Restriction map for
pFN21AE6743
(Circular) (Six-base) MAPSORT of: pFN21AE6743.seq Check: 5835 from: 1 to: 6589
pFN21AE6743 6589 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2264 3599 2141
Size: 123 1335 5131
Fragments arranged by size:
5131 1335 123
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 2945 2951 3141 3649 3799 4652 6036
Size: 1220 93 6 190 508 150 853 1384
Cuts at: 6036 6304 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1220 853 508 268 190 150
93 6
AfeI AGC'GCT
Cuts at: 3878 3878
Size: 6589
AflII C'TTAA_G
Cuts at: 820 1017 3007 820
Size: 197 1990 4402
Fragments arranged by size:
4402 1990 197
AflIII A'CryG_T
Cuts at: 1203 5488 6465 1203
Size: 4285 977 1327
Fragments arranged by size:
4285 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 5318 5318
Size: 6589
AleI CACnn'nnGTG
Cuts at: 1166 3054 3065 1166
Size: 1888 11 4690
Fragments arranged by size:
4690 1888 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6589
AlwNI CAG_nnn'CTG
Cuts at: 2903 3286 3466 4332 5904 2903
Size: 383 180 866 1572 3588
Fragments arranged by size:
3588 1572 866 383 180
ApaLI G'TGCA_C
Cuts at: 3129 4646 5802 3129
Size: 1517 1156 3916
Fragments arranged by size:
3916 1517 1156
ApoI r'AATT_y
Cuts at: 1493 2511 2912 3898 4124 4276 6385 1493
Size: 1018 401 986 226 152 2109 1697
Fragments arranged by size:
2109 1697 1018 986 401 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 2801 3914 1870
Size: 73 286 572 1113 4545
Fragments arranged by size:
4545 1113 572 286 73
BamHI G'GATC_C
Cuts at: 3919 4286 3919
Size: 367 6222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2814 3785 3910 5365 618
Size: 325 217 627 1027 971 125 1455 1842
Fragments arranged by size:
1842 1455 1027 971 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 3267 3908 1391
Size: 1876 641 4072
Fragments arranged by size:
4072 1876 641
BbeI G_GCGC'C
Cuts at: 2818 3789 2818
Size: 971 5618
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 928
Size: 971 963 4655
Fragments arranged by size:
4655 971 963
BbvCI CC'TCA_GC
Cuts at: 3171 3171
Size: 6589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4481 5691 4481
Size: 1210 5379
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6589
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2496 3705 5200
Size: 107 122 71 631 605 824 1209 1495
Cuts at: 5200 136
Size: 1525
Fragments arranged by size:
1525 1495 1209 824 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 3027 4006 3027
Size: 979 5610
Bme1580I G_kGCm'C
Cuts at: 1790 2717 3133 4650 5806 1790
Size: 927 416 1517 1156 2573
Fragments arranged by size:
2573 1517 1156 927 416
BmgBI CAC'GTC
Cuts at: 1318 3493 1318
Size: 2175 4414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3850 3859 4320
Size: 416 53 83 399 24 2433 9 461
Cuts at: 4320 5278 442
Size: 958 1753
Fragments arranged by size:
2433 1753 958 461 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 2681 2687 2702 3542 3567 3868 5249
Size: 1017 425 6 15 840 25 301 1381
Cuts at: 5249 1239
Size: 2579
Fragments arranged by size:
2579 1381 1017 840 425 301 25 15
6
Bpu10I CC'TnA_GC
Cuts at: 3171 3682 3171
Size: 511 6078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 3359 4714 5579 5877 2765
Size: 594 1355 865 298 3477
Fragments arranged by size:
3477 1355 865 594 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2077 2678 5252 882
Size: 617 578 601 2574 2219
Fragments arranged by size:
2574 2219 617 601 578
BsaAI yAC'GTr
Cuts at: 493 1204 3452 4380 493
Size: 711 2248 928 2702
Fragments arranged by size:
2702 2248 928 711
BsaBI GATnn'nnATC
Cuts at: 6583 6583
Size: 6589
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2815 3786 4396 4778
Size: 53 83 186 936 1282 971 610 382
Cuts at: 4778 5413 275
Size: 635 1451
Fragments arranged by size:
1451 1282 971 936 635 610 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2139 5022 5420 5694 5841 6252 2139
Size: 2883 398 274 147 411 2476
Fragments arranged by size:
2883 2476 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2032 2062 2248 2278 2675
Size: 30 165 30 629 30 186 30 397
Cuts at: 2675 2705 1178
Size: 30 5062
Fragments arranged by size:
5062 629 397 186 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3545 3636 1186
Size: 2359 91 4139
Fragments arranged by size:
4139 2359 91
BseYI C'CCAG_C
Cuts at: 3873 5792 6288 3873
Size: 1919 496 4174
Fragments arranged by size:
4174 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6016
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 4039 4800 5828 1540
Size: 275 186 58 1980 761 1028 2301
Fragments arranged by size:
2301 1980 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 3133 3267 4650 4735 5806 3133
Size: 134 1383 85 1071 3916
Fragments arranged by size:
3916 1383 1071 134 85
BsmI GAATG_Cn'
Cuts at: 3325 4100 4193 3325
Size: 775 93 5721
Fragments arranged by size:
5721 775 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2717 3133 3267 3908 4650 4735 5806
Size: 399 927 416 134 641 742 85 1071
Cuts at: 5806 1391
Size: 2174
Fragments arranged by size:
2174 1071 927 742 641 416 399 134
85
BspEI T'CCGG_A
Cuts at: 2139 6252 2139
Size: 4113 2476
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 2264 2706 2784 3336 3599 3944 844
Size: 1297 123 442 78 552 263 345 3489
Fragments arranged by size:
3489 1297 552 442 345 263 123 78
BsrBI CCG'CTC
Cuts at: 1394 1823 2061 2145 2301 2599 4476 1394
Size: 429 238 84 156 298 1877 3507
Fragments arranged by size:
3507 1877 429 298 238 156 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 3114 3339 5084 5258 65
Size: 1183 1866 225 1745 174 1396
Fragments arranged by size:
1866 1745 1396 1183 225 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 4649 5661 4649
Size: 1012 5577
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6589
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6589
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2212
Size: 6589
BstYI r'GATC_y
Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584
Size: 1947 367 385 17 1452 86 12 346
Cuts at: 6584 1972
Size: 1977
Fragments arranged by size:
1977 1947 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3078 3210 3576 513
Size: 552 2013 132 366 3526
Fragments arranged by size:
3526 2013 552 366 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4105 4899 4919 3694
Size: 411 794 20 5364
Fragments arranged by size:
5364 794 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6554
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 3336 3697
Size: 54 1425 50 275 18 226 1280 361
Cuts at: 3697 3822 4036 4925 6554 8
Size: 125 214 889 1629 43
Fragments arranged by size:
1629 1425 1280 889 361 275 226 214
125 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2056 4036 1537
Size: 275 244 1980 4090
Fragments arranged by size:
4090 1980 275 244
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2136 2269 3589 3648 5176 5550
Size: 1064 138 547 133 1320 59 1528 374
Cuts at: 5550 5696 387
Size: 146 1280
Fragments arranged by size:
1528 1320 1280 1064 547 374 146 138
133 59
EcoICRI GAG'CTC
Cuts at: 3265 3265
Size: 6589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2681 2687 2702 2852 2945 2951
Size: 393 624 425 6 15 150 93 6
Cuts at: 2951 3141 3542 3567 3649 3799 3868 4652 5249
Size: 190 401 25 82 150 69 784 597
Cuts at: 5249 6036 6304 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 784 624 597 425 401 393
268 190 150 150 93 82 69 25
15 6 6
EcoO109I rG'GnC_Cy
Cuts at: 2127 2845 3012 3175 2127
Size: 718 167 163 5541
Fragments arranged by size:
5541 718 167 163
EcoRI G'AATT_C
Cuts at: 2511 3898 4276 2511
Size: 1387 378 4824
Fragments arranged by size:
4824 1387 378
EcoRV GAT'ATC
Cuts at: 3145 3145
Size: 6589
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 2818 3789 3880 5736 1678
Size: 1140 971 91 1856 2531
Fragments arranged by size:
2531 1856 1140 971 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5230 5262 5304 5336 1373
Size: 32 135 32 3658 32 42 32 2626
Fragments arranged by size:
3658 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3933 4179 669
Size: 988 281 1995 246 3079
Fragments arranged by size:
3079 1995 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 2814 3785 2814
Size: 971 5618
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 4188 4188
Size: 6589
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5703 5887 1305
Size: 4398 184 2007
Fragments arranged by size:
4398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4767
Fragments arranged by size:
4767 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 3054 3065 3228 3575 4547 4906
Size: 648 1044 844 11 163 347 972 359
Cuts at: 4906 5065 518
Size: 159 2042
Fragments arranged by size:
2042 1044 972 844 648 359 347 163
159 11
MspA1I CmG'CkG
Cuts at: 1606 2219 2262 2900 3747 3756 3765 4005 4682
Size: 613 43 638 847 9 9 240 677
Cuts at: 4682 5830 6075 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 638 613 245 240
43 9 9
NarI GG'CG_CC
Cuts at: 2815 3786 2815
Size: 971 5618
NcoI C'CATG_G
Cuts at: 513 1065 3078 513
Size: 552 2013 4024
Fragments arranged by size:
4024 2013 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 3947 5492 1132
Size: 97 2718 1545 2229
Fragments arranged by size:
2718 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2492 1348
Size: 1144 5445
PciI A'CATG_T
Cuts at: 5488 5488
Size: 6589
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6589
PfoI T'CCnGG_A
Cuts at: 2658 2679 2658
Size: 21 6568
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2845 3012 2845
Size: 167 6422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PstI C_TGCA'G
Cuts at: 830 2547 3207 3333 3408 3941 830
Size: 1717 660 126 75 533 3478
Fragments arranged by size:
3478 1717 660 533 126 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2219 2262 2900 2219
Size: 43 638 5908
Fragments arranged by size:
5908 638 43
SacI G_AGCT'C
Cuts at: 3267 3267
Size: 6589
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 2847 2847
Size: 6589
SfcI C'TryA_G
Cuts at: 826 1045 2543 3203 3329 3404 3416 3937 5072
Size: 219 1498 660 126 75 12 521 1135
Cuts at: 5072 5753 5944 826
Size: 681 191 1471
Fragments arranged by size:
1498 1471 1135 681 660 521 219 191
126 75 12
SfoI GGC'GCC
Cuts at: 2816 3787 2816
Size: 971 5618
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 2231 3916 1872
Size: 359 1685 4545
Fragments arranged by size:
4545 1685 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 3007 3374 4693 5594 5856
Size: 197 926 801 263 367 1319 901 262
Cuts at: 5856 820
Size: 1553
Fragments arranged by size:
1553 1319 926 901 801 367 263 262
197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 3078 4028 513
Size: 552 2013 950 3074
Fragments arranged by size:
3074 2013 950 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4616 4633 4786 4971 5416 325
Size: 1255 3036 17 153 185 445 1498
Fragments arranged by size:
3036 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3534 4835 6547
Size: 274 80 33 51 1443 1557 1301 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
1712 1557 1443 1301 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 2248 2280 2675 2707 6549 6581 2080
Size: 32 136 32 395 32 3842 32 2088
Fragments arranged by size:
3842 2088 395 136 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 2229 3914 1870
Size: 359 1685 4545
Fragments arranged by size:
4545 1685 359
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI
XcmI