 110 1340 1864 187 2869
Fragments arranged by size:
2869 1864 1340 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 4069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3504 794
Size: 2710 3660
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 4919
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 3056 3565 3700 4067 4452 4469 5921 6007
Size: 1084 509 135 367 385 17 1452 86
Cuts at: 6007 6019 6365 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1084 509 385 367 346 135
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2187 3111 2187
Size: 924 5446
BtgI C'CryG_G
Cuts at: 513 1065 3525 513
Size: 552 2460 3358
Fragments arranged by size:
3358 2460 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 3567 3650 3886 4680 4700 3505
Size: 62 83 236 794 20 5175
Fragments arranged by size:
5175 794 236 83 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3514 3549 529
Size: 35 2950 35 3350
Fragments arranged by size:
3350 2950 35 35
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DraIII CAC_nnn'GTG
Cuts at: 2865 2865
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3155 5377 809
Size: 507 1839 2222 1802
Fragments arranged by size:
2222 1839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3607 3817 4706
Size: 54 1425 50 275 18 1777 210 889
Cuts at: 4706 6335 8
Size: 1629 43
Fragments arranged by size:
1777 1629 1425 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3817 1537
Size: 275 2005 4090
Fragments arranged by size:
4090 2005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2435 3595 4300 1353
Size: 909 173 1160 705 3423
Fragments arranged by size:
3423 1160 909 705 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2093 2153 4957 5331 5477
Size: 1064 138 498 6 60 2804 374 146
Cuts at: 5477 387
Size: 1280
Fragments arranged by size:
2804 1280 1064 498 374 146 138 60
6
EcoICRI GAG'CTC
Cuts at: 2338 2338
Size: 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2495 2533 2645 2930 2985 3030
Size: 393 731 132 38 112 285 55 45
Cuts at: 3030 4433 5030 5817 6085 1239
Size: 1403 597 787 268 1524
Fragments arranged by size:
1524 1403 787 731 597 393 285 268
132 112 55 45 38
EcoO109I rG'GnC_Cy
Cuts at: 2425 2661 2796 3099 3553 2425
Size: 236 135 303 454 5242
Fragments arranged by size:
5242 454 303 236 135
EcoRI G'AATT_C
Cuts at: 2407 3679 4057 2407
Size: 1272 378 4720
Fragments arranged by size:
4720 1272 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 6370
FspI TGC'GCA
Cuts at: 2288 4876 2288
Size: 2588 3782
FspAI rTGC'GCAy
Cuts at: 2288 2288
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 5517 1678
Size: 3839 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 3325 3357 3385
Size: 32 135 32 949 32 772 32 28
Cuts at: 3385 3417 5011 5043 5085 5117 1373
Size: 32 1594 32 42 32 2626
Fragments arranged by size:
2626 1594 949 772 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3714 3960 669
Size: 988 281 1776 246 3079
Fragments arranged by size:
3079 1776 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3142 748
Size: 2394 3976
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3279 5484 5668 1305
Size: 1974 2205 184 2007
Fragments arranged by size:
2205 2007 1974 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3609 10
Size: 54 1425 343 1777 2771
Fragments arranged by size:
2771 1777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2297 2550 2753 2999 4328 4687
Size: 648 1047 84 253 203 246 1329 359
Cuts at: 4687 4846 518
Size: 159 2042
Fragments arranged by size:
2042 1329 1047 648 359 253 246 203
159 84
MspA1I CmG'CkG
Cuts at: 1606 2527 3245 3786 4463 5611 5856 1606
Size: 921 718 541 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 718 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5818
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 2202 2395 2404 2983 2998 3728 5273
Size: 97 973 193 9 579 15 730 1545
Cuts at: 5273 1132
Size: 2229
Fragments arranged by size:
2229 1545 973 730 579 193 97 15
9
PasI CC'CwG_GG
Cuts at: 1348 2729 1348
Size: 1381 4989
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PfoI T'CCnGG_A
Cuts at: 2340 2421 2508 3459 2340
Size: 81 87 951 5251
Fragments arranged by size:
5251 951 87 81
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2661 2425
Size: 236 6134
PshAI GACnn'nnGTC
Cuts at: 1613 2666 3519 1613
Size: 1053 853 4464
Fragments arranged by size:
4464 1053 853
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 3136 3598 3657 3722 830
Size: 2306 462 59 65 3478
Fragments arranged by size:
3478 2306 462 65 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2527 2527
Size: 6370
SacI G_AGCT'C
Cuts at: 2340 2340
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 3132 3594 3653 3718 4853 5534 5725
Size: 219 2087 462 59 65 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
2087 1471 1135 681 462 219 191 65
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2173 2594 4474 5375 5637 820
Size: 197 926 230 421 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 421 262 230 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 2395 2998 3728 1132
Size: 1263 603 730 3774
Fragments arranged by size:
3774 1263 730 603
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6370
StyI C'CwwG_G
Cuts at: 513 1065 2121 2712 3809 513
Size: 552 1056 591 1097 3074
Fragments arranged by size:
3074 1097 1056 591 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2923 3492 4397 4414 4567 4752 5197
Size: 1255 1343 569 905 17 153 185 445
Cuts at: 5197 325
Size: 1498
Fragments arranged by size:
1498 1343 1255 905 569 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 2760 4616
Size: 274 80 33 51 1443 420 363 1856
Cuts at: 4616 6328 96
Size: 1712 138
Fragments arranged by size:
1856 1712 1443 420 363 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3484 3503 3516 3535 6330 6362 3484
Size: 19 13 19 2795 32 3492
Fragments arranged by size:
3492 2795 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 4499 4499
Size: 6370
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BstZ17I EcoNI EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI