Restriction map for
pFN21AE6727
(Circular) (Six-base) MAPSORT of: pFN21AE6727.seq Check: 4185 from: 1 to: 6841
pFN21AE6727 6841 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 278
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3066 4058 4184 1937
Size: 1129 992 126 4594
Fragments arranged by size:
4594 1129 992 126
Acc65I G'GTAC_C
Cuts at: 4162 4162
Size: 6841
AclI AA'CG_TT
Cuts at: 1563 4968 5341 1563
Size: 3405 373 3063
Fragments arranged by size:
3405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2213 3062 3901 4904 6288 6556 1632
Size: 402 179 849 839 1003 1384 268 1917
Fragments arranged by size:
1917 1384 1003 849 839 402 268 179
AflII C'TTAA_G
Cuts at: 820 1017 3755 820
Size: 197 2738 3906
Fragments arranged by size:
3906 2738 197
AflIII A'CryG_T
Cuts at: 1203 5740 6717 1203
Size: 4537 977 1327
Fragments arranged by size:
4537 1327 977
AgeI A'CCGG_T
Cuts at: 5672 5672
Size: 6841
AhdI GACnn_n'nnGTC
Cuts at: 3371 5570 3371
Size: 2199 4642
AleI CACnn'nnGTG
Cuts at: 1166 3572 1166
Size: 2406 4435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2712 1184
Size: 1528 5313
AlwNI CAG_nnn'CTG
Cuts at: 4584 6156 4584
Size: 1572 5269
ApaLI G'TGCA_C
Cuts at: 4898 6054 4898
Size: 1156 5685
ApoI r'AATT_y
Cuts at: 1493 3251 3349 3601 4150 4376 4528 6637 1493
Size: 1758 98 252 549 226 152 2109 1697
Fragments arranged by size:
2109 1758 1697 549 252 226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 5395 160
Size: 872 4363 1606
Fragments arranged by size:
4363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 4166 1870
Size: 73 2223 4545
Fragments arranged by size:
4545 2223 73
AvrII C'CTAG_G
Cuts at: 3703 3703
Size: 6841
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3483 3516 3483
Size: 33 6808
BamHI G'GATC_C
Cuts at: 4171 4538 4171
Size: 367 6474
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4162 5617 618
Size: 325 217 627 2375 1455 1842
Fragments arranged by size:
2375 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3195 3418 4160 1391
Size: 1804 223 742 4072
Fragments arranged by size:
4072 1804 742 223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2664 2852 3907 928
Size: 971 765 188 1055 3862
Fragments arranged by size:
3862 1055 971 765 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5032 5066 1903
Size: 34 3095 34 3678
Fragments arranged by size:
3678 3095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4733 5943 3713
Size: 1020 1210 4611
Fragments arranged by size:
4611 1210 1020
BclI T'GATC_A
Cuts at: 1548 2965 4093 1548
Size: 1417 1128 4296
Fragments arranged by size:
4296 1417 1128
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5452 136
Size: 107 122 71 631 605 3780 1525
Fragments arranged by size:
3780 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6836 6836
Size: 6841
BlpI GC'TnA_GC
Cuts at: 4258 4258
Size: 6841
Bme1580I G_kGCm'C
Cuts at: 1790 3956 4902 6058 1790
Size: 2166 946 1156 2573
Fragments arranged by size:
2573 2166 1156 946
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6841
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2803 3056 3347
Size: 416 53 83 399 24 1386 253 291
Cuts at: 3347 3959 4572 5530 442
Size: 612 613 958 1753
Fragments arranged by size:
1753 1386 958 613 612 416 399 291
253 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2445 2445
Size: 6841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3154 3801 5501 1239
Size: 1915 647 1700 2579
Fragments arranged by size:
2579 1915 1700 647
Bpu10I CC'TnA_GC
Cuts at: 3971 3971
Size: 6841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3421 4966 5831 6129 3421
Size: 1545 865 298 4133
Fragments arranged by size:
4133 1545 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5504 882
Size: 617 4005 2219
Fragments arranged by size:
4005 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4632 493
Size: 711 3428 2702
Fragments arranged by size:
3428 2702 711
BsaBI GATnn'nnATC
Cuts at: 6835 6835
Size: 6841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4648 5030 5665 275
Size: 53 83 186 936 3115 382 635 1451
Fragments arranged by size:
3115 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3596 5274 5672 5946 6093 6504 3596
Size: 1678 398 274 147 411 3933
Fragments arranged by size:
3933 1678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6616
Fragments arranged by size:
6616 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6841
BseYI C'CCAG_C
Cuts at: 3811 6044 6540 3811
Size: 2233 496 4112
Fragments arranged by size:
4112 2233 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2708 3550 1163
Size: 573 972 842 4454
Fragments arranged by size:
4454 972 842 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4291 5052 6080 1540
Size: 275 186 2290 761 1028 2301
Fragments arranged by size:
2301 2290 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2201 3418 4902 4987 6058 2201
Size: 1217 1484 85 1071 2984
Fragments arranged by size:
2984 1484 1217 1071 85
BsmI GAATG_Cn'
Cuts at: 4352 4445 4352
Size: 93 6748
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 3195 3418 3956 4160 4902 4987
Size: 399 411 994 223 538 204 742 85
Cuts at: 4987 6058 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 994 742 538 411 399 223
204 85
BspEI T'CCGG_A
Cuts at: 3596 6504 3596
Size: 2908 3933
BspHI T'CATG_A
Cuts at: 3406 4730 6460 3406
Size: 1324 1730 3787
Fragments arranged by size:
3787 1730 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4196 844
Size: 3352 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 1394
Size: 429 2905 3507
Fragments arranged by size:
3507 2905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 2628 3975 5336 5510 65
Size: 1183 1143 237 1347 1361 174 1396
Fragments arranged by size:
1396 1361 1347 1183 1143 237 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5485 5672 1700
Size: 110 3675 187 2869
Fragments arranged by size:
3675 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3083 96
Size: 2987 3854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 4901 5913 4901
Size: 1012 5829
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6841
BstBI TT'CG_AA
Cuts at: 5645 5645
Size: 6841
BstEII G'GTnAC_C
Cuts at: 1184 3308 1184
Size: 2124 4717
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2176
Size: 6841
BstYI r'GATC_y
Cuts at: 1972 2803 2906 4171 4538 4923 4940 6392 6478
Size: 831 103 1265 367 385 17 1452 86
Cuts at: 6478 6490 6836 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1265 831 385 367 346 103
86 17 12
BstZ17I GTA'TAC
Cuts at: 3067 3067
Size: 6841
Bsu36I CC'TnA_GG
Cuts at: 3607 3607
Size: 6841
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5350
Fragments arranged by size:
5350 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6102
Fragments arranged by size:
6102 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 3216 4357 5151 5171 2372
Size: 844 1141 794 20 4042
Fragments arranged by size:
4042 1141 844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3520 3555 529
Size: 35 2956 35 3815
Fragments arranged by size:
3815 2956 35 35
DraI TTT'AAA
Cuts at: 2080 3868 3996 4145 2080
Size: 1788 128 149 4776
Fragments arranged by size:
4776 1788 149 128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5848 809
Size: 507 4532 1802
Fragments arranged by size:
4532 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4118 4288 5177
Size: 54 1425 50 275 18 2288 170 889
Cuts at: 5177 6806 8
Size: 1629 43
Fragments arranged by size:
2288 1629 1425 889 275 170 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4288 1537
Size: 275 2476 4090
Fragments arranged by size:
4090 2476 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2560 4771 1353
Size: 836 371 2211 3423
Fragments arranged by size:
3423 2211 836 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5428 5802 5948 387
Size: 1064 138 3839 374 146 1280
Fragments arranged by size:
3839 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3416 3416
Size: 6841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2213 3062 3154 3801 3901 4904
Size: 393 402 179 849 92 647 100 1003
Cuts at: 4904 5501 6288 6556 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1003 849 787 647 597 402 393
268 179 100 92
EcoNI CCTnn'n_nnAGG
Cuts at: 3532 3532
Size: 6841
EcoRI G'AATT_C
Cuts at: 4150 4528 4150
Size: 378 6463
EcoRV GAT'ATC
Cuts at: 2230 2230
Size: 6841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2091 2155 2091
Size: 64 6777
FspI TGC'GCA
Cuts at: 5347 5347
Size: 6841
HaeII r_GCGC'y
Cuts at: 1678 5988 1678
Size: 4310 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2537 2569 3285
Size: 32 135 32 428 32 505 32 716
Cuts at: 3285 3317 3357 3389 3419 3451 5482 5514 5556
Size: 32 40 32 30 32 2031 32 42
Cuts at: 5556 5588 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 716 505 428 135 42 40
32 32 32 32 32 32 32 32
32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 4185 4431 669
Size: 988 281 820 1427 246 3079
Fragments arranged by size:
3079 1427 988 820 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6841
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KpnI G_GTAC'C
Cuts at: 4166 4166
Size: 6841
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 2347 3555 5955 6139 1305
Size: 739 303 1208 2400 184 2007
Fragments arranged by size:
2400 2007 1208 739 303 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4120 10
Size: 54 1425 343 2288 2731
Fragments arranged by size:
2731 2288 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 4799 5158 5317 518
Size: 648 2406 1227 359 159 2042
Fragments arranged by size:
2406 2042 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2870 4257 4934 6082 6327 1606
Size: 1264 1387 677 1148 245 2120
Fragments arranged by size:
2120 1387 1264 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5350
Fragments arranged by size:
5350 939 552
NdeI CA'TA_TG
Cuts at: 387 3496 387
Size: 3109 3732
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6841
NotI GC'GGCC_GC
Cuts at: 4288 4288
Size: 6841
NspI r_CATG'y
Cuts at: 1132 1229 4199 5744 1132
Size: 97 2970 1545 2229
Fragments arranged by size:
2970 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2989 1348
Size: 1641 5200
PciI A'CATG_T
Cuts at: 5740 5740
Size: 6841
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2120 2717 4116 1108
Size: 1012 597 1399 3833
Fragments arranged by size:
3833 1399 1012 597
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3402 3434 4910 4942 6423 6455 3402
Size: 32 1476 32 1481 32 3788
Fragments arranged by size:
3788 1481 1476 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6841
PsiI TTA'TAA
Cuts at: 2291 4031 4411 2291
Size: 1740 380 4721
Fragments arranged by size:
4721 1740 380
PstI C_TGCA'G
Cuts at: 830 4193 830
Size: 3363 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
PvuII CAG'CTG
Cuts at: 2870 2870
Size: 6841
SacI G_AGCT'C
Cuts at: 3418 3418
Size: 6841
SalI G'TCGA_C
Cuts at: 1936 4183 1936
Size: 2247 4594
SapI GCTCTTCn'nnn_
Cuts at: 2189 2560 2189
Size: 371 6470
SbfI CC_TGCA'GG
Cuts at: 4193 4193
Size: 6841
ScaI AGT'ACT
Cuts at: 3984 5089 6801 3984
Size: 1105 1712 4024
Fragments arranged by size:
4024 1712 1105
SfcI C'TryA_G
Cuts at: 826 1045 4189 5324 6005 6196 826
Size: 219 3144 1135 681 191 1471
Fragments arranged by size:
3144 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 4168 1872
Size: 2296 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3436 3755 4945 5846 6108 820
Size: 197 926 1493 319 1190 901 262 1553
Fragments arranged by size:
1553 1493 1190 926 901 319 262 197
SnaBI TAC'GTA
Cuts at: 493 4632 493
Size: 4139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 4199 1132
Size: 3067 3774
SspI AAT'ATT
Cuts at: 5 52 3225 3550 4765 5
Size: 47 3173 325 1215 2081
Fragments arranged by size:
3173 2081 1215 325 47
StuI AGG'CCT
Cuts at: 1517 2071 1517
Size: 554 6287
StyI C'CwwG_G
Cuts at: 513 1065 2004 3217 3382 3703 3768 4280 513
Size: 552 939 1213 165 321 65 512 3074
Fragments arranged by size:
3074 1213 939 552 512 321 165 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2670 4868 4885 5038 5223 5668 325
Size: 1255 1090 2198 17 153 185 445 1498
Fragments arranged by size:
2198 1498 1255 1090 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 3083 3138
Size: 274 80 33 51 1443 432 674 55
Cuts at: 3138 3982 5087 6799 96
Size: 844 1105 1712 138
Fragments arranged by size:
1712 1443 1105 844 674 432 274 138
80 55 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3927 3959 6801 6833 3458
Size: 32 437 32 2842 32 3466
Fragments arranged by size:
3466 2842 437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6841
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2620 2620
Size: 6841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6841
XmaI C'CCGG_G
Cuts at: 1870 4166 1870
Size: 2296 4545
XmnI GAAnn'nnTTC
Cuts at: 2071 3251 4970 2071
Size: 1180 1719 3942
Fragments arranged by size:
3942 1719 1180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 276
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BsiWI BsmBI DraIII EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI