Restriction map for
pFN21AE6768
(Circular) (Six-base) MAPSORT of: pFN21AE6768.seq Check: 268 from: 1 to: 6301
pFN21AE6768 6301 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 6301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4364 5748 6016 1632
Size: 2732 1384 268 1917
Fragments arranged by size:
2732 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2140 2727 5200 6177 1203
Size: 937 587 2473 977 1327
Fragments arranged by size:
2473 1327 977 937 587
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2618 2650 2690 2722 2618
Size: 32 40 32 6197
Fragments arranged by size:
6197 40 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2360 4044 5616 2360
Size: 1684 1572 3045
Fragments arranged by size:
3045 1684 1572
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2254 2584 2737 3610 3836 3988 6097 1493
Size: 761 330 153 873 226 152 2109 1697
Fragments arranged by size:
2109 1697 873 761 330 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 2493 3231 4855 160
Size: 872 1461 738 1624 1606
Fragments arranged by size:
1624 1606 1461 872 738
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2683 3507 3626 1870
Size: 73 740 824 119 4545
Fragments arranged by size:
4545 824 740 119 73
BamHI G'GATC_C
Cuts at: 3370 3631 3998 3370
Size: 261 367 5673
Fragments arranged by size:
5673 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3622 5077 618
Size: 325 217 627 1835 1455 1842
Fragments arranged by size:
1842 1835 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2868 3134 3620 1391
Size: 697 780 266 486 4072
Fragments arranged by size:
4072 780 697 486 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 3385 928
Size: 971 933 553 3844
Fragments arranged by size:
3844 971 933 553
BbvCI CC'TCA_GC
Cuts at: 2639 2639
Size: 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2987 4193 5403 2987
Size: 1206 1210 3885
Fragments arranged by size:
3885 1210 1206
BclI T'GATC_A
Cuts at: 1548 2664 1548
Size: 1116 5185
BfrBI ATG'CAT
Cuts at: 2395 2395
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 4362 5518 1790
Size: 2572 1156 2573
Fragments arranged by size:
2573 2572 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2895 3249 3469
Size: 416 53 83 399 24 1478 354 220
Cuts at: 3469 4032 4990 442
Size: 563 958 1753
Fragments arranged by size:
1753 1478 958 563 416 399 354 220
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2180
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 4961 1239
Size: 863 2859 2579
Fragments arranged by size:
2859 2579 863
Bpu10I CC'TnA_GC
Cuts at: 2639 2639
Size: 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4964 882
Size: 617 3465 2219
Fragments arranged by size:
3465 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 3375 6295 3375
Size: 2920 3381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2648 4734 5132 5406 5553 5964 2648
Size: 2086 398 274 147 411 2985
Fragments arranged by size:
2985 2086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 1178
Size: 30 165 30 619 30 5427
Fragments arranged by size:
5427 619 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 1186
Size: 930 5371
BseYI C'CCAG_C
Cuts at: 2033 5504 6000 2033
Size: 3471 496 2334
Fragments arranged by size:
3471 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2425 1163
Size: 573 689 5039
Fragments arranged by size:
5039 689 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2868 4362 4447 5518 2868
Size: 1494 85 1071 3651
Fragments arranged by size:
3651 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2868 3134 3620 4362 4447 5518
Size: 399 298 780 266 486 742 85 1071
Cuts at: 5518 1391
Size: 2174
Fragments arranged by size:
2174 1071 780 742 486 399 298 266
85
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 2709 3216 3468 4190 5920 2709
Size: 507 252 722 1730 3090
Fragments arranged by size:
3090 1730 722 507 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 3656 844
Size: 1255 1557 3489
Fragments arranged by size:
3489 1557 1255
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2598 4796 4970 65
Size: 1183 1350 2198 174 1396
Fragments arranged by size:
2198 1396 1350 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 4945 5132 1700
Size: 110 713 2422 187 2869
Fragments arranged by size:
2869 2422 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 3060 3514 96
Size: 2964 454 2883
Fragments arranged by size:
2964 2883 454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2620 3444 2620
Size: 824 5477
BstYI r'GATC_y
Cuts at: 1972 3370 3631 3998 4383 4400 5852 5938 5950
Size: 1398 261 367 385 17 1452 86 12
Cuts at: 5950 6296 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1398 385 367 346 261 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2693 513
Size: 552 939 689 4121
Fragments arranged by size:
4121 939 689 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 4667
Fragments arranged by size:
4667 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2344 3817 4611 4631 2344
Size: 1473 794 20 4014
Fragments arranged by size:
4014 1473 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 3146 3181 529
Size: 35 1513 35 1034 35 3649
Fragments arranged by size:
3649 1513 1034 35 35 35
DraI TTT'AAA
Cuts at: 2503 3605 2503
Size: 1102 5199
DraIII CAC_nnn'GTG
Cuts at: 3212 3212
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 2978 5308 809
Size: 507 1245 417 2330 1802
Fragments arranged by size:
2330 1802 1245 507 417
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2371 3046
Size: 54 1425 50 275 18 177 364 675
Cuts at: 3046 3102 3748 4637 6266 8
Size: 56 646 889 1629 43
Fragments arranged by size:
1629 1425 889 675 646 364 275 177
56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2955 4231 1353
Size: 1041 561 1276 3423
Fragments arranged by size:
3423 1276 1041 561
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2639 4888 5262 5408 387
Size: 1064 138 1050 2249 374 146 1280
Fragments arranged by size:
2249 1280 1064 1050 374 146 138
EcoICRI GAG'CTC
Cuts at: 2866 2866
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 4364 4961 5748 6016 1239
Size: 393 470 2262 597 787 268 1524
Fragments arranged by size:
2262 1524 787 597 470 393 268
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 6301
FspI TGC'GCA
Cuts at: 3081 4807 3081
Size: 1726 4575
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2649 2681 2865
Size: 32 135 32 450 32 595 32 184
Cuts at: 2865 2897 3512 3544 4942 4974 5016 5048 1373
Size: 32 615 32 1398 32 42 32 2626
Fragments arranged by size:
2626 1398 615 595 450 184 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 2754 3900 2754
Size: 1146 5155
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 5415 5599 1305
Size: 903 3207 184 2007
Fragments arranged by size:
3207 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2373 3048 3104 10
Size: 54 1425 343 177 364 675 56 3207
Fragments arranged by size:
3207 1425 675 364 343 177 56 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 4259 4618 4777 518
Size: 648 1437 1656 359 159 2042
Fragments arranged by size:
2042 1656 1437 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2190 2839 3717 4394 5542 5787 1606
Size: 437 147 649 878 677 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 649 437 245 147
NaeI GCC'GGC
Cuts at: 2525 2525
Size: 6301
NcoI C'CATG_G
Cuts at: 513 1065 2004 2693 513
Size: 552 939 689 4121
Fragments arranged by size:
4121 939 689 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NgoMIV G'CCGG_C
Cuts at: 2523 2523
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NsiI A_TGCA'T
Cuts at: 2397 2397
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2144 2731 3659 5204 1132
Size: 97 915 587 928 1545 2229
Fragments arranged by size:
2229 1545 928 915 587 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 2140 2727 5200 2140
Size: 587 2473 3241
Fragments arranged by size:
3241 2473 587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2330 1613
Size: 717 5584
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspXI vC'TCGA_Gb
Cuts at: 3507 3507
Size: 6301
PstI C_TGCA'G
Cuts at: 830 2339 3653 830
Size: 1509 1314 3478
Fragments arranged by size:
3478 1509 1314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2190 2839 2190
Size: 649 5652
SacI G_AGCT'C
Cuts at: 2868 2868
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SapI GCTCTTCn'nnn_
Cuts at: 2955 2955
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 2513 4549 6261 2513
Size: 2036 1712 2553
Fragments arranged by size:
2553 2036 1712
SfcI C'TryA_G
Cuts at: 826 1045 2335 3027 3144 3649 4784 5465 5656
Size: 219 1290 692 117 505 1135 681 191
Cuts at: 5656 826
Size: 1471
Fragments arranged by size:
1471 1290 1135 692 681 505 219 191
117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3507 4405 5306 5568 820
Size: 197 926 1564 898 901 262 1553
Fragments arranged by size:
1564 1553 926 901 898 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 3053 1517
Size: 1536 4765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 2693 3126 3740 513
Size: 552 939 109 580 433 614 3074
Fragments arranged by size:
3074 939 614 580 552 433 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 3498 4328 4345 4498 4683 5128
Size: 1255 1070 848 830 17 153 185 445
Cuts at: 5128 325
Size: 1498
Fragments arranged by size:
1498 1255 1070 848 830 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3060 3514
Size: 274 80 33 51 1443 534 549 454
Cuts at: 3514 4547 6259 96
Size: 1033 1712 138
Fragments arranged by size:
1712 1443 1033 549 534 454 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2760 2760
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 3507 1943
Size: 1564 4737
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2894 3543 4430 2894
Size: 649 887 4765
Fragments arranged by size:
4765 887 649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI