Restriction map for
pFN21AE6751
(Circular) (Six-base) MAPSORT of: pFN21AE6751.seq Check: 6770 from: 1 to: 6484
pFN21AE6751 6484 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 2276 3805 2276
Size: 1529 4955
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 2717 4547 5931 6199 1632
Size: 683 402 1830 1384 268 1917
Fragments arranged by size:
1917 1830 1384 683 402 268
AflII C'TTAA_G
Cuts at: 820 1017 2465 820
Size: 197 1448 4839
Fragments arranged by size:
4839 1448 197
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2822 1184
Size: 1638 4846
AlwNI CAG_nnn'CTG
Cuts at: 2740 4227 5799 2740
Size: 1487 1572 3425
Fragments arranged by size:
3425 1572 1487
ApaI G_GGCC'C
Cuts at: 2567 2567
Size: 6484
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2945 3793 4019 4171 6280 1493
Size: 1452 848 226 152 2109 1697
Fragments arranged by size:
2109 1697 1452 848 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2594 2810 3809 1870
Size: 73 651 216 999 4545
Fragments arranged by size:
4545 999 651 216 73
AvrII C'CTAG_G
Cuts at: 3077 3077
Size: 6484
BamHI G'GATC_C
Cuts at: 3073 3814 4181 3073
Size: 741 367 5376
Fragments arranged by size:
5376 741 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2187 2276 2430 2806
Size: 325 217 627 386 14 89 154 376
Cuts at: 2806 3805 5260 618
Size: 999 1455 1842
Fragments arranged by size:
1842 1455 999 627 386 376 325 217
154 89 14
BanII G_rGCy'C
Cuts at: 1391 2102 2567 2996 3503 3657 3803 1391
Size: 711 465 429 507 154 146 4072
Fragments arranged by size:
4072 711 507 465 429 154 146
BbeI G_GCGC'C
Cuts at: 2177 2177
Size: 6484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4675 4709 1903
Size: 34 50 34 2654 34 3678
Fragments arranged by size:
3678 2654 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5095 136
Size: 107 122 71 631 605 3423 1525
Fragments arranged by size:
3423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2567 2811 4545 5701 1790
Size: 400 377 244 1734 1156 2573
Fragments arranged by size:
2573 1734 1156 400 377 244
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2647 2803 3157
Size: 416 53 83 399 24 1230 156 354
Cuts at: 3157 3497 3502 3732 4215 5173 442
Size: 340 5 230 483 958 1753
Fragments arranged by size:
1753 1230 958 483 416 399 354 340
230 156 83 53 24 5
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2654 3443 5144 1239
Size: 848 567 789 1701 2579
Fragments arranged by size:
2579 1701 848 789 567
Bpu10I CC'TnA_GC
Cuts at: 3330 3330
Size: 6484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3224 3254 3631 5147 882
Size: 617 1725 30 377 1516 2219
Fragments arranged by size:
2219 1725 1516 617 377 30
BsaAI yAC'GTr
Cuts at: 493 1204 2164 2725 4275 493
Size: 711 960 561 1550 2702
Fragments arranged by size:
2702 1550 960 711 561
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2174 2214 2493 3138
Size: 53 83 186 936 641 40 279 645
Cuts at: 3138 4291 4673 5308 275
Size: 1153 382 635 1451
Fragments arranged by size:
1451 1153 936 645 641 635 382 279
186 83 53 40
BsaWI w'CCGG_w
Cuts at: 2658 2803 3777 4917 5315 5589 5736 6147 2658
Size: 145 974 1140 398 274 147 411 2995
Fragments arranged by size:
2995 1140 974 411 398 274 147 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3416 3446 1178
Size: 30 165 30 2013 30 4216
Fragments arranged by size:
4216 2013 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2762 3655 3718 1186
Size: 1576 893 63 3952
Fragments arranged by size:
3952 1576 893 63
BseYI C'CCAG_C
Cuts at: 2246 2553 3268 3520 3619 3709 5687 6183 2246
Size: 307 715 252 99 90 1978 496 2547
Fragments arranged by size:
2547 1978 715 496 307 252 99 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2383 2465 3262 1163
Size: 573 647 82 797 4385
Fragments arranged by size:
4385 797 647 573 82
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2256 2337 2350 3934 4695 5723
Size: 275 186 255 81 13 1584 761 1028
Cuts at: 5723 1540
Size: 2301
Fragments arranged by size:
2301 1584 1028 761 275 255 186 81
13
BsiHKAI G_wGCw'C
Cuts at: 2289 3331 3657 4545 4630 5701 2289
Size: 1042 326 888 85 1071 3072
Fragments arranged by size:
3072 1071 1042 888 326 85
BsmI GAATG_Cn'
Cuts at: 2799 3995 4088 2799
Size: 1196 93 5195
Fragments arranged by size:
5195 1196 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2190 2289 2567 2811 2996 3331
Size: 399 312 88 99 278 244 185 335
Cuts at: 3331 3503 3657 3803 4545 4630 5701 1391
Size: 172 154 146 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 335 312 278 244
185 172 154 146 99 88 85
BspEI T'CCGG_A
Cuts at: 2658 6147 2658
Size: 3489 2995
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 3370 3839 844
Size: 2449 77 469 3489
Fragments arranged by size:
3489 2449 469 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2601 4371 1394
Size: 429 778 1770 3507
Fragments arranged by size:
3507 1770 778 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2387 2908 2984 5128 5315 1700
Size: 110 301 276 521 76 2144 187 2869
Fragments arranged by size:
2869 2144 521 301 276 187 110 76
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 2134 1925
Size: 209 6275
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3285 794
Size: 2491 3993
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 2548 1184
Size: 1364 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 2616 3058 2616
Size: 442 6042
BstYI r'GATC_y
Cuts at: 1972 3073 3814 4181 4566 4583 6035 6121 6133
Size: 1101 741 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1101 741 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2538 2694 2538
Size: 156 6328
BtgI C'CryG_G
Cuts at: 513 1065 2319 2418 2473 3102 3479 513
Size: 552 1254 99 55 629 377 3518
Fragments arranged by size:
3518 1254 629 552 377 99 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2130 487
Size: 683 56 904 4841
Fragments arranged by size:
4841 904 683 56
BtsI GCAGTG_nn'
Cuts at: 3234 3366 4000 4794 4814 3234
Size: 132 634 794 20 4904
Fragments arranged by size:
4904 794 634 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 2437 2437
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2906 2910
Size: 54 1425 50 275 18 423 653 4
Cuts at: 2910 3037 3363 3931 4820 6449 8
Size: 127 326 568 889 1629 43
Fragments arranged by size:
1629 1425 889 653 568 423 326 275
127 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2253 3931 1537
Size: 275 441 1678 4090
Fragments arranged by size:
4090 1678 441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4414 1353
Size: 3061 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2015 2225 5071 5445 5591 387
Size: 1064 138 426 210 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 426 374 210 146 138
EcoICRI GAG'CTC
Cuts at: 3655 3655
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2315 2654 2717 3443 4547 5144
Size: 393 455 228 339 63 726 1104 597
Cuts at: 5144 5931 6199 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1104 787 726 597 455 393 339
268 228 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 3131 3637 3696 2537
Size: 594 506 59 5325
Fragments arranged by size:
5325 594 506 59
EcoO109I rG'GnC_Cy
Cuts at: 2243 3713 2243
Size: 1470 5014
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FseI GG_CCGG'CC
Cuts at: 2912 2912
Size: 6484
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2177 5631 1678
Size: 499 3454 2531
Fragments arranged by size:
3454 2531 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2590 2622 2882
Size: 32 135 32 767 32 219 32 260
Cuts at: 2882 2914 3416 3448 5125 5157 5199 5231 1373
Size: 32 502 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 767 502 260 219 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3828 4074 669
Size: 988 281 919 971 246 3079
Fragments arranged by size:
3079 988 971 919 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 2173 2173
Size: 6484
KpnI G_GTAC'C
Cuts at: 2280 3809 2280
Size: 1529 4955
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2544 5598 5782 1305
Size: 1239 3054 184 2007
Fragments arranged by size:
3054 2007 1239 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3039 3365 10
Size: 54 1425 343 1207 326 3129
Fragments arranged by size:
3129 1425 1207 343 326 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 4442 4801 4960 518
Size: 648 1638 1638 359 159 2042
Fragments arranged by size:
2042 1638 1638 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2321 2475 2674 3438 3900 4577 5725 5970
Size: 715 154 199 764 462 677 1148 245
Cuts at: 5970 1606
Size: 2120
Fragments arranged by size:
2120 1148 764 715 677 462 245 199
154
NaeI GCC'GGC
Cuts at: 2113 2910 2986 2113
Size: 797 76 5611
Fragments arranged by size:
5611 797 76
NarI GG'CG_CC
Cuts at: 2174 2174
Size: 6484
NcoI C'CATG_G
Cuts at: 513 1065 3479 513
Size: 552 2414 3518
Fragments arranged by size:
3518 2414 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 2111 2908 2984 2111
Size: 797 76 5611
Fragments arranged by size:
5611 797 76
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3476 3842 5387 1132
Size: 97 2247 366 1545 2229
Fragments arranged by size:
2247 2229 1545 366 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2961 3759 1108
Size: 1853 798 3833
Fragments arranged by size:
3833 1853 798
PfoI T'CCnGG_A
Cuts at: 2064 2379 2615 3752 2064
Size: 315 236 1137 4796
Fragments arranged by size:
4796 1137 315 236
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 3713 2243
Size: 1470 5014
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2563 2563
Size: 6484
PstI C_TGCA'G
Cuts at: 830 3579 3836 830
Size: 2749 257 3478
Fragments arranged by size:
3478 2749 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 3438 3438
Size: 6484
RsrII CG'GwC_CG
Cuts at: 3780 3780
Size: 6484
SacI G_AGCT'C
Cuts at: 3657 3657
Size: 6484
SacII CC_GC'GG
Cuts at: 2322 2476 2322
Size: 154 6330
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2089 3575 3832 4967 5648 5839 826
Size: 219 1044 1486 257 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 1044 681 257 219 191
SfoI GGC'GCC
Cuts at: 2175 2175
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 2812 3811 1872
Size: 940 999 4545
Fragments arranged by size:
4545 999 940
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 2594 4588 5489 5751 820
Size: 197 926 522 129 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 522 262 197 129
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3476 3842 1132
Size: 2344 366 3774
Fragments arranged by size:
3774 2344 366
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2544 2622 3077 3210 3479 3543 3923
Size: 552 1479 78 455 133 269 64 380
Cuts at: 3923 513
Size: 3074
Fragments arranged by size:
3074 1479 552 455 380 269 133 78
64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 2351 2364 2567 4511 4528 4681
Size: 1255 587 184 13 203 1944 17 153
Cuts at: 4681 4866 5311 325
Size: 185 445 1498
Fragments arranged by size:
1944 1498 1255 587 445 203 185 184
153 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 4730 6442
Size: 274 80 33 51 1443 216 2537 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
2537 1712 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3019 3051 6444 6476 3019
Size: 32 3393 32 3027
Fragments arranged by size:
3393 3027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2351 1316
Size: 1035 5449
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2673 3760 2673
Size: 1087 5397
XhoI C'TCGA_G
Cuts at: 1943 2594 1943
Size: 651 5833
XmaI C'CCGG_G
Cuts at: 1870 2810 3809 1870
Size: 940 999 4545
Fragments arranged by size:
4545 999 940
XmnI GAAnn'nnTTC
Cuts at: 2715 4613 2715
Size: 1898 4586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI
PacI PmlI PspXI PsrI SanDI SapI SexAI SfiI
SrfI SwaI