Restriction map for
pFN21AE6747
(Circular) (Six-base) MAPSORT of: pFN21AE6747.seq Check: 3101 from: 1 to: 7273
pFN21AE6747 7273 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5083 278
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2889 4534 4616 1937
Size: 952 1645 82 4594
Fragments arranged by size:
4594 1645 952 82
Acc65I G'GTAC_C
Cuts at: 4594 4594
Size: 7273
AclI AA'CG_TT
Cuts at: 1563 2430 5400 5773 1563
Size: 867 2970 373 3063
Fragments arranged by size:
3063 2970 867 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 3718 5336 6720 6988 1632
Size: 633 1453 1618 1384 268 1917
Fragments arranged by size:
1917 1618 1453 1384 633 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7076
AflIII A'CryG_T
Cuts at: 1203 2748 2943 3456 3891 6172 7149 1203
Size: 1545 195 513 435 2281 977 1327
Fragments arranged by size:
2281 1545 1327 977 513 435 195
AgeI A'CCGG_T
Cuts at: 6104 6104
Size: 7273
AhdI GACnn_n'nnGTC
Cuts at: 6002 6002
Size: 7273
AleI CACnn'nnGTG
Cuts at: 1166 2606 1166
Size: 1440 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7273
AlwNI CAG_nnn'CTG
Cuts at: 2509 3016 5016 6588 2509
Size: 507 2000 1572 3194
Fragments arranged by size:
3194 2000 1572 507
ApaLI G'TGCA_C
Cuts at: 5330 6486 5330
Size: 1156 6117
ApoI r'AATT_y
Cuts at: 1493 2153 2456 3125 3881 4475 4487 4511 4582
Size: 660 303 669 756 594 12 24 71
Cuts at: 4582 4808 4960 7069 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 669 660 594 303 226
152 71 24 12
AseI AT'TA_AT
Cuts at: 160 1032 2220 4199 5827 160
Size: 872 1188 1979 1628 1606
Fragments arranged by size:
1979 1628 1606 1188 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
AvaI C'yCGr_G
Cuts at: 1870 1943 2315 2996 3040 4598 1870
Size: 73 372 681 44 1558 4545
Fragments arranged by size:
4545 1558 681 372 73 44
BamHI G'GATC_C
Cuts at: 3111 3556 4603 4970 3111
Size: 445 1047 367 5414
Fragments arranged by size:
5414 1047 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4505 4594 6049 618
Size: 325 217 627 2718 89 1455 1842
Fragments arranged by size:
2718 1842 1455 627 325 217 89
BanII G_rGCy'C
Cuts at: 1391 3041 4592 1391
Size: 1650 1551 4072
Fragments arranged by size:
4072 1650 1551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2646 928
Size: 971 747 5555
Fragments arranged by size:
5555 971 747
BbvCI CC'TCA_GC
Cuts at: 2081 2982 2081
Size: 901 6372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5464 5498 1903
Size: 34 3527 34 3678
Fragments arranged by size:
3678 3527 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5165 6375 3794
Size: 1371 1210 4692
Fragments arranged by size:
4692 1371 1210
BclI T'GATC_A
Cuts at: 1548 4422 1548
Size: 2874 4399
BfrBI ATG'CAT
Cuts at: 4242 4242
Size: 7273
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5884 136
Size: 107 122 71 631 605 4212 1525
Fragments arranged by size:
4212 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7268 7268
Size: 7273
BlpI GC'TnA_GC
Cuts at: 4280 4690 4280
Size: 410 6863
Bme1580I G_kGCm'C
Cuts at: 1790 2066 3028 5334 6490 1790
Size: 276 962 2306 1156 2573
Fragments arranged by size:
2573 2306 1156 962 276
BmgBI CAC'GTC
Cuts at: 1318 2959 1318
Size: 1641 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2531 4477 5004
Size: 416 53 83 399 24 1114 1946 527
Cuts at: 5004 5962 442
Size: 958 1753
Fragments arranged by size:
1946 1753 1114 958 527 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 7273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3050 3460 3591 3838 5933 1239
Size: 1811 410 131 247 2095 2579
Fragments arranged by size:
2579 2095 1811 410 247 131
Bpu10I CC'TnA_GC
Cuts at: 2081 2982 2081
Size: 901 6372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5398 6263 6561 5398
Size: 865 298 6110
Fragments arranged by size:
6110 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 2112 5936 882
Size: 617 522 91 3824 2219
Fragments arranged by size:
3824 2219 617 522 91
BsaAI yAC'GTr
Cuts at: 493 1204 2749 5064 493
Size: 711 1545 2315 2702
Fragments arranged by size:
2702 2315 1545 711
BsaBI GATnn'nnATC
Cuts at: 7267 7267
Size: 7273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4362 5080 5462 6097
Size: 53 83 186 936 2829 718 382 635
Cuts at: 6097 275
Size: 1451
Fragments arranged by size:
2829 1451 936 718 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2019 4056 5706 6104 6378 6525 6936 2019
Size: 2037 1650 398 274 147 411 2356
Fragments arranged by size:
2356 2037 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4241 4271 1178
Size: 30 165 30 2838 30 4180
Fragments arranged by size:
4180 2838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 3047 1186
Size: 1603 258 5412
Fragments arranged by size:
5412 1603 258
BseYI C'CCAG_C
Cuts at: 2509 3866 3904 4108 6476 6972 2509
Size: 1357 38 204 2368 496 2810
Fragments arranged by size:
2810 2368 1357 496 204 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 2968 4521 1163
Size: 573 1083 149 1553 3915
Fragments arranged by size:
3915 1553 1083 573 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4723 5484 6512 1540
Size: 275 186 2722 761 1028 2301
Fragments arranged by size:
2722 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5334 5419 6490 5334
Size: 85 1071 6117
Fragments arranged by size:
6117 1071 85
BsmI GAATG_Cn'
Cuts at: 3564 3870 4784 4877 3564
Size: 306 914 93 5960
Fragments arranged by size:
5960 914 306 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 3028 3041 4592 5334 5419 6490
Size: 399 276 962 13 1551 742 85 1071
Cuts at: 6490 1391
Size: 2174
Fragments arranged by size:
2174 1551 1071 962 742 399 276 85
13
BspEI T'CCGG_A
Cuts at: 2019 6936 2019
Size: 4917 2356
BspHI T'CATG_A
Cuts at: 3072 3333 5162 6892 3072
Size: 261 1829 1730 3453
Fragments arranged by size:
3453 1829 1730 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3024 4628 844
Size: 2180 1604 3489
Fragments arranged by size:
3489 2180 1604
BsrBI CCG'CTC
Cuts at: 1394 1823 2779 5160 1394
Size: 429 956 2381 3507
Fragments arranged by size:
3507 2381 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2976 4103 5768 5942 65
Size: 1183 1728 1127 1665 174 1396
Fragments arranged by size:
1728 1665 1396 1183 1127 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3354 5917 6104 1700
Size: 110 1544 2563 187 2869
Fragments arranged by size:
2869 2563 1544 187 110
BsrGI T'GTAC_A
Cuts at: 96 3390 3468 96
Size: 3294 78 3901
Fragments arranged by size:
3901 3294 78
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7273
BssSI C'ACGA_G
Cuts at: 5333 6345 5333
Size: 1012 6261
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3016 794
Size: 2222 5051
BstBI TT'CG_AA
Cuts at: 6077 6077
Size: 7273
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7273
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2038
Size: 7273
BstYI r'GATC_y
Cuts at: 1972 2787 2818 3004 3111 3556 4603 4970 5355
Size: 815 31 186 107 445 1047 367 385
Cuts at: 5355 5372 6824 6910 6922 7268 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1047 815 445 385 367 346
186 107 86 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 4265 4265
Size: 7273
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5782
Fragments arranged by size:
5782 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6534
Fragments arranged by size:
6534 683 56
BtsI GCAGTG_nn'
Cuts at: 2740 4789 5583 5603 2740
Size: 2049 794 20 4410
Fragments arranged by size:
4410 2049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3996 4031 529
Size: 35 3432 35 3771
Fragments arranged by size:
3771 3432 35 35
DraI TTT'AAA
Cuts at: 4577 4577
Size: 7273
DraIII CAC_nnn'GTG
Cuts at: 4144 4144
Size: 7273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6280 809
Size: 507 4964 1802
Fragments arranged by size:
4964 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2707 3352 3462
Size: 54 1425 50 275 18 877 645 110
Cuts at: 3462 3621 4720 5609 7238 8
Size: 159 1099 889 1629 43
Fragments arranged by size:
1629 1425 1099 889 877 645 275 159
110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4720 1537
Size: 275 2908 4090
Fragments arranged by size:
4090 2908 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2165 2175 3785 3998 4122 4170 5203
Size: 701 111 10 1610 213 124 48 1033
Cuts at: 5203 1353
Size: 3423
Fragments arranged by size:
3423 1610 1033 701 213 124 111 48
10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3017 5860 6234 6380 387
Size: 1064 138 1428 2843 374 146 1280
Fragments arranged by size:
2843 1428 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 3050 3460 3591 3718 3838 5336
Size: 393 633 785 410 131 127 120 1498
Cuts at: 5336 5933 6720 6988 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1498 787 785 633 597 410 393
268 131 127 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2649 4264 2279
Size: 370 1615 5288
Fragments arranged by size:
5288 1615 370
EcoO109I rG'GnC_Cy
Cuts at: 2010 3013 3584 2010
Size: 1003 571 5699
Fragments arranged by size:
5699 1003 571
EcoRI G'AATT_C
Cuts at: 2456 4582 4960 2456
Size: 2126 378 4769
Fragments arranged by size:
4769 2126 378
FspI TGC'GCA
Cuts at: 5779 5779
Size: 7273
HaeII r_GCGC'y
Cuts at: 1678 2088 4280 6420 1678
Size: 410 2192 2140 2531
Fragments arranged by size:
2531 2192 2140 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5914 5946 5988 6020 1373
Size: 32 135 32 4342 32 42 32 2626
Fragments arranged by size:
4342 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3433 4348 4617 4863 669
Size: 988 281 1495 915 269 246 3079
Fragments arranged by size:
3079 1495 988 915 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7273
HpaI GTT'AAC
Cuts at: 4863 4863
Size: 7273
KpnI G_GTAC'C
Cuts at: 4598 4598
Size: 7273
MfeI C'AATT_G
Cuts at: 4872 4872
Size: 7273
MluI A'CGCG_T
Cuts at: 7149 7149
Size: 7273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 3148 4272 6387 6571 1305
Size: 1161 682 1124 2115 184 2007
Fragments arranged by size:
2115 2007 1161 1124 682 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2709 3623 10
Size: 54 1425 343 877 914 3660
Fragments arranged by size:
3660 1425 914 877 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 2606 3338 3689 4325 5231 5590
Size: 648 1227 213 732 351 636 906 359
Cuts at: 5590 5749 518
Size: 159 2042
Fragments arranged by size:
2042 1227 906 732 648 636 359 351
213 159
MspA1I CmG'CkG
Cuts at: 1606 2496 3553 4689 5366 6514 6759 1606
Size: 890 1057 1136 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 1057 890 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5782
Fragments arranged by size:
5782 939 552
NdeI CA'TA_TG
Cuts at: 387 3690 387
Size: 3303 3970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7273
NotI GC'GGCC_GC
Cuts at: 4720 4720
Size: 7273
NsiI A_TGCA'T
Cuts at: 4244 4244
Size: 7273
NspI r_CATG'y
Cuts at: 1132 1229 2398 2938 2947 3895 4631 6176 1132
Size: 97 1169 540 9 948 736 1545 2229
Fragments arranged by size:
2229 1545 1169 948 736 540 97 9
PasI CC'CwG_GG
Cuts at: 1348 2089 1348
Size: 741 6532
PciI A'CATG_T
Cuts at: 2943 3891 6172 2943
Size: 948 2281 4044
Fragments arranged by size:
4044 2281 948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7273
PfoI T'CCnGG_A
Cuts at: 2790 2814 3568 3768 2790
Size: 24 754 200 6295
Fragments arranged by size:
6295 754 200 24
PmeI GTTT'AAAC
Cuts at: 4577 4577
Size: 7273
PmlI CAC'GTG
Cuts at: 2749 2749
Size: 7273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5342 5374 6855 6887 5342
Size: 32 1481 32 5728
Fragments arranged by size:
5728 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 3013 2010
Size: 1003 6270
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7273
PsiI TTA'TAA
Cuts at: 4076 4843 4076
Size: 767 6506
PspXI vC'TCGA_Gb
Cuts at: 2315 2315
Size: 7273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 7241
PstI C_TGCA'G
Cuts at: 830 2058 3012 4625 830
Size: 1228 954 1613 3478
Fragments arranged by size:
3478 1613 1228 954
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7273
PvuII CAG'CTG
Cuts at: 2496 3553 2496
Size: 1057 6216
SalI G'TCGA_C
Cuts at: 1936 4615 1936
Size: 2679 4594
SanDI GG'GwC_CC
Cuts at: 2010 2010
Size: 7273
SapI GCTCTTCn'nnn_
Cuts at: 2054 2175 2054
Size: 121 7152
SbfI CC_TGCA'GG
Cuts at: 4625 4625
Size: 7273
ScaI AGT'ACT
Cuts at: 4229 5521 7233 4229
Size: 1292 1712 4269
Fragments arranged by size:
4269 1712 1292
SexAI A'CCwGG_T
Cuts at: 3756 4140 3756
Size: 384 6889
SfcI C'TryA_G
Cuts at: 826 1045 2054 2890 3008 3175 4621 5756 6437
Size: 219 1009 836 118 167 1446 1135 681
Cuts at: 6437 6628 826
Size: 191 1471
Fragments arranged by size:
1471 1446 1135 1009 836 681 219 191
167 118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7273
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7273
SmaI CCC'GGG
Cuts at: 1872 4600 1872
Size: 2728 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3040 5377 6278 6540 820
Size: 197 926 372 725 2337 901 262 1553
Fragments arranged by size:
2337 1553 926 901 725 372 262 197
SnaBI TAC'GTA
Cuts at: 493 5064 493
Size: 4571 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7273
SphI G_CATG'C
Cuts at: 1132 4631 1132
Size: 3499 3774
SspI AAT'ATT
Cuts at: 5 52 2196 3717 4197 5197 5
Size: 47 2144 1521 480 1000 2081
Fragments arranged by size:
2144 2081 1521 1000 480 47
StuI AGG'CCT
Cuts at: 1517 2103 1517
Size: 586 6687
StyI C'CwwG_G
Cuts at: 513 1065 2004 2548 3595 4712 513
Size: 552 939 544 1047 1117 3074
Fragments arranged by size:
3074 1117 1047 939 552 544
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3438 3498 5300 5317 5470 5655 6100
Size: 1255 1858 60 1802 17 153 185 445
Cuts at: 6100 325
Size: 1498
Fragments arranged by size:
1858 1802 1498 1255 445 185 153 60
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2830 3390
Size: 274 80 33 51 1443 408 445 560
Cuts at: 3390 3468 4227 5519 7231 96
Size: 78 759 1292 1712 138
Fragments arranged by size:
1712 1443 1292 759 560 445 408 274
138 80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4072 4104 7233 7265 4072
Size: 32 3129 32 4080
Fragments arranged by size:
4080 3129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3524 1316
Size: 2208 5065
XbaI T'CTAG_A
Cuts at: 2287 4609 2287
Size: 2322 4951
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4148 4148
Size: 7273
XhoI C'TCGA_G
Cuts at: 1943 2315 3040 1943
Size: 372 725 6176
Fragments arranged by size:
6176 725 372
XmaI C'CCGG_G
Cuts at: 1870 4598 1870
Size: 2728 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 4487 5402 3743
Size: 744 915 5614
Fragments arranged by size:
5614 915 744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5081 276
Size: 53 83 186 936 3547 2468
Fragments arranged by size:
3547 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI RsrII
SacI SacII SfiI SfoI SrfI SwaI