Restriction map for
pFN21AE6758
(Circular) (Six-base) MAPSORT of: pFN21AE6758.seq Check: 4157 from: 1 to: 6454
pFN21AE6758 6454 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2525 3261 3797 1937
Size: 588 736 536 4594
Fragments arranged by size:
4594 736 588 536
Acc65I G'GTAC_C
Cuts at: 3775 3775
Size: 6454
AclI AA'CG_TT
Cuts at: 1563 4581 4954 1563
Size: 3018 373 3063
Fragments arranged by size:
3063 3018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2454 3017 3097 3288 3692 4517 5901
Size: 470 352 563 80 191 404 825 1384
Cuts at: 5901 6169 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 825 563 470 404 352 268
191 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6257
AflIII A'CryG_T
Cuts at: 1203 2306 5353 6330 1203
Size: 1103 3047 977 1327
Fragments arranged by size:
3047 1327 1103 977
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6454
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 6422
AlwNI CAG_nnn'CTG
Cuts at: 3508 3571 4197 5769 3508
Size: 63 626 1572 4193
Fragments arranged by size:
4193 1572 626 63
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2279 2569 3616 3763 3989 4141 6250 1493
Size: 786 290 1047 147 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 786 290 226 152 147
AseI AT'TA_AT
Cuts at: 160 1032 5008 160
Size: 872 3976 1606
Fragments arranged by size:
3976 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 1870
Size: 73 1836 4545
Fragments arranged by size:
4545 1836 73
AvrII C'CTAG_G
Cuts at: 2812 2812
Size: 6454
BamHI G'GATC_C
Cuts at: 2773 3784 4151 2773
Size: 1011 367 5076
Fragments arranged by size:
5076 1011 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 3775 5230 618
Size: 325 217 627 1592 396 1455 1842
Fragments arranged by size:
1842 1592 1455 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2339 3773 1391
Size: 948 1434 4072
Fragments arranged by size:
4072 1434 948
BbeI G_GCGC'C
Cuts at: 3383 3383
Size: 6454
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2692 928
Size: 971 793 4690
Fragments arranged by size:
4690 971 793
BbvCI CC'TCA_GC
Cuts at: 3375 3375
Size: 6454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3457 3491 4645 4679 1903
Size: 34 1520 34 1154 34 3678
Fragments arranged by size:
3678 1520 1154 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3262 4346 5556 3262
Size: 1084 1210 4160
Fragments arranged by size:
4160 1210 1084
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5065 136
Size: 107 122 71 631 605 3393 1525
Fragments arranged by size:
3393 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6449 6449
Size: 6454
BlpI GC'TnA_GC
Cuts at: 2252 3871 2252
Size: 1619 4835
Bme1580I G_kGCm'C
Cuts at: 1790 4515 5671 1790
Size: 2725 1156 2573
Fragments arranged by size:
2725 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2351 2929 3445
Size: 416 53 83 399 24 934 578 516
Cuts at: 3445 4185 5143 442
Size: 740 958 1753
Fragments arranged by size:
1753 958 934 740 578 516 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 3140 3446 3462 3508 5114 1239
Size: 921 980 306 16 46 1606 2579
Fragments arranged by size:
2579 1606 980 921 306 46 16
Bpu10I CC'TnA_GC
Cuts at: 3375 3375
Size: 6454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4579 5444 5742 4579
Size: 865 298 5291
Fragments arranged by size:
5291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5117 882
Size: 617 3618 2219
Fragments arranged by size:
3618 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4245 493
Size: 711 3041 2702
Fragments arranged by size:
3041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3380 4261 4643 5278
Size: 53 83 186 936 1847 881 382 635
Cuts at: 5278 275
Size: 1451
Fragments arranged by size:
1847 1451 936 881 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 3113 3143 1178
Size: 30 165 30 933 30 747 30 4489
Fragments arranged by size:
4489 933 747 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2122 2476 2899 2942 3505 1186
Size: 886 50 354 423 43 563 4135
Fragments arranged by size:
4135 886 563 423 354 50 43
BseYI C'CCAG_C
Cuts at: 2894 5657 6153 2894
Size: 2763 496 3195
Fragments arranged by size:
3195 2763 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2487 3133 3341 3558 1163
Size: 573 751 646 208 217 4059
Fragments arranged by size:
4059 751 646 573 217 208
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2784 2821 3160 4515 4600 5671 2784
Size: 37 339 1355 85 1071 3567
Fragments arranged by size:
3567 1355 1071 339 85 37
BsmI GAATG_Cn'
Cuts at: 2707 2750 3356 3553 3965 4058 2707
Size: 43 606 197 412 93 5103
Fragments arranged by size:
5103 606 412 197 93 43
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 2784 2821 3160 3773 4515 4600
Size: 399 549 445 37 339 613 742 85
Cuts at: 4600 5671 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 613 549 445 399 339
85 37
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 4343 6073 4343
Size: 1730 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2537 3809 844
Size: 1693 1272 3489
Fragments arranged by size:
3489 1693 1272
BsrBI CCG'CTC
Cuts at: 1394 1823 4341 1394
Size: 429 2518 3507
Fragments arranged by size:
3507 2518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4949 5123 65
Size: 1183 3701 174 1396
Fragments arranged by size:
3701 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2643 5098 5285 1700
Size: 110 833 2455 187 2869
Fragments arranged by size:
2869 2455 833 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2863 794
Size: 2069 4385
BstBI TT'CG_AA
Cuts at: 5258 5258
Size: 6454
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 4925
BstYI r'GATC_y
Cuts at: 1972 2427 2494 2773 3283 3784 4151 4536 4553
Size: 455 67 279 510 501 367 385 17
Cuts at: 4553 6005 6091 6103 6449 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 510 501 455 385 367 346
279 86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2340 2340
Size: 6454
BtgI C'CryG_G
Cuts at: 513 1065 2004 2829 3246 3643 513
Size: 552 939 825 417 397 3324
Fragments arranged by size:
3324 939 825 552 417 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5715
Fragments arranged by size:
5715 683 56
BtsI GCAGTG_nn'
Cuts at: 2263 2819 2878 3596 3970 4764 4784 2263
Size: 556 59 718 374 794 20 3933
Fragments arranged by size:
3933 794 718 556 374 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3351 3386 529
Size: 35 2787 35 3597
Fragments arranged by size:
3597 2787 35 35
DraI TTT'AAA
Cuts at: 3758 3758
Size: 6454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 809
Size: 507 4145 1802
Fragments arranged by size:
4145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 3339 3531
Size: 54 1425 50 275 18 706 803 192
Cuts at: 3531 3901 4790 6419 8
Size: 370 889 1629 43
Fragments arranged by size:
1629 1425 889 803 706 370 275 192
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2620 3169 3264 4384 1353
Size: 768 499 549 95 1120 3423
Fragments arranged by size:
3423 1120 768 549 499 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5041 5415 5561 387
Size: 1064 138 3452 374 146 1280
Fragments arranged by size:
3452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2160 2454 3017 3097 3140 3288
Size: 393 470 58 294 563 80 43 148
Cuts at: 3288 3446 3462 3508 3692 4517 5114 5901 6169
Size: 158 16 46 184 825 597 787 268
Cuts at: 6169 1239
Size: 1524
Fragments arranged by size:
1524 825 787 597 563 470 393 294
268 184 158 148 80 58 46 43
16
EcoNI CCTnn'n_nnAGG
Cuts at: 3575 3575
Size: 6454
EcoO109I rG'GnC_Cy
Cuts at: 2482 2482
Size: 6454
EcoRI G'AATT_C
Cuts at: 3763 4141 3763
Size: 378 6076
FspI TGC'GCA
Cuts at: 3355 4960 3355
Size: 1605 4849
FspAI rTGC'GCAy
Cuts at: 3355 3355
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 3383 5601 1678
Size: 1705 2218 2531
Fragments arranged by size:
2531 2218 1705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2336 2368 2479
Size: 32 135 32 464 32 268 32 111
Cuts at: 2479 2511 3082 3114 5095 5127 5169 5201 1373
Size: 32 571 32 1981 32 42 32 2626
Fragments arranged by size:
2626 1981 571 464 268 135 111 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 3798 4044 669
Size: 988 281 208 1652 246 3079
Fragments arranged by size:
3079 1652 988 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6454
HpaI GTT'AAC
Cuts at: 4044 4044
Size: 6454
KasI G'GCGC_C
Cuts at: 3379 3379
Size: 6454
KpnI G_GTAC'C
Cuts at: 3779 3779
Size: 6454
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5568 5752 1305
Size: 4263 184 2007
Fragments arranged by size:
4263 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2538 3533 10
Size: 54 1425 343 706 995 2931
Fragments arranged by size:
2931 1425 995 706 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2638 2765 3635 4412 4771 4930 518
Size: 648 1472 127 870 777 359 159 2042
Fragments arranged by size:
2042 1472 870 777 648 359 159 127
MspA1I CmG'CkG
Cuts at: 1606 2841 3508 3870 4547 5695 5940 1606
Size: 1235 667 362 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 667 362 245
NaeI GCC'GGC
Cuts at: 2645 2645
Size: 6454
NarI GG'CG_CC
Cuts at: 3380 3380
Size: 6454
NcoI C'CATG_G
Cuts at: 513 1065 2004 2829 3246 513
Size: 552 939 825 417 3721
Fragments arranged by size:
3721 939 825 552 417
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6454
NgoMIV G'CCGG_C
Cuts at: 2643 2643
Size: 6454
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6454
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NspI r_CATG'y
Cuts at: 1132 1229 2310 3354 3812 5357 1132
Size: 97 1081 1044 458 1545 2229
Fragments arranged by size:
2229 1545 1081 1044 458 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6454
PciI A'CATG_T
Cuts at: 2306 5353 2306
Size: 3047 3407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6454
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4523 4555 6036 6068 3113
Size: 32 1378 32 1481 32 3499
Fragments arranged by size:
3499 1481 1378 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 2482
Size: 6454
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6454
PsiI TTA'TAA
Cuts at: 4024 4024
Size: 6454
PstI C_TGCA'G
Cuts at: 830 2826 3291 3806 830
Size: 1996 465 515 3478
Fragments arranged by size:
3478 1996 515 465
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
PvuII CAG'CTG
Cuts at: 3508 3508
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 2940 4702 6414 2940
Size: 1762 1712 2980
Fragments arranged by size:
2980 1762 1712
SfcI C'TryA_G
Cuts at: 826 1045 2822 3287 3802 4937 5618 5809 826
Size: 219 1777 465 515 1135 681 191 1471
Fragments arranged by size:
1777 1471 1135 681 515 465 219 191
SfoI GGC'GCC
Cuts at: 3381 3381
Size: 6454
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4558 5459 5721 820
Size: 197 926 2615 901 262 1553
Fragments arranged by size:
2615 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 3481 152
Size: 3329 3125
SphI G_CATG'C
Cuts at: 1132 3812 1132
Size: 2680 3774
SspI AAT'ATT
Cuts at: 5 52 4378 5
Size: 47 4326 2081
Fragments arranged by size:
4326 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6454
StyI C'CwwG_G
Cuts at: 513 1065 2004 2812 2829 3246 3893 513
Size: 552 939 808 17 417 647 3074
Fragments arranged by size:
3074 939 808 647 552 417 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2911 4481 4498 4651 4836 5281 325
Size: 1255 1331 1570 17 153 185 445 1498
Fragments arranged by size:
1570 1498 1331 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2938 4700 6412
Size: 274 80 33 51 1443 961 1762 1712
Cuts at: 6412 96
Size: 138
Fragments arranged by size:
1762 1712 1443 961 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 6414 6446 2187
Size: 32 4195 32 2195
Fragments arranged by size:
4195 2195 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6454
XbaI T'CTAG_A
Cuts at: 3790 3790
Size: 6454
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6454
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 3030 3110 4583 2283
Size: 747 80 1473 4154
Fragments arranged by size:
4154 1473 747 80
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI