Restriction map for
pFN21AE6728
(Circular) (Six-base) MAPSORT of: pFN21AE6728.seq Check: 8855 from: 1 to: 7630
pFN21AE6728 7630 bp
With 182 enzymes: SgfI *
October 20, 2017 17:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5440 278
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4973 1937
Size: 3036 4594
Acc65I G'GTAC_C
Cuts at: 4951 4951
Size: 7630
AclI AA'CG_TT
Cuts at: 1563 5757 6130 1563
Size: 4194 373 3063
Fragments arranged by size:
4194 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2021 3156 3252 4796 5693 7077 7345 1632
Size: 389 1135 96 1544 897 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1135 897 389 268 96
AflII C'TTAA_G
Cuts at: 820 1017 3538 820
Size: 197 2521 4912
Fragments arranged by size:
4912 2521 197
AflIII A'CryG_T
Cuts at: 1203 2230 3982 6529 7506 1203
Size: 1027 1752 2547 977 1327
Fragments arranged by size:
2547 1752 1327 1027 977
AgeI A'CCGG_T
Cuts at: 6461 6461
Size: 7630
AhdI GACnn_n'nnGTC
Cuts at: 6359 6359
Size: 7630
AleI CACnn'nnGTG
Cuts at: 1166 3323 1166
Size: 2157 5473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7630
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 4845 4877 3668
Size: 32 1145 32 6421
Fragments arranged by size:
6421 1145 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2752 3706 5373 6945 2752
Size: 954 1667 1572 3437
Fragments arranged by size:
3437 1667 1572 954
ApaLI G'TGCA_C
Cuts at: 5687 6843 5687
Size: 1156 6474
ApoI r'AATT_y
Cuts at: 1493 2532 2894 3016 3524 3644 4163 4451 4601
Size: 1039 362 122 508 120 519 288 150
Cuts at: 4601 4939 5165 5317 7426 1493
Size: 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1039 519 508 362 338 288
226 152 150 122 120
AseI AT'TA_AT
Cuts at: 160 1032 6184 160
Size: 872 5152 1606
Fragments arranged by size:
5152 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
AvaI C'yCGr_G
Cuts at: 1870 1943 4955 1870
Size: 73 3012 4545
Fragments arranged by size:
4545 3012 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2058 2091 2058
Size: 33 7597
BamHI G'GATC_C
Cuts at: 4960 5327 4960
Size: 367 7263
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4951 6406 618
Size: 325 217 627 3164 1455 1842
Fragments arranged by size:
3164 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2286 3396 4949 1391
Size: 895 1110 1553 4072
Fragments arranged by size:
4072 1553 1110 895
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3168 928
Size: 971 1269 5390
Fragments arranged by size:
5390 1269 971
BbvCI CC'TCA_GC
Cuts at: 2088 2088
Size: 7630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5821 5855 1903
Size: 34 3884 34 3678
Fragments arranged by size:
3884 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2059 4365 4879 5522 6732 2059
Size: 2306 514 643 1210 2957
Fragments arranged by size:
2957 2306 1210 643 514
BclI T'GATC_A
Cuts at: 1548 3415 1548
Size: 1867 5763
BfrBI ATG'CAT
Cuts at: 3739 3739
Size: 7630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6241 136
Size: 107 122 71 631 605 4569 1525
Fragments arranged by size:
4569 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7625 7625
Size: 7630
BlpI GC'TnA_GC
Cuts at: 5047 5047
Size: 7630
Bme1580I G_kGCm'C
Cuts at: 1790 5691 6847 1790
Size: 3901 1156 2573
Fragments arranged by size:
3901 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2231 1318
Size: 913 6717
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3938 5361 6319
Size: 416 53 83 399 24 2521 1423 958
Cuts at: 6319 442
Size: 1753
Fragments arranged by size:
2521 1753 1423 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2245 6290 1239
Size: 1006 4045 2579
Fragments arranged by size:
4045 2579 1006
Bpu10I CC'TnA_GC
Cuts at: 2088 2088
Size: 7630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4235 4724 4811 5755 6620 6918 4235
Size: 489 87 944 865 298 4947
Fragments arranged by size:
4947 944 865 489 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6293 882
Size: 617 4794 2219
Fragments arranged by size:
4794 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4374 5421 493
Size: 711 3170 1047 2702
Fragments arranged by size:
3170 2702 1047 711
BsaBI GATnn'nnATC
Cuts at: 2853 3414 7624 2853
Size: 561 4210 2859
Fragments arranged by size:
4210 2859 561
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5437 5819 6454 275
Size: 53 83 186 936 3904 382 635 1451
Fragments arranged by size:
3904 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6063 6461 6735 6882 7293 6063
Size: 398 274 147 411 6400
Fragments arranged by size:
6400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3262 3292 1178
Size: 30 165 30 1859 30 5516
Fragments arranged by size:
5516 1859 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2270 2273 2862 3210 1186
Size: 919 165 3 589 348 5606
Fragments arranged by size:
5606 919 589 348 165 3
BseYI C'CCAG_C
Cuts at: 4524 4902 6833 7329 4524
Size: 378 1931 496 4825
Fragments arranged by size:
4825 1931 496 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 1163
Size: 573 562 6495
Fragments arranged by size:
6495 573 562
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5080 5841 6869 1540
Size: 275 186 3079 761 1028 2301
Fragments arranged by size:
3079 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 3396 4251 5691 5776 6847 2340
Size: 1056 855 1440 85 1071 3123
Fragments arranged by size:
3123 1440 1071 1056 855 85
BsmI GAATG_Cn'
Cuts at: 2762 3068 3343 5141 5234 2762
Size: 306 275 1798 93 5158
Fragments arranged by size:
5158 1798 306 275 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2221 2221
Size: 7630
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2340 3396 4251 4949 5691 5776
Size: 399 496 54 1056 855 698 742 85
Cuts at: 5776 6847 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1056 855 742 698 496 399
85 54
BspEI T'CCGG_A
Cuts at: 7293 7293
Size: 7630
BspHI T'CATG_A
Cuts at: 4647 5519 7249 4647
Size: 872 1730 5028
Fragments arranged by size:
5028 1730 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4985 844
Size: 4141 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5517 1394
Size: 429 3694 3507
Fragments arranged by size:
3694 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2340 2608 3476 6125 6299 65
Size: 1183 1092 268 868 2649 174 1396
Fragments arranged by size:
2649 1396 1183 1092 868 268 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6274 6461 1700
Size: 110 4464 187 2869
Fragments arranged by size:
4464 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7630
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7630
BssSI C'ACGA_G
Cuts at: 5690 6702 5690
Size: 1012 6618
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 2351 3745 4843 794
Size: 1449 108 1394 1098 3581
Fragments arranged by size:
3581 1449 1394 1098 108
BstBI TT'CG_AA
Cuts at: 6434 6434
Size: 7630
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7630
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 3325 2476
Size: 849 6781
BstYI r'GATC_y
Cuts at: 1972 2008 4657 4960 5327 5712 5729 7181 7267
Size: 36 2649 303 367 385 17 1452 86
Cuts at: 7267 7279 7625 1972
Size: 12 346 1977
Fragments arranged by size:
2649 1977 1452 385 367 346 303 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6139
Fragments arranged by size:
6139 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6891
Fragments arranged by size:
6891 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 2340 2636 4799 5146 5940 5960 2111
Size: 229 296 2163 347 794 20 3781
Fragments arranged by size:
3781 2163 794 347 296 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4360 4395 529
Size: 35 3796 35 3764
Fragments arranged by size:
3796 3764 35 35
DraI TTT'AAA
Cuts at: 2203 3427 4215 4934 2203
Size: 1224 788 719 4899
Fragments arranged by size:
4899 1224 788 719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6637 809
Size: 507 5321 1802
Fragments arranged by size:
5321 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5077 5966 7595
Size: 54 1425 50 275 18 3247 889 1629
Cuts at: 7595 8
Size: 43
Fragments arranged by size:
3247 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5077 1537
Size: 275 3265 4090
Fragments arranged by size:
4090 3265 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4572 5560 1353
Size: 3219 988 3423
Fragments arranged by size:
3423 3219 988
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6217 6591 6737 387
Size: 1064 138 4628 374 146 1280
Fragments arranged by size:
4628 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3394 3394
Size: 7630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2245 3156 3252 4796 5693 6290
Size: 393 389 224 911 96 1544 897 597
Cuts at: 6290 7077 7345 1239
Size: 787 268 1524
Fragments arranged by size:
1544 1524 911 897 787 597 393 389
268 224 96
EcoNI CCTnn'n_nnAGG
Cuts at: 4665 4665
Size: 7630
EcoO109I rG'GnC_Cy
Cuts at: 3314 4780 3314
Size: 1466 6164
EcoRI G'AATT_C
Cuts at: 4939 5317 4939
Size: 378 7252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3553 3553
Size: 7630
FspI TGC'GCA
Cuts at: 6136 6136
Size: 7630
HaeII r_GCGC'y
Cuts at: 1678 6777 1678
Size: 5099 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2308 2340 2940 2972 3262
Size: 32 135 32 736 32 600 32 290
Cuts at: 3262 3294 4642 4674 4684 4716 6271 6303 6345
Size: 32 1348 32 10 32 1555 32 42
Cuts at: 6345 6377 1373
Size: 32 2626
Fragments arranged by size:
2626 1555 1348 736 600 290 135 42
32 32 32 32 32 32 32 32
32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2046 2667 3627 3778 4974 5220
Size: 988 281 108 621 960 151 1196 246
Cuts at: 5220 669
Size: 3079
Fragments arranged by size:
3079 1196 988 960 621 281 246 151
108
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7630
HpaI GTT'AAC
Cuts at: 2046 2667 3627 5220 2046
Size: 621 960 1593 4456
Fragments arranged by size:
4456 1593 960 621
KpnI G_GTAC'C
Cuts at: 4955 4955
Size: 7630
MfeI C'AATT_G
Cuts at: 5229 5229
Size: 7630
MluI A'CGCG_T
Cuts at: 7506 7506
Size: 7630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3355 6744 6928 1305
Size: 2050 3389 184 2007
Fragments arranged by size:
3389 2050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5808
Fragments arranged by size:
5808 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3734 5588 5947 6106 518
Size: 648 2157 411 1854 359 159 2042
Fragments arranged by size:
2157 2042 1854 648 411 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 5046 5723 6871 7116 1606
Size: 481 2959 677 1148 245 2120
Fragments arranged by size:
2959 2120 1148 677 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6139
Fragments arranged by size:
6139 939 552
NdeI CA'TA_TG
Cuts at: 387 3796 387
Size: 3409 4221
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7630
NotI GC'GGCC_GC
Cuts at: 5077 5077
Size: 7630
NsiI A_TGCA'T
Cuts at: 3741 3741
Size: 7630
NspI r_CATG'y
Cuts at: 1132 1229 3743 3752 3784 3986 4988 6533 1132
Size: 97 2514 9 32 202 1002 1545 2229
Fragments arranged by size:
2514 2229 1545 1002 202 97 32 9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7630
PciI A'CATG_T
Cuts at: 3982 6529 3982
Size: 2547 5083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2164 2376 4477 1108
Size: 1056 212 2101 4261
Fragments arranged by size:
4261 2101 1056 212
PfoI T'CCnGG_A
Cuts at: 2418 2418
Size: 7630
PmeI GTTT'AAAC
Cuts at: 4934 4934
Size: 7630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5699 5731 7212 7244 5699
Size: 32 1481 32 6085
Fragments arranged by size:
6085 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3314 4780 3314
Size: 1466 6164
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7630
PsiI TTA'TAA
Cuts at: 2538 3003 4616 4677 5200 2538
Size: 465 1613 61 523 4968
Fragments arranged by size:
4968 1613 523 465 61
PstI C_TGCA'G
Cuts at: 830 3711 4806 4982 830
Size: 2881 1095 176 3478
Fragments arranged by size:
3478 2881 1095 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7630
PvuII CAG'CTG
Cuts at: 2087 2087
Size: 7630
SacI G_AGCT'C
Cuts at: 3396 3396
Size: 7630
SalI G'TCGA_C
Cuts at: 1936 4972 1936
Size: 3036 4594
SbfI CC_TGCA'GG
Cuts at: 4982 4982
Size: 7630
ScaI AGT'ACT
Cuts at: 5878 7590 5878
Size: 1712 5918
SfcI C'TryA_G
Cuts at: 826 1045 3707 4227 4359 4802 4907 4978 6113
Size: 219 2662 520 132 443 105 71 1135
Cuts at: 6113 6794 6985 826
Size: 681 191 1471
Fragments arranged by size:
2662 1471 1135 681 520 443 219 191
132 105 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7630
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7630
SmaI CCC'GGG
Cuts at: 1872 4957 1872
Size: 3085 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4250 4703 4826 5734 6635
Size: 197 926 1595 712 453 123 908 901
Cuts at: 6635 6897 820
Size: 262 1553
Fragments arranged by size:
1595 1553 926 908 901 712 453 262
197 123
SnaBI TAC'GTA
Cuts at: 493 5421 493
Size: 4928 2702
SpeI A'CTAG_T
Cuts at: 152 4412 152
Size: 4260 3370
SphI G_CATG'C
Cuts at: 1132 4988 1132
Size: 3856 3774
SspI AAT'ATT
Cuts at: 5 52 2146 2730 3818 5554 5
Size: 47 2094 584 1088 1736 2081
Fragments arranged by size:
2094 2081 1736 1088 584 47
StuI AGG'CCT
Cuts at: 1517 4635 1517
Size: 3118 4512
StyI C'CwwG_G
Cuts at: 513 1065 2004 5069 513
Size: 552 939 3065 3074
Fragments arranged by size:
3074 3065 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 4753 4916 5657 5674 5827 6012
Size: 1255 1914 1259 163 741 17 153 185
Cuts at: 6012 6457 325
Size: 445 1498
Fragments arranged by size:
1914 1498 1259 1255 741 445 185 163
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2191 3283 3959
Size: 274 80 33 51 1443 214 1092 676
Cuts at: 3959 5876 7588 96
Size: 1917 1712 138
Fragments arranged by size:
1917 1712 1443 1092 676 274 214 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3826 3858 7590 7622 3826
Size: 32 3732 32 3834
Fragments arranged by size:
3834 3732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7630
XbaI T'CTAG_A
Cuts at: 3289 4966 3289
Size: 1677 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 7630
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7630
XmaI C'CCGG_G
Cuts at: 1870 4955 1870
Size: 3085 4545
XmnI GAAnn'nnTTC
Cuts at: 2147 2388 4543 5759 2147
Size: 241 2155 1216 4018
Fragments arranged by size:
4018 2155 1216 241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5438 276
Size: 53 83 186 936 3904 2468
Fragments arranged by size:
3904 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI