Restriction map for
pFN21AE6730
(Circular) (Six-base) MAPSORT of: pFN21AE6730.seq Check: 439 from: 1 to: 7132
pFN21AE6730 7132 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2795 2838 3007 3908 2795
Size: 43 169 901 6019
Fragments arranged by size:
6019 901 169 43
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4942 278
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4475 1937
Size: 2538 4594
Acc65I G'GTAC_C
Cuts at: 4453 4453
Size: 7132
AclI AA'CG_TT
Cuts at: 1563 5259 5632 1563
Size: 3696 373 3063
Fragments arranged by size:
3696 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 4295 4363 5195 6579 6847 1632
Size: 482 2181 68 832 1384 268 1917
Fragments arranged by size:
2181 1917 1384 832 482 268 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6935
AflIII A'CryG_T
Cuts at: 1203 3588 6031 7008 1203
Size: 2385 2443 977 1327
Fragments arranged by size:
2443 2385 1327 977
AgeI A'CCGG_T
Cuts at: 5963 5963
Size: 7132
AhdI GACnn_n'nnGTC
Cuts at: 2965 5861 2965
Size: 2896 4236
AleI CACnn'nnGTG
Cuts at: 1166 2501 3928 4100 1166
Size: 1335 1427 172 4198
Fragments arranged by size:
4198 1427 1335 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7132
AlwNI CAG_nnn'CTG
Cuts at: 2047 2086 2120 2219 2759 4012 4875 6447 2047
Size: 39 34 99 540 1253 863 1572 2732
Fragments arranged by size:
2732 1572 1253 863 540 99 39 34
ApaI G_GGCC'C
Cuts at: 2023 2179 2195 2778 3168 3492 3528 2023
Size: 156 16 583 390 324 36 5627
Fragments arranged by size:
5627 583 390 324 156 36 16
ApaLI G'TGCA_C
Cuts at: 3332 5189 6345 3332
Size: 1857 1156 4119
Fragments arranged by size:
4119 1857 1156
ApoI r'AATT_y
Cuts at: 1493 4441 4667 4819 6928 1493
Size: 2948 226 152 2109 1697
Fragments arranged by size:
2948 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5686 160
Size: 872 4654 1606
Fragments arranged by size:
4654 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 3307 3432 3521 3577 3596 3878
Size: 73 996 368 125 89 56 19 282
Cuts at: 3878 3982 3992 4336 4457 1870
Size: 104 10 344 121 4545
Fragments arranged by size:
4545 996 368 344 282 125 121 104
89 73 56 19 10
BamHI G'GATC_C
Cuts at: 2076 3190 4462 4829 2076
Size: 1114 1272 367 4379
Fragments arranged by size:
4379 1272 1114 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2660 2714 2817 3514 3804
Size: 325 217 627 873 54 103 697 290
Cuts at: 3804 4453 5908 618
Size: 649 1455 1842
Fragments arranged by size:
1842 1455 873 697 649 627 325 290
217 103 54
BanII G_rGCy'C
Cuts at: 1391 2023 2179 2195 2778 3159 3168 3492 3528
Size: 632 156 16 583 381 9 324 36
Cuts at: 3528 4167 4343 4451 1391
Size: 639 176 108 4072
Fragments arranged by size:
4072 639 632 583 381 324 176 156
108 36 16 9
BbeI G_GCGC'C
Cuts at: 2821 2821
Size: 7132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6161
BbvCI CC'TCA_GC
Cuts at: 2183 2811 3459 2183
Size: 628 648 5856
Fragments arranged by size:
5856 648 628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3111 3145 5323 5357 1903
Size: 34 1174 34 2178 34 3678
Fragments arranged by size:
3678 2178 1174 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4399 5024 6234 4399
Size: 625 1210 5297
Fragments arranged by size:
5297 1210 625
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2658 3163
Size: 107 122 71 631 605 478 508 505
Cuts at: 3163 5743 136
Size: 2580 1525
Fragments arranged by size:
2580 1525 631 605 508 505 478 122
107 71
BglII A'GATC_T
Cuts at: 7127 7127
Size: 7132
BlpI GC'TnA_GC
Cuts at: 2063 4549 2063
Size: 2486 4646
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2179 2195 2274 2594 2778 3118 3168
Size: 233 156 16 79 320 184 340 50
Cuts at: 3168 3336 3492 3528 3596 3659 3807 4107 5193
Size: 168 156 36 68 63 148 300 1086
Cuts at: 5193 6349 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1086 340 320 300 233 184
168 156 156 148 79 68 63 50
36 16
BmgBI CAC'GTC
Cuts at: 1318 2563 3337 3847 1318
Size: 1245 774 510 4603
Fragments arranged by size:
4603 1245 774 510
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2959 4114 4863
Size: 416 53 83 399 24 1542 1155 749
Cuts at: 4863 5821 442
Size: 958 1753
Fragments arranged by size:
1753 1542 1155 958 749 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2240 1056
Size: 1184 5948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3173 3643 5792 1239
Size: 1934 470 2149 2579
Fragments arranged by size:
2579 2149 1934 470
Bpu10I CC'TnA_GC
Cuts at: 2183 2525 2811 2884 3459 2183
Size: 342 286 73 575 5856
Fragments arranged by size:
5856 575 342 286 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5257 6122 6420 5257
Size: 865 298 5969
Fragments arranged by size:
5969 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 2633 2851 2954 5795 882
Size: 617 624 510 218 103 2841 2219
Fragments arranged by size:
2841 2219 624 617 510 218 103
BsaAI yAC'GTr
Cuts at: 493 1204 2977 3591 4923 493
Size: 711 1773 614 1332 2702
Fragments arranged by size:
2702 1773 1332 711 614
BsaBI GATnn'nnATC
Cuts at: 7126 7126
Size: 7132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2818 2961 3822 4939
Size: 53 83 186 936 1285 143 861 1117
Cuts at: 4939 5321 5956 275
Size: 382 635 1451
Fragments arranged by size:
1451 1285 1117 936 861 635 382 186
143 83 53
BsaWI w'CCGG_w
Cuts at: 2224 3610 5565 5963 6237 6384 6795 2224
Size: 1386 1955 398 274 147 411 2561
Fragments arranged by size:
2561 1955 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2972 3002 1178
Size: 30 165 30 1569 30 5308
Fragments arranged by size:
5308 1569 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2117 2200 2463 2468 2471 2651 3056
Size: 928 3 83 263 5 3 180 405
Cuts at: 3056 3472 4175 1186
Size: 416 703 4143
Fragments arranged by size:
4143 928 703 416 405 263 180 83
5 3 3
BseYI C'CCAG_C
Cuts at: 2022 2040 2408 2512 2690 3425 3444 4400 6335
Size: 18 368 104 178 735 19 956 1935
Cuts at: 6335 6831 2022
Size: 496 2323
Fragments arranged by size:
2323 1935 956 735 496 368 178 104
19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 2810 2816 1163
Size: 573 828 246 6 5479
Fragments arranged by size:
5479 828 573 246 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 2549 4582 5343 6371 1540
Size: 275 186 252 296 2033 761 1028 2301
Fragments arranged by size:
2301 2033 1028 761 296 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 3336 3666 4167 5193 5278 6349 3336
Size: 330 501 1026 85 1071 4119
Fragments arranged by size:
4119 1071 1026 501 330 85
BsmI GAATG_Cn'
Cuts at: 4643 4736 4643
Size: 93 7039
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 7132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2179 2195 2274 2594 2778 3118
Size: 399 233 156 16 79 320 184 340
Cuts at: 3118 3159 3168 3336 3492 3528 3596 3659 3666
Size: 41 9 168 156 36 68 63 7
Cuts at: 3666 3807 4107 4167 4343 4451 5193 5278 6349
Size: 141 300 60 176 108 742 85 1071
Cuts at: 6349 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 340 320 300 233
184 176 168 156 156 141 108 85
79 68 63 60 41 36 16 9
7
BspEI T'CCGG_A
Cuts at: 2224 3610 6795 2224
Size: 1386 3185 2561
Fragments arranged by size:
3185 2561 1386
BspHI T'CATG_A
Cuts at: 3768 5021 6751 3768
Size: 1253 1730 4149
Fragments arranged by size:
4149 1730 1253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2586 2795 2838 3007 3908 4487 844
Size: 1373 369 209 43 169 901 579 3489
Fragments arranged by size:
3489 1373 901 579 369 209 169 43
BsrBI CCG'CTC
Cuts at: 1394 1823 2944 5019 1394
Size: 429 1121 2075 3507
Fragments arranged by size:
3507 2075 1121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5627 5801 65
Size: 1183 4379 174 1396
Fragments arranged by size:
4379 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 2548 2820 2868 3223 3824 5776
Size: 110 442 296 272 48 355 601 1952
Cuts at: 5776 5963 1700
Size: 187 2869
Fragments arranged by size:
2869 1952 601 442 355 296 272 187
110 48
BsrGI T'GTAC_A
Cuts at: 96 4014 96
Size: 3918 3214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7132
BssSI C'ACGA_G
Cuts at: 3130 5192 6204 3130
Size: 2062 1012 4058
Fragments arranged by size:
4058 2062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7132
BstBI TT'CG_AA
Cuts at: 5936 5936
Size: 7132
BstEII G'GTnAC_C
Cuts at: 1184 2503 4087 1184
Size: 1319 1584 4229
Fragments arranged by size:
4229 1584 1319
BstXI CCAn_nnnn'nTGG
Cuts at: 2698 4366 2698
Size: 1668 5464
BstYI r'GATC_y
Cuts at: 1972 2076 2216 3190 4204 4462 4829 5214 5231
Size: 104 140 974 1014 258 367 385 17
Cuts at: 5231 6683 6769 6781 7127 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1014 974 385 367 346 258
140 104 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2442 2442
Size: 7132
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2282 2554 3090 3637 4365
Size: 552 939 132 146 272 536 547 728
Cuts at: 4365 513
Size: 3280
Fragments arranged by size:
3280 939 728 552 547 536 272 146
132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6393
Fragments arranged by size:
6393 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2355 2592 3500 4288 4648 5442 5462 2274
Size: 81 237 908 788 360 794 20 3944
Fragments arranged by size:
3944 908 794 788 360 237 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7097
DraI TTT'AAA
Cuts at: 4436 4436
Size: 7132
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6139 809
Size: 507 4823 1802
Fragments arranged by size:
4823 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2254 2546
Size: 54 1425 50 275 18 420 4 292
Cuts at: 2546 2666 4108 4242 4579 5468 7097 8
Size: 120 1442 134 337 889 1629 43
Fragments arranged by size:
1629 1442 1425 889 420 337 292 275
134 120 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2250 2546 4579 1537
Size: 275 438 296 2033 4090
Fragments arranged by size:
4090 2033 438 296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3339 5062 1353
Size: 1986 1723 3423
Fragments arranged by size:
3423 1986 1723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5719 6093 6239 387
Size: 1064 138 4130 374 146 1280
Fragments arranged by size:
4130 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4165 4165
Size: 7132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3173 3643 4295 4363 5195 5792
Size: 393 482 1059 470 652 68 832 597
Cuts at: 5792 6579 6847 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1059 832 787 652 597 482 470
393 268 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2445 3545 2096
Size: 349 1100 5683
Fragments arranged by size:
5683 1100 349
EcoO109I rG'GnC_Cy
Cuts at: 2019 2152 2175 2176 2191 2306 2351 2426 2774
Size: 133 23 1 15 115 45 75 348
Cuts at: 2774 3165 3455 3525 3574 3643 3727 3943 2019
Size: 391 290 70 49 69 84 216 5208
Fragments arranged by size:
5208 391 348 290 216 133 115 84
75 70 69 49 45 23 15 1
EcoRI G'AATT_C
Cuts at: 4441 4819 4441
Size: 378 6754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 7132
FseI GG_CCGG'CC
Cuts at: 2256 2552 2256
Size: 296 6836
FspI TGC'GCA
Cuts at: 5638 5638
Size: 7132
HaeII r_GCGC'y
Cuts at: 1678 2821 3432 3744 6279 1678
Size: 1143 611 312 2535 2531
Fragments arranged by size:
2535 2531 1143 611 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2293 2325 2951
Size: 32 135 32 496 32 193 32 626
Cuts at: 2951 2972 2983 3004 4189 4221 5773 5805 5847
Size: 21 11 21 1185 32 1552 32 42
Cuts at: 5847 5879 1373
Size: 32 2626
Fragments arranged by size:
2626 1552 1185 626 496 193 135 42
32 32 32 32 32 32 32 21
21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4476 4722 669
Size: 988 281 2538 246 3079
Fragments arranged by size:
3079 2538 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4307 748
Size: 3559 3573
HpaI GTT'AAC
Cuts at: 4722 4722
Size: 7132
KasI G'GCGC_C
Cuts at: 2817 2817
Size: 7132
KpnI G_GTAC'C
Cuts at: 4457 4457
Size: 7132
MfeI C'AATT_G
Cuts at: 4731 4731
Size: 7132
MluI A'CGCG_T
Cuts at: 7008 7008
Size: 7132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3237 3729 4353 6246 6430 1305
Size: 1932 492 624 1893 184 2007
Fragments arranged by size:
2007 1932 1893 624 492 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4244 10
Size: 54 1425 343 2412 2898
Fragments arranged by size:
2898 2412 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3078 3928 4100 4364 5090 5449
Size: 648 1335 577 850 172 264 726 359
Cuts at: 5449 5608 518
Size: 159 2042
Fragments arranged by size:
2042 1335 850 726 648 577 359 264
172 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2626 2694 2810 3266 3448 3858 4219
Size: 438 582 68 116 456 182 410 361
Cuts at: 4219 4390 4548 5225 6373 6618 1606
Size: 171 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 456 438 410 361
245 182 171 158 116 68
NaeI GCC'GGC
Cuts at: 2254 2550 2822 3225 3826 2254
Size: 296 272 403 601 5560
Fragments arranged by size:
5560 601 403 296 272
NarI GG'CG_CC
Cuts at: 2818 2818
Size: 7132
NcoI C'CATG_G
Cuts at: 513 1065 2004 2554 513
Size: 552 939 550 5091
Fragments arranged by size:
5091 939 552 550
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7132
NgoMIV G'CCGG_C
Cuts at: 2252 2548 2820 3223 3824 2252
Size: 296 272 403 601 5560
Fragments arranged by size:
5560 601 403 296 272
NheI G'CTAG_C
Cuts at: 1052 2236 1052
Size: 1184 5948
NotI GC'GGCC_GC
Cuts at: 4579 4579
Size: 7132
NspI r_CATG'y
Cuts at: 1132 1229 3400 4490 6035 1132
Size: 97 2171 1090 1545 2229
Fragments arranged by size:
2229 2171 1545 1090 97
PasI CC'CwG_GG
Cuts at: 1348 2016 2423 2585 2734 3110 3161 1348
Size: 668 407 162 149 376 51 5319
Fragments arranged by size:
5319 668 407 376 162 149 51
PciI A'CATG_T
Cuts at: 6031 6031
Size: 7132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2120 2759 1108
Size: 1012 639 5481
Fragments arranged by size:
5481 1012 639
PfoI T'CCnGG_A
Cuts at: 2672 3339 4167 2672
Size: 667 828 5637
Fragments arranged by size:
5637 828 667
PmeI GTTT'AAAC
Cuts at: 4436 4436
Size: 7132
PmlI CAC'GTG
Cuts at: 3591 3591
Size: 7132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 6714 6746 5201
Size: 32 1481 32 5587
Fragments arranged by size:
5587 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3455 3574 3643 3727 3943 3455
Size: 119 69 84 216 6644
Fragments arranged by size:
6644 216 119 84 69
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7132
PsiI TTA'TAA
Cuts at: 4702 4702
Size: 7132
PspOMI G'GGCC_C
Cuts at: 2019 2175 2191 2774 3164 3488 3524 2019
Size: 156 16 583 390 324 36 5627
Fragments arranged by size:
5627 583 390 324 156 36 16
PspXI vC'TCGA_Gb
Cuts at: 3878 3878
Size: 7132
PstI C_TGCA'G
Cuts at: 830 2631 3507 4484 830
Size: 1801 876 977 3478
Fragments arranged by size:
3478 1801 977 876
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7132
PvuII CAG'CTG
Cuts at: 2044 2626 2694 2810 3266 3448 4219 2044
Size: 582 68 116 456 182 771 4957
Fragments arranged by size:
4957 771 582 456 182 116 68
SacI G_AGCT'C
Cuts at: 4167 4167
Size: 7132
SalI G'TCGA_C
Cuts at: 1936 4474 1936
Size: 2538 4594
SanDI GG'GwC_CC
Cuts at: 3943 3943
Size: 7132
SbfI CC_TGCA'GG
Cuts at: 4484 4484
Size: 7132
ScaI AGT'ACT
Cuts at: 5380 7092 5380
Size: 1712 5420
SfcI C'TryA_G
Cuts at: 826 1045 2627 3301 3503 3904 4351 4480 5615
Size: 219 1582 674 202 401 447 129 1135
Cuts at: 5615 6296 6487 826
Size: 681 191 1471
Fragments arranged by size:
1582 1471 1135 681 674 447 401 219
202 191 129
SfiI GGCCn_nnn'nGGCC
Cuts at: 2150 2150
Size: 7132
SfoI GGC'GCC
Cuts at: 2819 2819
Size: 7132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7132
SmaI CCC'GGG
Cuts at: 1872 3309 3523 3984 4459 1872
Size: 1437 214 461 475 4545
Fragments arranged by size:
4545 1437 475 461 214
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3878 5236 6137 6399 820
Size: 197 926 1935 1358 901 262 1553
Fragments arranged by size:
1935 1553 1358 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4923 493
Size: 4430 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7132
SphI G_CATG'C
Cuts at: 1132 4490 1132
Size: 3358 3774
SrfI GCCC'GGGC
Cuts at: 3309 3523 3309
Size: 214 6918
SspI AAT'ATT
Cuts at: 5 52 5056 5
Size: 47 5004 2081
Fragments arranged by size:
5004 2081 47
StuI AGG'CCT
Cuts at: 1517 3540 1517
Size: 2023 5109
StyI C'CwwG_G
Cuts at: 513 1065 2004 2434 2554 4571 513
Size: 552 939 430 120 2017 3074
Fragments arranged by size:
3074 2017 939 552 430 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 3940 5159 5176 5329 5514 5959
Size: 1255 842 1518 1219 17 153 185 445
Cuts at: 5959 325
Size: 1498
Fragments arranged by size:
1518 1498 1255 1219 842 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4014 5378 7090
Size: 274 80 33 51 1443 2037 1364 1712
Cuts at: 7090 96
Size: 138
Fragments arranged by size:
2037 1712 1443 1364 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7092 7124 7092
Size: 32 7100
Tth111I GACn'n_nGTC
Cuts at: 1316 2933 1316
Size: 1617 5515
XbaI T'CTAG_A
Cuts at: 4468 4468
Size: 7132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3184 3612 3680 3184
Size: 428 68 6636
Fragments arranged by size:
6636 428 68
XhoI C'TCGA_G
Cuts at: 1943 3878 1943
Size: 1935 5197
XmaI C'CCGG_G
Cuts at: 1870 3307 3521 3982 4457 1870
Size: 1437 214 461 475 4545
Fragments arranged by size:
4545 1437 475 461 214
XmnI GAAnn'nnTTC
Cuts at: 2166 5261 2166
Size: 3095 4037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4940 276
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PsrI
RsrII SacII SapI SexAI SwaI