Restriction map for
pFN21AE6756
(Circular) (Six-base) MAPSORT of: pFN21AE6756.seq Check: 1753 from: 1 to: 7141
pFN21AE6756 7141 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4951 278
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4484 1937
Size: 2547 4594
Acc65I G'GTAC_C
Cuts at: 4462 4462
Size: 7141
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2712 3324 3365 4203 5204 6588 6856 1632
Size: 1080 612 41 838 1001 1384 268 1917
Fragments arranged by size:
1917 1384 1080 1001 838 612 268 41
AflII C'TTAA_G
Cuts at: 820 1017 3278 820
Size: 197 2261 4683
Fragments arranged by size:
4683 2261 197
AflIII A'CryG_T
Cuts at: 1203 3698 6040 7017 1203
Size: 2495 2342 977 1327
Fragments arranged by size:
2495 2342 1327 977
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3651 1184
Size: 2467 4674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2770 2802 2770
Size: 32 7109
AlwNI CAG_nnn'CTG
Cuts at: 3828 4884 6456 3828
Size: 1056 1572 4513
Fragments arranged by size:
4513 1572 1056
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 2207 2709 2868 2955 3081 3709 4194 4450
Size: 714 502 159 87 126 628 485 256
Cuts at: 4450 4676 4828 6937 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 714 628 502 485 256 226
159 152 126 87
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 4466 1870
Size: 73 2523 4545
Fragments arranged by size:
4545 2523 73
BamHI G'GATC_C
Cuts at: 4471 4838 4471
Size: 367 6774
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4027 4462 5917 618
Size: 325 217 627 2240 435 1455 1842
Fragments arranged by size:
2240 1842 1455 627 435 325 217
BanII G_rGCy'C
Cuts at: 1391 2239 4177 4389 4460 1391
Size: 848 1938 212 71 4072
Fragments arranged by size:
4072 1938 848 212 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 928
Size: 971 779 5391
Fragments arranged by size:
5391 971 779
BbvCI CC'TCA_GC
Cuts at: 2655 2655
Size: 7141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5332 5366 1903
Size: 34 3395 34 3678
Fragments arranged by size:
3678 3395 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7141
BfrBI ATG'CAT
Cuts at: 3437 3437
Size: 7141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2734 3470 5752
Size: 107 122 71 631 605 1062 736 2282
Cuts at: 5752 136
Size: 1525
Fragments arranged by size:
2282 1525 1062 736 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 4558 4558
Size: 7141
Bme1580I G_kGCm'C
Cuts at: 1790 5202 6358 1790
Size: 3412 1156 2573
Fragments arranged by size:
3412 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4872 5830 442
Size: 416 53 83 399 24 3455 958 1753
Fragments arranged by size:
3455 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3219 3599 3219
Size: 380 6761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 3189 5801 1239
Size: 1151 799 2612 2579
Fragments arranged by size:
2612 2579 1151 799
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 7141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3945 5266 6131 6429 3945
Size: 1321 865 298 4657
Fragments arranged by size:
4657 1321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 4094 5804 882
Size: 617 547 2048 1710 2219
Fragments arranged by size:
2219 2048 1710 617 547
BsaAI yAC'GTr
Cuts at: 493 1204 3323 4932 493
Size: 711 2119 1609 2702
Fragments arranged by size:
2702 2119 1609 711
BsaBI GATnn'nnATC
Cuts at: 7135 7135
Size: 7141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3111 4948 5330 5965
Size: 53 83 186 936 1578 1837 382 635
Cuts at: 5965 275
Size: 1451
Fragments arranged by size:
1837 1578 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5574 5972 6246 6393 6804 5574
Size: 398 274 147 411 5911
Fragments arranged by size:
5911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3918 3948 1178
Size: 30 165 30 2515 30 4371
Fragments arranged by size:
4371 2515 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7141
BseYI C'CCAG_C
Cuts at: 6344 6840 6344
Size: 496 6645
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 2454 2597 4331 4457 1163
Size: 573 613 105 143 1734 126 3847
Fragments arranged by size:
3847 1734 613 573 143 126 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4591 5352 6380 1540
Size: 275 186 2590 761 1028 2301
Fragments arranged by size:
2590 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2239 2485 3975 4132 4389 5202 5287 6358 2239
Size: 246 1490 157 257 813 85 1071 3022
Fragments arranged by size:
3022 1490 1071 813 257 246 157 85
BsmI GAATG_Cn'
Cuts at: 2469 3437 4429 4652 4745 2469
Size: 968 992 223 93 4865
Fragments arranged by size:
4865 992 968 223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2485 3975 4132 4177 4389 4460
Size: 399 449 246 1490 157 45 212 71
Cuts at: 4460 5202 5287 6358 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1490 1071 742 449 399 246 212
157 85 71 45
BspEI T'CCGG_A
Cuts at: 6804 6804
Size: 7141
BspHI T'CATG_A
Cuts at: 5030 6760 5030
Size: 1730 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4429 4496 844
Size: 3585 67 3489
Fragments arranged by size:
3585 3489 67
BsrBI CCG'CTC
Cuts at: 1394 1823 5028 1394
Size: 429 3205 3507
Fragments arranged by size:
3507 3205 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3249 3987 5636 5810 65
Size: 1183 2001 738 1649 174 1396
Fragments arranged by size:
2001 1649 1396 1183 738 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5785 5972 1700
Size: 110 3975 187 2869
Fragments arranged by size:
3975 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3925 96
Size: 3829 3312
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 2277 5201 6213 2277
Size: 2924 1012 3205
Fragments arranged by size:
3205 2924 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7141
BstBI TT'CG_AA
Cuts at: 2290 2428 5945 2290
Size: 138 3517 3486
Fragments arranged by size:
3517 3486 138
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7141
BstYI r'GATC_y
Cuts at: 1972 4471 4838 5223 5240 6692 6778 6790 7136
Size: 2499 367 385 17 1452 86 12 346
Cuts at: 7136 1972
Size: 1977
Fragments arranged by size:
2499 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3769 513
Size: 552 939 1765 3885
Fragments arranged by size:
3885 1765 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3985 487
Size: 683 56 2759 3643
Fragments arranged by size:
3643 2759 683 56
BtsI GCAGTG_nn'
Cuts at: 4657 5451 5471 4657
Size: 794 20 6327
Fragments arranged by size:
6327 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 2034 2715 4445 2034
Size: 681 1730 4730
Fragments arranged by size:
4730 1730 681
DraIII CAC_nnn'GTG
Cuts at: 2620 2620
Size: 7141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6148 809
Size: 507 4832 1802
Fragments arranged by size:
4832 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3567 4144 4588
Size: 54 1425 50 275 18 1737 577 444
Cuts at: 4588 5477 7106 8
Size: 889 1629 43
Fragments arranged by size:
1737 1629 1425 889 577 444 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2618 3366 3980 4182 5071 1353
Size: 786 479 748 614 202 889 3423
Fragments arranged by size:
3423 889 786 748 614 479 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2486 5728 6102 6248 387
Size: 1064 138 897 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 897 374 146 138
EcoICRI GAG'CTC
Cuts at: 2237 4387 2237
Size: 2150 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2712 3189 3324 3365 4203 5204
Size: 393 758 322 477 135 41 838 1001
Cuts at: 5204 5801 6588 6856 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1001 838 787 758 597 477 393
322 268 135 41
EcoRI G'AATT_C
Cuts at: 4450 4828 4450
Size: 378 6763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 3395 2656
Size: 739 6402
FspI TGC'GCA
Cuts at: 3627 5647 3627
Size: 2020 5121
HaeII r_GCGC'y
Cuts at: 1678 6288 1678
Size: 4610 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2309 2341 2371 2403 5782
Size: 32 135 32 737 32 30 32 3379
Cuts at: 5782 5814 5856 5888 1373
Size: 32 42 32 2626
Fragments arranged by size:
3379 2626 737 135 42 32 32 32
32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 4485 4731 669
Size: 988 281 2547 246 3079
Fragments arranged by size:
3079 2547 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3306 748
Size: 2558 4583
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KpnI G_GTAC'C
Cuts at: 4466 4466
Size: 7141
MfeI C'AATT_G
Cuts at: 2271 3549 4740 2271
Size: 1278 1191 4672
Fragments arranged by size:
4672 1278 1191
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2369 3624 4096 4185 6255 6439 1305
Size: 1064 1255 472 89 2070 184 2007
Fragments arranged by size:
2070 2007 1255 1064 472 184 89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3569 10
Size: 54 1425 343 1737 3582
Fragments arranged by size:
3582 1737 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5099 5458 5617 518
Size: 648 3933 359 159 2042
Fragments arranged by size:
3933 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2354 3825 4557 5234 6382 6627 1606
Size: 748 1471 732 677 1148 245 2120
Fragments arranged by size:
2120 1471 1148 748 732 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5650
Fragments arranged by size:
5650 939 552
NdeI CA'TA_TG
Cuts at: 387 3556 387
Size: 3169 3972
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7141
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NsiI A_TGCA'T
Cuts at: 3439 3439
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 2489 3287 3532 3702 4259 4290 4499
Size: 97 1260 798 245 170 557 31 209
Cuts at: 4499 6044 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1260 798 557 245 209 170
97 31
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7141
PciI A'CATG_T
Cuts at: 3698 6040 3698
Size: 2342 4799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7141
PfoI T'CCnGG_A
Cuts at: 3101 3101
Size: 7141
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5210 5242 6723 6755 3515
Size: 32 1663 32 1481 32 3901
Fragments arranged by size:
3901 1663 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7141
PsiI TTA'TAA
Cuts at: 2819 4711 2819
Size: 1892 5249
PstI C_TGCA'G
Cuts at: 830 3861 3986 4493 830
Size: 3031 125 507 3478
Fragments arranged by size:
3478 3031 507 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2354 2354
Size: 7141
SacI G_AGCT'C
Cuts at: 2239 4389 2239
Size: 2150 4991
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SapI GCTCTTCn'nnn_
Cuts at: 2618 3980 4182 2618
Size: 1362 202 5577
Fragments arranged by size:
5577 1362 202
SbfI CC_TGCA'GG
Cuts at: 4493 4493
Size: 7141
ScaI AGT'ACT
Cuts at: 3210 5389 7101 3210
Size: 2179 1712 3250
Fragments arranged by size:
3250 2179 1712
SfcI C'TryA_G
Cuts at: 826 1045 3327 3857 3982 4489 5624 6305 6496
Size: 219 2282 530 125 507 1135 681 191
Cuts at: 6496 826
Size: 1471
Fragments arranged by size:
2282 1471 1135 681 530 507 219 191
125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 4468 1872
Size: 2596 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3278 3960 5245 6146 6408 820
Size: 197 926 1335 682 1285 901 262 1553
Fragments arranged by size:
1553 1335 1285 926 901 682 262 197
SnaBI TAC'GTA
Cuts at: 493 3323 4932 493
Size: 2830 1609 2702
Fragments arranged by size:
2830 2702 1609
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 3287 4259 4499 1132
Size: 2155 972 240 3774
Fragments arranged by size:
3774 2155 972 240
SspI AAT'ATT
Cuts at: 5 52 3060 3602 5065 5
Size: 47 3008 542 1463 2081
Fragments arranged by size:
3008 2081 1463 542 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7141
StyI C'CwwG_G
Cuts at: 513 1065 2004 4580 513
Size: 552 939 2576 3074
Fragments arranged by size:
3074 2576 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 2885 5168 5185 5338 5523 5968
Size: 1255 1101 204 2283 17 153 185 445
Cuts at: 5968 325
Size: 1498
Fragments arranged by size:
2283 1498 1255 1101 445 204 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3208 3925 5387
Size: 274 80 33 51 1443 1231 717 1462
Cuts at: 5387 7099 96
Size: 1712 138
Fragments arranged by size:
1712 1462 1443 1231 717 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7101 7133 7101
Size: 32 7109
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7141
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 4466 1870
Size: 2596 4545
XmnI GAAnn'nnTTC
Cuts at: 5270 5270
Size: 7141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4949 276
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI