Restriction map for
pFN21AE6762
(Circular) (Six-base) MAPSORT of: pFN21AE6762.seq Check: 9494 from: 1 to: 6973
pFN21AE6762 6973 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 1937
Size: 2379 4594
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3077 4259 5036 6420 6688 1632
Size: 1445 1182 777 1384 268 1917
Fragments arranged by size:
1917 1445 1384 1182 777 268
AfeI AGC'GCT
Cuts at: 2034 2477 2034
Size: 443 6530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 2706 3642 5872 6849 1203
Size: 1503 936 2230 977 1327
Fragments arranged by size:
2230 1503 1327 977 936
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 3416 5702 3416
Size: 2286 4687
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3167 1184
Size: 1983 4990
AlwNI CAG_nnn'CTG
Cuts at: 3055 4716 6288 3055
Size: 1661 1572 3740
Fragments arranged by size:
3740 1661 1572
ApaI G_GGCC'C
Cuts at: 2199 3139 2199
Size: 940 6033
ApaLI G'TGCA_C
Cuts at: 5030 6186 5030
Size: 1156 5817
ApoI r'AATT_y
Cuts at: 1493 3024 4282 4508 4660 6769 1493
Size: 1531 1258 226 152 2109 1697
Fragments arranged by size:
2109 1697 1531 1258 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2526 5527 160
Size: 872 1494 3001 1606
Fragments arranged by size:
3001 1606 1494 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2093 2159 2165 2198 3113 3872
Size: 73 71 79 66 6 33 915 759
Cuts at: 3872 4298 1870
Size: 426 4545
Fragments arranged by size:
4545 915 759 426 79 73 71 66
33 6
BamHI G'GATC_C
Cuts at: 4179 4303 4670 4179
Size: 124 367 6482
Fragments arranged by size:
6482 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2217 2604 4041 4294 5749
Size: 325 217 627 430 387 1437 253 1455
Cuts at: 5749 618
Size: 1842
Fragments arranged by size:
1842 1455 1437 627 430 387 325 253
217
BanII G_rGCy'C
Cuts at: 1391 2080 2139 2160 2166 2199 2232 2348 3114
Size: 689 59 21 6 33 33 116 766
Cuts at: 3114 3139 3308 4292 1391
Size: 25 169 984 4072
Fragments arranged by size:
4072 984 766 689 169 116 59 33
33 25 21 6
BbeI G_GCGC'C
Cuts at: 2221 2608 4045 2221
Size: 387 1437 5149
Fragments arranged by size:
5149 1437 387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3614 928
Size: 971 1715 4287
Fragments arranged by size:
4287 1715 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3908 3942 5164 5198 1903
Size: 34 1971 34 1222 34 3678
Fragments arranged by size:
3678 1971 1222 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4865 6075 3588
Size: 1277 1210 4486
Fragments arranged by size:
4486 1277 1210
BclI T'GATC_A
Cuts at: 1548 2368 1548
Size: 820 6153
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2194 3051 3485
Size: 107 122 71 631 605 522 857 434
Cuts at: 3485 4231 5584 136
Size: 746 1353 1525
Fragments arranged by size:
1525 1353 857 746 631 605 522 434
122 107 71
BglII A'GATC_T
Cuts at: 2121 3075 6968 2121
Size: 954 3893 2126
Fragments arranged by size:
3893 2126 954
BlpI GC'TnA_GC
Cuts at: 4390 4390
Size: 6973
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2494 3139 3746 5034 6190 1790
Size: 409 295 645 607 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 645 607 409 295
BmgBI CAC'GTC
Cuts at: 1318 3256 1318
Size: 1938 5035
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2674 4704 5662
Size: 416 53 83 399 24 1257 2030 958
Cuts at: 5662 442
Size: 1753
Fragments arranged by size:
2030 1753 1257 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2522 2191
Size: 331 6642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 2561 2754 4077 5633 1239
Size: 907 415 193 1323 1556 2579
Fragments arranged by size:
2579 1556 1323 907 415 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2619 5098 5963 6261 2619
Size: 2479 865 298 3331
Fragments arranged by size:
3331 2479 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 3488 5636 882
Size: 617 663 1326 2148 2219
Fragments arranged by size:
2219 2148 1326 663 617
BsaAI yAC'GTr
Cuts at: 493 1204 2709 3643 3835 4764 493
Size: 711 1505 934 192 929 2702
Fragments arranged by size:
2702 1505 934 929 711 192
BsaBI GATnn'nnATC
Cuts at: 6967 6967
Size: 6973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2218 2605 3696 4042
Size: 53 83 186 936 685 387 1091 346
Cuts at: 4042 4780 5162 5797 275
Size: 738 382 635 1451
Fragments arranged by size:
1451 1091 936 738 685 635 387 382
346 186 83 53
BsaWI w'CCGG_w
Cuts at: 2290 5406 5804 6078 6225 6636 2290
Size: 3116 398 274 147 411 2627
Fragments arranged by size:
3116 2627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 4050 4080 1178
Size: 30 165 30 758 30 1859 30 4071
Fragments arranged by size:
4071 1859 758 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 3254 3718 3752 3890 1186
Size: 1033 1035 464 34 138 4269
Fragments arranged by size:
4269 1035 1033 464 138 34
BseYI C'CCAG_C
Cuts at: 2029 3154 3313 3538 6176 6672 2029
Size: 1125 159 225 2638 496 2330
Fragments arranged by size:
2638 2330 1125 496 225 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3203 1163
Size: 573 1467 4933
Fragments arranged by size:
4933 1467 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2166 2348 5034 5119 6190 2049
Size: 117 182 2686 85 1071 2832
Fragments arranged by size:
2832 2686 1071 182 117 85
BsmI GAATG_Cn'
Cuts at: 3220 4484 4577 3220
Size: 1264 93 5616
Fragments arranged by size:
5616 1264 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3236 4252 3236
Size: 1016 5957
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2080 2139 2160 2166 2199 2232
Size: 399 259 31 59 21 6 33 33
Cuts at: 2232 2348 2494 3114 3139 3308 3746 4292 5034
Size: 116 146 620 25 169 438 546 742
Cuts at: 5034 5119 6190 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 546 438 399 259
169 146 116 85 59 33 33 31
25 21 6
BspEI T'CCGG_A
Cuts at: 2290 6636 2290
Size: 4346 2627
BspHI T'CATG_A
Cuts at: 3687 4862 6592 3687
Size: 1175 1730 4068
Fragments arranged by size:
4068 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3558 3910 4328 844
Size: 2714 352 418 3489
Fragments arranged by size:
3489 2714 418 352
BsrBI CCG'CTC
Cuts at: 1394 1823 2215 4860 1394
Size: 429 392 2645 3507
Fragments arranged by size:
3507 2645 429 392
BsrDI GCAATG_nn'
Cuts at: 65 1248 2701 3151 5468 5642 65
Size: 1183 1453 450 2317 174 1396
Fragments arranged by size:
2317 1453 1396 1183 450 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5617 5804 1700
Size: 110 3807 187 2869
Fragments arranged by size:
3807 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 2147 3218 1925
Size: 222 1071 5680
Fragments arranged by size:
5680 1071 222
BssSI C'ACGA_G
Cuts at: 5033 6045 5033
Size: 1012 5961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6973
BstBI TT'CG_AA
Cuts at: 3819 5777 3819
Size: 1958 5015
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6973
BstXI CCAn_nnnn'nTGG
Cuts at: 3835 3835
Size: 6973
BstYI r'GATC_y
Cuts at: 1972 2121 2729 2769 2871 2893 3075 4179 4303
Size: 149 608 40 102 22 182 1104 124
Cuts at: 4303 4670 5055 5072 6524 6610 6622 6968 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1104 608 385 367 346 182
149 124 102 86 40 22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2466 2799 3139 3480 513
Size: 552 1401 333 340 341 4006
Fragments arranged by size:
4006 1401 552 341 340 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3112 487
Size: 683 56 1886 4348
Fragments arranged by size:
4348 1886 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2588 2885 4092 4489 5283 5303 2061
Size: 527 297 1207 397 794 20 3731
Fragments arranged by size:
3731 1207 794 527 397 297 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6938
DraI TTT'AAA
Cuts at: 3381 4277 3381
Size: 896 6077
DraIII CAC_nnn'GTG
Cuts at: 3235 4143 3235
Size: 908 6065
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 4173 4420
Size: 54 1425 50 275 18 234 2109 247
Cuts at: 4420 5309 6938 8
Size: 889 1629 43
Fragments arranged by size:
2109 1629 1425 889 275 247 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4250 4903 1353
Size: 2897 653 3423
Fragments arranged by size:
3423 2897 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2169 4267 5560 5934 6080 387
Size: 1064 138 580 2098 1293 374 146 1280
Fragments arranged by size:
2098 1293 1280 1064 580 374 146 138
EcoICRI GAG'CTC
Cuts at: 2164 2346 2164
Size: 182 6791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2561 2754 3077 4077 4259 5036
Size: 393 514 415 193 323 1000 182 777
Cuts at: 5036 5633 6420 6688 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1000 787 777 597 514 415 393
323 268 193 182
EcoNI CCTnn'n_nnAGG
Cuts at: 2304 3236 2304
Size: 932 6041
EcoO109I rG'GnC_Cy
Cuts at: 2098 2195 3052 3135 3756 2098
Size: 97 857 83 621 5315
Fragments arranged by size:
5315 857 621 97 83
EcoRI G'AATT_C
Cuts at: 4282 4660 4282
Size: 378 6595
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 2036 2221 2479 2608 4045 6120 1678
Size: 358 185 258 129 1437 2075 2531
Fragments arranged by size:
2531 2075 1437 358 258 185 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2496 2528 3067
Size: 32 135 32 589 32 303 32 539
Cuts at: 3067 3099 3402 3434 5614 5646 5688 5720 1373
Size: 32 303 32 2180 32 42 32 2626
Fragments arranged by size:
2626 2180 589 539 303 303 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4317 4563 669
Size: 988 281 2379 246 3079
Fragments arranged by size:
3079 2379 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6973
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KasI G'GCGC_C
Cuts at: 2217 2604 4041 2217
Size: 387 1437 5149
Fragments arranged by size:
5149 1437 387
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 4572 4572
Size: 6973
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 3450 6087 6271 1305
Size: 748 1397 2637 184 2007
Fragments arranged by size:
2637 2007 1397 748 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4175 10
Size: 54 1425 343 2343 2808
Fragments arranged by size:
2808 2343 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 2864 3144 3833 4931 5290 5449
Size: 648 1692 6 280 689 1098 359 159
Cuts at: 5449 518
Size: 2042
Fragments arranged by size:
2042 1692 1098 689 648 359 280 159
6
MspA1I CmG'CkG
Cuts at: 1606 2185 3651 3868 4389 5066 6214 6459 1606
Size: 579 1466 217 521 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 579 521 245 217
NarI GG'CG_CC
Cuts at: 2218 2605 4042 2218
Size: 387 1437 5149
Fragments arranged by size:
5149 1437 387
NcoI C'CATG_G
Cuts at: 513 1065 2799 513
Size: 552 1734 4687
Fragments arranged by size:
4687 1734 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 4331 5876 1132
Size: 97 3102 1545 2229
Fragments arranged by size:
3102 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2080 3089 3328 1348
Size: 732 1009 239 4993
Fragments arranged by size:
4993 1009 732 239
PciI A'CATG_T
Cuts at: 5872 5872
Size: 6973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2420 3235 1108
Size: 1312 815 4846
Fragments arranged by size:
4846 1312 815
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PmlI CAC'GTG
Cuts at: 2709 3643 2709
Size: 934 6039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 5042 5074 6555 6587 3101
Size: 32 1909 32 1481 32 3487
Fragments arranged by size:
3487 1909 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2098
Size: 6973
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6973
PsiI TTA'TAA
Cuts at: 2967 4543 2967
Size: 1576 5397
PspOMI G'GGCC_C
Cuts at: 2195 3135 2195
Size: 940 6033
PspXI vC'TCGA_Gb
Cuts at: 2093 2093
Size: 6973
PstI C_TGCA'G
Cuts at: 830 3555 4325 830
Size: 2725 770 3478
Fragments arranged by size:
3478 2725 770
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 3868 3868
Size: 6973
SacI G_AGCT'C
Cuts at: 2166 2348 2166
Size: 182 6791
SalI G'TCGA_C
Cuts at: 1936 4315 1936
Size: 2379 4594
SbfI CC_TGCA'GG
Cuts at: 3555 4325 3555
Size: 770 6203
ScaI AGT'ACT
Cuts at: 3203 5221 6933 3203
Size: 2018 1712 3243
Fragments arranged by size:
3243 2018 1712
SexAI A'CCwGG_T
Cuts at: 2305 3231 3588 2305
Size: 926 357 5690
Fragments arranged by size:
5690 926 357
SfcI C'TryA_G
Cuts at: 826 1045 3551 4321 5456 6137 6328 826
Size: 219 2506 770 1135 681 191 1471
Fragments arranged by size:
2506 1471 1135 770 681 219 191
SfoI GGC'GCC
Cuts at: 2219 2606 4043 2219
Size: 387 1437 5149
Fragments arranged by size:
5149 1437 387
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 2016 2200 4300 1872
Size: 144 184 2100 4545
Fragments arranged by size:
4545 2100 184 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 2165 2634 5077 5978 6240
Size: 197 926 150 72 469 2443 901 262
Cuts at: 6240 820
Size: 1553
Fragments arranged by size:
2443 1553 926 901 469 262 197 150
72
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6973
SphI G_CATG'C
Cuts at: 1132 4331 1132
Size: 3199 3774
SspI AAT'ATT
Cuts at: 5 52 4897 5
Size: 47 4845 2081
Fragments arranged by size:
4845 2081 47
StuI AGG'CCT
Cuts at: 1517 3293 1517
Size: 1776 5197
StyI C'CwwG_G
Cuts at: 513 1065 2799 4412 513
Size: 552 1734 1613 3074
Fragments arranged by size:
3074 1734 1613 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2656 5000 5017 5170 5355 5800 325
Size: 1255 1076 2344 17 153 185 445 1498
Fragments arranged by size:
2344 1498 1255 1076 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3201 5219 6931
Size: 274 80 33 51 1443 1224 2018 1712
Cuts at: 6931 96
Size: 138
Fragments arranged by size:
2018 1712 1443 1224 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4050 4082 6933 6965 4050
Size: 32 2851 32 4058
Fragments arranged by size:
4058 2851 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6973
XbaI T'CTAG_A
Cuts at: 4309 4309
Size: 6973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2941 4111 2941
Size: 1170 5803
XhoI C'TCGA_G
Cuts at: 1943 2093 2165 1943
Size: 150 72 6751
Fragments arranged by size:
6751 150 72
XmaI C'CCGG_G
Cuts at: 1870 2014 2198 4298 1870
Size: 144 184 2100 4545
Fragments arranged by size:
4545 2100 184 144
XmnI GAAnn'nnTTC
Cuts at: 3791 5102 3791
Size: 1311 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI