Restriction map for
pFN21AE6759
(Circular) (Six-base) MAPSORT of: pFN21AE6759.seq Check: 8514 from: 1 to: 6334
pFN21AE6759 6334 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2791 4397 5781 6049 1632
Size: 1159 1606 1384 268 1917
Fragments arranged by size:
1917 1606 1384 1159 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 3007 5063 3007
Size: 2056 4278
AleI CACnn'nnGTG
Cuts at: 1166 2934 1166
Size: 1768 4566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2119 2323 3028 4077 5649 2119
Size: 204 705 1049 1572 2804
Fragments arranged by size:
2804 1572 1049 705 204
ApaI G_GGCC'C
Cuts at: 2165 2165
Size: 6334
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 2783 3514 3607 3643 3869 4021 6130 1493
Size: 1290 731 93 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 1290 731 226 152 93 36
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2501 2581 2660 2858 3066 3659
Size: 73 215 343 80 79 198 208 593
Cuts at: 3659 1870
Size: 4545
Fragments arranged by size:
4545 593 343 215 208 198 80 79
73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 2990 3655 5110 618
Size: 325 217 627 780 423 665 1455 1842
Fragments arranged by size:
1842 1455 780 665 627 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2339 3588 3653 1391
Size: 774 174 1249 65 4072
Fragments arranged by size:
4072 1249 774 174 65
BbeI G_GCGC'C
Cuts at: 2571 2994 2571
Size: 423 5911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5363
BbvCI CC'TCA_GC
Cuts at: 2018 2123 2018
Size: 105 6229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2386 2420 4525 4559 1903
Size: 34 449 34 2105 34 3678
Fragments arranged by size:
3678 2105 449 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3060 4226 5436 3060
Size: 1166 1210 3958
Fragments arranged by size:
3958 1210 1166
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 2516 2678 3751 2516
Size: 162 1073 5099
Fragments arranged by size:
5099 1073 162
Bme1580I G_kGCm'C
Cuts at: 1790 2165 4395 5551 1790
Size: 375 2230 1156 2573
Fragments arranged by size:
2573 2230 1156 375
BmgBI CAC'GTC
Cuts at: 1318 2731 1318
Size: 1413 4921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3290 4065 5023
Size: 416 53 83 399 24 1873 775 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1873 1753 958 775 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3163 4994 1239
Size: 1924 1831 2579
Fragments arranged by size:
2579 1924 1831
Bpu10I CC'TnA_GC
Cuts at: 2018 2123 2018
Size: 105 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2854 4459 5324 5622 2854
Size: 1605 865 298 3566
Fragments arranged by size:
3566 1605 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2512 4125 493
Size: 711 1308 1613 2702
Fragments arranged by size:
2702 1613 1308 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 2886 2991 4141
Size: 53 83 186 936 1035 318 105 1150
Cuts at: 4141 4523 5158 275
Size: 382 635 1451
Fragments arranged by size:
1451 1150 1035 936 635 382 318 186
105 83 53
BsaWI w'CCGG_w
Cuts at: 2145 4767 5165 5439 5586 5997 2145
Size: 2622 398 274 147 411 2482
Fragments arranged by size:
2622 2482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3091 3121 1178
Size: 30 165 30 1688 30 4391
Fragments arranged by size:
4391 1688 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3023 3026 1186
Size: 1837 3 4494
Fragments arranged by size:
4494 1837 3
BseYI C'CCAG_C
Cuts at: 5537 6033 5537
Size: 496 5838
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2453 3080 3092 1163
Size: 573 612 105 627 12 4405
Fragments arranged by size:
4405 627 612 573 105 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2353 2357 2392 2919 3784 4545
Size: 275 186 352 4 35 527 865 761
Cuts at: 4545 5573 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 865 761 527 352 275 186
35 4
BsiHKAI G_wGCw'C
Cuts at: 2424 3588 4395 4480 5551 2424
Size: 1164 807 85 1071 3207
Fragments arranged by size:
3207 1164 1071 807 85
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2339 2424 3588 3653 4395 4480
Size: 399 375 174 85 1164 65 742 85
Cuts at: 4480 5551 1391
Size: 1071 2174
Fragments arranged by size:
2174 1164 1071 742 399 375 174 85
85 65
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2616 3289 4223 5953 2616
Size: 673 934 1730 2997
Fragments arranged by size:
2997 1730 934 673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3144 3689 844
Size: 2300 545 3489
Fragments arranged by size:
3489 2300 545
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2352 2356 2965 4978 5165 1700
Size: 110 542 4 609 2013 187 2869
Fragments arranged by size:
2869 2013 609 542 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 2305 2440 2525 2940 1925
Size: 380 135 85 415 5319
Fragments arranged by size:
5319 415 380 135 85
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3028 794
Size: 2234 4100
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 3122 1184
Size: 1938 4396
BstXI CCAn_nnnn'nTGG
Cuts at: 3303 3303
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2262 3664 4031 4416 4433 5885 5971 5983
Size: 290 1402 367 385 17 1452 86 12
Cuts at: 5983 6329 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1402 385 367 346 290 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2604 3579 513
Size: 552 939 600 975 3268
Fragments arranged by size:
3268 975 939 600 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3029 3213 3850 4644 4664 2839
Size: 190 184 637 794 20 4509
Fragments arranged by size:
4509 794 637 190 184 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3553 3638 3553
Size: 85 6249
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2094 2174
Size: 54 1425 50 275 18 206 58 80
Cuts at: 2174 2354 2389 2407 2601 2607 2916 2967 3781
Size: 180 35 18 194 6 309 51 814
Cuts at: 3781 4670 6299 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 814 309 275 206 194
180 80 58 54 51 50 43 35
18 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2354 2389 2916 3781 1537
Size: 275 542 35 527 865 4090
Fragments arranged by size:
4090 865 542 527 275 35
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 3044 3455 4264 1353
Size: 1332 359 411 809 3423
Fragments arranged by size:
3423 1332 809 411 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 4921 5295 5441 387
Size: 1064 138 666 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 666 374 146 138
EcoICRI GAG'CTC
Cuts at: 3586 3586
Size: 6334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2791 3163 4397 4994 5781 6049 1239
Size: 393 1159 372 1234 597 787 268 1524
Fragments arranged by size:
1524 1234 1159 787 597 393 372 268
EcoO109I rG'GnC_Cy
Cuts at: 2320 3367 2320
Size: 1047 5287
EcoRI G'AATT_C
Cuts at: 2783 3643 4021 2783
Size: 860 378 5096
Fragments arranged by size:
5096 860 378
FseI GG_CCGG'CC
Cuts at: 2360 2360
Size: 6334
FspI TGC'GCA
Cuts at: 2462 2768 4840 2462
Size: 306 2072 3956
Fragments arranged by size:
3956 2072 306
FspAI rTGC'GCAy
Cuts at: 2768 2768
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2245 2543 2571 2994 5481 1678
Size: 567 298 28 423 2487 2531
Fragments arranged by size:
2531 2487 567 423 298 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2993 3025 3091 3123 4975
Size: 32 135 32 1421 32 66 32 1852
Cuts at: 4975 5007 5049 5081 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1852 1421 135 66 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3271 3678 3924 669
Size: 988 281 1333 407 246 3079
Fragments arranged by size:
3079 1333 988 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2567 2990 2567
Size: 423 5911
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2176 2609 10
Size: 54 1425 343 264 80 433 3735
Fragments arranged by size:
3735 1425 433 343 264 80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2934 4292 4651 4810 518
Size: 648 1122 646 1358 359 159 2042
Fragments arranged by size:
2042 1358 1122 648 646 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2116 2277 2437 2590 3750 4427 5575
Size: 444 66 161 160 153 1160 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1160 1148 677 444 245 161 160
153 66
NaeI GCC'GGC
Cuts at: 2358 2358
Size: 6334
NarI GG'CG_CC
Cuts at: 2568 2991 2568
Size: 423 5911
NcoI C'CATG_G
Cuts at: 513 1065 2004 3579 513
Size: 552 939 1575 3268
Fragments arranged by size:
3268 1575 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 2356 2356
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 2389 3781 2389
Size: 1392 4942
NspI r_CATG'y
Cuts at: 1132 1229 2108 3692 5237 1132
Size: 97 879 1584 1545 2229
Fragments arranged by size:
2229 1584 1545 879 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 5119
PfoI T'CCnGG_A
Cuts at: 2820 2820
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2512 2512
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3367 3367
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspOMI G'GGCC_C
Cuts at: 2161 2161
Size: 6334
PspXI vC'TCGA_Gb
Cuts at: 2501 3066 2501
Size: 565 5769
PstI C_TGCA'G
Cuts at: 830 2748 3024 3686 830
Size: 1918 276 662 3478
Fragments arranged by size:
3478 1918 662 276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2277 2277
Size: 6334
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 6334
SacI G_AGCT'C
Cuts at: 3588 3588
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 2750 4582 6294 2750
Size: 1832 1712 2790
Fragments arranged by size:
2790 1832 1712
SexAI A'CCwGG_T
Cuts at: 3044 3044
Size: 6334
SfcI C'TryA_G
Cuts at: 826 1045 2644 2744 3020 3682 4817 5498 5689
Size: 219 1599 100 276 662 1135 681 191
Cuts at: 5689 826
Size: 1471
Fragments arranged by size:
1599 1471 1135 681 662 276 219 191
100
SfoI GGC'GCC
Cuts at: 2569 2992 2569
Size: 423 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 2160 2860 3661 1872
Size: 288 700 801 4545
Fragments arranged by size:
4545 801 700 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2833 3066 4438 5339 5601
Size: 197 926 558 332 233 1372 901 262
Cuts at: 5601 820
Size: 1553
Fragments arranged by size:
1553 1372 926 901 558 332 262 233
197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2033 3249 3579 3618 3773 513
Size: 552 939 29 1216 330 39 155 3074
Fragments arranged by size:
3074 1216 939 552 330 155 39 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3110 4361 4378 4531 4716 5161 325
Size: 1255 1530 1251 17 153 185 445 1498
Fragments arranged by size:
1530 1498 1255 1251 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 4580 6292
Size: 274 80 33 51 1443 771 1832 1712
Cuts at: 6292 96
Size: 138
Fragments arranged by size:
1832 1712 1443 771 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3034 3066 6294 6326 3034
Size: 32 3228 32 3042
Fragments arranged by size:
3228 3042 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2104 3256 2104
Size: 1152 5182
XhoI C'TCGA_G
Cuts at: 1943 2501 3066 1943
Size: 558 565 5211
Fragments arranged by size:
5211 565 558
XmaI C'CCGG_G
Cuts at: 1870 2158 2858 3659 1870
Size: 288 700 801 4545
Fragments arranged by size:
4545 801 700 288
XmnI GAAnn'nnTTC
Cuts at: 2850 4463 2850
Size: 1613 4721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
NruI NsiI PacI PsrI SacII SanDI SapI SfiI
SrfI SwaI