Restriction map for
pFN21AE6750
(Circular) (Six-base) MAPSORT of: pFN21AE6750.seq Check: 2247 from: 1 to: 6472
pFN21AE6750 6472 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 4535 5919 6187 1632
Size: 695 2208 1384 268 1917
Fragments arranged by size:
2208 1917 1384 695 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 2899 3243 5371 6348 1203
Size: 1696 344 2128 977 1327
Fragments arranged by size:
2128 1696 1327 977 344
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2275 2856 1184
Size: 1091 581 4800
Fragments arranged by size:
4800 1091 581
AlwNI CAG_nnn'CTG
Cuts at: 2043 4215 5787 2043
Size: 2172 1572 2728
Fragments arranged by size:
2728 2172 1572
ApaLI G'TGCA_C
Cuts at: 2260 4529 5685 2260
Size: 2269 1156 3047
Fragments arranged by size:
3047 2269 1156
ApoI r'AATT_y
Cuts at: 1493 3120 3781 4007 4159 6268 1493
Size: 1627 661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1627 661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2936 3173 3191 3797 1870
Size: 73 993 237 18 606 4545
Fragments arranged by size:
4545 993 606 237 73 18
BamHI G'GATC_C
Cuts at: 3000 3162 3802 4169 3000
Size: 162 640 367 5303
Fragments arranged by size:
5303 640 367 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 3280 3473 3747 3793
Size: 325 217 627 293 1200 193 274 46
Cuts at: 3793 5248 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1200 627 325 293 274 217
193 46
BanII G_rGCy'C
Cuts at: 1391 3228 3600 3791 1391
Size: 1837 372 191 4072
Fragments arranged by size:
4072 1837 372 191
BbeI G_GCGC'C
Cuts at: 2084 2084
Size: 6472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 928
Size: 971 461 5040
Fragments arranged by size:
5040 971 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 2473 1548
Size: 925 5547
BfrBI ATG'CAT
Cuts at: 3367 3367
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2702 5083 136
Size: 107 122 71 631 605 1030 2381 1525
Fragments arranged by size:
2381 1525 1030 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 2264 3174 3285 4533 5689 1790
Size: 474 910 111 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 910 474 111
BmgBI CAC'GTC
Cuts at: 1318 3019 3244 1318
Size: 1701 225 4546
Fragments arranged by size:
4546 1701 225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3094 3659 4203
Size: 416 53 83 399 24 1677 565 544
Cuts at: 4203 5161 442
Size: 958 1753
Fragments arranged by size:
1753 1677 958 565 544 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 3721 5132 1239
Size: 1143 1339 1411 2579
Fragments arranged by size:
2579 1411 1339 1143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4597 5462 5760 3538
Size: 1059 865 298 4250
Fragments arranged by size:
4250 1059 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 3552 3563 3697 5135 882
Size: 617 957 1096 11 134 1438 2219
Fragments arranged by size:
2219 1438 1096 957 617 134 11
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 3446 4279 4661
Size: 53 83 186 936 548 1365 833 382
Cuts at: 4661 5296 275
Size: 635 1451
Fragments arranged by size:
1451 1365 936 833 635 548 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2351 4905 5303 5577 5724 6135 2351
Size: 2554 398 274 147 411 2688
Fragments arranged by size:
2688 2554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 1178
Size: 30 165 30 1425 30 4792
Fragments arranged by size:
4792 1425 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2705 2870 2990 1186
Size: 1516 3 165 120 4668
Fragments arranged by size:
4668 1516 165 120 3
BseYI C'CCAG_C
Cuts at: 2553 3688 5675 6171 2553
Size: 1135 1987 496 2854
Fragments arranged by size:
2854 1987 1135 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2000 3371 1163
Size: 573 264 1371 4264
Fragments arranged by size:
4264 1371 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2117 2706 3207 3211 3922 4683
Size: 275 186 116 589 501 4 711 761
Cuts at: 4683 5711 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 711 589 501 275 186
116 4
BsiHKAI G_wGCw'C
Cuts at: 2264 2743 3201 4533 4618 5689 2264
Size: 479 458 1332 85 1071 3047
Fragments arranged by size:
3047 1332 1071 479 458 85
BsmI GAATG_Cn'
Cuts at: 2526 3983 4076 2526
Size: 1457 93 4922
Fragments arranged by size:
4922 1457 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2674 2674
Size: 6472
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2743 3174 3201 3228 3285 3600
Size: 399 474 479 431 27 27 57 315
Cuts at: 3600 3791 4533 4618 5689 1391
Size: 191 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 479 474 431 399 315
191 85 57 27 27
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3827 844
Size: 2983 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2476 4967 5141 65
Size: 1183 1228 2491 174 1396
Fragments arranged by size:
2491 1396 1228 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2680 3206 3470 5116 5303 1700
Size: 110 870 526 264 1646 187 2869
Fragments arranged by size:
2869 1646 870 526 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 2671 3229 3353 4532 5544 2671
Size: 558 124 1179 1012 3599
Fragments arranged by size:
3599 1179 1012 558 124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 2330 1184
Size: 1146 5326
BstXI CCAn_nnnn'nTGG
Cuts at: 3107 3107
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 3000 3162 3177 3802 4169 4554 4571 6023
Size: 1028 162 15 625 367 385 17 1452
Cuts at: 6023 6109 6121 6467 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1028 625 385 367 346 162
86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 3752 3752
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2950 3255 3707 513
Size: 552 1885 305 452 3278
Fragments arranged by size:
3278 1885 552 452 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2069 487
Size: 683 56 843 4890
Fragments arranged by size:
4890 843 683 56
BtsI GCAGTG_nn'
Cuts at: 3988 4782 4802 3988
Size: 794 20 5658
Fragments arranged by size:
5658 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DraIII CAC_nnn'GTG
Cuts at: 3442 3442
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 3204 3208
Size: 54 1425 50 275 18 873 501 4
Cuts at: 3208 3304 3919 4808 6437 8
Size: 96 615 889 1629 43
Fragments arranged by size:
1629 1425 889 873 615 501 275 96
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2703 3204 3208 3919 1537
Size: 275 891 501 4 711 4090
Fragments arranged by size:
4090 891 711 501 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 2936 4402 1353
Size: 1256 327 1466 3423
Fragments arranged by size:
3423 1466 1256 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 2153 5059 5433 5579 387
Size: 1064 138 506 58 2906 374 146 1280
Fragments arranged by size:
2906 1280 1064 506 374 146 138 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2382 3721 4535 5132 5919 6187
Size: 393 695 55 1339 814 597 787 268
Cuts at: 6187 1239
Size: 1524
Fragments arranged by size:
1524 1339 814 787 695 597 393 268
55
EcoNI CCTnn'n_nnAGG
Cuts at: 2213 2213
Size: 6472
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3579 3579
Size: 6472
FseI GG_CCGG'CC
Cuts at: 3210 3210
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2084 5619 1678
Size: 406 3535 2531
Fragments arranged by size:
3535 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2828 2860 5113
Size: 32 135 32 534 32 690 32 2253
Cuts at: 5113 5145 5187 5219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2253 690 534 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 3816 4062 669
Size: 988 281 640 1238 246 3079
Fragments arranged by size:
3079 1238 988 640 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KasI G'GCGC_C
Cuts at: 2080 2080
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2396 3039 5586 5770 1305
Size: 1091 643 2547 184 2007
Fragments arranged by size:
2547 2007 1091 643 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2257 3372 4430 4789 4948 518
Size: 648 1091 1115 1058 359 159 2042
Fragments arranged by size:
2042 1115 1091 1058 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2553 3186 3652 3679 3888 4565 5713 5958
Size: 947 633 466 27 209 677 1148 245
Cuts at: 5958 1606
Size: 2120
Fragments arranged by size:
2120 1148 947 677 633 466 245 209
27
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 6472
NarI GG'CG_CC
Cuts at: 2081 2081
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5920
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NsiI A_TGCA'T
Cuts at: 3369 3369
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3830 5375 1132
Size: 97 2601 1545 2229
Fragments arranged by size:
2601 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2614 1108
Size: 1506 4966
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2577 2609 3587 3619 4541 4573 6054 6086 2577
Size: 32 978 32 922 32 1481 32 2963
Fragments arranged by size:
2963 1481 978 922 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 3126 4042 3126
Size: 916 5556
PstI C_TGCA'G
Cuts at: 830 2835 3678 3717 3824 830
Size: 2005 843 39 107 3478
Fragments arranged by size:
3478 2005 843 107 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 3679 3679
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2831 3674 3713 3820 4955 5636 5827
Size: 219 1786 843 39 107 1135 681 191
Cuts at: 5827 826
Size: 1471
Fragments arranged by size:
1786 1471 1135 843 681 219 191 107
39
SfoI GGC'GCC
Cuts at: 2082 2082
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3175 3193 3799 1872
Size: 1303 18 606 4545
Fragments arranged by size:
4545 1303 606 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4576 5477 5739 820
Size: 197 926 1610 1023 901 262 1553
Fragments arranged by size:
1610 1553 1023 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 2200 4396 5
Size: 47 2148 2196 2081
Fragments arranged by size:
2196 2148 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2146 2619 3911 513
Size: 552 1081 473 1292 3074
Fragments arranged by size:
3074 1292 1081 552 473
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 2528 2726 3113 4499 4516 4669
Size: 1255 551 397 198 387 1386 17 153
Cuts at: 4669 4854 5299 325
Size: 185 445 1498
Fragments arranged by size:
1498 1386 1255 551 445 397 387 198
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2065 4718 6430
Size: 274 80 33 51 1443 88 2653 1712
Cuts at: 6430 96
Size: 138
Fragments arranged by size:
2653 1712 1443 274 138 88 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 6432 6464 3056
Size: 32 3344 32 3064
Fragments arranged by size:
3344 3064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2957 3235 2957
Size: 278 6194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3173 3191 3797 1870
Size: 1303 18 606 4545
Fragments arranged by size:
4545 1303 606 18
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstZ17I EcoICRI EcoO109I EcoRV FspAI
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI