Restriction map for
pFN21AE6748
(Circular) (Six-base) MAPSORT of: pFN21AE6748.seq Check: 8054 from: 1 to: 6346
pFN21AE6748 6346 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2973 4156 278
Size: 53 83 186 936 1437 1183 2468
Fragments arranged by size:
2468 1437 1183 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3183 3689 1937
Size: 1246 506 4594
Fragments arranged by size:
4594 1246 506
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4409 5793 6061 1632
Size: 1550 1227 1384 268 1917
Fragments arranged by size:
1917 1550 1384 1227 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 5245 6222 1203
Size: 4042 977 1327
Fragments arranged by size:
4042 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 2663 1166
Size: 1497 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6346
AlwNI CAG_nnn'CTG
Cuts at: 2458 4089 5661 2458
Size: 1631 1572 3143
Fragments arranged by size:
3143 1631 1572
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 3124 3197 3655 3881 4033 6142 1493
Size: 1631 73 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 458 226 152 73
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 3671 1870
Size: 73 1728 4545
Fragments arranged by size:
4545 1728 73
BamHI G'GATC_C
Cuts at: 3676 4043 3676
Size: 367 5979
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3398 3667 5122 618
Size: 325 217 627 1611 269 1455 1842
Fragments arranged by size:
1842 1611 1455 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2648 3637 3665 1391
Size: 1257 989 28 4072
Fragments arranged by size:
4072 1257 989 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 2588 2852 2914 928
Size: 971 510 179 264 62 4360
Fragments arranged by size:
4360 971 510 264 179 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2147 2181 4537 4571 1903
Size: 34 210 34 2356 34 3678
Fragments arranged by size:
3678 2356 210 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 2379 1548
Size: 831 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4957 136
Size: 107 122 71 631 605 3285 1525
Fragments arranged by size:
3285 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3763 3763
Size: 6346
Bme1580I G_kGCm'C
Cuts at: 1790 2561 3090 3122 3390 4407 5563 1790
Size: 771 529 32 268 1017 1156 2573
Fragments arranged by size:
2573 1156 1017 771 529 268 32
BmgBI CAC'GTC
Cuts at: 1318 2932 1318
Size: 1614 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3580 4077 5035
Size: 416 53 83 399 24 2163 497 958
Cuts at: 5035 442
Size: 1753
Fragments arranged by size:
2163 1753 958 497 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2489 3031 5006 1239
Size: 824 426 542 1975 2579
Fragments arranged by size:
2579 1975 824 542 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2771 4471 5336 5634 2762
Size: 9 1700 865 298 3474
Fragments arranged by size:
3474 1700 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 5009 882
Size: 617 918 2592 2219
Fragments arranged by size:
2592 2219 918 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 3097 4137 493
Size: 711 1290 603 1040 2702
Fragments arranged by size:
2702 1290 1040 711 603
BsaBI GATnn'nnATC
Cuts at: 2384 6340 2384
Size: 3956 2390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2910 2970 4153 4535
Size: 53 83 186 936 1377 60 1183 382
Cuts at: 4535 5170 275
Size: 635 1451
Fragments arranged by size:
1451 1377 1183 936 635 382 186 83
60 53
BsaWI w'CCGG_w
Cuts at: 2299 2799 4779 5177 5451 5598 6009 2299
Size: 500 1980 398 274 147 411 2636
Fragments arranged by size:
2636 1980 500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 3627 1186
Size: 1669 772 3905
Fragments arranged by size:
3905 1669 772
BseYI C'CCAG_C
Cuts at: 2811 5549 6045 2811
Size: 2738 496 3112
Fragments arranged by size:
3112 2738 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 1163
Size: 573 1173 4600
Fragments arranged by size:
4600 1173 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3796 4557 5585 1540
Size: 275 186 1795 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4407 4492 5563 4407
Size: 85 1071 5190
Fragments arranged by size:
5190 1071 85
BsmI GAATG_Cn'
Cuts at: 3112 3857 3950 3112
Size: 745 93 5508
Fragments arranged by size:
5508 745 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2187 2187
Size: 6346
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2561 2648 3090 3122 3390 3637 3665
Size: 399 771 87 442 32 268 247 28
Cuts at: 3665 4407 4492 5563 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 771 742 442 399 268 247
87 85 32 28
BspEI T'CCGG_A
Cuts at: 2299 2799 6009 2299
Size: 500 3210 2636
Fragments arranged by size:
3210 2636 500
BspHI T'CATG_A
Cuts at: 2388 4235 5965 2388
Size: 1847 1730 2769
Fragments arranged by size:
2769 1847 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3701 844
Size: 2857 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2688 4233 1394
Size: 429 865 1545 3507
Fragments arranged by size:
3507 1545 865 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2973 4841 5015 65
Size: 1183 1725 1868 174 1396
Fragments arranged by size:
1868 1725 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4990 5177 1700
Size: 110 3180 187 2869
Fragments arranged by size:
3180 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 4406 5418 4406
Size: 1012 5334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2887 794
Size: 2093 4253
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6346
BstXI CCAn_nnnn'nTGG
Cuts at: 2720 2720
Size: 6346
BstYI r'GATC_y
Cuts at: 1972 2604 3538 3676 4043 4428 4445 5897 5983
Size: 632 934 138 367 385 17 1452 86
Cuts at: 5983 5995 6341 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 934 632 385 367 346 138
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3071 3071
Size: 6346
BtgI C'CryG_G
Cuts at: 513 1065 2004 2913 3153 3224 3544 3628 513
Size: 552 939 909 240 71 320 84 3231
Fragments arranged by size:
3231 939 909 552 320 240 84 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 3862 4656 4676 3862
Size: 794 20 5532
Fragments arranged by size:
5532 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2201 5353 809
Size: 507 885 3152 1802
Fragments arranged by size:
3152 1802 885 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3015 3175 3365
Size: 54 1425 50 275 18 1185 160 190
Cuts at: 3365 3793 4682 6311 8
Size: 428 889 1629 43
Fragments arranged by size:
1629 1425 1185 889 428 275 190 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2636 2940 3011 4276 1353
Size: 1283 304 71 1265 3423
Fragments arranged by size:
3423 1283 1265 304 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2489 3031 3182 4409 5006 5793
Size: 393 431 426 542 151 1227 597 787
Cuts at: 5793 6061 1239
Size: 268 1524
Fragments arranged by size:
1524 1227 787 597 542 431 426 393
268 151
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 3026 2471
Size: 555 5791
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 6346
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 5493 1678
Size: 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4987 5019 5061 5093 1373
Size: 32 135 32 3415 32 42 32 2626
Fragments arranged by size:
3415 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 3690 3936 669
Size: 988 281 997 755 246 3079
Fragments arranged by size:
3079 997 988 755 281 246
HindIII A'AGCT_T
Cuts at: 748 2328 3490 748
Size: 1580 1162 3604
Fragments arranged by size:
3604 1580 1162
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 3645 5460 5644 1305
Size: 1443 897 1815 184 2007
Fragments arranged by size:
2007 1815 1443 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 3177 3367 10
Size: 54 1425 343 1185 160 190 2989
Fragments arranged by size:
2989 1425 1185 343 190 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2529 2663 4304 4663 4822 518
Size: 648 1363 134 1641 359 159 2042
Fragments arranged by size:
2042 1641 1363 648 359 159 134
MspA1I CmG'CkG
Cuts at: 1606 2041 3371 3607 3762 4439 5587 5832 1606
Size: 435 1330 236 155 677 1148 245 2120
Fragments arranged by size:
2120 1330 1148 677 435 245 236 155
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 3544 513
Size: 552 939 909 631 3315
Fragments arranged by size:
3315 939 909 631 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 3704 5249 1132
Size: 97 2475 1545 2229
Fragments arranged by size:
2475 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PmlI CAC'GTG
Cuts at: 3097 3097
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PstI C_TGCA'G
Cuts at: 830 2091 3698 830
Size: 1261 1607 3478
Fragments arranged by size:
3478 1607 1261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 2041 2041
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SapI GCTCTTCn'nnn_
Cuts at: 2636 3011 2636
Size: 375 5971
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SfcI C'TryA_G
Cuts at: 826 1045 2087 3273 3694 4829 5510 5701 826
Size: 219 1042 1186 421 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 1042 681 421 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3673 1872
Size: 1801 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 2777 4450 5351 5613 820
Size: 197 926 807 27 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 807 262 197 27
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SspI AAT'ATT
Cuts at: 5 52 2016 2251 4270 5
Size: 47 1964 235 2019 2081
Fragments arranged by size:
2081 2019 1964 235 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2004 2913 3402 3544 3785 513
Size: 552 939 909 489 142 241 3074
Fragments arranged by size:
3074 939 909 552 489 241 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3049 4373 4390 4543 4728 5173 325
Size: 1255 1469 1324 17 153 185 445 1498
Fragments arranged by size:
1498 1469 1324 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4592 6304 96
Size: 274 80 33 51 1443 2615 1712 138
Fragments arranged by size:
2615 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6306 6338 6306
Size: 32 6314
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 3671 1870
Size: 1801 4545
XmnI GAAnn'nnTTC
Cuts at: 4475 4475
Size: 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2971 4154 276
Size: 53 83 186 936 1437 1183 2468
Fragments arranged by size:
2468 1437 1183 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI