Restriction map for
pFN21AE6720
(Circular) (Six-base) MAPSORT of: pFN21AE6720.seq Check: 5957 from: 1 to: 6964
pFN21AE6720 6964 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2502 3357 4307 1937
Size: 565 855 950 4594
Fragments arranged by size:
4594 950 855 565
Acc65I G'GTAC_C
Cuts at: 3215 3658 4070 4285 3215
Size: 443 412 215 5894
Fragments arranged by size:
5894 443 412 215
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 3957 5027 6411 6679 1632
Size: 1748 577 1070 1384 268 1917
Fragments arranged by size:
1917 1748 1384 1070 577 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 2288 5863 6840 1203
Size: 1085 3575 977 1327
Fragments arranged by size:
3575 1327 1085 977
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 5693 5693
Size: 6964
AleI CACnn'nnGTG
Cuts at: 1166 3548 1166
Size: 2382 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2319 4157 1184
Size: 1135 1838 3991
Fragments arranged by size:
3991 1838 1135
AlwNI CAG_nnn'CTG
Cuts at: 4707 6279 4707
Size: 1572 5392
ApaLI G'TGCA_C
Cuts at: 5021 6177 5021
Size: 1156 5808
ApoI r'AATT_y
Cuts at: 1493 2665 4273 4499 4651 6760 1493
Size: 1172 1608 226 152 2109 1697
Fragments arranged by size:
2109 1697 1608 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 4289 1870
Size: 73 217 2129 4545
Fragments arranged by size:
4545 2129 217 73
BamHI G'GATC_C
Cuts at: 4294 4661 4294
Size: 367 6597
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2876 3215 3658 3953
Size: 325 217 627 357 732 339 443 295
Cuts at: 3953 4070 4285 5740 618
Size: 117 215 1455 1842
Fragments arranged by size:
1842 1455 732 627 443 357 339 325
295 217 215 117
BanII G_rGCy'C
Cuts at: 1391 4283 1391
Size: 2892 4072
BbeI G_GCGC'C
Cuts at: 2148 2148
Size: 6964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3095 928
Size: 971 1196 4797
Fragments arranged by size:
4797 1196 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5155 5189 1903
Size: 34 3218 34 3678
Fragments arranged by size:
3678 3218 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6964
BfrBI ATG'CAT
Cuts at: 2023 2023
Size: 6964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 5575 136
Size: 107 122 71 631 605 376 3527 1525
Fragments arranged by size:
3527 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 2410 3232 6959 2410
Size: 822 3727 2415
Fragments arranged by size:
3727 2415 822
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 2881 5025 6181 1790
Size: 1091 2144 1156 2573
Fragments arranged by size:
2573 2144 1156 1091
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6964
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2875 3884 4695
Size: 416 53 83 399 24 1458 1009 811
Cuts at: 4695 5653 442
Size: 958 1753
Fragments arranged by size:
1753 1458 1009 958 811 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2333 2333
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2631 5624 1239
Size: 1004 388 2993 2579
Fragments arranged by size:
2993 2579 1004 388
Bpu10I CC'TnA_GC
Cuts at: 2113 2732 2113
Size: 619 6345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3335 3812 5089 5954 6252 3335
Size: 477 1277 865 298 4047
Fragments arranged by size:
4047 1277 865 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5627 882
Size: 617 4128 2219
Fragments arranged by size:
4128 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4755 493
Size: 711 3551 2702
Fragments arranged by size:
3551 2702 711
BsaBI GATnn'nnATC
Cuts at: 2084 6958 2084
Size: 4874 2090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 4771 5153 5788
Size: 53 83 186 936 612 2626 382 635
Cuts at: 5788 275
Size: 1451
Fragments arranged by size:
2626 1451 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5397 5795 6069 6216 6627 5397
Size: 398 274 147 411 5734
Fragments arranged by size:
5734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3222 3252 1178
Size: 30 165 30 1819 30 4890
Fragments arranged by size:
4890 1819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 3855 4211 1186
Size: 1139 1530 356 3939
Fragments arranged by size:
3939 1530 1139 356
BseYI C'CCAG_C
Cuts at: 2034 2437 6167 6663 2034
Size: 403 3730 496 2335
Fragments arranged by size:
3730 2335 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4163 1163
Size: 573 2427 3964
Fragments arranged by size:
3964 2427 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 4414 5175 6203 1540
Size: 275 186 42 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 3192 5025 5110 6181 3192
Size: 1833 85 1071 3975
Fragments arranged by size:
3975 1833 1071 85
BsmI GAATG_Cn'
Cuts at: 2023 2924 3792 4475 4568 2023
Size: 901 868 683 93 4419
Fragments arranged by size:
4419 901 868 683 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2881 3192 4283 5025 5110 6181 1391
Size: 399 1091 311 1091 742 85 1071 2174
Fragments arranged by size:
2174 1091 1091 1071 742 399 311 85
BspEI T'CCGG_A
Cuts at: 6627 6627
Size: 6964
BspHI T'CATG_A
Cuts at: 3769 4853 6583 3769
Size: 1084 1730 4150
Fragments arranged by size:
4150 1730 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 3203 4319 844
Size: 2140 219 1116 3489
Fragments arranged by size:
3489 2140 1116 219
BsrBI CCG'CTC
Cuts at: 1394 1823 2159 4851 1394
Size: 429 336 2692 3507
Fragments arranged by size:
3507 2692 429 336
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 3993 5459 5633 65
Size: 1183 1134 1611 1466 174 1396
Fragments arranged by size:
1611 1466 1396 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 5608 5795 1700
Size: 110 257 3541 187 2869
Fragments arranged by size:
3541 2869 257 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 5024 6036 5024
Size: 1012 5952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2999 794
Size: 2205 4759
BstBI TT'CG_AA
Cuts at: 2058 2635 3784 5768 2058
Size: 577 1149 1984 3254
Fragments arranged by size:
3254 1984 1149 577
BstEII G'GTnAC_C
Cuts at: 1184 2893 1184
Size: 1709 5255
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2437
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2410 2716 3232 4294 4661 5046 5063 6515
Size: 438 306 516 1062 367 385 17 1452
Cuts at: 6515 6601 6613 6959 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1062 516 438 385 367 346
306 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3663 3732 3663
Size: 69 6895
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2568 3420 4078 513
Size: 552 939 39 525 852 658 3399
Fragments arranged by size:
3399 939 852 658 552 525 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 487
Size: 683 56 807 5418
Fragments arranged by size:
5418 807 683 56
BtsI GCAGTG_nn'
Cuts at: 2129 3014 3654 3753 4480 5274 5294 2129
Size: 885 640 99 727 794 20 3799
Fragments arranged by size:
3799 885 794 727 640 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 2491 4268 2491
Size: 1777 5187
DraIII CAC_nnn'GTG
Cuts at: 2946 2946
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3890 5971 809
Size: 507 2574 2081 1802
Fragments arranged by size:
2574 2081 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2267 3510
Size: 54 1425 50 275 18 210 227 1243
Cuts at: 3510 4411 5300 6929 8
Size: 901 889 1629 43
Fragments arranged by size:
1629 1425 1243 901 889 275 227 210
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 4411 1537
Size: 275 228 2371 4090
Fragments arranged by size:
4090 2371 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 3683 3933 4894 1353
Size: 2330 250 961 3423
Fragments arranged by size:
3423 2330 961 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5551 5925 6071 387
Size: 1064 138 3962 374 146 1280
Fragments arranged by size:
3962 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2631 3380 3957 5027 5624 6411
Size: 393 611 388 749 577 1070 597 787
Cuts at: 6411 6679 1239
Size: 268 1524
Fragments arranged by size:
1524 1070 787 749 611 597 577 393
388 268
EcoO109I rG'GnC_Cy
Cuts at: 3883 3883
Size: 6964
EcoRI G'AATT_C
Cuts at: 2665 4273 4651 2665
Size: 1608 378 4978
Fragments arranged by size:
4978 1608 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2396 2861 2396
Size: 465 6499
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 2012 2035 2068 2148 6111 1678
Size: 334 23 33 80 3963 2531
Fragments arranged by size:
3963 2531 334 80 33 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2119 2151 3303 3335 3373
Size: 32 135 32 547 32 1152 32 38
Cuts at: 3373 3405 5605 5637 5679 5711 1373
Size: 32 2200 32 42 32 2626
Fragments arranged by size:
2626 2200 1152 547 135 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4308 4554 669
Size: 988 281 2073 297 246 3079
Fragments arranged by size:
3079 2073 988 297 281 246
HindIII A'AGCT_T
Cuts at: 748 3830 748
Size: 3082 3882
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KasI G'GCGC_C
Cuts at: 2144 2144
Size: 6964
KpnI G_GTAC'C
Cuts at: 3219 3662 4074 4289 3219
Size: 443 412 215 5894
Fragments arranged by size:
5894 443 412 215
MfeI C'AATT_G
Cuts at: 3861 4563 3861
Size: 702 6262
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3208 6078 6262 1305
Size: 1903 2870 184 2007
Fragments arranged by size:
2870 2007 1903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2269 3512 10
Size: 54 1425 343 437 1243 3462
Fragments arranged by size:
3462 1425 1243 437 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 3326 3548 3798 4922 5281 5440
Size: 648 1135 1025 222 250 1124 359 159
Cuts at: 5440 518
Size: 2042
Fragments arranged by size:
2042 1135 1124 1025 648 359 250 222
159
MspA1I CmG'CkG
Cuts at: 1606 2045 4380 5057 6205 6450 1606
Size: 439 2335 677 1148 245 2120
Fragments arranged by size:
2335 2120 1148 677 439 245
NaeI GCC'GGC
Cuts at: 2069 2069
Size: 6964
NarI GG'CG_CC
Cuts at: 2145 2145
Size: 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 2568 4078 513
Size: 552 939 564 1510 3399
Fragments arranged by size:
3399 1510 939 564 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NgoMIV G'CCGG_C
Cuts at: 2067 2067
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 2040 4411 2040
Size: 2371 4593
NruI TCG'CGA
Cuts at: 2018 2018
Size: 6964
NsiI A_TGCA'T
Cuts at: 2025 2025
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 2292 2533 4322 5867 1132
Size: 97 1063 241 1789 1545 2229
Fragments arranged by size:
2229 1789 1545 1063 241 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6964
PciI A'CATG_T
Cuts at: 2288 5863 2288
Size: 3575 3389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6964
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3810 3842 5033 5065 6546 6578 3810
Size: 32 1191 32 1481 32 4196
Fragments arranged by size:
4196 1481 1191 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3883 3883
Size: 6964
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspXI vC'TCGA_Gb
Cuts at: 2160 2160
Size: 6964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2896 2928 2896
Size: 32 6932
PstI C_TGCA'G
Cuts at: 830 3499 4316 830
Size: 2669 817 3478
Fragments arranged by size:
3478 2669 817
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SbfI CC_TGCA'GG
Cuts at: 3499 4316 3499
Size: 817 6147
ScaI AGT'ACT
Cuts at: 5212 6924 5212
Size: 1712 5252
SfcI C'TryA_G
Cuts at: 826 1045 2499 3006 3495 4312 5447 6128 6319
Size: 219 1454 507 489 817 1135 681 191
Cuts at: 6319 826
Size: 1471
Fragments arranged by size:
1471 1454 1135 817 681 507 489 219
191
SfoI GGC'GCC
Cuts at: 2146 2146
Size: 6964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 4291 1872
Size: 2419 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 3350 3827 5068 5969 6231
Size: 197 926 217 1190 477 1241 901 262
Cuts at: 6231 820
Size: 1553
Fragments arranged by size:
1553 1241 1190 926 901 477 262 217
197
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 2470 4888 5
Size: 47 2418 2418 2081
Fragments arranged by size:
2418 2418 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6964
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2568 2899 4078 4403 513
Size: 552 939 144 420 331 1179 325 3074
Fragments arranged by size:
3074 1179 939 552 420 331 325 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 3377 3821 4991 5008 5161 5346
Size: 1255 858 939 444 1170 17 153 185
Cuts at: 5346 5791 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1170 939 858 445 444 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5210 6922 96
Size: 274 80 33 51 1443 3233 1712 138
Fragments arranged by size:
3233 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3492 3524 6924 6956 3492
Size: 32 3400 32 3500
Fragments arranged by size:
3500 3400 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 3964 2470
Size: 1494 5470
XhoI C'TCGA_G
Cuts at: 1943 2160 1943
Size: 217 6747
XmaI C'CCGG_G
Cuts at: 1870 4289 1870
Size: 2419 4545
XmnI GAAnn'nnTTC
Cuts at: 3636 4255 5093 3636
Size: 619 838 5507
Fragments arranged by size:
5507 838 619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
PacI PfoI PmlI PspOMI PvuII RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI