Restriction map for
pFN21AE6722
(Circular) (Six-base) MAPSORT of: pFN21AE6722.seq Check: 9266 from: 1 to: 7000
pFN21AE6722 7000 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2086 4343 1937
Size: 149 2257 4594
Fragments arranged by size:
4594 2257 149
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2554 3691 5063 6447 6715 1632
Size: 922 1137 1372 1384 268 1917
Fragments arranged by size:
1917 1384 1372 1137 922 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 2648 3332 5899 6876 1203
Size: 1445 684 2567 977 1327
Fragments arranged by size:
2567 1445 1327 977 684
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7000
AlwNI CAG_nnn'CTG
Cuts at: 4743 6315 4743
Size: 1572 5428
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 2021 4042 4086 4309 4535 4687 6796 1493
Size: 528 2021 44 223 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 528 226 223 152 44
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 4325 1870
Size: 73 2382 4545
Fragments arranged by size:
4545 2382 73
BamHI G'GATC_C
Cuts at: 4330 4697 4330
Size: 367 6633
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4321 5776 618
Size: 325 217 627 2534 1455 1842
Fragments arranged by size:
2534 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2622 4319 1391
Size: 1231 1697 4072
Fragments arranged by size:
4072 1697 1231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2560 2930 928
Size: 971 661 370 4998
Fragments arranged by size:
4998 971 661 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5191 5225 1903
Size: 34 3254 34 3678
Fragments arranged by size:
3678 3254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4010 4892 6102 3104
Size: 906 882 1210 4002
Fragments arranged by size:
4002 1210 906 882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BfrBI ATG'CAT
Cuts at: 3187 3187
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5611 136
Size: 107 122 71 631 605 3939 1525
Fragments arranged by size:
3939 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2728 3761 3835 6995 2728
Size: 1033 74 3160 2733
Fragments arranged by size:
3160 2733 1033 74
BlpI GC'TnA_GC
Cuts at: 4417 4417
Size: 7000
Bme1580I G_kGCm'C
Cuts at: 1790 5061 6217 1790
Size: 3271 1156 2573
Fragments arranged by size:
3271 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3520 3838 4061
Size: 416 53 83 399 24 2103 318 223
Cuts at: 4061 4731 5689 442
Size: 670 958 1753
Fragments arranged by size:
2103 1753 958 670 416 399 318 223
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3593 1056
Size: 2537 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 2849 3098 3126 4081 4239 5660 1239
Size: 1490 120 249 28 955 158 1421 2579
Fragments arranged by size:
2579 1490 1421 955 249 158 120 28
Bpu10I CC'TnA_GC
Cuts at: 3373 3632 3373
Size: 259 6741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2625 3220 3405 5125 5990 6288 2625
Size: 595 185 1720 865 298 3337
Fragments arranged by size:
3337 1720 865 595 298 185
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2745 2773 3077 3983 5663 882
Size: 617 641 605 28 304 906 1680 2219
Fragments arranged by size:
2219 1680 906 641 617 605 304 28
BsaAI yAC'GTr
Cuts at: 493 1204 2651 4791 493
Size: 711 1447 2140 2702
Fragments arranged by size:
2702 2140 1447 711
BsaBI GATnn'nnATC
Cuts at: 6994 6994
Size: 7000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4807 5189 5824 275
Size: 53 83 186 936 3274 382 635 1451
Fragments arranged by size:
3274 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6775
Fragments arranged by size:
6775 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7000
BseYI C'CCAG_C
Cuts at: 2614 6203 6699 2614
Size: 3589 496 2915
Fragments arranged by size:
3589 2915 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2226 1163
Size: 573 490 5937
Fragments arranged by size:
5937 573 490
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4450 5211 6239 1540
Size: 275 186 2449 761 1028 2301
Fragments arranged by size:
2449 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2857 4225 5061 5146 6217 2857
Size: 1368 836 85 1071 3640
Fragments arranged by size:
3640 1368 1071 836 85
BsmI GAATG_Cn'
Cuts at: 3463 3927 4511 4604 3463
Size: 464 584 93 5859
Fragments arranged by size:
5859 584 464 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3472 3472
Size: 7000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2622 2857 4225 4319 5061 5146 6217
Size: 399 832 235 1368 94 742 85 1071
Cuts at: 6217 1391
Size: 2174
Fragments arranged by size:
2174 1368 1071 832 742 399 235 94
85
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 2530 2862 4889 6619 2530
Size: 332 2027 1730 2911
Fragments arranged by size:
2911 2027 1730 332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4355 844
Size: 3511 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4887 1394
Size: 429 3064 3507
Fragments arranged by size:
3507 3064 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3498 3540 5495 5669 65
Size: 1183 2250 42 1955 174 1396
Fragments arranged by size:
2250 1955 1396 1183 174 42
BsrFI r'CCGG_y
Cuts at: 1700 1810 3904 5644 5831 1700
Size: 110 2094 1740 187 2869
Fragments arranged by size:
2869 2094 1740 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 5060 6072 5060
Size: 1012 5988
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2611 794
Size: 1817 5183
BstBI TT'CG_AA
Cuts at: 5804 5804
Size: 7000
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7000
BstXI CCAn_nnnn'nTGG
Cuts at: 3580 3580
Size: 7000
BstYI r'GATC_y
Cuts at: 1972 2728 3761 3835 4330 4697 5082 5099 6551
Size: 756 1033 74 495 367 385 17 1452
Cuts at: 6551 6637 6649 6995 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1033 756 495 385 367 346
86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 2658 2658
Size: 7000
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 4516 5310 5330 4516
Size: 794 20 6186
Fragments arranged by size:
6186 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 3199 4304 3199
Size: 1105 5895
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 3028 4447
Size: 54 1425 50 275 18 245 953 1419
Cuts at: 4447 5336 6965 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1419 953 889 275 245 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4930 1353
Size: 3577 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4037 5587 5961 6107 387
Size: 1064 138 2432 16 1550 374 146 1280
Fragments arranged by size:
2432 1550 1280 1064 374 146 138 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2554 2729 2849 3098 3126 3691 4081
Size: 393 922 175 120 249 28 565 390
Cuts at: 4081 4239 5063 5660 6447 6715 1239
Size: 158 824 597 787 268 1524
Fragments arranged by size:
1524 922 824 787 597 565 393 390
268 249 175 158 120 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3423 3423
Size: 7000
EcoO109I rG'GnC_Cy
Cuts at: 2894 3293 2894
Size: 399 6601
EcoRI G'AATT_C
Cuts at: 2021 4309 4687 2021
Size: 2288 378 4334
Fragments arranged by size:
4334 2288 378
EcoRV GAT'ATC
Cuts at: 3043 3043
Size: 7000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3780 3783 3780
Size: 3 6997
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 6147 1678
Size: 4469 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 3442 3474 3832
Size: 32 135 32 465 32 1373 32 358
Cuts at: 3832 3864 5641 5673 5715 5747 1373
Size: 32 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 1373 465 358 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2087 4171 4344 4590 669
Size: 988 281 149 2084 173 246 3079
Fragments arranged by size:
3079 2084 988 281 246 173 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2045 2476 2706 2890 6114 6298 1305
Size: 740 431 230 184 3224 184 2007
Fragments arranged by size:
3224 2007 740 431 230 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2077 3030 10
Size: 54 1425 343 245 953 3980
Fragments arranged by size:
3980 1425 953 343 245 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 3119 3337 3468 3488 3578 3690
Size: 648 1293 660 218 131 20 90 112
Cuts at: 3690 4958 5317 5476 518
Size: 1268 359 159 2042
Fragments arranged by size:
2042 1293 1268 660 648 359 218 159
131 112 90 20
MspA1I CmG'CkG
Cuts at: 1606 3569 4416 5093 6241 6486 1606
Size: 1963 847 677 1148 245 2120
Fragments arranged by size:
2120 1963 1148 847 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6448
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 3589 1052
Size: 2537 4463
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NsiI A_TGCA'T
Cuts at: 3189 3189
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 3187 3336 4358 5903 1132
Size: 97 1958 149 1022 1545 2229
Fragments arranged by size:
2229 1958 1545 1022 149 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7000
PciI A'CATG_T
Cuts at: 3332 5899 3332
Size: 2567 4433
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7000
PfoI T'CCnGG_A
Cuts at: 3075 3075
Size: 7000
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PmlI CAC'GTG
Cuts at: 2651 2651
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3293 3293
Size: 7000
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PstI C_TGCA'G
Cuts at: 830 2607 4352 830
Size: 1777 1745 3478
Fragments arranged by size:
3478 1777 1745
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 3569 3569
Size: 7000
SalI G'TCGA_C
Cuts at: 1936 2085 4342 1936
Size: 149 2257 4594
Fragments arranged by size:
4594 2257 149
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 5248 6960 5248
Size: 1712 5288
SfcI C'TryA_G
Cuts at: 826 1045 2484 2603 2817 4348 5483 6164 6355
Size: 219 1439 119 214 1531 1135 681 191
Cuts at: 6355 826
Size: 1471
Fragments arranged by size:
1531 1471 1439 1135 681 219 214 191
119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 4327 1872
Size: 2455 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2640 3235 3420 5104 6005 6267
Size: 197 926 697 595 185 1684 901 262
Cuts at: 6267 820
Size: 1553
Fragments arranged by size:
1684 1553 926 901 697 595 262 197
185
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2756 4439 513
Size: 552 1691 1683 3074
Fragments arranged by size:
3074 1691 1683 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 5027 5044 5197 5382 5827 325
Size: 1255 1533 1914 17 153 185 445 1498
Fragments arranged by size:
1914 1533 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2701 2834 5246
Size: 274 80 33 51 1443 724 133 2412
Cuts at: 5246 6958 96
Size: 1712 138
Fragments arranged by size:
2412 1712 1443 724 274 138 133 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3285 3290 3317 3334 3366 6960 6992 3258
Size: 27 5 27 17 32 3594 32 3266
Fragments arranged by size:
3594 3266 32 32 27 27 17 5
Tth111I GACn'n_nGTC
Cuts at: 1316 3357 3663 3844 1316
Size: 2041 306 181 4472
Fragments arranged by size:
4472 2041 306 181
XbaI T'CTAG_A
Cuts at: 4336 4336
Size: 7000
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 4325 1870
Size: 2455 4545
XmnI GAAnn'nnTTC
Cuts at: 2025 2178 3763 4089 5129 2025
Size: 153 1585 326 1040 3896
Fragments arranged by size:
3896 1585 1040 326 153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI