Restriction map for
pFN21AE6729
(Circular) (Six-base) MAPSORT of: pFN21AE6729.seq Check: 2362 from: 1 to: 7960
pFN21AE6729 7960 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2438 2438
Size: 7960
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5770 278
Size: 53 83 186 936 4234 2468
Fragments arranged by size:
4234 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5303 1937
Size: 3366 4594
Acc65I G'GTAC_C
Cuts at: 5281 5281
Size: 7960
AclI AA'CG_TT
Cuts at: 1563 6087 6460 1563
Size: 4524 373 3063
Fragments arranged by size:
4524 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3236 3388 4118 6023 7407 7675 1632
Size: 1604 152 730 1905 1384 268 1917
Fragments arranged by size:
1917 1905 1604 1384 730 268 152
AfeI AGC'GCT
Cuts at: 4018 4523 4018
Size: 505 7455
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7763
AflIII A'CryG_T
Cuts at: 1203 2730 3548 4545 6859 7836 1203
Size: 1527 818 997 2314 977 1327
Fragments arranged by size:
2314 1527 1327 997 977 818
AgeI A'CCGG_T
Cuts at: 6791 6791
Size: 7960
AhdI GACnn_n'nnGTC
Cuts at: 6689 6689
Size: 7960
AleI CACnn'nnGTG
Cuts at: 1166 2021 4566 5069 1166
Size: 855 2545 503 4057
Fragments arranged by size:
4057 2545 855 503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3618 4595 1184
Size: 2434 977 4549
Fragments arranged by size:
4549 2434 977
AlwNI CAG_nnn'CTG
Cuts at: 3122 4087 4810 5703 7275 3122
Size: 965 723 893 1572 3807
Fragments arranged by size:
3807 1572 965 893 723
ApaLI G'TGCA_C
Cuts at: 4559 5024 6017 7173 4559
Size: 465 993 1156 5346
Fragments arranged by size:
5346 1156 993 465
ApoI r'AATT_y
Cuts at: 1493 2591 2828 5269 5495 5647 7756 1493
Size: 1098 237 2441 226 152 2109 1697
Fragments arranged by size:
2441 2109 1697 1098 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6514 160
Size: 872 5482 1606
Fragments arranged by size:
5482 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7960
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2952 3110 3208 5183 5285 1870
Size: 73 120 889 158 98 1975 102 4545
Fragments arranged by size:
4545 1975 889 158 120 102 98 73
BamHI G'GATC_C
Cuts at: 3996 5290 5657 3996
Size: 1294 367 6299
Fragments arranged by size:
6299 1294 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2288 2324 2347 2360
Size: 325 217 627 236 265 36 23 13
Cuts at: 2360 3425 3580 5281 6736 618
Size: 1065 155 1701 1455 1842
Fragments arranged by size:
1842 1701 1455 1065 627 325 265 236
217 155 36 23 13
BanII G_rGCy'C
Cuts at: 1391 2073 2558 5279 1391
Size: 682 485 2721 4072
Fragments arranged by size:
4072 2721 682 485
BbeI G_GCGC'C
Cuts at: 2292 2292
Size: 7960
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4436 928
Size: 971 2537 4452
Fragments arranged by size:
4452 2537 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3697 3731 6151 6185 1903
Size: 34 50 34 1676 34 2420 34 3678
Fragments arranged by size:
3678 2420 1676 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5852 7062 5852
Size: 1210 6750
BclI T'GATC_A
Cuts at: 1548 2856 1548
Size: 1308 6652
BfrBI ATG'CAT
Cuts at: 2928 4744 2928
Size: 1816 6144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3923 4356 4472
Size: 107 122 71 631 605 2251 433 116
Cuts at: 4472 6571 136
Size: 2099 1525
Fragments arranged by size:
2251 2099 1525 631 605 433 122 116
107 71
BglII A'GATC_T
Cuts at: 2670 7955 2670
Size: 5285 2675
BlpI GC'TnA_GC
Cuts at: 5377 5377
Size: 7960
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2363 2444 3497 3583 3676 4488 4563
Size: 537 36 81 1053 86 93 812 75
Cuts at: 4563 5028 6021 7177 1790
Size: 465 993 1156 2573
Fragments arranged by size:
2573 1156 1053 993 812 537 465 93
86 81 75 36
BmgBI CAC'GTC
Cuts at: 1318 2733 4546 4953 1318
Size: 1415 1813 407 4325
Fragments arranged by size:
4325 1813 1415 407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2591 3859 5691
Size: 416 53 83 399 24 1174 1268 1832
Cuts at: 5691 6649 442
Size: 958 1753
Fragments arranged by size:
1832 1753 1268 1174 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7960
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3246 3246
Size: 7960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3116 3179 3548 4064 4550 4636 4910 6620
Size: 1877 63 369 516 486 86 274 1710
Cuts at: 6620 1239
Size: 2579
Fragments arranged by size:
2579 1877 1710 516 486 369 274 86
63
Bpu10I CC'TnA_GC
Cuts at: 4013 4230 4013
Size: 217 7743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4142 4736 5225 6085 6950 7248 4142
Size: 594 489 860 865 298 4854
Fragments arranged by size:
4854 865 860 594 489 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4973 6623 882
Size: 617 3474 1650 2219
Fragments arranged by size:
3474 2219 1650 617
BsaAI yAC'GTr
Cuts at: 493 1204 3238 4120 5751 493
Size: 711 2034 882 1631 2702
Fragments arranged by size:
2702 2034 1631 882 711
BsaBI GATnn'nnATC
Cuts at: 4634 7954 4634
Size: 3320 4640
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 3342 5109 5767
Size: 53 83 186 936 756 1053 1767 658
Cuts at: 5767 6149 6784 275
Size: 382 635 1451
Fragments arranged by size:
1767 1451 1053 936 756 658 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2920 6393 6791 7065 7212 7623 2920
Size: 3473 398 274 147 411 3257
Fragments arranged by size:
3473 3257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2448 2478 2589 2619 4784
Size: 30 165 30 1045 30 111 30 2165
Cuts at: 4784 4814 4883 4913 1178
Size: 30 69 30 4225
Fragments arranged by size:
4225 2165 1045 165 111 69 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2456 3668 3892 4047 4061 4127 1186
Size: 1132 138 1212 224 155 14 66 5019
Fragments arranged by size:
5019 1212 1132 224 155 138 66 14
BseYI C'CCAG_C
Cuts at: 2351 2549 2882 2932 3012 3703 4152 4381 4508
Size: 198 333 50 80 691 449 229 127
Cuts at: 4508 4854 4991 7163 7659 2351
Size: 346 137 2172 496 2652
Fragments arranged by size:
2652 2172 691 496 449 346 333 229
198 137 127 80 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3818 4438 1163
Size: 573 2082 620 4685
Fragments arranged by size:
4685 2082 620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3054 4079 4802 5410 6171 7199
Size: 275 186 1053 1025 723 608 761 1028
Cuts at: 7199 1540
Size: 2301
Fragments arranged by size:
2301 1053 1028 1025 761 723 608 275
186
BsiHKAI G_wGCw'C
Cuts at: 2725 3132 4563 5028 6021 6106 7177 2725
Size: 407 1431 465 993 85 1071 3508
Fragments arranged by size:
3508 1431 1071 993 465 407 85
BsmI GAATG_Cn'
Cuts at: 2743 5471 5564 2743
Size: 2728 93 5139
Fragments arranged by size:
5139 2728 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2250 2250
Size: 7960
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2327 2363 2444 2558 2725 3132
Size: 399 283 254 36 81 114 167 407
Cuts at: 3132 3497 3583 3676 4488 4563 5028 5279 6021
Size: 365 86 93 812 75 465 251 742
Cuts at: 6021 6106 7177 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 812 742 465 407 399 365
283 254 251 167 114 93 86 85
81 75 36
BspEI T'CCGG_A
Cuts at: 7623 7623
Size: 7960
BspHI T'CATG_A
Cuts at: 2565 2725 2787 5849 7579 2565
Size: 160 62 3062 1730 2946
Fragments arranged by size:
3062 2946 1730 160 62
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2438 5315 844
Size: 1544 50 2877 3489
Fragments arranged by size:
3489 2877 1544 50
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 4777 4840 5209 5847 1394
Size: 429 1501 1453 63 369 638 3507
Fragments arranged by size:
3507 1501 1453 638 429 369 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2685 3600 6455 6629 65
Size: 1183 1437 915 2855 174 1396
Fragments arranged by size:
2855 1437 1396 1183 915 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3938 6604 6791 1700
Size: 110 2128 2666 187 2869
Fragments arranged by size:
2869 2666 2128 187 110
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 5407
BssHII G'CGCG_C
Cuts at: 1925 2095 1925
Size: 170 7790
BssSI C'ACGA_G
Cuts at: 3395 4481 4517 6020 7032 3395
Size: 1086 36 1503 1012 4323
Fragments arranged by size:
4323 1503 1086 1012 36
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3190 4810 794
Size: 2396 1620 3944
Fragments arranged by size:
3944 2396 1620
BstBI TT'CG_AA
Cuts at: 6764 6764
Size: 7960
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7960
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 4346 2539
Size: 1807 6153
BstYI r'GATC_y
Cuts at: 1972 2670 3096 3996 4941 5290 5657 6042 6059
Size: 698 426 900 945 349 367 385 17
Cuts at: 6059 7511 7597 7609 7955 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 945 900 698 426 385 367
349 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3809 4883 3809
Size: 1074 6886
BtgI C'CryG_G
Cuts at: 513 1065 2019 3643 4130 4374 513
Size: 552 954 1624 487 244 4099
Fragments arranged by size:
4099 1624 954 552 487 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2509 3235 3480 3679 3700 3919
Size: 683 56 1283 726 245 199 21 219
Cuts at: 3919 4480 487
Size: 561 3967
Fragments arranged by size:
3967 1283 726 683 561 245 219 199
56 21
BtsI GCAGTG_nn'
Cuts at: 3415 4932 5064 5476 6270 6290 3415
Size: 1517 132 412 794 20 5085
Fragments arranged by size:
5085 1517 794 412 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3573 3608 529
Size: 35 3009 35 4881
Fragments arranged by size:
4881 3009 35 35
DraI TTT'AAA
Cuts at: 5264 5264
Size: 7960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4691 6967 809
Size: 507 3375 2276 1802
Fragments arranged by size:
3375 2276 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3006 3375
Size: 54 1425 50 275 18 675 501 369
Cuts at: 3375 3640 3924 4076 4302 5233 5407 6296 7925
Size: 265 284 152 226 931 174 889 1629
Cuts at: 7925 8
Size: 43
Fragments arranged by size:
1629 1425 931 889 675 501 369 284
275 265 226 174 152 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4076 5407 1537
Size: 275 2264 1331 4090
Fragments arranged by size:
4090 2264 1331 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 2253 2420 2846 5890 1353
Size: 858 42 167 426 3044 3423
Fragments arranged by size:
3423 3044 858 426 167 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 6547 6921 7067 387
Size: 1064 138 630 4328 374 146 1280
Fragments arranged by size:
4328 1280 1064 630 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3116 3179 3236 3388 3548 4064 4118
Size: 393 1484 63 57 152 160 516 54
Cuts at: 4118 4550 4636 4910 6023 6620 7407 7675 1239
Size: 432 86 274 1113 597 787 268 1524
Fragments arranged by size:
1524 1484 1113 787 597 516 432 393
274 268 160 152 86 63 57 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2282 3161 3593 2282
Size: 879 432 6649
Fragments arranged by size:
6649 879 432
EcoO109I rG'GnC_Cy
Cuts at: 2461 2943 3331 3813 4052 4111 4171 4414 4684
Size: 482 388 482 239 59 60 243 270
Cuts at: 4684 4987 5200 2461
Size: 303 213 5221
Fragments arranged by size:
5221 482 482 388 303 270 243 239
213 60 59
EcoRI G'AATT_C
Cuts at: 5269 5647 5269
Size: 378 7582
FspI TGC'GCA
Cuts at: 4634 6466 4634
Size: 1832 6128
FspAI rTGC'GCAy
Cuts at: 4634 4634
Size: 7960
HaeII r_GCGC'y
Cuts at: 1678 2292 3153 3558 3857 4020 4525 7107 1678
Size: 614 861 405 299 163 505 2582 2531
Fragments arranged by size:
2582 2531 861 614 505 405 299 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4784 4816 4859 4891 6601
Size: 32 135 32 3212 32 43 32 1710
Cuts at: 6601 6633 6675 6707 1373
Size: 32 42 32 2626
Fragments arranged by size:
3212 2626 1710 135 43 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5304 5550 669
Size: 988 281 3366 246 3079
Fragments arranged by size:
3366 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7960
HpaI GTT'AAC
Cuts at: 5550 5550
Size: 7960
KasI G'GCGC_C
Cuts at: 2288 2288
Size: 7960
KpnI G_GTAC'C
Cuts at: 5285 5285
Size: 7960
MfeI C'AATT_G
Cuts at: 5559 5559
Size: 7960
MluI A'CGCG_T
Cuts at: 7836 7836
Size: 7960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7074 7258 1305
Size: 5769 184 2007
Fragments arranged by size:
5769 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 3377 3642 3926 4304
Size: 54 1425 343 1176 369 265 284 378
Cuts at: 4304 10
Size: 3666
Fragments arranged by size:
3666 1425 1176 378 369 343 284 265
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2366 2372 2513 4544 4566 4577
Size: 648 855 345 6 141 2031 22 11
Cuts at: 4577 4673 4749 5069 5918 6277 6436 518
Size: 96 76 320 849 359 159 2042
Fragments arranged by size:
2042 2031 855 849 648 359 345 320
159 141 96 76 22 11 6
MspA1I CmG'CkG
Cuts at: 1606 2549 2699 2984 3119 4084 4132 4798 4858
Size: 943 150 285 135 965 48 666 60
Cuts at: 4858 5376 6053 7201 7446 1606
Size: 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 965 943 677 666 518 285
245 150 135 60 48
NaeI GCC'GGC
Cuts at: 3940 3940
Size: 7960
NarI GG'CG_CC
Cuts at: 2289 2289
Size: 7960
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7408
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7960
NgoMIV G'CCGG_C
Cuts at: 3938 3938
Size: 7960
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7960
NotI GC'GGCC_GC
Cuts at: 4076 5407 4076
Size: 1331 6629
NsiI A_TGCA'T
Cuts at: 2930 4746 2930
Size: 1816 6144
NspI r_CATG'y
Cuts at: 1132 1229 2932 4363 4576 5062 5134 5318 6863
Size: 97 1703 1431 213 486 72 184 1545
Cuts at: 6863 1132
Size: 2229
Fragments arranged by size:
2229 1703 1545 1431 486 213 184 97
72
PasI CC'CwG_GG
Cuts at: 1348 2284 3516 1348
Size: 936 1232 5792
Fragments arranged by size:
5792 1232 936
PciI A'CATG_T
Cuts at: 6859 6859
Size: 7960
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2594 4071 4297 1108
Size: 1486 1477 226 4771
Fragments arranged by size:
4771 1486 1477 226
PfoI T'CCnGG_A
Cuts at: 3132 3450 5013 3132
Size: 318 1563 6079
Fragments arranged by size:
6079 1563 318
PmeI GTTT'AAAC
Cuts at: 5264 5264
Size: 7960
PmlI CAC'GTG
Cuts at: 4120 4120
Size: 7960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6029 6061 7542 7574 6029
Size: 32 1481 32 6415
Fragments arranged by size:
6415 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2943 3331 3813 4111 4414 4684 4987 2943
Size: 388 482 298 303 270 303 5916
Fragments arranged by size:
5916 482 388 303 303 298 270
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7960
PsiI TTA'TAA
Cuts at: 5530 5530
Size: 7960
PspXI vC'TCGA_Gb
Cuts at: 2063 5183 2063
Size: 3120 4840
PstI C_TGCA'G
Cuts at: 830 2394 4927 5312 830
Size: 1564 2533 385 3478
Fragments arranged by size:
3478 2533 1564 385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7960
PvuII CAG'CTG
Cuts at: 2549 2699 2984 3119 4084 4858 2549
Size: 150 285 135 965 774 5651
Fragments arranged by size:
5651 965 774 285 150 135
SacII CC_GC'GG
Cuts at: 4133 4133
Size: 7960
SalI G'TCGA_C
Cuts at: 1936 5302 1936
Size: 3366 4594
SanDI GG'GwC_CC
Cuts at: 4987 4987
Size: 7960
SapI GCTCTTCn'nnn_
Cuts at: 2211 2846 2211
Size: 635 7325
SbfI CC_TGCA'GG
Cuts at: 5312 5312
Size: 7960
ScaI AGT'ACT
Cuts at: 3842 6208 7920 3842
Size: 2366 1712 3882
Fragments arranged by size:
3882 2366 1712
SfcI C'TryA_G
Cuts at: 826 1045 2390 2456 2985 4923 5308 6443 7124
Size: 219 1345 66 529 1938 385 1135 681
Cuts at: 7124 7315 826
Size: 191 1471
Fragments arranged by size:
1938 1471 1345 1135 681 529 385 219
191 66
SfoI GGC'GCC
Cuts at: 2290 2290
Size: 7960
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7960
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7960
SmaI CCC'GGG
Cuts at: 1872 2954 3112 5287 1872
Size: 1082 158 2175 4545
Fragments arranged by size:
4545 2175 1082 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 4157 4715 5183 5204 6064
Size: 197 926 120 2094 558 468 21 860
Cuts at: 6064 6965 7227 820
Size: 901 262 1553
Fragments arranged by size:
2094 1553 926 901 860 558 468 262
197 120 21
SnaBI TAC'GTA
Cuts at: 493 5751 493
Size: 5258 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7960
SphI G_CATG'C
Cuts at: 1132 2932 4363 4576 5062 5318 1132
Size: 1800 1431 213 486 256 3774
Fragments arranged by size:
3774 1800 1431 486 256 213
SspI AAT'ATT
Cuts at: 5 52 5884 5
Size: 47 5832 2081
Fragments arranged by size:
5832 2081 47
StuI AGG'CCT
Cuts at: 1517 4005 1517
Size: 2488 5472
StyI C'CwwG_G
Cuts at: 513 1065 2160 3606 3967 5399 513
Size: 552 1095 1446 361 1432 3074
Fragments arranged by size:
3074 1446 1432 1095 552 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 2297 2365 3026 3068 3107 5158
Size: 1255 488 229 68 661 42 39 2051
Cuts at: 5158 5210 5987 6004 6157 6342 6787 325
Size: 52 777 17 153 185 445 1498
Fragments arranged by size:
2051 1498 1255 777 661 488 445 229
185 153 68 52 42 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 3399 3840
Size: 274 80 33 51 1443 672 750 441
Cuts at: 3840 4899 4968 6206 7918 96
Size: 1059 69 1238 1712 138
Fragments arranged by size:
1712 1443 1238 1059 750 672 441 274
138 80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2589 2621 3128 3160 7920 7952 2589
Size: 32 507 32 4760 32 2597
Fragments arranged by size:
4760 2597 507 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2947 1316
Size: 1631 6329
XbaI T'CTAG_A
Cuts at: 5296 5296
Size: 7960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2536 2653 4160 2536
Size: 117 1507 6336
Fragments arranged by size:
6336 1507 117
XhoI C'TCGA_G
Cuts at: 1943 2063 5183 1943
Size: 120 3120 4720
Fragments arranged by size:
4720 3120 120
XmaI C'CCGG_G
Cuts at: 1870 2952 3110 5285 1870
Size: 1082 158 2175 4545
Fragments arranged by size:
4545 2175 1082 158
XmnI GAAnn'nnTTC
Cuts at: 4283 4433 6089 4283
Size: 150 1656 6154
Fragments arranged by size:
6154 1656 150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5768 276
Size: 53 83 186 936 4234 2468
Fragments arranged by size:
4234 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI NruI PacI PspOMI
PsrI RsrII SacI SexAI SfiI SrfI SwaI