Restriction map for
pFN21AE6766
(Circular) (Six-base) MAPSORT of: pFN21AE6766.seq Check: 3597 from: 1 to: 6475
pFN21AE6766 6475 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3574 3574
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3024 3818 1937
Size: 1087 794 4594
Fragments arranged by size:
4594 1087 794
Acc65I G'GTAC_C
Cuts at: 3175 3796 3175
Size: 621 5854
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 3710 4538 5922 6190 1632
Size: 2006 72 828 1384 268 1917
Fragments arranged by size:
2006 1917 1384 828 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 2311 2715 5374 6351 1203
Size: 1108 404 2659 977 1327
Fragments arranged by size:
2659 1327 1108 977 404
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 2024 3397 5204 2024
Size: 1373 1807 3295
Fragments arranged by size:
3295 1807 1373
AleI CACnn'nnGTG
Cuts at: 1166 3348 1166
Size: 2182 4293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2238 2607 1184
Size: 1054 369 5052
Fragments arranged by size:
5052 1054 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2423 2455 2423
Size: 32 6443
AlwNI CAG_nnn'CTG
Cuts at: 2523 3449 4218 5790 2523
Size: 926 769 1572 3208
Fragments arranged by size:
3208 1572 926 769
ApaLI G'TGCA_C
Cuts at: 2046 2879 4532 5688 2046
Size: 833 1653 1156 2833
Fragments arranged by size:
2833 1653 1156 833
ApoI r'AATT_y
Cuts at: 1493 3332 3784 4010 4162 6271 1493
Size: 1839 452 226 152 2109 1697
Fragments arranged by size:
2109 1839 1697 452 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 3753 3800 1870
Size: 73 1810 47 4545
Fragments arranged by size:
4545 1810 73 47
BamHI G'GATC_C
Cuts at: 2795 3378 3805 4172 2795
Size: 583 427 367 5098
Fragments arranged by size:
5098 583 427 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2418 2915 3175 3796 5251
Size: 325 217 627 631 497 260 621 1455
Cuts at: 5251 618
Size: 1842
Fragments arranged by size:
1842 1455 631 627 621 497 325 260
217
BanII G_rGCy'C
Cuts at: 1391 3579 3794 1391
Size: 2188 215 4072
Fragments arranged by size:
4072 2188 215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 928
Size: 971 121 5383
Fragments arranged by size:
5383 971 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2168 2202 3466 3500 4666 4700 1903
Size: 34 231 34 1264 34 1166 34 3678
Fragments arranged by size:
3678 1264 1166 231 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2384 4367 5577 2384
Size: 1983 1210 3282
Fragments arranged by size:
3282 1983 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5086 136
Size: 107 122 71 631 605 3414 1525
Fragments arranged by size:
3414 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2134 6470 2134
Size: 4336 2139
BlpI GC'TnA_GC
Cuts at: 2627 3892 2627
Size: 1265 5210
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2757 2883 4536 5692 1790
Size: 260 707 126 1653 1156 2573
Fragments arranged by size:
2573 1653 1156 707 260 126
BmgBI CAC'GTC
Cuts at: 1318 2312 2718 3684 1318
Size: 994 406 966 4109
Fragments arranged by size:
4109 994 966 406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 4206 5164
Size: 416 53 83 399 24 1323 1466 958
Cuts at: 5164 442
Size: 1753
Fragments arranged by size:
1753 1466 1323 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2977 3272 3760 2187
Size: 790 295 488 4902
Fragments arranged by size:
4902 790 488 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2692 3517 5135 1239
Size: 1453 825 1618 2579
Fragments arranged by size:
2579 1618 1453 825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2011 2644 3279 5138 882
Size: 617 512 633 635 1859 2219
Fragments arranged by size:
2219 1859 635 633 617 512
BsaAI yAC'GTr
Cuts at: 493 1204 2878 4266 493
Size: 711 1674 1388 2702
Fragments arranged by size:
2702 1674 1388 711
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 4282 4664 5299
Size: 53 83 186 936 635 2114 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
2114 1451 936 635 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 3338 3368 3480
Size: 30 165 30 1020 30 885 30 112
Cuts at: 3480 3510 1178
Size: 30 4143
Fragments arranged by size:
4143 1020 885 165 112 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2732 1186
Size: 1546 4929
BseYI C'CCAG_C
Cuts at: 2634 3126 5678 6174 2634
Size: 492 2552 496 2935
Fragments arranged by size:
2935 2552 496 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 3347 1163
Size: 573 867 744 4291
Fragments arranged by size:
4291 867 744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2182 3283 3925 4686 5714 1540
Size: 275 186 181 1101 642 761 1028 2301
Fragments arranged by size:
2301 1101 1028 761 642 275 186 181
BsiHKAI G_wGCw'C
Cuts at: 2050 2883 4536 4621 5692 2050
Size: 833 1653 85 1071 2833
Fragments arranged by size:
2833 1653 1071 833 85
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2757 2883 3579 3794 4536 4621
Size: 399 260 707 126 696 215 742 85
Cuts at: 4621 5692 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 707 696 399 260 215
126 85
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3369 3574 3830 844
Size: 2525 205 256 3489
Fragments arranged by size:
3489 2525 256 205
BsrBI CCG'CTC
Cuts at: 1394 1823 2631 4362 1394
Size: 429 808 1731 3507
Fragments arranged by size:
3507 1731 808 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2158 2595 4970 5144 65
Size: 1183 910 437 2375 174 1396
Fragments arranged by size:
2375 1396 1183 910 437 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 5119 5306 1700
Size: 110 437 2872 187 2869
Fragments arranged by size:
2872 2869 437 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 2756 4535 5547 2756
Size: 1779 1012 3684
Fragments arranged by size:
3684 1779 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3370 794
Size: 2576 3899
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 3496 1184
Size: 2312 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 3083 3350 3083
Size: 267 6208
BstYI r'GATC_y
Cuts at: 1972 2134 2795 3378 3805 4172 4557 4574 6026
Size: 162 661 583 427 367 385 17 1452
Cuts at: 6026 6112 6124 6470 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 661 583 427 385 367 346
162 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3005 3261 3005
Size: 256 6219
BtgI C'CryG_G
Cuts at: 513 1065 2898 2964 513
Size: 552 1833 66 4024
Fragments arranged by size:
4024 1833 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 3991 4785 4805 3991
Size: 794 20 5661
Fragments arranged by size:
5661 794 20
ClaI AT'CG_AT
Cuts at: 1554 2991 1554
Size: 1437 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DraIII CAC_nnn'GTG
Cuts at: 2077 3370 3421 2077
Size: 1293 51 5131
Fragments arranged by size:
5131 1293 51
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2525 3280 3606
Size: 54 1425 50 275 18 695 755 326
Cuts at: 3606 3922 4811 6440 8
Size: 316 889 1629 43
Fragments arranged by size:
1629 1425 889 755 695 326 316 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 3922 1537
Size: 275 1468 642 4090
Fragments arranged by size:
4090 1468 642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3527 3729 3764 4405 1353
Size: 1593 581 202 35 641 3423
Fragments arranged by size:
3423 1593 641 581 202 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5062 5436 5582 387
Size: 1064 138 3473 374 146 1280
Fragments arranged by size:
3473 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2692 3517 3638 3710 4538 5135 5922
Size: 393 1060 825 121 72 828 597 787
Cuts at: 5922 6190 1239
Size: 268 1524
Fragments arranged by size:
1524 1060 828 825 787 597 393 268
121 72
EcoO109I rG'GnC_Cy
Cuts at: 3538 3758 3538
Size: 220 6255
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 6475
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 5622 1678
Size: 3944 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2262 2294 2301
Size: 32 135 32 438 32 220 32 7
Cuts at: 2301 2333 3383 3415 5116 5148 5190 5222 1373
Size: 32 1050 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 1050 438 220 135 42 32
32 32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2515 3819 4065 669
Size: 988 281 577 1304 246 3079
Fragments arranged by size:
3079 1304 988 577 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6475
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KpnI G_GTAC'C
Cuts at: 3179 3800 3179
Size: 621 5854
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5589 5773 1305
Size: 4284 184 2007
Fragments arranged by size:
4284 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3608 10
Size: 54 1425 343 1776 2877
Fragments arranged by size:
2877 1776 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 3348 4433 4792 4951 518
Size: 648 1638 544 1085 359 159 2042
Fragments arranged by size:
2042 1638 1085 648 544 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 3182 3891 4568 5716 5961 1606
Size: 914 662 709 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 709 677 662 245
NcoI C'CATG_G
Cuts at: 513 1065 2964 513
Size: 552 1899 4024
Fragments arranged by size:
4024 1899 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2064 2500 3074 3833 5378 1132
Size: 97 835 436 574 759 1545 2229
Fragments arranged by size:
2229 1545 835 759 574 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3449 1108
Size: 2341 4134
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PmlI CAC'GTG
Cuts at: 2878 2878
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 2455 4544 4576 6057 6089 2423
Size: 32 2089 32 1481 32 2809
Fragments arranged by size:
2809 2089 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3538 3758 3538
Size: 220 6255
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PstI C_TGCA'G
Cuts at: 830 2240 3306 3827 830
Size: 1410 1066 521 3478
Fragments arranged by size:
3478 1410 1066 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2520 3182 2520
Size: 662 5813
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SanDI GG'GwC_CC
Cuts at: 3538 3538
Size: 6475
SapI GCTCTTCn'nnn_
Cuts at: 3527 3527
Size: 6475
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2236 3302 3823 4958 5639 5830 826
Size: 219 1191 1066 521 1135 681 191 1471
Fragments arranged by size:
1471 1191 1135 1066 681 521 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 5480 5742 820
Size: 197 926 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 2500 3074 3833 1132
Size: 1368 574 759 3774
Fragments arranged by size:
3774 1368 759 574
SspI AAT'ATT
Cuts at: 5 52 2098 4399 5
Size: 47 2046 2301 2081
Fragments arranged by size:
2301 2081 2046 47
StuI AGG'CCT
Cuts at: 1517 2343 1517
Size: 826 5649
StyI C'CwwG_G
Cuts at: 513 1065 2022 2422 2964 3384 3914 513
Size: 552 957 400 542 420 530 3074
Fragments arranged by size:
3074 957 552 542 530 420 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4502 4519 4672 4857 5302 325
Size: 1255 2922 17 153 185 445 1498
Fragments arranged by size:
2922 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 3269 1316
Size: 1953 4522
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2020
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI SacI SacII SexAI SfiI
SfoI SrfI SwaI