Restriction map for
pFN21AE6764
(Circular) (Six-base) MAPSORT of: pFN21AE6764.seq Check: 7608 from: 1 to: 7555
pFN21AE6764 7555 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5365 278
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4898 1937
Size: 2961 4594
Acc65I G'GTAC_C
Cuts at: 3011 4876 3011
Size: 1865 5690
AclI AA'CG_TT
Cuts at: 1563 5682 6055 1563
Size: 4119 373 3063
Fragments arranged by size:
4119 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 4208 5618 7002 7270 1632
Size: 642 1934 1410 1384 268 1917
Fragments arranged by size:
1934 1917 1410 1384 642 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7358
AflIII A'CryG_T
Cuts at: 1203 3099 3596 6454 7431 1203
Size: 1896 497 2858 977 1327
Fragments arranged by size:
2858 1896 1327 977 497
AgeI A'CCGG_T
Cuts at: 6386 6386
Size: 7555
AhdI GACnn_n'nnGTC
Cuts at: 4460 6284 4460
Size: 1824 5731
AleI CACnn'nnGTG
Cuts at: 1166 4298 4759 1166
Size: 3132 461 3962
Fragments arranged by size:
3962 3132 461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4226 4289 4595 1184
Size: 3042 63 306 4144
Fragments arranged by size:
4144 3042 306 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 3980 4012 2207
Size: 32 1741 32 5750
Fragments arranged by size:
5750 1741 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2965 4707 4828 5298 6870 2965
Size: 1742 121 470 1572 3650
Fragments arranged by size:
3650 1742 1572 470 121
ApaI G_GGCC'C
Cuts at: 2566 2841 2566
Size: 275 7280
ApaLI G'TGCA_C
Cuts at: 3412 3631 5612 6768 3412
Size: 219 1981 1156 4199
Fragments arranged by size:
4199 1981 1156 219
ApoI r'AATT_y
Cuts at: 1493 4864 5090 5242 7351 1493
Size: 3371 226 152 2109 1697
Fragments arranged by size:
3371 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2629 2629
Size: 7555
AseI AT'TA_AT
Cuts at: 160 1032 6109 160
Size: 872 5077 1606
Fragments arranged by size:
5077 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2536 2924 3305 3840 4380 4416
Size: 73 195 398 388 381 535 540 36
Cuts at: 4416 4449 4791 4880 1870
Size: 33 342 89 4545
Fragments arranged by size:
4545 540 535 398 388 381 342 195
89 73 36 33
BamHI G'GATC_C
Cuts at: 3571 4885 5252 3571
Size: 1314 367 5874
Fragments arranged by size:
5874 1314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 3011 3320 3473 3814
Size: 325 217 627 609 615 309 153 341
Cuts at: 3814 3898 4118 4876 6331 618
Size: 84 220 758 1455 1842
Fragments arranged by size:
1842 1455 758 627 615 609 341 325
309 220 217 153 84
BanII G_rGCy'C
Cuts at: 1391 2277 2288 2373 2566 2841 3371 3711 3998
Size: 886 11 85 193 275 530 340 287
Cuts at: 3998 4610 4683 4701 4874 1391
Size: 612 73 18 173 4072
Fragments arranged by size:
4072 886 612 530 340 287 275 193
173 85 73 18 11
BbeI G_GCGC'C
Cuts at: 2400 3324 4122 2400
Size: 924 798 5833
Fragments arranged by size:
5833 924 798
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6584
BbvCI CC'TCA_GC
Cuts at: 4481 4481
Size: 7555
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5746 5780 1903
Size: 34 50 34 3725 34 3678
Fragments arranged by size:
3725 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5447 6657 5447
Size: 1210 6345
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7555
BfrBI ATG'CAT
Cuts at: 4084 4084
Size: 7555
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4776 6166 136
Size: 107 122 71 631 605 3104 1390 1525
Fragments arranged by size:
3104 1525 1390 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7550 7550
Size: 7555
BlpI GC'TnA_GC
Cuts at: 3721 4700 4972 3721
Size: 979 272 6304
Fragments arranged by size:
6304 979 272
Bme1580I G_kGCm'C
Cuts at: 1790 2566 2841 3261 3416 3635 3819 4295 5616
Size: 776 275 420 155 219 184 476 1321
Cuts at: 5616 6772 1790
Size: 1156 2573
Fragments arranged by size:
2573 1321 1156 776 476 420 275 219
184 155
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 6550
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2941 5286 6244
Size: 416 53 83 399 24 1524 2345 958
Cuts at: 6244 442
Size: 1753
Fragments arranged by size:
2345 1753 1524 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2433 1056
Size: 1377 6178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 4372 2356
Size: 2016 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2252 2459 3725 4352 6215 1239
Size: 834 179 207 1266 627 1863 2579
Fragments arranged by size:
2579 1863 1266 834 627 207 179
Bpu10I CC'TnA_GC
Cuts at: 3335 4481 3335
Size: 1146 6409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4742 5680 6545 6843 4108
Size: 634 938 865 298 4820
Fragments arranged by size:
4820 938 865 634 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2502 6218 882
Size: 617 1003 3716 2219
Fragments arranged by size:
3716 2219 1003 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 3100 5346 493
Size: 711 1458 438 2246 2702
Fragments arranged by size:
2702 2246 1458 711 438
BsaBI GATnn'nnATC
Cuts at: 7549 7549
Size: 7555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2259 2397 3321
Size: 53 83 186 936 677 49 138 924
Cuts at: 3321 4119 5362 5744 6379 275
Size: 798 1243 382 635 1451
Fragments arranged by size:
1451 1243 936 924 798 677 635 382
186 138 83 53 49
BsaWI w'CCGG_w
Cuts at: 2504 3728 5988 6386 6660 6807 7218 2504
Size: 1224 2260 398 274 147 411 2841
Fragments arranged by size:
2841 2260 1224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2457 2487 3666 3696 3737
Size: 30 165 30 1054 30 1179 30 41
Cuts at: 3737 3767 3926 3956 3980 4010 4412 4442 1178
Size: 30 159 30 24 30 402 30 4291
Fragments arranged by size:
4291 1179 1054 402 165 159 41 30
30 30 30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3152 3761 1186
Size: 1966 609 4980
Fragments arranged by size:
4980 1966 609
BseYI C'CCAG_C
Cuts at: 2014 2771 3091 3284 3716 4530 4545 6758 7254
Size: 757 320 193 432 814 15 2213 496
Cuts at: 7254 2014
Size: 2315
Fragments arranged by size:
2315 2213 814 757 496 432 320 193
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3382 3632 4509 1163
Size: 573 1646 250 877 4209
Fragments arranged by size:
4209 1646 877 573 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3199 4735 5005 5766 6794
Size: 275 186 115 1083 1536 270 761 1028
Cuts at: 6794 1540
Size: 2301
Fragments arranged by size:
2301 1536 1083 1028 761 275 270 186
115
BsiHKAI G_wGCw'C
Cuts at: 3416 3635 3929 4559 4701 5616 5701 6772 3416
Size: 219 294 630 142 915 85 1071 4199
Fragments arranged by size:
4199 1071 915 630 294 219 142 85
BsmI GAATG_Cn'
Cuts at: 5066 5159 5066
Size: 93 7462
BsmBI CGTCTCn'nnnn_
Cuts at: 2660 2660
Size: 7555
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2288 2373 2566 2841 3261 3371
Size: 399 487 11 85 193 275 420 110
Cuts at: 3371 3416 3635 3711 3819 3929 3998 4295 4559
Size: 45 219 76 108 110 69 297 264
Cuts at: 4559 4610 4683 4701 4874 5616 5701 6772 1391
Size: 51 73 18 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 420 399 297 275
264 219 193 173 110 110 108 85
85 76 73 69 51 45 18 11
BspEI T'CCGG_A
Cuts at: 2504 3728 7218 2504
Size: 1224 3490 2841
Fragments arranged by size:
3490 2841 1224
BspHI T'CATG_A
Cuts at: 5444 7174 5444
Size: 1730 5825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 2610 4218 4910 844
Size: 1460 306 1608 692 3489
Fragments arranged by size:
3489 1608 1460 692 306
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 4246 5442 1394
Size: 429 248 2175 1196 3507
Fragments arranged by size:
3507 2175 1196 429 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 6050 6224 65
Size: 1183 1612 3190 174 1396
Fragments arranged by size:
3190 1612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2437 2613 2849 3789 4779 6199 6386
Size: 110 627 176 236 940 990 1420 187
Cuts at: 6386 1700
Size: 2869
Fragments arranged by size:
2869 1420 990 940 627 236 187 176
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7555
BssHII G'CGCG_C
Cuts at: 1925 2433 2629 1925
Size: 508 196 6851
Fragments arranged by size:
6851 508 196
BssSI C'ACGA_G
Cuts at: 3086 5615 6627 3086
Size: 2529 1012 4014
Fragments arranged by size:
4014 2529 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3498 3666 3924 794
Size: 2704 168 258 4425
Fragments arranged by size:
4425 2704 258 168
BstBI TT'CG_AA
Cuts at: 6359 6359
Size: 7555
BstEII G'GTnAC_C
Cuts at: 1184 2845 4710 1184
Size: 1661 1865 4029
Fragments arranged by size:
4029 1865 1661
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 3910 4498 2095
Size: 1815 588 5152
Fragments arranged by size:
5152 1815 588
BstYI r'GATC_y
Cuts at: 1972 3310 3571 4885 5252 5637 5654 7106 7192
Size: 1338 261 1314 367 385 17 1452 86
Cuts at: 7192 7204 7550 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1338 1314 385 367 346 261
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4844 4844
Size: 7555
BtgI C'CryG_G
Cuts at: 513 1065 2374 2582 2654 2704 3804 4344 4359
Size: 552 1309 208 72 50 1100 540 15
Cuts at: 4359 4656 4771 513
Size: 297 115 3297
Fragments arranged by size:
3297 1309 1100 552 540 297 208 115
72 50 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6816
Fragments arranged by size:
6816 683 56
BtsI GCAGTG_nn'
Cuts at: 2937 3078 3229 3350 3635 3758 4579 5071 5865
Size: 141 151 121 285 123 821 492 794
Cuts at: 5865 5885 2937
Size: 20 4607
Fragments arranged by size:
4607 821 794 492 285 151 141 123
121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7555
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7520
DraI TTT'AAA
Cuts at: 4859 4859
Size: 7555
DraIII CAC_nnn'GTG
Cuts at: 3547 3924 3547
Size: 377 7178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4838 6562 809
Size: 507 3522 1724 1802
Fragments arranged by size:
3522 1802 1724 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 3618 4104
Size: 54 1425 50 275 18 283 1505 486
Cuts at: 4104 4732 5002 5891 7520 8
Size: 628 270 889 1629 43
Fragments arranged by size:
1629 1505 1425 889 628 486 283 275
270 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 4732 5002 1537
Size: 275 301 2619 270 4090
Fragments arranged by size:
4090 2619 301 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 2293 2954 3998 4073 5485 1353
Size: 940 661 1044 75 1412 3423
Fragments arranged by size:
3423 1412 1044 940 661 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4127 4600 6142 6516 6662 387
Size: 1064 138 2538 473 1542 374 146 1280
Fragments arranged by size:
2538 1542 1280 1064 473 374 146 138
EcoICRI GAG'CTC
Cuts at: 4699 4699
Size: 7555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2252 2274 2459 3725 4208 4352
Size: 393 441 179 22 185 1266 483 144
Cuts at: 4352 5618 6215 7002 7270 1239
Size: 1266 597 787 268 1524
Fragments arranged by size:
1524 1266 1266 787 597 483 441 393
268 185 179 144 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3305 3305
Size: 7555
EcoO109I rG'GnC_Cy
Cuts at: 2135 2563 2962 3510 4249 4351 4444 4454 4518
Size: 428 399 548 739 102 93 10 64
Cuts at: 4518 2135
Size: 5172
Fragments arranged by size:
5172 739 548 428 399 102 93 64
10
EcoRI G'AATT_C
Cuts at: 4864 5242 4864
Size: 378 7177
FspI TGC'GCA
Cuts at: 2873 6061 2873
Size: 3188 4367
HaeII r_GCGC'y
Cuts at: 1678 2400 2482 3324 4122 4707 4753 6702 1678
Size: 722 82 842 798 585 46 1949 2531
Fragments arranged by size:
2531 1949 842 798 722 585 82 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2816 2848 3516
Size: 32 135 32 929 32 283 32 668
Cuts at: 3516 3548 3926 3958 4412 4415 4444 4446 4447
Size: 32 378 32 454 3 29 2 1
Cuts at: 4447 4478 6196 6228 6270 6302 1373
Size: 31 1718 32 42 32 2626
Fragments arranged by size:
2626 1718 929 668 454 378 283 135
42 32 32 32 32 32 32 32
32 31 29 3 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4899 5145 669
Size: 988 281 2961 246 3079
Fragments arranged by size:
3079 2961 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7555
HpaI GTT'AAC
Cuts at: 5145 5145
Size: 7555
KasI G'GCGC_C
Cuts at: 2396 3320 4118 2396
Size: 924 798 5833
Fragments arranged by size:
5833 924 798
KpnI G_GTAC'C
Cuts at: 3015 4880 3015
Size: 1865 5690
MfeI C'AATT_G
Cuts at: 5154 5154
Size: 7555
MluI A'CGCG_T
Cuts at: 7431 7431
Size: 7555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2116 4515 6669 6853 1305
Size: 811 2399 2154 184 2007
Fragments arranged by size:
2399 2154 2007 811 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3620 4106 10
Size: 54 1425 343 1788 486 3459
Fragments arranged by size:
3459 1788 1425 486 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 3120 4298 4759 5513 5872 6031
Size: 648 1632 322 1178 461 754 359 159
Cuts at: 6031 518
Size: 2042
Fragments arranged by size:
2042 1632 1178 754 648 461 359 322
159
MspA1I CmG'CkG
Cuts at: 1606 2051 2376 2419 2584 2656 3255 3480 3616
Size: 445 325 43 165 72 599 225 136
Cuts at: 3616 4284 4298 4339 4545 4971 5648 6796 7041
Size: 668 14 41 206 426 677 1148 245
Cuts at: 7041 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 668 599 445 426 325
245 225 206 165 136 72 43 41
14
NaeI GCC'GGC
Cuts at: 2439 2615 4781 2439
Size: 176 2166 5213
Fragments arranged by size:
5213 2166 176
NarI GG'CG_CC
Cuts at: 2397 3321 4119 2397
Size: 924 798 5833
Fragments arranged by size:
5833 924 798
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7003
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7555
NgoMIV G'CCGG_C
Cuts at: 2437 2613 4779 2437
Size: 176 2166 5213
Fragments arranged by size:
5213 2166 176
NheI G'CTAG_C
Cuts at: 1052 2429 1052
Size: 1377 6178
NotI GC'GGCC_GC
Cuts at: 5002 5002
Size: 7555
NsiI A_TGCA'T
Cuts at: 4086 4086
Size: 7555
NspI r_CATG'y
Cuts at: 1132 1229 3112 3391 3600 4913 6458 1132
Size: 97 1883 279 209 1313 1545 2229
Fragments arranged by size:
2229 1883 1545 1313 279 209 97
PasI CC'CwG_GG
Cuts at: 1348 2992 3462 1348
Size: 1644 470 5441
Fragments arranged by size:
5441 1644 470
PciI A'CATG_T
Cuts at: 3596 6454 3596
Size: 2858 4697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3091 3210 3408 4828 1108
Size: 1983 119 198 1420 3835
Fragments arranged by size:
3835 1983 1420 198 119
PfoI T'CCnGG_A
Cuts at: 3246 3273 3741 4329 3246
Size: 27 468 588 6472
Fragments arranged by size:
6472 588 468 27
PmeI GTTT'AAAC
Cuts at: 4859 4859
Size: 7555
PmlI CAC'GTG
Cuts at: 3100 3100
Size: 7555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5624 5656 7137 7169 3980
Size: 32 1612 32 1481 32 4366
Fragments arranged by size:
4366 1612 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2962 4249 4351 4444 4454 2135
Size: 827 1287 102 93 10 5236
Fragments arranged by size:
5236 1287 827 102 93 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7555
PsiI TTA'TAA
Cuts at: 5125 5125
Size: 7555
PspOMI G'GGCC_C
Cuts at: 2562 2837 2562
Size: 275 7280
PstI C_TGCA'G
Cuts at: 830 3224 4314 4584 4907 830
Size: 2394 1090 270 323 3478
Fragments arranged by size:
3478 2394 1090 323 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7555
PvuII CAG'CTG
Cuts at: 3480 3616 4545 3480
Size: 136 929 6490
Fragments arranged by size:
6490 929 136
SacI G_AGCT'C
Cuts at: 4701 4701
Size: 7555
SacII CC_GC'GG
Cuts at: 2377 2585 2657 2377
Size: 208 72 7275
Fragments arranged by size:
7275 208 72
SalI G'TCGA_C
Cuts at: 1936 4897 1936
Size: 2961 4594
SanDI GG'GwC_CC
Cuts at: 2135 4454 2135
Size: 2319 5236
SapI GCTCTTCn'nnn_
Cuts at: 2293 2293
Size: 7555
SbfI CC_TGCA'GG
Cuts at: 4314 4907 4314
Size: 593 6962
ScaI AGT'ACT
Cuts at: 2930 3395 3665 3824 5803 7515 2930
Size: 465 270 159 1979 1712 2970
Fragments arranged by size:
2970 1979 1712 465 270 159
SexAI A'CCwGG_T
Cuts at: 4251 4494 4251
Size: 243 7312
SfcI C'TryA_G
Cuts at: 826 1045 3220 4310 4580 4903 6038 6719 6910
Size: 219 2175 1090 270 323 1135 681 191
Cuts at: 6910 826
Size: 1471
Fragments arranged by size:
2175 1471 1135 1090 681 323 270 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4776 4776
Size: 7555
SfoI GGC'GCC
Cuts at: 2398 3322 4120 2398
Size: 924 798 5833
Fragments arranged by size:
5833 924 798
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7555
SgrAI Cr'CCGG_yG
Cuts at: 1700 2437 1700
Size: 737 6818
SmaI CCC'GGG
Cuts at: 1872 3842 4382 4451 4793 4882 1872
Size: 1970 540 69 342 89 4545
Fragments arranged by size:
4545 1970 540 342 89 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4123 4757 5659 6560 6822 820
Size: 197 926 2180 634 902 901 262 1553
Fragments arranged by size:
2180 1553 926 902 901 634 262 197
SnaBI TAC'GTA
Cuts at: 493 5346 493
Size: 4853 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7555
SphI G_CATG'C
Cuts at: 1132 4913 1132
Size: 3781 3774
SspI AAT'ATT
Cuts at: 5 52 5479 5
Size: 47 5427 2081
Fragments arranged by size:
5427 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7555
StyI C'CwwG_G
Cuts at: 513 1065 2841 3426 3903 3955 4198 4440 4994
Size: 552 1776 585 477 52 243 242 554
Cuts at: 4994 513
Size: 3074
Fragments arranged by size:
3074 1776 585 554 552 477 243 242
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3802 3916 4522 5582 5599 5752 5937
Size: 1255 2222 114 606 1060 17 153 185
Cuts at: 5937 6382 325
Size: 445 1498
Fragments arranged by size:
2222 1498 1255 1060 606 445 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2928 3393 3663
Size: 274 80 33 51 1443 951 465 270
Cuts at: 3663 3822 5801 7513 96
Size: 159 1979 1712 138
Fragments arranged by size:
1979 1712 1443 951 465 274 270 159
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 3158 3190 3423 3455 3666 3698 3737
Size: 32 669 32 233 32 211 32 39
Cuts at: 3737 3769 7515 7547 2457
Size: 32 3746 32 2465
Fragments arranged by size:
3746 2465 669 233 211 39 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7555
XbaI T'CTAG_A
Cuts at: 4891 4891
Size: 7555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2251 3433 2011
Size: 240 1182 6133
Fragments arranged by size:
6133 1182 240
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7555
XmaI C'CCGG_G
Cuts at: 1870 3840 4380 4449 4791 4880 1870
Size: 1970 540 69 342 89 4545
Fragments arranged by size:
4545 1970 540 342 89 69
XmnI GAAnn'nnTTC
Cuts at: 4007 5684 4007
Size: 1677 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5363 276
Size: 53 83 186 936 3829 2468
Fragments arranged by size:
3829 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AvrII BaeI BsiWI BstZ17I EcoRV FalI
FseI FspAI NruI PacI PspXI PsrI RsrII SrfI
SwaI