Restriction map for
pFN21AE6744
(Circular) (Six-base) MAPSORT of: pFN21AE6744.seq Check: 476 from: 1 to: 7624
pFN21AE6744 7624 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5434 278
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 2790 4967 1937
Size: 325 528 2177 4594
Fragments arranged by size:
4594 2177 528 325
Acc65I G'GTAC_C
Cuts at: 4945 4945
Size: 7624
AclI AA'CG_TT
Cuts at: 1563 5751 6124 1563
Size: 4188 373 3063
Fragments arranged by size:
4188 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3014 3219 3319 3405 3501 3531 3970 5687
Size: 1382 205 100 86 96 30 439 1717
Cuts at: 5687 7071 7339 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1717 1384 1382 439 268 205 100
96 86 30
AflII C'TTAA_G
Cuts at: 820 1017 3002 820
Size: 197 1985 5442
Fragments arranged by size:
5442 1985 197
AflIII A'CryG_T
Cuts at: 1203 2265 6523 7500 1203
Size: 1062 4258 977 1327
Fragments arranged by size:
4258 1327 1062 977
AgeI A'CCGG_T
Cuts at: 6455 6455
Size: 7624
AhdI GACnn_n'nnGTC
Cuts at: 6353 6353
Size: 7624
AleI CACnn'nnGTG
Cuts at: 1166 2516 3200 1166
Size: 1350 684 5590
Fragments arranged by size:
5590 1350 684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7624
AlwNI CAG_nnn'CTG
Cuts at: 3259 4066 5367 6939 3259
Size: 807 1301 1572 3944
Fragments arranged by size:
3944 1572 1301 807
ApaI G_GGCC'C
Cuts at: 4824 4824
Size: 7624
ApaLI G'TGCA_C
Cuts at: 5681 6837 5681
Size: 1156 6468
ApoI r'AATT_y
Cuts at: 1493 2908 3377 3472 3791 4166 4933 5159 5311
Size: 1415 469 95 319 375 767 226 152
Cuts at: 5311 7420 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1415 767 469 375 319 226
152 95
AseI AT'TA_AT
Cuts at: 160 1032 3038 6178 160
Size: 872 2006 3140 1606
Fragments arranged by size:
3140 2006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
AvaI C'yCGr_G
Cuts at: 1870 1943 3702 4949 1870
Size: 73 1759 1247 4545
Fragments arranged by size:
4545 1759 1247 73
BamHI G'GATC_C
Cuts at: 4954 5321 4954
Size: 367 7257
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 4110 4945 6400 618
Size: 325 217 627 785 1538 835 1455 1842
Fragments arranged by size:
1842 1538 1455 835 785 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3703 3789 3891 4824 4943 1391
Size: 2312 86 102 933 119 4072
Fragments arranged by size:
4072 2312 933 119 102 86
BbeI G_GCGC'C
Cuts at: 4114 4114
Size: 7624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2605 3976 4205 4630 928
Size: 971 706 1371 229 425 3922
Fragments arranged by size:
3922 1371 971 706 425 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3725 3759 5815 5849 1903
Size: 34 1788 34 2056 34 3678
Fragments arranged by size:
3678 2056 1788 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5516 6726 5516
Size: 1210 6414
BclI T'GATC_A
Cuts at: 1548 4146 1548
Size: 2598 5026
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6235 136
Size: 107 122 71 631 605 4563 1525
Fragments arranged by size:
4563 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3228 7619 3228
Size: 4391 3233
BlpI GC'TnA_GC
Cuts at: 5041 5041
Size: 7624
Bme1580I G_kGCm'C
Cuts at: 1790 4824 5685 6841 1790
Size: 3034 861 1156 2573
Fragments arranged by size:
3034 2573 1156 861
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5355 6313 442
Size: 416 53 83 399 24 3938 958 1753
Fragments arranged by size:
3938 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2653 2954 3144 3281 3717 4255 4916 6284
Size: 1414 301 190 137 436 538 661 1368
Cuts at: 6284 1239
Size: 2579
Fragments arranged by size:
2579 1414 1368 661 538 436 301 190
137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2313 3896 5749 6614 6912 2313
Size: 1583 1853 865 298 3025
Fragments arranged by size:
3025 1853 1583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 2490 4455 6287 882
Size: 617 861 130 1965 1832 2219
Fragments arranged by size:
2219 1965 1832 861 617 130
BsaAI yAC'GTr
Cuts at: 493 1204 4333 5415 493
Size: 711 3129 1082 2702
Fragments arranged by size:
3129 2702 1082 711
BsaBI GATnn'nnATC
Cuts at: 7618 7618
Size: 7624
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4111 4596 5431 5813
Size: 53 83 186 936 2578 485 835 382
Cuts at: 5813 6448 275
Size: 635 1451
Fragments arranged by size:
2578 1451 936 835 635 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6057 6455 6729 6876 7287 6057
Size: 398 274 147 411 6394
Fragments arranged by size:
6394 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7399
Fragments arranged by size:
7399 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3476 3860 4100 4829 4879 1186
Size: 2290 384 240 729 50 3931
Fragments arranged by size:
3931 2290 729 384 240 50
BseYI C'CCAG_C
Cuts at: 3362 4271 4404 4813 6827 7323 3362
Size: 909 133 409 2014 496 3663
Fragments arranged by size:
3663 2014 909 496 409 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4468 1163
Size: 573 2732 4319
Fragments arranged by size:
4319 2732 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5074 5835 6863 1540
Size: 275 186 3073 761 1028 2301
Fragments arranged by size:
3073 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2926 3703 3789 4131 4863 5685 5770 6841 2926
Size: 777 86 342 732 822 85 1071 3709
Fragments arranged by size:
3709 1071 822 777 732 342 86 85
BsmI GAATG_Cn'
Cuts at: 2531 3099 5135 5228 2531
Size: 568 2036 93 4927
Fragments arranged by size:
4927 2036 568 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2926 3703 3789 3891 4131 4824 4863
Size: 399 1136 777 86 102 240 693 39
Cuts at: 4863 4943 5685 5770 6841 1391
Size: 80 742 85 1071 2174
Fragments arranged by size:
2174 1136 1071 777 742 693 399 240
102 86 85 80 39
BspEI T'CCGG_A
Cuts at: 7287 7287
Size: 7624
BspHI T'CATG_A
Cuts at: 3117 4431 5513 7243 3117
Size: 1314 1082 1730 3498
Fragments arranged by size:
3498 1730 1314 1082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4979 844
Size: 4135 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5511 1394
Size: 429 3688 3507
Fragments arranged by size:
3688 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3499 4035 4095 6119 6293 65
Size: 1183 2251 536 60 2024 174 1396
Fragments arranged by size:
2251 2024 1396 1183 536 174 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 6268 6455 1700
Size: 110 4458 187 2869
Fragments arranged by size:
4458 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 4875 96
Size: 1911 2868 2845
Fragments arranged by size:
2868 2845 1911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7624
BssSI C'ACGA_G
Cuts at: 3185 5684 6696 3185
Size: 2499 1012 4113
Fragments arranged by size:
4113 2499 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7624
BstBI TT'CG_AA
Cuts at: 6428 6428
Size: 7624
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7624
BstXI CCAn_nnnn'nTGG
Cuts at: 4567 4567
Size: 7624
BstYI r'GATC_y
Cuts at: 1972 2388 2432 3228 3569 4954 5321 5706 5723
Size: 416 44 796 341 1385 367 385 17
Cuts at: 5723 7175 7261 7273 7619 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1385 796 416 385 367 346
341 86 44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2256 513
Size: 552 1191 5881
Fragments arranged by size:
5881 1191 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4651 487
Size: 683 56 3425 3460
Fragments arranged by size:
3460 3425 683 56
BtsI GCAGTG_nn'
Cuts at: 2487 3579 5140 5934 5954 2487
Size: 1092 1561 794 20 4157
Fragments arranged by size:
4157 1561 1092 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7589
DraI TTT'AAA
Cuts at: 3141 4928 3141
Size: 1787 5837
DraIII CAC_nnn'GTG
Cuts at: 2561 2561
Size: 7624
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6631 809
Size: 507 5315 1802
Fragments arranged by size:
5315 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2476 3973
Size: 54 1425 50 275 18 423 223 1497
Cuts at: 3973 5071 5960 7589 8
Size: 1098 889 1629 43
Fragments arranged by size:
1629 1497 1425 1098 889 423 275 223
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5071 1537
Size: 275 3259 4090
Fragments arranged by size:
4090 3259 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4077 4299 4748 5554 1353
Size: 2724 222 449 806 3423
Fragments arranged by size:
3423 2724 806 449 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 6211 6585 6731 387
Size: 1064 138 3215 1407 374 146 1280
Fragments arranged by size:
3215 1407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3701 3787 3701
Size: 86 7538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2653 2954 3014 3144 3219 3281 3319
Size: 393 1021 301 60 130 75 62 38
Cuts at: 3319 3405 3501 3531 3717 3970 4255 4916 5687
Size: 86 96 30 186 253 285 661 771
Cuts at: 5687 6284 7071 7339 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1021 787 771 661 597 393 301
285 268 253 186 130 96 86 75
62 60 38 30
EcoO109I rG'GnC_Cy
Cuts at: 4363 4821 4363
Size: 458 7166
EcoRI G'AATT_C
Cuts at: 3791 4166 4933 5311 3791
Size: 375 767 378 6104
Fragments arranged by size:
6104 767 378 375
EcoRV GAT'ATC
Cuts at: 3454 3454
Size: 7624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2729 3250 3886 2729
Size: 521 636 6467
Fragments arranged by size:
6467 636 521
FspI TGC'GCA
Cuts at: 6130 6130
Size: 7624
HaeII r_GCGC'y
Cuts at: 1678 4114 6771 1678
Size: 2436 2657 2531
Fragments arranged by size:
2657 2531 2436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 2837 2869 3423
Size: 32 135 32 696 32 537 32 554
Cuts at: 3423 3455 6265 6297 6339 6371 1373
Size: 32 2810 32 42 32 2626
Fragments arranged by size:
2810 2626 696 554 537 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3193 3331 4968 5214 669
Size: 988 281 1255 138 1637 246 3079
Fragments arranged by size:
3079 1637 1255 988 281 246 138
HindIII A'AGCT_T
Cuts at: 748 3513 748
Size: 2765 4859
HpaI GTT'AAC
Cuts at: 5214 5214
Size: 7624
KasI G'GCGC_C
Cuts at: 4110 4110
Size: 7624
KpnI G_GTAC'C
Cuts at: 4949 4949
Size: 7624
MfeI C'AATT_G
Cuts at: 5223 5223
Size: 7624
MluI A'CGCG_T
Cuts at: 7500 7500
Size: 7624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4119 6738 6922 1305
Size: 2814 2619 184 2007
Fragments arranged by size:
2814 2619 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 2478 3975 10
Size: 54 1425 343 423 223 1497 3659
Fragments arranged by size:
3659 1497 1425 423 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 2607 3200 3253 3651 3674 4430
Size: 648 1350 91 593 53 398 23 756
Cuts at: 4430 4766 5582 5941 6100 518
Size: 336 816 359 159 2042
Fragments arranged by size:
2042 1350 816 756 648 593 398 359
336 159 91 53 23
MspA1I CmG'CkG
Cuts at: 1606 3155 4233 5040 5717 6865 7110 1606
Size: 1549 1078 807 677 1148 245 2120
Fragments arranged by size:
2120 1549 1148 1078 807 677 245
NarI GG'CG_CC
Cuts at: 4111 4111
Size: 7624
NcoI C'CATG_G
Cuts at: 513 1065 2256 513
Size: 552 1191 5881
Fragments arranged by size:
5881 1191 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7624
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7624
NotI GC'GGCC_GC
Cuts at: 5071 5071
Size: 7624
NspI r_CATG'y
Cuts at: 1132 1229 2017 2269 3152 3184 4982 6527 1132
Size: 97 788 252 883 32 1798 1545 2229
Fragments arranged by size:
2229 1798 1545 883 788 252 97 32
PasI CC'CwG_GG
Cuts at: 1348 2428 1348
Size: 1080 6544
PciI A'CATG_T
Cuts at: 2265 6523 2265
Size: 4258 3366
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7624
PmeI GTTT'AAAC
Cuts at: 4928 4928
Size: 7624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5693 5725 7206 7238 5693
Size: 32 1481 32 6079
Fragments arranged by size:
6079 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4363 4363
Size: 7624
PshAI GACnn'nnGTC
Cuts at: 1613 2502 4514 1613
Size: 889 2012 4723
Fragments arranged by size:
4723 2012 889
PsiI TTA'TAA
Cuts at: 5194 5194
Size: 7624
PspOMI G'GGCC_C
Cuts at: 4820 4820
Size: 7624
PspXI vC'TCGA_Gb
Cuts at: 3702 3702
Size: 7624
PstI C_TGCA'G
Cuts at: 830 2494 4516 4976 830
Size: 1664 2022 460 3478
Fragments arranged by size:
3478 2022 1664 460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7624
PvuII CAG'CTG
Cuts at: 3155 4233 3155
Size: 1078 6546
SacI G_AGCT'C
Cuts at: 3703 3789 3703
Size: 86 7538
SalI G'TCGA_C
Cuts at: 1936 4966 1936
Size: 3030 4594
SbfI CC_TGCA'GG
Cuts at: 4976 4976
Size: 7624
ScaI AGT'ACT
Cuts at: 4177 5872 7584 4177
Size: 1695 1712 4217
Fragments arranged by size:
4217 1712 1695
SfcI C'TryA_G
Cuts at: 826 1045 2310 2490 4512 4972 6107 6788 6979
Size: 219 1265 180 2022 460 1135 681 191
Cuts at: 6979 826
Size: 1471
Fragments arranged by size:
2022 1471 1265 1135 681 460 219 191
180
SfoI GGC'GCC
Cuts at: 4112 4112
Size: 7624
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7624
SmaI CCC'GGG
Cuts at: 1872 4951 1872
Size: 3079 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2328 3002 3702 3875 5728 6629
Size: 197 926 385 674 700 173 1853 901
Cuts at: 6629 6891 820
Size: 262 1553
Fragments arranged by size:
1853 1553 926 901 700 674 385 262
197 173
SnaBI TAC'GTA
Cuts at: 493 5415 493
Size: 4922 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7624
SphI G_CATG'C
Cuts at: 1132 3152 3184 4982 1132
Size: 2020 32 1798 3774
Fragments arranged by size:
3774 2020 1798 32
SspI AAT'ATT
Cuts at: 5 52 5548 5
Size: 47 5496 2081
Fragments arranged by size:
5496 2081 47
StuI AGG'CCT
Cuts at: 1517 4901 1517
Size: 3384 4240
StyI C'CwwG_G
Cuts at: 513 1065 2256 5063 513
Size: 552 1191 2807 3074
Fragments arranged by size:
3074 2807 1191 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2492 4592 4805 5651 5668 5821
Size: 1255 470 442 2100 213 846 17 153
Cuts at: 5821 6006 6451 325
Size: 185 445 1498
Fragments arranged by size:
2100 1498 1255 846 470 445 442 213
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2150 4175
Size: 274 80 33 51 1443 30 143 2025
Cuts at: 4175 4875 5870 7582 96
Size: 700 995 1712 138
Fragments arranged by size:
2025 1712 1443 995 700 274 143 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3381 3413 4527 4559 4638 4670 7584 7616 3381
Size: 32 1114 32 79 32 2914 32 3389
Fragments arranged by size:
3389 2914 1114 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7624
XbaI T'CTAG_A
Cuts at: 4960 4960
Size: 7624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 4688 2044
Size: 2644 4980
XhoI C'TCGA_G
Cuts at: 1943 3702 1943
Size: 1759 5865
XmaI C'CCGG_G
Cuts at: 1870 4949 1870
Size: 3079 4545
XmnI GAAnn'nnTTC
Cuts at: 4637 5753 4637
Size: 1116 6508
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5432 276
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI