Restriction map for
pFN21AE6760
(Circular) (Six-base) MAPSORT of: pFN21AE6760.seq Check: 6253 from: 1 to: 6301
pFN21AE6760 6301 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2853 3074 3418 4364 5748 6016 1632
Size: 881 340 221 344 946 1384 268 1917
Fragments arranged by size:
1917 1384 946 881 344 340 268 221
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 3043 3163 5200 6177 1203
Size: 1840 120 2037 977 1327
Fragments arranged by size:
2037 1840 1327 977 120
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 4987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2671 2717 1184
Size: 1487 46 4768
Fragments arranged by size:
4768 1487 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3021 3053 3021
Size: 32 6269
AlwNI CAG_nnn'CTG
Cuts at: 2870 4044 5616 2870
Size: 1174 1572 3555
Fragments arranged by size:
3555 1572 1174
ApaLI G'TGCA_C
Cuts at: 2806 4358 5514 2806
Size: 1552 1156 3593
Fragments arranged by size:
3593 1552 1156
ApoI r'AATT_y
Cuts at: 1493 2921 3131 3610 3836 3988 6097 1493
Size: 1428 210 479 226 152 2109 1697
Fragments arranged by size:
2109 1697 1428 479 226 210 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2504 3195 3376 3622
Size: 325 217 627 221 496 691 181 246
Cuts at: 3622 5077 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 691 627 496 325 246 221
217 181
BanII G_rGCy'C
Cuts at: 1391 2678 2791 3620 1391
Size: 1287 113 829 4072
Fragments arranged by size:
4072 1287 829 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2841 3424 928
Size: 971 942 583 3805
Fragments arranged by size:
3805 971 942 583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 2256 3186 3529 1548
Size: 708 930 343 4320
Fragments arranged by size:
4320 930 708 343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2568 2977 6296 2568
Size: 409 3319 2573
Fragments arranged by size:
3319 2573 409
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 2810 3442 4362 5518 1790
Size: 1020 632 920 1156 2573
Fragments arranged by size:
2573 1156 1020 920 632
BmgBI CAC'GTC
Cuts at: 1318 2245 1318
Size: 927 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3479 3547 4032
Size: 416 53 83 399 24 2062 68 485
Cuts at: 4032 4990 442
Size: 958 1753
Fragments arranged by size:
2062 1753 958 485 416 399 83 68
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2727 4961 1239
Size: 1488 2234 2579
Fragments arranged by size:
2579 2234 1488
Bpu10I CC'TnA_GC
Cuts at: 3381 3381
Size: 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4426 5291 5589 4426
Size: 865 298 5138
Fragments arranged by size:
5138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4964 882
Size: 617 3465 2219
Fragments arranged by size:
3465 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3021 3051 1178
Size: 30 165 30 1618 30 4428
Fragments arranged by size:
4428 1618 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6301
BseYI C'CCAG_C
Cuts at: 3579 5504 6000 3579
Size: 1925 496 3880
Fragments arranged by size:
3880 1925 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2623 1163
Size: 573 887 4841
Fragments arranged by size:
4841 887 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2132 3751 4512 5540 1540
Size: 275 186 131 1619 761 1028 2301
Fragments arranged by size:
2301 1619 1028 761 275 186 131
BsiHKAI G_wGCw'C
Cuts at: 2810 4362 4447 5518 2810
Size: 1552 85 1071 3593
Fragments arranged by size:
3593 1552 1071 85
BsmI GAATG_Cn'
Cuts at: 2028 2424 2694 2706 3484 3812 3905 2028
Size: 396 270 12 778 328 93 4424
Fragments arranged by size:
4424 778 396 328 270 93 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2678 2791 2810 3442 3620 4362 4447
Size: 399 888 113 19 632 178 742 85
Cuts at: 4447 5518 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 888 742 632 399 178 113
85 19
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 2439 4190 5920 2439
Size: 1751 1730 2820
Fragments arranged by size:
2820 1751 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 844
Size: 2812 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2144 2457 3096 3274 4796 4970 65
Size: 1183 896 313 639 178 1522 174 1396
Fragments arranged by size:
1522 1396 1183 896 639 313 178 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2131 4945 5132 1700
Size: 110 321 2814 187 2869
Fragments arranged by size:
2869 2814 321 187 110
BsrGI T'GTAC_A
Cuts at: 96 3213 96
Size: 3117 3184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 2698 5105 2698
Size: 2407 3894
BstEII G'GTnAC_C
Cuts at: 1184 3149 1184
Size: 1965 4336
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 3235 2482
Size: 753 5548
BstYI r'GATC_y
Cuts at: 1972 2568 2977 3631 3998 4383 4400 5852 5938
Size: 596 409 654 367 385 17 1452 86
Cuts at: 5938 5950 6296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 654 596 409 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3154 513
Size: 552 939 1150 3660
Fragments arranged by size:
3660 1150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2306 487
Size: 683 56 1080 4482
Fragments arranged by size:
4482 1080 683 56
BtsI GCAGTG_nn'
Cuts at: 3300 3817 4611 4631 3300
Size: 517 794 20 4970
Fragments arranged by size:
4970 794 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 2910 3605 2910
Size: 695 5606
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 2172 2575
Size: 54 1425 50 275 18 299 43 403
Cuts at: 2575 3748 4637 6266 8
Size: 1173 889 1629 43
Fragments arranged by size:
1629 1425 1173 889 403 299 275 54
50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2129 3748 1537
Size: 275 317 1619 4090
Fragments arranged by size:
4090 1619 317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4231 1353
Size: 2878 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2727 2853 3074 3418 4364 4961
Size: 393 881 214 126 221 344 946 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 946 881 787 597 393 344 268
221 214 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2742 2742
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2867 2867
Size: 6301
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2054 5448 1678
Size: 376 3394 2531
Fragments arranged by size:
3394 2531 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2859 2891 2933 2965 4942
Size: 32 135 32 1287 32 42 32 1977
Cuts at: 4942 4974 5016 5048 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1977 1287 135 42 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2321 2400 748
Size: 1573 79 4649
Fragments arranged by size:
4649 1573 79
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 3225 5415 5599 1305
Size: 904 1016 2190 184 2007
Fragments arranged by size:
2190 2007 1016 904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 10
Size: 54 1425 343 342 4137
Fragments arranged by size:
4137 1425 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 2699 4259 4618 4777 518
Size: 648 1314 219 1560 359 159 2042
Fragments arranged by size:
2042 1560 1314 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2651 3717 4394 5542 5787 1606
Size: 564 481 1066 677 1148 245 2120
Fragments arranged by size:
2120 1148 1066 677 564 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3154 513
Size: 552 939 1150 3660
Fragments arranged by size:
3660 1150 939 552
NdeI CA'TA_TG
Cuts at: 387 2593 387
Size: 2206 4095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 3047 3167 3659 5204 1132
Size: 97 1818 120 492 1545 2229
Fragments arranged by size:
2229 1818 1545 492 120 97
PasI CC'CwG_GG
Cuts at: 1348 3442 1348
Size: 2094 4207
PciI A'CATG_T
Cuts at: 3043 3163 5200 3043
Size: 120 2037 4144
Fragments arranged by size:
4144 2037 120
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3511 3559 1108
Size: 2403 48 3850
Fragments arranged by size:
3850 2403 48
PfoI T'CCnGG_A
Cuts at: 3589 3589
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3021 3053 4370 4402 5883 5915 3021
Size: 32 1317 32 1481 32 3407
Fragments arranged by size:
3407 1481 1317 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2867 2867
Size: 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2170 2651 2170
Size: 481 5820
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 3068 3557 4549 6261 3068
Size: 489 992 1712 3108
Fragments arranged by size:
3108 1712 992 489
SfcI C'TryA_G
Cuts at: 826 1045 2310 2530 3649 4784 5465 5656 826
Size: 219 1265 220 1119 1135 681 191 1471
Fragments arranged by size:
1471 1265 1135 1119 681 220 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4405 5306 5568 820
Size: 197 926 2462 901 262 1553
Fragments arranged by size:
2462 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 2004 2374 2775 3154 3740 513
Size: 552 939 370 401 379 586 3074
Fragments arranged by size:
3074 939 586 552 401 379 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 3066 3213
Size: 274 80 33 51 1443 297 792 147
Cuts at: 3213 3555 4547 6259 96
Size: 342 992 1712 138
Fragments arranged by size:
1712 1443 992 792 342 297 274 147
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3512 3512
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 3021 4430 3021
Size: 1409 4892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI